Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2121

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2119 and Pf1N1B4_2120 are separated by 82 nucleotidesPf1N1B4_2120 and Pf1N1B4_2121 are separated by 202 nucleotidesPf1N1B4_2121 and Pf1N1B4_2122 are separated by 25 nucleotides Pf1N1B4_2119 - Exodeoxyribonuclease III (EC 3.1.11.2), at 2,430,582 to 2,431,361 _2119 Pf1N1B4_2120 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 2,431,444 to 2,432,088 _2120 Pf1N1B4_2121 - Acetylglutamate kinase (EC 2.7.2.8), at 2,432,291 to 2,433,196 _2121 Pf1N1B4_2122 - Phosphomannomutase (EC 5.4.2.8) / Phosphoglucomutase (EC 5.4.2.2), at 2,433,222 to 2,434,619 _2122 Position (kb) 2432 2433 2434Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2431.361 kb on + strandat 2431.362 kb on - strandat 2431.362 kb on - strandat 2431.362 kb on - strandat 2431.393 kb on + strandat 2431.394 kb on - strandat 2431.408 kb on + strandat 2431.408 kb on + strandat 2431.408 kb on + strandat 2431.412 kb on + strandat 2431.412 kb on + strandat 2431.412 kb on + strandat 2431.412 kb on + strandat 2431.416 kb on + strandat 2431.416 kb on + strandat 2431.416 kb on + strandat 2431.416 kb on + strandat 2431.416 kb on + strandat 2431.427 kb on + strandat 2431.427 kb on + strandat 2431.427 kb on + strandat 2431.427 kb on + strandat 2431.658 kb on - strand, within Pf1N1B4_2120at 2432.149 kb on - strandat 2432.321 kb on - strandat 2433.219 kb on - strandat 2433.219 kb on - strandat 2433.219 kb on - strandat 2433.377 kb on - strand, within Pf1N1B4_2122at 2433.377 kb on - strand, within Pf1N1B4_2122at 2433.377 kb on - strand, within Pf1N1B4_2122at 2433.377 kb on - strand, within Pf1N1B4_2122at 2433.483 kb on - strand, within Pf1N1B4_2122at 2433.497 kb on + strand, within Pf1N1B4_2122at 2433.497 kb on + strand, within Pf1N1B4_2122at 2433.503 kb on + strand, within Pf1N1B4_2122at 2433.503 kb on + strand, within Pf1N1B4_2122at 2433.503 kb on + strand, within Pf1N1B4_2122at 2433.504 kb on - strand, within Pf1N1B4_2122at 2433.504 kb on - strand, within Pf1N1B4_2122at 2433.504 kb on - strand, within Pf1N1B4_2122at 2433.504 kb on - strand, within Pf1N1B4_2122at 2433.583 kb on + strand, within Pf1N1B4_2122at 2433.583 kb on + strand, within Pf1N1B4_2122at 2433.584 kb on - strand, within Pf1N1B4_2122at 2433.584 kb on - strand, within Pf1N1B4_2122at 2433.584 kb on - strand, within Pf1N1B4_2122at 2433.626 kb on + strand, within Pf1N1B4_2122at 2433.626 kb on + strand, within Pf1N1B4_2122at 2433.626 kb on + strand, within Pf1N1B4_2122at 2433.626 kb on + strand, within Pf1N1B4_2122at 2433.626 kb on + strand, within Pf1N1B4_2122at 2433.627 kb on - strand, within Pf1N1B4_2122at 2433.627 kb on - strand, within Pf1N1B4_2122at 2433.627 kb on - strand, within Pf1N1B4_2122at 2434.138 kb on + strand, within Pf1N1B4_2122at 2434.138 kb on + strand, within Pf1N1B4_2122at 2434.138 kb on + strand, within Pf1N1B4_2122at 2434.139 kb on - strand, within Pf1N1B4_2122at 2434.139 kb on - strand, within Pf1N1B4_2122

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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2,431,361 + -0.0
2,431,362 - +2.3
2,431,362 - +2.3
2,431,362 - +0.9
2,431,393 + +1.6
2,431,394 - -0.4
2,431,408 + +1.5
2,431,408 + +2.4
2,431,408 + +1.9
2,431,412 + +2.6
2,431,412 + +1.7
2,431,412 + +2.2
2,431,412 + +0.3
2,431,416 + +1.3
2,431,416 + +1.1
2,431,416 + +1.5
2,431,416 + +0.3
2,431,416 + +0.5
2,431,427 + +1.2
2,431,427 + +0.6
2,431,427 + +1.9
2,431,427 + +2.4
2,431,658 - Pf1N1B4_2120 0.33 +2.7
2,432,149 - -2.1
2,432,321 - +3.9
2,433,219 - +1.2
2,433,219 - +0.4
2,433,219 - +1.0
2,433,377 - Pf1N1B4_2122 0.11 +1.2
2,433,377 - Pf1N1B4_2122 0.11 +1.8
2,433,377 - Pf1N1B4_2122 0.11 +2.5
2,433,377 - Pf1N1B4_2122 0.11 +1.3
2,433,483 - Pf1N1B4_2122 0.19 +0.6
2,433,497 + Pf1N1B4_2122 0.20 +2.6
2,433,497 + Pf1N1B4_2122 0.20 +1.5
2,433,503 + Pf1N1B4_2122 0.20 +1.9
2,433,503 + Pf1N1B4_2122 0.20 +2.6
2,433,503 + Pf1N1B4_2122 0.20 +2.7
2,433,504 - Pf1N1B4_2122 0.20 +0.6
2,433,504 - Pf1N1B4_2122 0.20 -1.6
2,433,504 - Pf1N1B4_2122 0.20 +1.5
2,433,504 - Pf1N1B4_2122 0.20 -0.4
2,433,583 + Pf1N1B4_2122 0.26 +1.9
2,433,583 + Pf1N1B4_2122 0.26 -0.4
2,433,584 - Pf1N1B4_2122 0.26 -3.2
2,433,584 - Pf1N1B4_2122 0.26 +1.3
2,433,584 - Pf1N1B4_2122 0.26 -0.3
2,433,626 + Pf1N1B4_2122 0.29 +2.4
2,433,626 + Pf1N1B4_2122 0.29 -0.5
2,433,626 + Pf1N1B4_2122 0.29 +1.3
2,433,626 + Pf1N1B4_2122 0.29 -2.1
2,433,626 + Pf1N1B4_2122 0.29 +2.5
2,433,627 - Pf1N1B4_2122 0.29 +0.6
2,433,627 - Pf1N1B4_2122 0.29 -1.8
2,433,627 - Pf1N1B4_2122 0.29 -1.0
2,434,138 + Pf1N1B4_2122 0.66 -0.4
2,434,138 + Pf1N1B4_2122 0.66 -0.8
2,434,138 + Pf1N1B4_2122 0.66 +0.5
2,434,139 - Pf1N1B4_2122 0.66 -1.3
2,434,139 - Pf1N1B4_2122 0.66 +1.6

Or see this region's nucleotide sequence