Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2101 and Pf1N1B4_2102 are separated by 128 nucleotides Pf1N1B4_2102 and Pf1N1B4_2103 are separated by 588 nucleotides
Pf1N1B4_2101 - FIG00953563: hypothetical protein, at 2,412,616 to 2,413,977
_2101
Pf1N1B4_2102 - hypothetical protein, at 2,414,106 to 2,414,486
_2102
Pf1N1B4_2103 - Superfamily II DNA and RNA helicases, at 2,415,075 to 2,415,452
_2103
Position (kb)
2414
2415 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2413.151 kb on + strand, within Pf1N1B4_2101 at 2413.200 kb on + strand, within Pf1N1B4_2101 at 2413.344 kb on + strand, within Pf1N1B4_2101 at 2413.487 kb on + strand, within Pf1N1B4_2101 at 2413.488 kb on - strand, within Pf1N1B4_2101 at 2413.546 kb on + strand, within Pf1N1B4_2101 at 2413.546 kb on + strand, within Pf1N1B4_2101 at 2413.546 kb on + strand, within Pf1N1B4_2101 at 2413.547 kb on - strand, within Pf1N1B4_2101 at 2413.547 kb on - strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.551 kb on - strand, within Pf1N1B4_2101 at 2413.551 kb on - strand, within Pf1N1B4_2101 at 2413.729 kb on + strand, within Pf1N1B4_2101 at 2413.729 kb on + strand, within Pf1N1B4_2101 at 2413.730 kb on - strand, within Pf1N1B4_2101 at 2413.730 kb on - strand, within Pf1N1B4_2101 at 2413.773 kb on + strand, within Pf1N1B4_2101 at 2413.809 kb on + strand, within Pf1N1B4_2101 at 2413.810 kb on - strand, within Pf1N1B4_2101 at 2413.810 kb on - strand, within Pf1N1B4_2101 at 2413.813 kb on + strand, within Pf1N1B4_2101 at 2413.814 kb on - strand, within Pf1N1B4_2101 at 2413.814 kb on - strand, within Pf1N1B4_2101 at 2413.814 kb on - strand, within Pf1N1B4_2101 at 2413.814 kb on - strand, within Pf1N1B4_2101 at 2413.857 kb on + strand at 2413.857 kb on + strand at 2413.857 kb on + strand at 2413.858 kb on - strand at 2413.858 kb on - strand at 2413.955 kb on + strand at 2413.955 kb on + strand at 2413.955 kb on + strand at 2413.956 kb on - strand at 2413.956 kb on - strand at 2413.956 kb on - strand at 2413.956 kb on - strand at 2413.956 kb on - strand at 2413.956 kb on - strand at 2413.993 kb on + strand at 2413.993 kb on + strand at 2413.993 kb on + strand at 2413.993 kb on + strand at 2413.993 kb on + strand at 2413.994 kb on - strand at 2413.994 kb on - strand at 2413.994 kb on - strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.996 kb on - strand at 2413.996 kb on - strand at 2414.032 kb on + strand at 2414.032 kb on + strand at 2414.093 kb on - strand at 2414.107 kb on + strand at 2414.108 kb on - strand at 2414.108 kb on - strand at 2414.147 kb on + strand, within Pf1N1B4_2102 at 2414.147 kb on + strand, within Pf1N1B4_2102 at 2414.148 kb on - strand, within Pf1N1B4_2102 at 2414.148 kb on - strand, within Pf1N1B4_2102 at 2414.492 kb on + strand at 2414.492 kb on + strand at 2414.492 kb on + strand at 2414.492 kb on + strand at 2414.492 kb on + strand at 2414.492 kb on + strand at 2414.493 kb on - strand at 2414.504 kb on + strand at 2414.504 kb on + strand at 2414.504 kb on + strand at 2414.505 kb on - strand at 2414.505 kb on - strand at 2414.511 kb on + strand at 2414.511 kb on + strand at 2414.511 kb on + strand at 2414.512 kb on - strand at 2414.553 kb on + strand at 2414.553 kb on + strand at 2414.553 kb on + strand at 2414.554 kb on - strand at 2414.554 kb on - strand at 2414.554 kb on - strand at 2414.554 kb on - strand at 2414.554 kb on - strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.634 kb on + strand at 2414.634 kb on + strand at 2414.634 kb on + strand at 2414.635 kb on - strand at 2414.635 kb on - strand at 2414.635 kb on - strand at 2414.635 kb on - strand at 2414.653 kb on + strand at 2414.654 kb on - strand at 2414.750 kb on + strand at 2414.750 kb on + strand at 2414.750 kb on + strand at 2414.751 kb on - strand at 2414.751 kb on - strand at 2414.751 kb on - strand at 2414.839 kb on - strand at 2414.839 kb on - strand at 2414.862 kb on + strand at 2414.862 kb on + strand at 2414.995 kb on + strand at 2415.034 kb on - strand at 2415.134 kb on + strand, within Pf1N1B4_2103 at 2415.134 kb on + strand, within Pf1N1B4_2103 at 2415.135 kb on - strand, within Pf1N1B4_2103 at 2415.184 kb on + strand, within Pf1N1B4_2103 at 2415.184 kb on + strand, within Pf1N1B4_2103 at 2415.184 kb on + strand, within Pf1N1B4_2103 at 2415.184 kb on + strand, within Pf1N1B4_2103 at 2415.185 kb on - strand, within Pf1N1B4_2103 at 2415.239 kb on + strand, within Pf1N1B4_2103 at 2415.240 kb on - strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.259 kb on - strand, within Pf1N1B4_2103 at 2415.259 kb on - strand, within Pf1N1B4_2103 at 2415.344 kb on + strand, within Pf1N1B4_2103 at 2415.344 kb on + strand, within Pf1N1B4_2103 at 2415.345 kb on - strand, within Pf1N1B4_2103 at 2415.345 kb on - strand, within Pf1N1B4_2103 at 2415.345 kb on - strand, within Pf1N1B4_2103 at 2415.423 kb on + strand at 2415.423 kb on + strand at 2415.423 kb on + strand at 2415.424 kb on - strand at 2415.434 kb on + strand at 2415.434 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 2,413,151 + Pf1N1B4_2101 0.39 +1.2 2,413,200 + Pf1N1B4_2101 0.43 -0.0 2,413,344 + Pf1N1B4_2101 0.53 -2.1 2,413,487 + Pf1N1B4_2101 0.64 -0.4 2,413,488 - Pf1N1B4_2101 0.64 -0.8 2,413,546 + Pf1N1B4_2101 0.68 -1.3 2,413,546 + Pf1N1B4_2101 0.68 +1.4 2,413,546 + Pf1N1B4_2101 0.68 -2.8 2,413,547 - Pf1N1B4_2101 0.68 -1.4 2,413,547 - Pf1N1B4_2101 0.68 +3.3 2,413,550 + Pf1N1B4_2101 0.69 +0.3 2,413,550 + Pf1N1B4_2101 0.69 -2.8 2,413,550 + Pf1N1B4_2101 0.69 +1.7 2,413,550 + Pf1N1B4_2101 0.69 +0.0 2,413,550 + Pf1N1B4_2101 0.69 +1.1 2,413,550 + Pf1N1B4_2101 0.69 +1.1 2,413,550 + Pf1N1B4_2101 0.69 -1.3 2,413,550 + Pf1N1B4_2101 0.69 -0.9 2,413,550 + Pf1N1B4_2101 0.69 -0.1 2,413,550 + Pf1N1B4_2101 0.69 +0.1 2,413,550 + Pf1N1B4_2101 0.69 -0.9 2,413,550 + Pf1N1B4_2101 0.69 -1.9 2,413,550 + Pf1N1B4_2101 0.69 -0.8 2,413,550 + Pf1N1B4_2101 0.69 +0.6 2,413,550 + +1.1 2,413,550 + Pf1N1B4_2101 0.69 +1.6 2,413,550 + Pf1N1B4_2101 0.69 +1.3 2,413,551 - Pf1N1B4_2101 0.69 -1.0 2,413,551 - Pf1N1B4_2101 0.69 -0.3 2,413,729 + Pf1N1B4_2101 0.82 -1.5 2,413,729 + Pf1N1B4_2101 0.82 +1.5 2,413,730 - Pf1N1B4_2101 0.82 +0.6 2,413,730 - Pf1N1B4_2101 0.82 +2.0 2,413,773 + Pf1N1B4_2101 0.85 +0.4 2,413,809 + Pf1N1B4_2101 0.88 +1.4 2,413,810 - Pf1N1B4_2101 0.88 -1.8 2,413,810 - Pf1N1B4_2101 0.88 -1.9 2,413,813 + Pf1N1B4_2101 0.88 -0.9 2,413,814 - Pf1N1B4_2101 0.88 -0.4 2,413,814 - Pf1N1B4_2101 0.88 +0.8 2,413,814 - Pf1N1B4_2101 0.88 +0.6 2,413,814 - Pf1N1B4_2101 0.88 -3.0 2,413,857 + -1.0 2,413,857 + +0.4 2,413,857 + -2.0 2,413,858 - +0.8 2,413,858 - -0.8 2,413,955 + -2.9 2,413,955 + +1.1 2,413,955 + +1.4 2,413,956 - -1.8 2,413,956 - +1.3 2,413,956 - -2.1 2,413,956 - -1.0 2,413,956 - +0.2 2,413,956 - -1.9 2,413,993 + -0.6 2,413,993 + +0.3 2,413,993 + +1.1 2,413,993 + +2.9 2,413,993 + -2.2 2,413,994 - +0.3 2,413,994 - +1.8 2,413,994 - +0.1 2,413,995 + +0.4 2,413,995 + -2.9 2,413,995 + -0.5 2,413,995 + -0.1 2,413,995 + +1.2 2,413,995 + +3.0 2,413,995 + +0.4 2,413,996 - -0.6 2,413,996 - +0.4 2,414,032 + -0.9 2,414,032 + +0.2 2,414,093 - -0.8 2,414,107 + +1.7 2,414,108 - +0.2 2,414,108 - +0.3 2,414,147 + Pf1N1B4_2102 0.11 -1.5 2,414,147 + Pf1N1B4_2102 0.11 -1.0 2,414,148 - Pf1N1B4_2102 0.11 -0.7 2,414,148 - Pf1N1B4_2102 0.11 -2.5 2,414,492 + +0.2 2,414,492 + -0.4 2,414,492 + -1.8 2,414,492 + +1.3 2,414,492 + -1.1 2,414,492 + -1.0 2,414,493 - -1.4 2,414,504 + +0.5 2,414,504 + +0.2 2,414,504 + +1.0 2,414,505 - +1.3 2,414,505 - -0.3 2,414,511 + -2.1 2,414,511 + -0.0 2,414,511 + +0.6 2,414,512 - -1.6 2,414,553 + -0.4 2,414,553 + +2.0 2,414,553 + -0.2 2,414,554 - -2.0 2,414,554 - -1.4 2,414,554 - -2.3 2,414,554 - -0.4 2,414,554 - +0.9 2,414,555 + +1.9 2,414,555 + -1.5 2,414,555 + -2.2 2,414,555 + -0.5 2,414,555 + +2.2 2,414,555 + +1.2 2,414,555 + -2.4 2,414,555 + -2.4 2,414,555 + -1.0 2,414,555 + +0.6 2,414,555 + -0.0 2,414,555 + +0.8 2,414,555 + -1.1 2,414,555 + -2.6 2,414,555 + -1.4 2,414,556 - -0.3 2,414,556 - -0.8 2,414,556 - -2.4 2,414,556 - -2.5 2,414,556 - -2.0 2,414,556 - -0.3 2,414,556 - -1.4 2,414,556 - -2.7 2,414,556 - -0.6 2,414,556 - -3.8 2,414,556 - -2.8 2,414,556 - +1.8 2,414,556 - -2.1 2,414,556 - -1.6 2,414,556 - -1.3 2,414,556 - -3.2 2,414,556 - -1.0 2,414,556 - -3.7 2,414,556 - -1.2 2,414,556 - +0.3 2,414,556 - -3.2 2,414,556 - -1.4 2,414,556 - -1.7 2,414,556 - -0.9 2,414,556 - -0.3 2,414,556 - -1.6 2,414,634 + +1.4 2,414,634 + -3.2 2,414,634 + +0.1 2,414,635 - -0.5 2,414,635 - -0.3 2,414,635 - +1.1 2,414,635 - -1.0 2,414,653 + +0.1 2,414,654 - -0.8 2,414,750 + +0.7 2,414,750 + +0.5 2,414,750 + -1.8 2,414,751 - -0.6 2,414,751 - -1.7 2,414,751 - +1.1 2,414,839 - +2.9 2,414,839 - +1.5 2,414,862 + +0.3 2,414,862 + -1.0 2,414,995 + -0.0 2,415,034 - +0.2 2,415,134 + Pf1N1B4_2103 0.16 -0.7 2,415,134 + Pf1N1B4_2103 0.16 -0.4 2,415,135 - Pf1N1B4_2103 0.16 -1.9 2,415,184 + Pf1N1B4_2103 0.29 -0.0 2,415,184 + Pf1N1B4_2103 0.29 -1.7 2,415,184 + Pf1N1B4_2103 0.29 +0.5 2,415,184 + Pf1N1B4_2103 0.29 -1.4 2,415,185 - Pf1N1B4_2103 0.29 +1.1 2,415,239 + Pf1N1B4_2103 0.43 +0.3 2,415,240 - Pf1N1B4_2103 0.44 -1.3 2,415,258 + Pf1N1B4_2103 0.48 -0.0 2,415,258 + Pf1N1B4_2103 0.48 +1.2 2,415,258 + Pf1N1B4_2103 0.48 -3.1 2,415,258 + Pf1N1B4_2103 0.48 -0.0 2,415,258 + Pf1N1B4_2103 0.48 -2.4 2,415,258 + Pf1N1B4_2103 0.48 -1.6 2,415,258 + Pf1N1B4_2103 0.48 +1.2 2,415,258 + Pf1N1B4_2103 0.48 -0.4 2,415,259 - Pf1N1B4_2103 0.49 -0.7 2,415,259 - Pf1N1B4_2103 0.49 +0.7 2,415,344 + Pf1N1B4_2103 0.71 +0.3 2,415,344 + Pf1N1B4_2103 0.71 +1.2 2,415,345 - Pf1N1B4_2103 0.71 +0.6 2,415,345 - Pf1N1B4_2103 0.71 +0.4 2,415,345 - Pf1N1B4_2103 0.71 +0.5 2,415,423 + -1.0 2,415,423 + +0.9 2,415,423 + -0.0 2,415,424 - +0.3 2,415,434 + +0.2 2,415,434 + -2.3
Or see this region's nucleotide sequence