Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2046 and Pf1N1B4_2047 overlap by 1 nucleotides Pf1N1B4_2047 and Pf1N1B4_2048 are separated by 186 nucleotides Pf1N1B4_2048 and Pf1N1B4_2049 overlap by 4 nucleotides Pf1N1B4_2049 and Pf1N1B4_2050 are separated by 19 nucleotides
Pf1N1B4_2046 - 4-hydroxybenzoate polyprenyltransferase (EC 2.5.1.39), at 2,334,561 to 2,335,451
_2046
Pf1N1B4_2047 - Chorismate--pyruvate lyase (EC 4.1.3.40), at 2,335,451 to 2,335,885
_2047
Pf1N1B4_2048 - hypothetical protein, at 2,336,072 to 2,336,218
_2048
Pf1N1B4_2049 - Rubredoxin, at 2,336,215 to 2,336,382
_2049
Pf1N1B4_2050 - Rubredoxin-NAD(+) reductase (EC 1.18.1.1), at 2,336,402 to 2,337,550
_2050
Position (kb)
2335
2336 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 2334.484 kb on + strand at 2334.491 kb on + strand at 2334.574 kb on + strand at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.768 kb on - strand, within Pf1N1B4_2047 at 2335.768 kb on - strand at 2335.922 kb on + strand at 2335.922 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.937 kb on - strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.053 kb on - strand at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.208 kb on + strand at 2336.209 kb on - strand at 2336.209 kb on - strand at 2336.251 kb on + strand, within Pf1N1B4_2049 at 2336.251 kb on + strand, within Pf1N1B4_2049 at 2336.252 kb on - strand at 2336.252 kb on - strand, within Pf1N1B4_2049 at 2336.252 kb on - strand at 2336.252 kb on - strand, within Pf1N1B4_2049 at 2336.380 kb on + strand at 2336.380 kb on + strand at 2336.380 kb on + strand at 2336.381 kb on - strand at 2336.381 kb on - strand at 2336.381 kb on - strand at 2336.381 kb on - strand at 2336.522 kb on + strand, within Pf1N1B4_2050 at 2336.560 kb on + strand, within Pf1N1B4_2050 at 2336.560 kb on + strand, within Pf1N1B4_2050 at 2336.560 kb on + strand, within Pf1N1B4_2050 at 2336.665 kb on + strand, within Pf1N1B4_2050 at 2336.699 kb on + strand, within Pf1N1B4_2050 at 2336.699 kb on + strand at 2336.699 kb on + strand, within Pf1N1B4_2050 at 2336.700 kb on - strand, within Pf1N1B4_2050 at 2336.700 kb on - strand, within Pf1N1B4_2050 at 2336.798 kb on + strand, within Pf1N1B4_2050 at 2336.799 kb on - strand, within Pf1N1B4_2050
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 2,334,484 + -3.0 2,334,491 + +0.1 2,334,574 + -1.3 2,335,687 + Pf1N1B4_2047 0.54 +0.1 2,335,687 + Pf1N1B4_2047 0.54 +2.3 2,335,687 + Pf1N1B4_2047 0.54 +3.0 2,335,687 + Pf1N1B4_2047 0.54 +3.1 2,335,687 + Pf1N1B4_2047 0.54 +2.2 2,335,767 + Pf1N1B4_2047 0.73 +1.7 2,335,767 + Pf1N1B4_2047 0.73 +2.0 2,335,767 + Pf1N1B4_2047 0.73 +3.0 2,335,767 + Pf1N1B4_2047 0.73 +6.2 2,335,767 + Pf1N1B4_2047 0.73 +2.9 2,335,768 - Pf1N1B4_2047 0.73 -0.3 2,335,768 - -0.7 2,335,922 + +2.7 2,335,922 + +1.5 2,335,936 + +5.0 2,335,936 + +2.3 2,335,936 + +2.5 2,335,936 + +3.9 2,335,936 + +3.3 2,335,937 - +0.6 2,336,052 + +2.5 2,336,052 + +2.6 2,336,052 + +3.5 2,336,052 + +2.3 2,336,052 + +1.7 2,336,052 + +2.3 2,336,053 - +1.0 2,336,130 + Pf1N1B4_2048 0.39 -0.2 2,336,130 + Pf1N1B4_2048 0.39 -0.4 2,336,130 + Pf1N1B4_2048 0.39 +1.6 2,336,130 + Pf1N1B4_2048 0.39 +1.7 2,336,208 + -2.1 2,336,209 - +0.2 2,336,209 - +1.7 2,336,251 + Pf1N1B4_2049 0.21 -1.3 2,336,251 + Pf1N1B4_2049 0.21 -1.3 2,336,252 - +1.0 2,336,252 - Pf1N1B4_2049 0.22 +0.9 2,336,252 - +0.0 2,336,252 - Pf1N1B4_2049 0.22 -1.2 2,336,380 + -0.2 2,336,380 + -1.0 2,336,380 + -2.6 2,336,381 - +0.0 2,336,381 - +0.3 2,336,381 - +2.4 2,336,381 - +0.3 2,336,522 + Pf1N1B4_2050 0.10 +0.1 2,336,560 + Pf1N1B4_2050 0.14 +1.7 2,336,560 + Pf1N1B4_2050 0.14 -1.3 2,336,560 + Pf1N1B4_2050 0.14 -1.7 2,336,665 + Pf1N1B4_2050 0.23 +1.3 2,336,699 + Pf1N1B4_2050 0.26 +0.4 2,336,699 + -0.4 2,336,699 + Pf1N1B4_2050 0.26 -0.5 2,336,700 - Pf1N1B4_2050 0.26 -0.1 2,336,700 - Pf1N1B4_2050 0.26 +0.2 2,336,798 + Pf1N1B4_2050 0.34 -1.8 2,336,799 - Pf1N1B4_2050 0.35 +0.6
Or see this region's nucleotide sequence