Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1918

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1917 and Pf1N1B4_1918 are separated by 40 nucleotidesPf1N1B4_1918 and Pf1N1B4_1919 are separated by 81 nucleotides Pf1N1B4_1917 - Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-), at 2,191,515 to 2,192,402 _1917 Pf1N1B4_1918 - DNA-3-methyladenine glycosylase (EC 3.2.2.20), at 2,192,443 to 2,193,000 _1918 Pf1N1B4_1919 - Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14), at 2,193,082 to 2,194,035 _1919 Position (kb) 2192 2193 2194Strain fitness (log2 ratio) -2 -1 0 1 2at 2191.464 kb on + strandat 2191.520 kb on - strandat 2192.397 kb on - strandat 2192.445 kb on - strandat 2192.445 kb on - strandat 2192.954 kb on + strandat 2192.954 kb on + strandat 2192.963 kb on + strandat 2192.963 kb on + strandat 2192.963 kb on + strandat 2192.963 kb on + strandat 2192.963 kb on + strandat 2192.963 kb on + strandat 2192.964 kb on - strandat 2192.964 kb on - strandat 2192.964 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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2,191,464 + +0.2
2,191,520 - +2.0
2,192,397 - +2.3
2,192,445 - -1.8
2,192,445 - -2.2
2,192,954 + -0.2
2,192,954 + +0.3
2,192,963 + -0.5
2,192,963 + +1.0
2,192,963 + -2.3
2,192,963 + +1.0
2,192,963 + +2.2
2,192,963 + -1.8
2,192,964 - +0.1
2,192,964 - +0.2
2,192,964 - +0.9

Or see this region's nucleotide sequence