Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1896 and Pf1N1B4_1897 are separated by 348 nucleotides Pf1N1B4_1897 and Pf1N1B4_1898 are separated by 86 nucleotides
Pf1N1B4_1896 - Sodium-dependent phosphate transporter, at 2,169,240 to 2,170,898
_1896
Pf1N1B4_1897 - Integral membrane protein TerC, at 2,171,247 to 2,172,014
_1897
Pf1N1B4_1898 - Uncharacterized transporter, similarity to citrate transporter, at 2,172,101 to 2,173,408
_1898
Position (kb)
2171
2172
2173 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2170.343 kb on - strand, within Pf1N1B4_1896 at 2170.343 kb on - strand, within Pf1N1B4_1896 at 2170.436 kb on + strand, within Pf1N1B4_1896 at 2170.437 kb on - strand, within Pf1N1B4_1896 at 2170.781 kb on + strand at 2170.813 kb on + strand at 2170.814 kb on - strand at 2170.898 kb on + strand at 2170.898 kb on + strand at 2170.899 kb on - strand at 2170.959 kb on + strand at 2170.960 kb on - strand at 2170.968 kb on + strand at 2170.968 kb on + strand at 2170.968 kb on + strand at 2170.969 kb on - strand at 2170.969 kb on - strand at 2170.969 kb on - strand at 2170.969 kb on - strand at 2170.969 kb on - strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.972 kb on + strand at 2170.973 kb on - strand at 2170.973 kb on - strand at 2170.973 kb on - strand at 2170.973 kb on - strand at 2170.973 kb on - strand at 2170.973 kb on - strand at 2170.973 kb on - strand at 2170.973 kb on - strand at 2170.973 kb on - strand at 2170.979 kb on + strand at 2170.979 kb on + strand at 2170.979 kb on + strand at 2170.979 kb on + strand at 2170.979 kb on + strand at 2170.980 kb on - strand at 2170.980 kb on - strand at 2170.980 kb on - strand at 2170.980 kb on - strand at 2171.012 kb on + strand at 2171.013 kb on - strand at 2171.013 kb on - strand at 2171.013 kb on - strand at 2171.013 kb on - strand at 2171.016 kb on + strand at 2171.016 kb on + strand at 2171.017 kb on - strand at 2171.017 kb on - strand at 2171.017 kb on - strand at 2171.081 kb on - strand at 2171.140 kb on + strand at 2171.140 kb on + strand at 2171.141 kb on - strand at 2171.142 kb on + strand at 2171.142 kb on + strand at 2171.142 kb on + strand at 2171.142 kb on + strand at 2171.142 kb on + strand at 2171.142 kb on + strand at 2171.142 kb on + strand at 2171.142 kb on + strand at 2171.142 kb on + strand at 2171.142 kb on + strand at 2171.142 kb on + strand at 2171.143 kb on - strand at 2171.143 kb on - strand at 2171.143 kb on - strand at 2171.143 kb on - strand at 2171.143 kb on - strand at 2171.143 kb on - strand at 2171.143 kb on - strand at 2171.143 kb on - strand at 2171.143 kb on - strand at 2171.143 kb on - strand at 2171.143 kb on - strand at 2171.143 kb on - strand at 2171.158 kb on + strand at 2171.158 kb on + strand at 2171.158 kb on + strand at 2171.159 kb on - strand at 2171.368 kb on + strand, within Pf1N1B4_1897 at 2171.368 kb on + strand, within Pf1N1B4_1897 at 2171.369 kb on - strand, within Pf1N1B4_1897 at 2171.369 kb on - strand, within Pf1N1B4_1897 at 2171.369 kb on - strand, within Pf1N1B4_1897 at 2171.369 kb on - strand, within Pf1N1B4_1897 at 2171.374 kb on + strand, within Pf1N1B4_1897 at 2171.374 kb on + strand, within Pf1N1B4_1897 at 2171.374 kb on + strand, within Pf1N1B4_1897 at 2171.374 kb on + strand, within Pf1N1B4_1897 at 2171.375 kb on - strand, within Pf1N1B4_1897 at 2171.375 kb on - strand, within Pf1N1B4_1897 at 2171.375 kb on - strand, within Pf1N1B4_1897 at 2171.375 kb on - strand, within Pf1N1B4_1897 at 2171.375 kb on - strand, within Pf1N1B4_1897 at 2171.375 kb on - strand, within Pf1N1B4_1897 at 2171.550 kb on + strand at 2171.632 kb on + strand, within Pf1N1B4_1897 at 2171.633 kb on - strand, within Pf1N1B4_1897 at 2171.633 kb on - strand, within Pf1N1B4_1897 at 2171.698 kb on + strand, within Pf1N1B4_1897 at 2171.698 kb on + strand, within Pf1N1B4_1897 at 2171.698 kb on + strand, within Pf1N1B4_1897 at 2171.698 kb on + strand, within Pf1N1B4_1897 at 2171.699 kb on - strand, within Pf1N1B4_1897 at 2171.699 kb on - strand, within Pf1N1B4_1897 at 2171.699 kb on - strand, within Pf1N1B4_1897 at 2171.699 kb on - strand, within Pf1N1B4_1897 at 2172.018 kb on + strand at 2172.018 kb on + strand at 2172.018 kb on + strand at 2172.038 kb on + strand at 2172.038 kb on + strand at 2172.039 kb on - strand at 2172.102 kb on + strand at 2172.102 kb on + strand at 2172.103 kb on - strand at 2172.103 kb on - strand at 2172.104 kb on + strand at 2172.104 kb on + strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.105 kb on - strand at 2172.152 kb on + strand at 2172.153 kb on - strand at 2172.238 kb on - strand, within Pf1N1B4_1898 at 2172.238 kb on - strand, within Pf1N1B4_1898 at 2172.238 kb on - strand, within Pf1N1B4_1898 at 2172.238 kb on - strand at 2172.238 kb on - strand, within Pf1N1B4_1898 at 2172.317 kb on - strand, within Pf1N1B4_1898 at 2172.322 kb on - strand, within Pf1N1B4_1898 at 2172.345 kb on - strand, within Pf1N1B4_1898 at 2172.345 kb on - strand, within Pf1N1B4_1898 at 2172.345 kb on - strand, within Pf1N1B4_1898 at 2172.360 kb on + strand, within Pf1N1B4_1898 at 2172.364 kb on - strand, within Pf1N1B4_1898 at 2172.364 kb on - strand, within Pf1N1B4_1898 at 2172.364 kb on - strand, within Pf1N1B4_1898 at 2172.364 kb on - strand, within Pf1N1B4_1898 at 2172.364 kb on - strand, within Pf1N1B4_1898 at 2172.364 kb on - strand, within Pf1N1B4_1898 at 2172.416 kb on - strand, within Pf1N1B4_1898 at 2172.518 kb on - strand, within Pf1N1B4_1898 at 2172.551 kb on + strand, within Pf1N1B4_1898 at 2172.551 kb on + strand, within Pf1N1B4_1898 at 2172.604 kb on - strand, within Pf1N1B4_1898 at 2172.825 kb on + strand, within Pf1N1B4_1898 at 2172.825 kb on + strand at 2172.825 kb on + strand, within Pf1N1B4_1898 at 2172.826 kb on - strand, within Pf1N1B4_1898 at 2172.831 kb on + strand, within Pf1N1B4_1898 at 2172.831 kb on + strand, within Pf1N1B4_1898 at 2172.831 kb on + strand, within Pf1N1B4_1898 at 2172.832 kb on - strand, within Pf1N1B4_1898 at 2172.832 kb on - strand, within Pf1N1B4_1898
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 2,170,343 - Pf1N1B4_1896 0.66 -0.2 2,170,343 - Pf1N1B4_1896 0.66 +0.7 2,170,436 + Pf1N1B4_1896 0.72 +0.4 2,170,437 - Pf1N1B4_1896 0.72 -1.8 2,170,781 + -0.8 2,170,813 + +0.7 2,170,814 - +1.0 2,170,898 + -1.9 2,170,898 + -1.3 2,170,899 - -0.1 2,170,959 + -1.6 2,170,960 - +1.0 2,170,968 + +1.0 2,170,968 + -1.3 2,170,968 + +0.0 2,170,969 - -1.3 2,170,969 - +0.6 2,170,969 - +0.5 2,170,969 - +1.1 2,170,969 - -1.3 2,170,972 + -1.8 2,170,972 + -0.3 2,170,972 + +1.5 2,170,972 + +0.1 2,170,972 + -1.5 2,170,972 + +1.0 2,170,972 + -0.2 2,170,972 + -1.4 2,170,972 + -1.2 2,170,972 + -0.5 2,170,972 + -1.7 2,170,972 + -0.9 2,170,972 + -0.1 2,170,972 + -0.6 2,170,973 - -1.2 2,170,973 - -1.8 2,170,973 - +0.6 2,170,973 - +0.4 2,170,973 - -1.9 2,170,973 - -2.2 2,170,973 - +1.3 2,170,973 - -1.0 2,170,973 - -0.7 2,170,979 + +0.5 2,170,979 + +1.4 2,170,979 + -1.2 2,170,979 + -1.9 2,170,979 + -2.9 2,170,980 - +2.6 2,170,980 - +0.4 2,170,980 - -0.5 2,170,980 - -3.1 2,171,012 + -1.6 2,171,013 - +0.4 2,171,013 - -2.2 2,171,013 - -2.4 2,171,013 - +0.4 2,171,016 + -0.1 2,171,016 + -1.4 2,171,017 - -0.6 2,171,017 - -0.6 2,171,017 - +1.0 2,171,081 - -0.9 2,171,140 + +0.3 2,171,140 + -1.3 2,171,141 - +0.4 2,171,142 + +1.3 2,171,142 + -1.2 2,171,142 + +0.8 2,171,142 + +1.6 2,171,142 + +0.5 2,171,142 + -2.0 2,171,142 + +0.1 2,171,142 + -2.7 2,171,142 + -1.0 2,171,142 + -0.8 2,171,142 + +1.0 2,171,143 - -0.8 2,171,143 - +0.6 2,171,143 - +1.2 2,171,143 - -2.5 2,171,143 - -0.9 2,171,143 - -0.4 2,171,143 - +1.8 2,171,143 - -0.5 2,171,143 - +0.4 2,171,143 - -0.1 2,171,143 - -0.3 2,171,143 - -2.1 2,171,158 + -1.5 2,171,158 + -1.8 2,171,158 + -0.8 2,171,159 - +0.0 2,171,368 + Pf1N1B4_1897 0.16 -2.3 2,171,368 + Pf1N1B4_1897 0.16 +0.7 2,171,369 - Pf1N1B4_1897 0.16 -3.3 2,171,369 - Pf1N1B4_1897 0.16 -0.9 2,171,369 - Pf1N1B4_1897 0.16 -2.8 2,171,369 - Pf1N1B4_1897 0.16 -0.5 2,171,374 + Pf1N1B4_1897 0.17 -0.5 2,171,374 + Pf1N1B4_1897 0.17 -2.0 2,171,374 + Pf1N1B4_1897 0.17 +1.1 2,171,374 + Pf1N1B4_1897 0.17 -1.3 2,171,375 - Pf1N1B4_1897 0.17 +0.4 2,171,375 - Pf1N1B4_1897 0.17 -2.6 2,171,375 - Pf1N1B4_1897 0.17 -0.2 2,171,375 - Pf1N1B4_1897 0.17 -1.8 2,171,375 - Pf1N1B4_1897 0.17 -0.7 2,171,375 - Pf1N1B4_1897 0.17 -1.3 2,171,550 + +0.2 2,171,632 + Pf1N1B4_1897 0.50 -1.9 2,171,633 - Pf1N1B4_1897 0.50 -1.4 2,171,633 - Pf1N1B4_1897 0.50 -1.9 2,171,698 + Pf1N1B4_1897 0.59 +0.3 2,171,698 + Pf1N1B4_1897 0.59 -2.0 2,171,698 + Pf1N1B4_1897 0.59 -3.2 2,171,698 + Pf1N1B4_1897 0.59 -1.2 2,171,699 - Pf1N1B4_1897 0.59 -0.8 2,171,699 - Pf1N1B4_1897 0.59 -2.1 2,171,699 - Pf1N1B4_1897 0.59 -1.7 2,171,699 - Pf1N1B4_1897 0.59 -1.5 2,172,018 + -3.1 2,172,018 + -1.9 2,172,018 + -0.2 2,172,038 + -0.4 2,172,038 + -1.1 2,172,039 - +0.4 2,172,102 + -0.6 2,172,102 + -0.5 2,172,103 - -0.7 2,172,103 - -0.4 2,172,104 + +0.7 2,172,104 + -1.0 2,172,105 - -1.2 2,172,105 - -2.0 2,172,105 - +0.7 2,172,105 - -2.0 2,172,105 - -0.4 2,172,105 - +0.3 2,172,105 - -0.9 2,172,105 - -0.3 2,172,105 - -0.2 2,172,105 - +0.4 2,172,105 - +0.4 2,172,105 - +0.4 2,172,105 - -0.9 2,172,105 - +1.3 2,172,105 - +0.9 2,172,105 - +1.1 2,172,105 - -2.6 2,172,152 + +0.9 2,172,153 - -2.1 2,172,238 - Pf1N1B4_1898 0.10 -1.0 2,172,238 - Pf1N1B4_1898 0.10 -0.4 2,172,238 - Pf1N1B4_1898 0.10 +1.0 2,172,238 - -0.6 2,172,238 - Pf1N1B4_1898 0.10 +1.8 2,172,317 - Pf1N1B4_1898 0.17 +1.6 2,172,322 - Pf1N1B4_1898 0.17 -0.3 2,172,345 - Pf1N1B4_1898 0.19 +0.7 2,172,345 - Pf1N1B4_1898 0.19 +2.2 2,172,345 - Pf1N1B4_1898 0.19 -2.7 2,172,360 + Pf1N1B4_1898 0.20 +1.3 2,172,364 - Pf1N1B4_1898 0.20 -0.4 2,172,364 - Pf1N1B4_1898 0.20 -1.1 2,172,364 - Pf1N1B4_1898 0.20 -2.4 2,172,364 - Pf1N1B4_1898 0.20 +0.9 2,172,364 - Pf1N1B4_1898 0.20 +1.1 2,172,364 - Pf1N1B4_1898 0.20 -2.8 2,172,416 - Pf1N1B4_1898 0.24 +0.2 2,172,518 - Pf1N1B4_1898 0.32 -2.6 2,172,551 + Pf1N1B4_1898 0.34 -1.4 2,172,551 + Pf1N1B4_1898 0.34 +1.4 2,172,604 - Pf1N1B4_1898 0.38 -1.1 2,172,825 + Pf1N1B4_1898 0.55 -2.1 2,172,825 + -0.0 2,172,825 + Pf1N1B4_1898 0.55 +0.4 2,172,826 - Pf1N1B4_1898 0.55 -0.1 2,172,831 + Pf1N1B4_1898 0.56 +0.6 2,172,831 + Pf1N1B4_1898 0.56 -0.6 2,172,831 + Pf1N1B4_1898 0.56 +1.7 2,172,832 - Pf1N1B4_1898 0.56 -0.9 2,172,832 - Pf1N1B4_1898 0.56 +2.3
Or see this region's nucleotide sequence