Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1335 and Pf1N1B4_1336 are separated by 66 nucleotides Pf1N1B4_1336 and Pf1N1B4_1337 are separated by 84 nucleotides Pf1N1B4_1337 and Pf1N1B4_1338 are separated by 196 nucleotides
Pf1N1B4_1335 - Copper metallochaperone, bacterial analog of Cox17 protein, at 1,529,876 to 1,530,355
_1335
Pf1N1B4_1336 - Putative multicopper oxidases, at 1,530,422 to 1,530,826
_1336
Pf1N1B4_1337 - Outer membrane receptor proteins, mostly Fe transport, at 1,530,911 to 1,533,028
_1337
Pf1N1B4_1338 - Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB, at 1,533,225 to 1,534,601
_1338
Position (kb)
1530
1531
1532
1533
1534 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.943 kb on - strand, within Pf1N1B4_1335 at 1529.943 kb on - strand, within Pf1N1B4_1335 at 1529.943 kb on - strand, within Pf1N1B4_1335 at 1530.017 kb on + strand, within Pf1N1B4_1335 at 1530.018 kb on - strand, within Pf1N1B4_1335 at 1530.195 kb on - strand, within Pf1N1B4_1335 at 1530.195 kb on - strand, within Pf1N1B4_1335 at 1530.201 kb on + strand, within Pf1N1B4_1335 at 1530.201 kb on + strand, within Pf1N1B4_1335 at 1530.201 kb on + strand, within Pf1N1B4_1335 at 1530.201 kb on + strand, within Pf1N1B4_1335 at 1530.201 kb on + strand, within Pf1N1B4_1335 at 1530.202 kb on - strand at 1530.202 kb on - strand, within Pf1N1B4_1335 at 1530.202 kb on - strand, within Pf1N1B4_1335 at 1530.202 kb on - strand, within Pf1N1B4_1335 at 1530.202 kb on - strand, within Pf1N1B4_1335 at 1530.202 kb on - strand, within Pf1N1B4_1335 at 1530.340 kb on + strand at 1530.340 kb on + strand at 1530.340 kb on + strand at 1530.340 kb on + strand at 1530.341 kb on - strand at 1530.537 kb on + strand, within Pf1N1B4_1336 at 1530.537 kb on + strand, within Pf1N1B4_1336 at 1530.538 kb on - strand, within Pf1N1B4_1336 at 1530.538 kb on - strand, within Pf1N1B4_1336 at 1530.824 kb on + strand at 1530.888 kb on - strand at 1531.175 kb on + strand, within Pf1N1B4_1337 at 1531.176 kb on - strand, within Pf1N1B4_1337 at 1531.176 kb on - strand, within Pf1N1B4_1337 at 1531.236 kb on + strand, within Pf1N1B4_1337 at 1531.267 kb on + strand, within Pf1N1B4_1337 at 1531.268 kb on - strand, within Pf1N1B4_1337 at 1531.332 kb on - strand, within Pf1N1B4_1337 at 1531.332 kb on - strand, within Pf1N1B4_1337 at 1531.350 kb on - strand, within Pf1N1B4_1337 at 1531.350 kb on - strand, within Pf1N1B4_1337 at 1531.544 kb on + strand, within Pf1N1B4_1337 at 1531.545 kb on - strand, within Pf1N1B4_1337 at 1531.583 kb on + strand, within Pf1N1B4_1337 at 1531.583 kb on + strand, within Pf1N1B4_1337 at 1531.584 kb on - strand, within Pf1N1B4_1337 at 1531.584 kb on - strand, within Pf1N1B4_1337 at 1531.584 kb on - strand, within Pf1N1B4_1337 at 1531.584 kb on - strand, within Pf1N1B4_1337 at 1531.584 kb on - strand, within Pf1N1B4_1337 at 1531.823 kb on + strand, within Pf1N1B4_1337 at 1531.824 kb on - strand, within Pf1N1B4_1337 at 1531.826 kb on + strand, within Pf1N1B4_1337 at 1531.826 kb on + strand, within Pf1N1B4_1337 at 1531.826 kb on + strand, within Pf1N1B4_1337 at 1531.827 kb on - strand, within Pf1N1B4_1337 at 1531.827 kb on - strand, within Pf1N1B4_1337 at 1531.827 kb on - strand, within Pf1N1B4_1337 at 1531.827 kb on - strand, within Pf1N1B4_1337 at 1531.827 kb on - strand at 1531.827 kb on - strand, within Pf1N1B4_1337 at 1531.827 kb on - strand, within Pf1N1B4_1337 at 1531.827 kb on - strand, within Pf1N1B4_1337 at 1531.827 kb on - strand, within Pf1N1B4_1337 at 1531.827 kb on - strand, within Pf1N1B4_1337 at 1531.827 kb on - strand, within Pf1N1B4_1337 at 1531.832 kb on + strand, within Pf1N1B4_1337 at 1531.832 kb on + strand, within Pf1N1B4_1337 at 1531.832 kb on + strand, within Pf1N1B4_1337 at 1531.833 kb on - strand, within Pf1N1B4_1337 at 1531.833 kb on - strand, within Pf1N1B4_1337 at 1531.833 kb on - strand, within Pf1N1B4_1337 at 1531.833 kb on - strand, within Pf1N1B4_1337 at 1531.856 kb on + strand, within Pf1N1B4_1337 at 1531.856 kb on + strand, within Pf1N1B4_1337 at 1531.856 kb on + strand, within Pf1N1B4_1337 at 1531.856 kb on + strand, within Pf1N1B4_1337 at 1531.856 kb on + strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1531.857 kb on - strand, within Pf1N1B4_1337 at 1532.040 kb on - strand, within Pf1N1B4_1337 at 1532.040 kb on - strand, within Pf1N1B4_1337 at 1532.040 kb on - strand, within Pf1N1B4_1337 at 1532.040 kb on - strand, within Pf1N1B4_1337 at 1532.040 kb on - strand, within Pf1N1B4_1337 at 1532.050 kb on - strand, within Pf1N1B4_1337 at 1532.081 kb on + strand, within Pf1N1B4_1337 at 1532.082 kb on - strand, within Pf1N1B4_1337 at 1532.082 kb on - strand, within Pf1N1B4_1337 at 1532.082 kb on - strand, within Pf1N1B4_1337 at 1532.082 kb on - strand, within Pf1N1B4_1337 at 1532.438 kb on + strand, within Pf1N1B4_1337 at 1532.438 kb on + strand, within Pf1N1B4_1337 at 1532.438 kb on + strand, within Pf1N1B4_1337 at 1532.439 kb on - strand, within Pf1N1B4_1337 at 1532.439 kb on - strand at 1532.439 kb on - strand, within Pf1N1B4_1337 at 1532.439 kb on - strand, within Pf1N1B4_1337 at 1532.474 kb on + strand, within Pf1N1B4_1337 at 1532.474 kb on + strand, within Pf1N1B4_1337 at 1532.474 kb on + strand, within Pf1N1B4_1337 at 1532.475 kb on - strand, within Pf1N1B4_1337 at 1532.475 kb on - strand, within Pf1N1B4_1337 at 1532.475 kb on - strand, within Pf1N1B4_1337 at 1532.495 kb on + strand, within Pf1N1B4_1337 at 1532.495 kb on + strand, within Pf1N1B4_1337 at 1532.495 kb on + strand, within Pf1N1B4_1337 at 1532.496 kb on - strand, within Pf1N1B4_1337 at 1532.496 kb on - strand, within Pf1N1B4_1337 at 1532.496 kb on - strand, within Pf1N1B4_1337 at 1532.496 kb on - strand, within Pf1N1B4_1337 at 1532.510 kb on + strand, within Pf1N1B4_1337 at 1532.510 kb on + strand, within Pf1N1B4_1337 at 1532.510 kb on + strand, within Pf1N1B4_1337 at 1532.591 kb on + strand, within Pf1N1B4_1337 at 1532.592 kb on - strand, within Pf1N1B4_1337 at 1532.592 kb on - strand at 1532.592 kb on - strand, within Pf1N1B4_1337 at 1532.592 kb on - strand, within Pf1N1B4_1337 at 1532.592 kb on - strand, within Pf1N1B4_1337 at 1532.634 kb on - strand, within Pf1N1B4_1337 at 1532.711 kb on + strand, within Pf1N1B4_1337 at 1532.711 kb on + strand, within Pf1N1B4_1337 at 1532.711 kb on + strand, within Pf1N1B4_1337 at 1532.712 kb on - strand, within Pf1N1B4_1337 at 1532.913 kb on - strand at 1533.016 kb on + strand at 1533.017 kb on - strand at 1533.017 kb on - strand at 1533.017 kb on - strand at 1533.027 kb on - strand at 1533.184 kb on + strand at 1533.184 kb on + strand at 1533.185 kb on - strand at 1533.185 kb on - strand at 1533.195 kb on + strand at 1533.195 kb on + strand at 1533.195 kb on + strand at 1533.195 kb on + strand at 1533.195 kb on + strand at 1533.196 kb on - strand at 1533.249 kb on + strand at 1533.276 kb on + strand at 1533.277 kb on - strand at 1533.277 kb on - strand at 1533.277 kb on - strand at 1533.277 kb on - strand at 1533.277 kb on - strand at 1533.334 kb on - strand at 1533.334 kb on - strand at 1533.334 kb on - strand at 1533.334 kb on - strand at 1533.334 kb on - strand at 1533.334 kb on - strand at 1533.366 kb on + strand, within Pf1N1B4_1338 at 1533.366 kb on + strand, within Pf1N1B4_1338 at 1533.468 kb on + strand, within Pf1N1B4_1338 at 1533.469 kb on - strand, within Pf1N1B4_1338 at 1533.469 kb on - strand, within Pf1N1B4_1338 at 1533.469 kb on - strand, within Pf1N1B4_1338 at 1533.469 kb on - strand, within Pf1N1B4_1338 at 1533.553 kb on - strand, within Pf1N1B4_1338 at 1533.658 kb on + strand, within Pf1N1B4_1338 at 1533.658 kb on + strand, within Pf1N1B4_1338 at 1533.659 kb on - strand, within Pf1N1B4_1338 at 1533.659 kb on - strand, within Pf1N1B4_1338 at 1533.659 kb on - strand, within Pf1N1B4_1338 at 1533.659 kb on - strand, within Pf1N1B4_1338 at 1533.876 kb on + strand, within Pf1N1B4_1338 at 1533.876 kb on + strand, within Pf1N1B4_1338 at 1533.876 kb on + strand, within Pf1N1B4_1338
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 1,529,942 + Pf1N1B4_1335 0.14 -0.2 1,529,942 + Pf1N1B4_1335 0.14 -2.2 1,529,942 + Pf1N1B4_1335 0.14 +1.6 1,529,942 + Pf1N1B4_1335 0.14 -0.1 1,529,942 + Pf1N1B4_1335 0.14 +0.2 1,529,943 - Pf1N1B4_1335 0.14 +3.6 1,529,943 - Pf1N1B4_1335 0.14 +3.8 1,529,943 - Pf1N1B4_1335 0.14 +2.7 1,530,017 + Pf1N1B4_1335 0.29 +0.6 1,530,018 - Pf1N1B4_1335 0.30 +3.5 1,530,195 - Pf1N1B4_1335 0.66 +4.1 1,530,195 - Pf1N1B4_1335 0.66 +2.9 1,530,201 + Pf1N1B4_1335 0.68 +0.9 1,530,201 + Pf1N1B4_1335 0.68 -0.4 1,530,201 + Pf1N1B4_1335 0.68 +0.3 1,530,201 + Pf1N1B4_1335 0.68 +0.9 1,530,201 + Pf1N1B4_1335 0.68 +1.5 1,530,202 - +3.7 1,530,202 - Pf1N1B4_1335 0.68 +2.1 1,530,202 - Pf1N1B4_1335 0.68 +3.5 1,530,202 - Pf1N1B4_1335 0.68 +3.3 1,530,202 - Pf1N1B4_1335 0.68 +3.9 1,530,202 - Pf1N1B4_1335 0.68 +3.4 1,530,340 + -1.3 1,530,340 + -0.3 1,530,340 + -0.3 1,530,340 + -0.7 1,530,341 - -2.0 1,530,537 + Pf1N1B4_1336 0.28 +1.8 1,530,537 + Pf1N1B4_1336 0.28 +2.7 1,530,538 - Pf1N1B4_1336 0.29 +1.4 1,530,538 - Pf1N1B4_1336 0.29 +1.6 1,530,824 + -1.6 1,530,888 - +3.2 1,531,175 + Pf1N1B4_1337 0.12 +2.1 1,531,176 - Pf1N1B4_1337 0.13 +2.2 1,531,176 - Pf1N1B4_1337 0.13 +2.8 1,531,236 + Pf1N1B4_1337 0.15 +0.4 1,531,267 + Pf1N1B4_1337 0.17 -1.7 1,531,268 - Pf1N1B4_1337 0.17 +2.5 1,531,332 - Pf1N1B4_1337 0.20 +2.9 1,531,332 - Pf1N1B4_1337 0.20 +3.2 1,531,350 - Pf1N1B4_1337 0.21 +2.4 1,531,350 - Pf1N1B4_1337 0.21 +2.6 1,531,544 + Pf1N1B4_1337 0.30 +0.7 1,531,545 - Pf1N1B4_1337 0.30 +2.1 1,531,583 + Pf1N1B4_1337 0.32 -0.7 1,531,583 + Pf1N1B4_1337 0.32 +1.8 1,531,584 - Pf1N1B4_1337 0.32 +1.1 1,531,584 - Pf1N1B4_1337 0.32 +2.8 1,531,584 - Pf1N1B4_1337 0.32 +2.7 1,531,584 - Pf1N1B4_1337 0.32 +2.6 1,531,584 - Pf1N1B4_1337 0.32 +1.8 1,531,823 + Pf1N1B4_1337 0.43 +1.8 1,531,824 - Pf1N1B4_1337 0.43 +2.4 1,531,826 + Pf1N1B4_1337 0.43 -0.7 1,531,826 + Pf1N1B4_1337 0.43 -1.8 1,531,826 + Pf1N1B4_1337 0.43 +1.5 1,531,827 - Pf1N1B4_1337 0.43 +2.6 1,531,827 - Pf1N1B4_1337 0.43 +3.3 1,531,827 - Pf1N1B4_1337 0.43 +2.3 1,531,827 - Pf1N1B4_1337 0.43 +2.4 1,531,827 - +1.6 1,531,827 - Pf1N1B4_1337 0.43 +1.1 1,531,827 - Pf1N1B4_1337 0.43 +2.9 1,531,827 - Pf1N1B4_1337 0.43 +1.0 1,531,827 - Pf1N1B4_1337 0.43 +2.3 1,531,827 - Pf1N1B4_1337 0.43 +2.8 1,531,827 - Pf1N1B4_1337 0.43 +2.0 1,531,832 + Pf1N1B4_1337 0.43 -1.8 1,531,832 + Pf1N1B4_1337 0.43 -0.8 1,531,832 + Pf1N1B4_1337 0.43 +1.2 1,531,833 - Pf1N1B4_1337 0.44 +2.3 1,531,833 - Pf1N1B4_1337 0.44 +2.8 1,531,833 - Pf1N1B4_1337 0.44 +3.1 1,531,833 - Pf1N1B4_1337 0.44 +3.5 1,531,856 + Pf1N1B4_1337 0.45 +1.8 1,531,856 + Pf1N1B4_1337 0.45 -0.4 1,531,856 + Pf1N1B4_1337 0.45 +0.7 1,531,856 + Pf1N1B4_1337 0.45 +0.8 1,531,856 + Pf1N1B4_1337 0.45 +2.2 1,531,857 - Pf1N1B4_1337 0.45 -0.1 1,531,857 - Pf1N1B4_1337 0.45 +3.1 1,531,857 - Pf1N1B4_1337 0.45 +3.0 1,531,857 - Pf1N1B4_1337 0.45 +1.7 1,531,857 - Pf1N1B4_1337 0.45 +2.3 1,531,857 - Pf1N1B4_1337 0.45 +3.7 1,531,857 - Pf1N1B4_1337 0.45 +2.1 1,531,857 - Pf1N1B4_1337 0.45 +2.9 1,531,857 - Pf1N1B4_1337 0.45 +2.8 1,531,857 - Pf1N1B4_1337 0.45 +2.3 1,531,857 - Pf1N1B4_1337 0.45 -1.7 1,531,857 - Pf1N1B4_1337 0.45 +1.6 1,531,857 - Pf1N1B4_1337 0.45 +1.7 1,532,040 - Pf1N1B4_1337 0.53 +2.9 1,532,040 - Pf1N1B4_1337 0.53 +2.5 1,532,040 - Pf1N1B4_1337 0.53 +2.1 1,532,040 - Pf1N1B4_1337 0.53 +2.4 1,532,040 - Pf1N1B4_1337 0.53 +1.5 1,532,050 - Pf1N1B4_1337 0.54 +2.8 1,532,081 + Pf1N1B4_1337 0.55 -1.7 1,532,082 - Pf1N1B4_1337 0.55 +0.5 1,532,082 - Pf1N1B4_1337 0.55 +2.5 1,532,082 - Pf1N1B4_1337 0.55 +1.7 1,532,082 - Pf1N1B4_1337 0.55 +2.2 1,532,438 + Pf1N1B4_1337 0.72 +1.0 1,532,438 + Pf1N1B4_1337 0.72 -0.6 1,532,438 + Pf1N1B4_1337 0.72 +1.3 1,532,439 - Pf1N1B4_1337 0.72 +2.9 1,532,439 - +2.3 1,532,439 - Pf1N1B4_1337 0.72 +2.2 1,532,439 - Pf1N1B4_1337 0.72 +2.9 1,532,474 + Pf1N1B4_1337 0.74 -1.4 1,532,474 + Pf1N1B4_1337 0.74 -0.1 1,532,474 + Pf1N1B4_1337 0.74 +0.9 1,532,475 - Pf1N1B4_1337 0.74 +3.7 1,532,475 - Pf1N1B4_1337 0.74 +2.9 1,532,475 - Pf1N1B4_1337 0.74 +2.6 1,532,495 + Pf1N1B4_1337 0.75 -1.1 1,532,495 + Pf1N1B4_1337 0.75 -0.4 1,532,495 + Pf1N1B4_1337 0.75 -0.2 1,532,496 - Pf1N1B4_1337 0.75 +1.1 1,532,496 - Pf1N1B4_1337 0.75 -0.1 1,532,496 - Pf1N1B4_1337 0.75 +1.9 1,532,496 - Pf1N1B4_1337 0.75 +3.4 1,532,510 + Pf1N1B4_1337 0.75 -0.0 1,532,510 + Pf1N1B4_1337 0.75 +0.5 1,532,510 + Pf1N1B4_1337 0.75 +2.1 1,532,591 + Pf1N1B4_1337 0.79 +0.2 1,532,592 - Pf1N1B4_1337 0.79 +2.3 1,532,592 - +3.3 1,532,592 - Pf1N1B4_1337 0.79 +3.1 1,532,592 - Pf1N1B4_1337 0.79 +0.8 1,532,592 - Pf1N1B4_1337 0.79 +1.1 1,532,634 - Pf1N1B4_1337 0.81 +2.2 1,532,711 + Pf1N1B4_1337 0.85 -1.6 1,532,711 + Pf1N1B4_1337 0.85 +1.4 1,532,711 + Pf1N1B4_1337 0.85 +0.6 1,532,712 - Pf1N1B4_1337 0.85 +0.6 1,532,913 - +0.9 1,533,016 + -0.6 1,533,017 - +2.0 1,533,017 - +2.0 1,533,017 - +2.0 1,533,027 - +3.2 1,533,184 + -1.3 1,533,184 + -1.7 1,533,185 - +1.2 1,533,185 - -0.8 1,533,195 + -2.6 1,533,195 + -1.1 1,533,195 + -1.1 1,533,195 + -0.3 1,533,195 + +0.9 1,533,196 - -0.7 1,533,249 + +0.4 1,533,276 + +0.6 1,533,277 - -0.5 1,533,277 - +0.8 1,533,277 - +0.6 1,533,277 - -2.5 1,533,277 - +0.0 1,533,334 - +1.8 1,533,334 - +0.8 1,533,334 - +0.4 1,533,334 - -0.2 1,533,334 - -0.6 1,533,334 - +0.6 1,533,366 + Pf1N1B4_1338 0.10 -2.2 1,533,366 + Pf1N1B4_1338 0.10 -0.5 1,533,468 + Pf1N1B4_1338 0.18 +1.7 1,533,469 - Pf1N1B4_1338 0.18 -0.7 1,533,469 - Pf1N1B4_1338 0.18 +1.3 1,533,469 - Pf1N1B4_1338 0.18 +0.4 1,533,469 - Pf1N1B4_1338 0.18 +1.4 1,533,553 - Pf1N1B4_1338 0.24 -0.4 1,533,658 + Pf1N1B4_1338 0.31 +0.5 1,533,658 + Pf1N1B4_1338 0.31 -1.0 1,533,659 - Pf1N1B4_1338 0.32 -1.1 1,533,659 - Pf1N1B4_1338 0.32 +1.8 1,533,659 - Pf1N1B4_1338 0.32 +0.6 1,533,659 - Pf1N1B4_1338 0.32 -1.2 1,533,876 + Pf1N1B4_1338 0.47 +2.8 1,533,876 + Pf1N1B4_1338 0.47 -0.6 1,533,876 + Pf1N1B4_1338 0.47 +0.8
Or see this region's nucleotide sequence