Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1046 and Pf1N1B4_1047 overlap by 18 nucleotides Pf1N1B4_1047 and Pf1N1B4_1048 are separated by 198 nucleotides Pf1N1B4_1048 and Pf1N1B4_1049 are separated by 64 nucleotides
Pf1N1B4_1046 - Thioredoxin reductase (EC 1.8.1.9), at 1,182,833 to 1,183,789
_1046
Pf1N1B4_1047 - hypothetical protein, at 1,183,772 to 1,183,906
_1047
Pf1N1B4_1048 - Sulfate transporter, CysZ-type, at 1,184,105 to 1,184,860
_1048
Pf1N1B4_1049 - Glycosyl transferase, group 1 family protein, at 1,184,925 to 1,186,100
_1049
Position (kb)
1184
1185 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1183.904 kb on + strand at 1183.904 kb on + strand at 1183.904 kb on + strand at 1183.904 kb on + strand at 1183.971 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1184.000 kb on - strand at 1184.000 kb on - strand at 1184.000 kb on - strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.002 kb on - strand at 1184.002 kb on - strand at 1184.002 kb on - strand at 1184.002 kb on - strand at 1184.002 kb on - strand at 1184.003 kb on + strand at 1184.003 kb on + strand at 1184.003 kb on + strand at 1184.003 kb on + strand at 1184.003 kb on + strand at 1184.003 kb on + strand at 1184.004 kb on - strand at 1184.016 kb on + strand at 1184.016 kb on + strand at 1184.016 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.135 kb on + strand at 1184.191 kb on - strand, within Pf1N1B4_1048 at 1184.234 kb on + strand, within Pf1N1B4_1048 at 1184.234 kb on + strand, within Pf1N1B4_1048 at 1184.234 kb on + strand, within Pf1N1B4_1048 at 1184.234 kb on + strand, within Pf1N1B4_1048 at 1184.235 kb on - strand, within Pf1N1B4_1048 at 1184.235 kb on - strand at 1184.257 kb on + strand, within Pf1N1B4_1048 at 1184.257 kb on + strand, within Pf1N1B4_1048 at 1184.257 kb on + strand, within Pf1N1B4_1048 at 1184.258 kb on - strand, within Pf1N1B4_1048 at 1184.258 kb on - strand, within Pf1N1B4_1048 at 1184.258 kb on - strand, within Pf1N1B4_1048 at 1184.312 kb on + strand, within Pf1N1B4_1048 at 1184.312 kb on + strand, within Pf1N1B4_1048 at 1184.313 kb on - strand, within Pf1N1B4_1048 at 1184.313 kb on - strand, within Pf1N1B4_1048 at 1184.313 kb on - strand, within Pf1N1B4_1048 at 1184.942 kb on + strand at 1184.942 kb on + strand at 1184.942 kb on + strand at 1185.078 kb on - strand, within Pf1N1B4_1049 at 1185.207 kb on + strand, within Pf1N1B4_1049 at 1185.208 kb on - strand, within Pf1N1B4_1049 at 1185.208 kb on - strand, within Pf1N1B4_1049 at 1185.306 kb on + strand, within Pf1N1B4_1049 at 1185.306 kb on + strand, within Pf1N1B4_1049 at 1185.306 kb on + strand, within Pf1N1B4_1049 at 1185.306 kb on + strand at 1185.307 kb on - strand, within Pf1N1B4_1049 at 1185.307 kb on - strand, within Pf1N1B4_1049 at 1185.354 kb on + strand, within Pf1N1B4_1049 at 1185.354 kb on + strand, within Pf1N1B4_1049 at 1185.540 kb on - strand, within Pf1N1B4_1049 at 1185.540 kb on - strand, within Pf1N1B4_1049 at 1185.557 kb on - strand, within Pf1N1B4_1049 at 1185.557 kb on - strand, within Pf1N1B4_1049 at 1185.625 kb on - strand, within Pf1N1B4_1049 at 1185.625 kb on - strand, within Pf1N1B4_1049 at 1185.748 kb on - strand, within Pf1N1B4_1049 at 1185.843 kb on + strand, within Pf1N1B4_1049
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 1,183,904 + +3.2 1,183,904 + +3.2 1,183,904 + +3.6 1,183,904 + +1.9 1,183,971 + +3.3 1,183,999 + +0.8 1,183,999 + +1.4 1,183,999 + +0.4 1,183,999 + +2.6 1,183,999 + +1.7 1,183,999 + -0.1 1,183,999 + +1.8 1,183,999 + +0.9 1,183,999 + -1.5 1,183,999 + +1.6 1,184,000 - -0.2 1,184,000 - -1.6 1,184,000 - +1.3 1,184,001 + +2.1 1,184,001 + +1.1 1,184,001 + +0.3 1,184,001 + +1.7 1,184,001 + +1.2 1,184,001 + +1.1 1,184,001 + +0.5 1,184,001 + +1.4 1,184,001 + -0.8 1,184,001 + +0.3 1,184,002 - -1.9 1,184,002 - -1.1 1,184,002 - +0.9 1,184,002 - +2.0 1,184,002 - +1.0 1,184,003 + +2.3 1,184,003 + +1.0 1,184,003 + +1.6 1,184,003 + +1.8 1,184,003 + +2.9 1,184,003 + +2.0 1,184,004 - +0.5 1,184,016 + +0.6 1,184,016 + +3.0 1,184,016 + +1.1 1,184,022 + +2.1 1,184,022 + +1.5 1,184,022 + +2.5 1,184,022 + +1.8 1,184,022 + +0.3 1,184,022 + +0.4 1,184,022 + +2.1 1,184,022 + +1.6 1,184,022 + +2.4 1,184,135 + -0.7 1,184,191 - Pf1N1B4_1048 0.11 -2.2 1,184,234 + Pf1N1B4_1048 0.17 +2.4 1,184,234 + Pf1N1B4_1048 0.17 -2.5 1,184,234 + Pf1N1B4_1048 0.17 +0.0 1,184,234 + Pf1N1B4_1048 0.17 -1.2 1,184,235 - Pf1N1B4_1048 0.17 -0.4 1,184,235 - +1.2 1,184,257 + Pf1N1B4_1048 0.20 -1.5 1,184,257 + Pf1N1B4_1048 0.20 -0.2 1,184,257 + Pf1N1B4_1048 0.20 -0.2 1,184,258 - Pf1N1B4_1048 0.20 -1.3 1,184,258 - Pf1N1B4_1048 0.20 -1.2 1,184,258 - Pf1N1B4_1048 0.20 -1.7 1,184,312 + Pf1N1B4_1048 0.27 -1.8 1,184,312 + Pf1N1B4_1048 0.27 -1.4 1,184,313 - Pf1N1B4_1048 0.28 -0.2 1,184,313 - Pf1N1B4_1048 0.28 -3.0 1,184,313 - Pf1N1B4_1048 0.28 -2.0 1,184,942 + +1.2 1,184,942 + -2.1 1,184,942 + +1.8 1,185,078 - Pf1N1B4_1049 0.13 +1.4 1,185,207 + Pf1N1B4_1049 0.24 +1.7 1,185,208 - Pf1N1B4_1049 0.24 -2.2 1,185,208 - Pf1N1B4_1049 0.24 +1.2 1,185,306 + Pf1N1B4_1049 0.32 +0.4 1,185,306 + Pf1N1B4_1049 0.32 -0.5 1,185,306 + Pf1N1B4_1049 0.32 -3.1 1,185,306 + -1.5 1,185,307 - Pf1N1B4_1049 0.32 -0.6 1,185,307 - Pf1N1B4_1049 0.32 -2.4 1,185,354 + Pf1N1B4_1049 0.36 -2.3 1,185,354 + Pf1N1B4_1049 0.36 +0.8 1,185,540 - Pf1N1B4_1049 0.52 -0.5 1,185,540 - Pf1N1B4_1049 0.52 -0.1 1,185,557 - Pf1N1B4_1049 0.54 +0.2 1,185,557 - Pf1N1B4_1049 0.54 +1.1 1,185,625 - Pf1N1B4_1049 0.60 +1.0 1,185,625 - Pf1N1B4_1049 0.60 +1.0 1,185,748 - Pf1N1B4_1049 0.70 +1.6 1,185,843 + Pf1N1B4_1049 0.78 -0.9
Or see this region's nucleotide sequence