Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03025 and MMP_RS03030 are separated by 541 nucleotides MMP_RS03030 and MMP_RS03035 are separated by 17 nucleotides MMP_RS03035 and MMP_RS03040 are separated by 9 nucleotides MMP_RS03040 and MMP_RS03045 are separated by 79 nucleotides MMP_RS03045 and MMP_RS03050 are separated by 99 nucleotides
MMP_RS03025: MMP_RS03025 - molybdenum cofactor guanylyltransferase, at 572,518 to 573,162
_RS03025
MMP_RS03030: MMP_RS03030 - Asp-tRNA(Asn) amidotransferase subunit GatC, at 573,704 to 573,943
_RS03030
MMP_RS03035: MMP_RS03035 - 4-hydroxy-tetrahydrodipicolinate synthase, at 573,961 to 574,830
_RS03035
MMP_RS03040: MMP_RS03040 - 30S ribosomal protein S17e, at 574,840 to 575,031
_RS03040
MMP_RS03045: MMP_RS03045 - chorismate mutase, at 575,111 to 575,401
_RS03045
MMP_RS03050: MMP_RS03050 - DUF115 domain-containing protein, at 575,501 to 576,217
_RS03050
Position (kb) 573
574
575 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 572.965 kb on - strand, within MMP_RS03025 at 573.107 kb on - strand at 573.107 kb on - strand at 573.107 kb on - strand at 573.111 kb on - strand at 573.128 kb on - strand at 573.172 kb on - strand at 573.193 kb on + strand at 573.193 kb on + strand at 573.200 kb on + strand at 573.200 kb on + strand at 573.200 kb on + strand at 573.228 kb on - strand at 573.260 kb on - strand at 573.290 kb on - strand at 573.309 kb on - strand at 573.310 kb on + strand at 573.310 kb on + strand at 573.350 kb on + strand at 573.354 kb on - strand at 573.381 kb on - strand at 573.396 kb on - strand at 573.396 kb on - strand at 573.396 kb on - strand at 573.424 kb on - strand at 573.425 kb on + strand at 573.425 kb on + strand at 573.425 kb on + strand at 573.426 kb on - strand at 573.426 kb on - strand at 573.427 kb on + strand at 573.428 kb on - strand at 573.429 kb on + strand at 573.526 kb on - strand at 573.527 kb on + strand at 573.562 kb on + strand at 573.565 kb on - strand at 573.565 kb on - strand at 573.565 kb on - strand at 573.566 kb on + strand at 573.566 kb on + strand at 573.604 kb on + strand at 573.754 kb on - strand at 573.943 kb on - strand at 574.069 kb on + strand, within MMP_RS03035 at 574.291 kb on - strand, within MMP_RS03035 at 574.365 kb on - strand, within MMP_RS03035 at 574.440 kb on + strand, within MMP_RS03035 at 574.441 kb on - strand, within MMP_RS03035 at 574.447 kb on - strand, within MMP_RS03035 at 574.447 kb on - strand, within MMP_RS03035 at 574.518 kb on + strand, within MMP_RS03035 at 575.050 kb on - strand at 575.094 kb on - strand at 575.096 kb on - strand at 575.192 kb on - strand at 575.333 kb on - strand at 575.402 kb on + strand at 575.402 kb on + strand at 575.451 kb on - strand at 575.490 kb on + strand at 575.644 kb on + strand at 575.737 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 572,965 - MMP_RS03025 0.69 -1.0 573,107 - -2.2 573,107 - -1.4 573,107 - -1.6 573,111 - -0.4 573,128 - -2.0 573,172 - -0.4 573,193 + +0.0 573,193 + -0.2 573,200 + -0.0 573,200 + +0.1 573,200 + -0.1 573,228 - +0.4 573,260 - +0.0 573,290 - -0.1 573,309 - +0.6 573,310 + -0.4 573,310 + +0.1 573,350 + -0.0 573,354 - -0.6 573,381 - +0.4 573,396 - -0.1 573,396 - -0.1 573,396 - -0.3 573,424 - +0.0 573,425 + -0.2 573,425 + -0.6 573,425 + +0.2 573,426 - +0.2 573,426 - +0.4 573,427 + +0.1 573,428 - +0.2 573,429 + +0.3 573,526 - -0.1 573,527 + -0.1 573,562 + +0.1 573,565 - -0.5 573,565 - -0.7 573,565 - -0.8 573,566 + +0.1 573,566 + +0.1 573,604 + -1.9 573,754 - -0.4 573,943 - -2.3 574,069 + MMP_RS03035 0.12 -3.8 574,291 - MMP_RS03035 0.38 +0.2 574,365 - MMP_RS03035 0.46 -3.5 574,440 + MMP_RS03035 0.55 -1.0 574,441 - MMP_RS03035 0.55 -1.5 574,447 - MMP_RS03035 0.56 -0.7 574,447 - MMP_RS03035 0.56 -0.5 574,518 + MMP_RS03035 0.64 -2.0 575,050 - -0.3 575,094 - -1.0 575,096 - -0.9 575,192 - -0.0 575,333 - -1.0 575,402 + -0.8 575,402 + -2.1 575,451 - -1.7 575,490 + -0.0 575,644 + -0.2 575,737 + -0.7
Or see this region's nucleotide sequence