Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02230 and MMP_RS08940 are separated by 33 nucleotides MMP_RS08940 and MMP_RS02235 are separated by 195 nucleotides MMP_RS02235 and MMP_RS02240 are separated by 17 nucleotides MMP_RS02240 and MMP_RS02245 are separated by 30 nucleotides
MMP_RS02230: MMP_RS02230 - sodium-dependent transporter, at 420,466 to 421,977
_RS02230
MMP_RS08940: MMP_RS08940 - MetS family NSS transporter small subunit, at 422,011 to 422,091
_RS08940
MMP_RS02235: MMP_RS02235 - CBS domain-containing protein, at 422,287 to 423,252
_RS02235
MMP_RS02240: MMP_RS02240 - methanogenesis marker 7 protein, at 423,270 to 424,184
_RS02240
MMP_RS02245: MMP_RS02245 - hypothetical protein, at 424,215 to 424,667
_RS02245
Position (kb) 422
423
424 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 421.326 kb on - strand, within MMP_RS02230 at 421.359 kb on + strand, within MMP_RS02230 at 421.409 kb on - strand, within MMP_RS02230 at 421.410 kb on + strand, within MMP_RS02230 at 421.410 kb on + strand, within MMP_RS02230 at 421.422 kb on + strand, within MMP_RS02230 at 421.615 kb on - strand, within MMP_RS02230 at 421.784 kb on + strand, within MMP_RS02230 at 421.983 kb on - strand at 422.009 kb on - strand at 422.010 kb on + strand at 422.036 kb on - strand, within MMP_RS08940 at 422.089 kb on - strand at 422.090 kb on + strand at 422.092 kb on - strand at 422.093 kb on + strand at 422.160 kb on - strand at 422.163 kb on + strand at 422.181 kb on - strand at 422.227 kb on - strand at 422.232 kb on - strand at 422.232 kb on - strand at 422.232 kb on - strand at 422.232 kb on - strand at 422.232 kb on - strand at 422.233 kb on + strand at 422.233 kb on + strand at 422.234 kb on - strand at 422.235 kb on + strand at 422.241 kb on - strand at 422.291 kb on - strand at 422.292 kb on + strand at 422.321 kb on - strand at 422.323 kb on - strand at 422.323 kb on - strand at 422.324 kb on + strand at 422.324 kb on + strand at 422.325 kb on - strand at 422.325 kb on - strand at 422.325 kb on - strand at 422.326 kb on + strand at 422.326 kb on + strand at 422.326 kb on + strand at 422.352 kb on - strand at 422.356 kb on - strand at 422.356 kb on - strand at 422.357 kb on + strand at 422.357 kb on + strand at 422.360 kb on - strand at 422.368 kb on - strand at 422.368 kb on - strand at 422.372 kb on - strand at 422.376 kb on - strand at 422.376 kb on - strand at 422.456 kb on - strand, within MMP_RS02235 at 422.456 kb on - strand, within MMP_RS02235 at 422.457 kb on + strand, within MMP_RS02235 at 422.471 kb on - strand, within MMP_RS02235 at 422.472 kb on + strand, within MMP_RS02235 at 422.518 kb on + strand, within MMP_RS02235 at 422.554 kb on + strand, within MMP_RS02235 at 422.623 kb on + strand, within MMP_RS02235 at 422.651 kb on - strand, within MMP_RS02235 at 422.794 kb on + strand, within MMP_RS02235 at 422.829 kb on - strand, within MMP_RS02235 at 422.863 kb on - strand, within MMP_RS02235 at 422.938 kb on - strand, within MMP_RS02235 at 422.938 kb on - strand, within MMP_RS02235 at 423.055 kb on + strand, within MMP_RS02235 at 423.071 kb on - strand, within MMP_RS02235 at 423.076 kb on + strand, within MMP_RS02235 at 423.090 kb on + strand, within MMP_RS02235 at 423.126 kb on + strand, within MMP_RS02235 at 423.126 kb on + strand, within MMP_RS02235 at 423.191 kb on - strand at 423.282 kb on + strand at 423.282 kb on + strand at 423.284 kb on - strand at 423.285 kb on + strand at 423.445 kb on + strand, within MMP_RS02240 at 423.777 kb on - strand, within MMP_RS02240 at 423.885 kb on + strand, within MMP_RS02240 at 423.959 kb on + strand, within MMP_RS02240 at 423.959 kb on + strand, within MMP_RS02240 at 424.077 kb on - strand, within MMP_RS02240 at 424.098 kb on - strand at 424.099 kb on + strand at 424.104 kb on + strand at 424.183 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 421,326 - MMP_RS02230 0.57 -0.4 421,359 + MMP_RS02230 0.59 -0.7 421,409 - MMP_RS02230 0.62 -0.5 421,410 + MMP_RS02230 0.62 -1.1 421,410 + MMP_RS02230 0.62 -0.2 421,422 + MMP_RS02230 0.63 +0.2 421,615 - MMP_RS02230 0.76 -0.0 421,784 + MMP_RS02230 0.87 -0.2 421,983 - -0.0 422,009 - -0.4 422,010 + -0.3 422,036 - MMP_RS08940 0.31 +0.1 422,089 - +0.8 422,090 + +0.1 422,092 - +0.7 422,093 + +0.2 422,160 - -0.6 422,163 + -0.4 422,181 - +0.4 422,227 - -0.8 422,232 - -1.3 422,232 - -0.5 422,232 - -0.2 422,232 - +0.1 422,232 - -0.6 422,233 + +0.1 422,233 + -0.6 422,234 - -0.3 422,235 + -0.4 422,241 - -0.7 422,291 - -1.3 422,292 + -0.9 422,321 - -0.6 422,323 - -0.9 422,323 - -0.7 422,324 + +0.4 422,324 + -0.6 422,325 - -0.8 422,325 - -1.1 422,325 - +0.0 422,326 + -0.5 422,326 + -0.7 422,326 + -0.1 422,352 - -0.5 422,356 - -0.9 422,356 - -0.7 422,357 + -0.1 422,357 + -0.0 422,360 - -0.8 422,368 - -0.9 422,368 - -0.5 422,372 - -1.0 422,376 - -0.6 422,376 - +0.0 422,456 - MMP_RS02235 0.17 -0.6 422,456 - MMP_RS02235 0.17 -0.3 422,457 + MMP_RS02235 0.18 -1.4 422,471 - MMP_RS02235 0.19 -0.8 422,472 + MMP_RS02235 0.19 -0.8 422,518 + MMP_RS02235 0.24 -0.9 422,554 + MMP_RS02235 0.28 -0.7 422,623 + MMP_RS02235 0.35 -1.2 422,651 - MMP_RS02235 0.38 -0.8 422,794 + MMP_RS02235 0.52 -0.3 422,829 - MMP_RS02235 0.56 -1.0 422,863 - MMP_RS02235 0.60 -0.7 422,938 - MMP_RS02235 0.67 -0.9 422,938 - MMP_RS02235 0.67 -1.2 423,055 + MMP_RS02235 0.80 -1.3 423,071 - MMP_RS02235 0.81 -1.1 423,076 + MMP_RS02235 0.82 -0.5 423,090 + MMP_RS02235 0.83 -0.9 423,126 + MMP_RS02235 0.87 -0.3 423,126 + MMP_RS02235 0.87 -1.4 423,191 - -1.7 423,282 + -1.7 423,282 + -1.2 423,284 - -0.3 423,285 + -1.1 423,445 + MMP_RS02240 0.19 -1.6 423,777 - MMP_RS02240 0.55 -0.2 423,885 + MMP_RS02240 0.67 -0.7 423,959 + MMP_RS02240 0.75 -3.1 423,959 + MMP_RS02240 0.75 -1.6 424,077 - MMP_RS02240 0.88 -1.7 424,098 - -1.3 424,099 + -2.0 424,104 + -1.1 424,183 + -0.2
Or see this region's nucleotide sequence