Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00730 and MMP_RS00735 are separated by 59 nucleotides MMP_RS00735 and MMP_RS00740 are separated by 306 nucleotides MMP_RS00740 and MMP_RS00745 are separated by 77 nucleotides
MMP_RS00730: MMP_RS00730 - 5 10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB, at 132,540 to 133,565
_RS00730
MMP_RS00735: MMP_RS00735 - 5 10-methenyltetrahydromethanopterin hydrogenase, at 133,625 to 134,689
_RS00735
MMP_RS00740: MMP_RS00740 - cyclase family protein, at 134,996 to 135,565
_RS00740
MMP_RS00745: MMP_RS00745 - histidinol phosphate phosphatase domain-containing protein, at 135,643 to 136,299
_RS00745
Position (kb) 133
134
135 Strain fitness (log2 ratio) -2
-1
0
1 at 132.899 kb on - strand, within MMP_RS00730 at 132.942 kb on - strand, within MMP_RS00730 at 133.070 kb on - strand, within MMP_RS00730 at 133.104 kb on - strand, within MMP_RS00730 at 133.174 kb on + strand, within MMP_RS00730 at 133.288 kb on + strand, within MMP_RS00730 at 133.362 kb on - strand, within MMP_RS00730 at 133.441 kb on - strand, within MMP_RS00730 at 133.490 kb on - strand at 133.586 kb on - strand at 133.609 kb on - strand at 133.622 kb on - strand at 133.713 kb on + strand at 134.255 kb on - strand, within MMP_RS00735 at 134.255 kb on - strand, within MMP_RS00735 at 134.424 kb on + strand, within MMP_RS00735 at 134.713 kb on - strand at 134.713 kb on - strand at 134.723 kb on - strand at 134.757 kb on + strand at 134.760 kb on - strand at 134.760 kb on - strand at 134.782 kb on + strand at 134.863 kb on - strand at 134.900 kb on + strand at 134.982 kb on + strand at 135.287 kb on - strand, within MMP_RS00740 at 135.338 kb on + strand, within MMP_RS00740 at 135.421 kb on - strand, within MMP_RS00740 at 135.422 kb on + strand, within MMP_RS00740 at 135.565 kb on - strand at 135.600 kb on + strand at 135.606 kb on + strand at 135.613 kb on - strand at 135.613 kb on - strand at 135.619 kb on + strand at 135.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 132,899 - MMP_RS00730 0.35 -0.6 132,942 - MMP_RS00730 0.39 -0.1 133,070 - MMP_RS00730 0.52 -0.6 133,104 - MMP_RS00730 0.55 -0.3 133,174 + MMP_RS00730 0.62 -0.7 133,288 + MMP_RS00730 0.73 -0.7 133,362 - MMP_RS00730 0.80 -0.7 133,441 - MMP_RS00730 0.88 -1.1 133,490 - -0.3 133,586 - -0.9 133,609 - -0.4 133,622 - -1.0 133,713 + -1.4 134,255 - MMP_RS00735 0.59 -0.9 134,255 - MMP_RS00735 0.59 -1.0 134,424 + MMP_RS00735 0.75 -1.3 134,713 - -1.4 134,713 - -0.7 134,723 - -1.2 134,757 + -1.8 134,760 - -0.9 134,760 - +0.3 134,782 + +0.4 134,863 - -0.4 134,900 + -0.2 134,982 + -0.2 135,287 - MMP_RS00740 0.51 +0.5 135,338 + MMP_RS00740 0.60 +0.6 135,421 - MMP_RS00740 0.75 -0.4 135,422 + MMP_RS00740 0.75 +0.2 135,565 - -0.2 135,600 + -0.8 135,606 + +0.2 135,613 - -2.6 135,613 - -1.1 135,619 + -0.3 135,675 - -1.9
Or see this region's nucleotide sequence