Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00510 and MMP_RS00515 are separated by 20 nucleotides MMP_RS00515 and MMP_RS00520 are separated by 100 nucleotides MMP_RS00520 and MMP_RS00525 are separated by 48 nucleotides MMP_RS00525 and MMP_RS00530 are separated by 85 nucleotides
MMP_RS00510: MMP_RS00510 - glycosyltransferase family 4 protein, at 100,750 to 101,817
_RS00510
MMP_RS00515: MMP_RS00515 - DUF655 domain-containing protein, at 101,838 to 102,422
_RS00515
MMP_RS00520: MMP_RS00520 - RNA polymerase Rpb4 family protein, at 102,523 to 102,846
_RS00520
MMP_RS00525: MMP_RS00525 - 50S ribosomal protein L21e, at 102,895 to 103,188
_RS00525
MMP_RS00530: MMP_RS00530 - tRNA pseudouridine(54/55) synthase Pus10, at 103,274 to 104,686
_RS00530
Position (kb)
101
102
103 Strain fitness (log2 ratio)
-2
-1
0
1 at 101.209 kb on + strand at 101.318 kb on - strand at 101.319 kb on + strand at 101.612 kb on - strand at 101.729 kb on + strand at 101.793 kb on + strand at 101.793 kb on + strand at 101.795 kb on + strand at 102.041 kb on - strand, within MMP_RS00515 at 102.335 kb on - strand, within MMP_RS00515 at 102.373 kb on - strand at 102.379 kb on - strand at 102.454 kb on + strand at 102.460 kb on + strand at 102.463 kb on - strand at 102.463 kb on - strand at 102.479 kb on - strand at 102.541 kb on - strand at 102.622 kb on - strand, within MMP_RS00520 at 102.706 kb on - strand, within MMP_RS00520 at 102.758 kb on + strand, within MMP_RS00520 at 103.258 kb on - strand at 103.296 kb on - strand at 103.393 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 101,209 + -0.3 101,318 - -0.3 101,319 + -0.1 101,612 - -1.6 101,729 + -1.2 101,793 + -1.4 101,793 + +0.4 101,795 + -1.7 102,041 - MMP_RS00515 0.35 +0.0 102,335 - MMP_RS00515 0.85 -1.8 102,373 - -0.6 102,379 - -2.3 102,454 + -1.5 102,460 + -0.4 102,463 - -0.7 102,463 - -2.4 102,479 - -2.2 102,541 - -0.5 102,622 - MMP_RS00520 0.31 -1.2 102,706 - MMP_RS00520 0.56 +1.4 102,758 + MMP_RS00520 0.73 +0.5 103,258 - -1.3 103,296 - +0.1 103,393 - +0.5
Or see this region's nucleotide sequence