Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_592 and Pf1N1B4_593 are separated by 222 nucleotides Pf1N1B4_593 and Pf1N1B4_594 are separated by 3 nucleotides Pf1N1B4_594 and Pf1N1B4_595 overlap by 8 nucleotides
Pf1N1B4_592 - Glucose-selective porin OprB, at 682,990 to 684,336
_592
Pf1N1B4_593 - Glucose ABC transporter, ATP-binding subunit (EC 3.6.3.-), at 684,559 to 685,719
_593
Pf1N1B4_594 - Glucose ABC transport system, inner membrane component 2, at 685,723 to 686,568
_594
Pf1N1B4_595 - Glucose ABC transport system, inner membrane component 1, at 686,561 to 687,505
_595
Position (kb) 684
685
686 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 683.614 kb on - strand, within Pf1N1B4_592 at 683.672 kb on + strand, within Pf1N1B4_592 at 683.672 kb on + strand, within Pf1N1B4_592 at 683.690 kb on + strand, within Pf1N1B4_592 at 683.691 kb on - strand, within Pf1N1B4_592 at 683.691 kb on - strand, within Pf1N1B4_592 at 683.711 kb on + strand, within Pf1N1B4_592 at 683.711 kb on + strand, within Pf1N1B4_592 at 683.712 kb on - strand, within Pf1N1B4_592 at 683.712 kb on - strand, within Pf1N1B4_592 at 683.712 kb on - strand, within Pf1N1B4_592 at 683.712 kb on - strand, within Pf1N1B4_592 at 683.712 kb on - strand, within Pf1N1B4_592 at 683.919 kb on + strand, within Pf1N1B4_592 at 683.933 kb on + strand, within Pf1N1B4_592 at 683.933 kb on + strand, within Pf1N1B4_592 at 683.934 kb on - strand, within Pf1N1B4_592 at 683.988 kb on + strand, within Pf1N1B4_592 at 683.988 kb on + strand, within Pf1N1B4_592 at 684.099 kb on - strand, within Pf1N1B4_592 at 684.123 kb on - strand, within Pf1N1B4_592 at 684.158 kb on + strand, within Pf1N1B4_592 at 684.178 kb on - strand, within Pf1N1B4_592 at 684.309 kb on + strand at 684.309 kb on + strand at 684.309 kb on + strand at 684.310 kb on - strand at 684.340 kb on + strand at 684.340 kb on + strand at 684.341 kb on - strand at 684.355 kb on - strand at 684.360 kb on + strand at 684.360 kb on + strand at 684.363 kb on + strand at 684.364 kb on - strand at 684.373 kb on + strand at 684.377 kb on + strand at 684.378 kb on - strand at 684.378 kb on - strand at 684.388 kb on - strand at 684.509 kb on - strand at 684.557 kb on - strand at 684.580 kb on + strand at 684.969 kb on + strand, within Pf1N1B4_593 at 685.049 kb on + strand, within Pf1N1B4_593 at 685.049 kb on + strand at 685.049 kb on + strand, within Pf1N1B4_593 at 685.050 kb on - strand, within Pf1N1B4_593 at 685.050 kb on - strand, within Pf1N1B4_593 at 685.050 kb on - strand, within Pf1N1B4_593 at 685.050 kb on - strand, within Pf1N1B4_593 at 685.050 kb on - strand, within Pf1N1B4_593 at 685.050 kb on - strand, within Pf1N1B4_593 at 685.050 kb on - strand, within Pf1N1B4_593 at 685.148 kb on + strand, within Pf1N1B4_593 at 685.148 kb on + strand, within Pf1N1B4_593 at 685.251 kb on - strand, within Pf1N1B4_593 at 685.251 kb on - strand, within Pf1N1B4_593 at 685.590 kb on + strand, within Pf1N1B4_593 at 685.591 kb on - strand, within Pf1N1B4_593 at 685.591 kb on - strand, within Pf1N1B4_593 at 685.682 kb on + strand at 685.682 kb on + strand at 685.682 kb on + strand at 685.683 kb on - strand at 685.702 kb on + strand at 685.703 kb on - strand at 685.703 kb on - strand at 685.763 kb on + strand at 685.764 kb on - strand at 685.777 kb on + strand at 685.778 kb on - strand at 685.778 kb on - strand at 685.781 kb on + strand at 685.781 kb on + strand at 685.781 kb on + strand at 685.781 kb on + strand at 685.782 kb on - strand at 685.835 kb on + strand, within Pf1N1B4_594 at 685.835 kb on + strand, within Pf1N1B4_594 at 685.835 kb on + strand, within Pf1N1B4_594 at 685.835 kb on + strand, within Pf1N1B4_594 at 685.836 kb on - strand, within Pf1N1B4_594 at 685.836 kb on - strand, within Pf1N1B4_594 at 686.075 kb on + strand, within Pf1N1B4_594 at 686.075 kb on + strand, within Pf1N1B4_594 at 686.075 kb on + strand, within Pf1N1B4_594 at 686.075 kb on + strand, within Pf1N1B4_594 at 686.076 kb on - strand, within Pf1N1B4_594 at 686.076 kb on - strand, within Pf1N1B4_594 at 686.076 kb on - strand, within Pf1N1B4_594 at 686.076 kb on - strand, within Pf1N1B4_594 at 686.078 kb on + strand, within Pf1N1B4_594 at 686.078 kb on + strand, within Pf1N1B4_594 at 686.078 kb on + strand, within Pf1N1B4_594 at 686.078 kb on + strand at 686.079 kb on - strand, within Pf1N1B4_594 at 686.079 kb on - strand, within Pf1N1B4_594 at 686.079 kb on - strand, within Pf1N1B4_594 at 686.279 kb on + strand, within Pf1N1B4_594 at 686.280 kb on - strand at 686.304 kb on + strand, within Pf1N1B4_594 at 686.304 kb on + strand, within Pf1N1B4_594 at 686.305 kb on - strand, within Pf1N1B4_594 at 686.305 kb on - strand, within Pf1N1B4_594 at 686.305 kb on - strand, within Pf1N1B4_594 at 686.486 kb on + strand at 686.486 kb on + strand at 686.486 kb on + strand at 686.487 kb on - strand at 686.487 kb on - strand at 686.487 kb on - strand at 686.487 kb on - strand at 686.516 kb on + strand at 686.516 kb on + strand at 686.516 kb on + strand at 686.516 kb on + strand at 686.516 kb on + strand at 686.532 kb on + strand at 686.563 kb on - strand at 686.688 kb on + strand, within Pf1N1B4_595 at 686.688 kb on + strand, within Pf1N1B4_595 at 686.688 kb on + strand, within Pf1N1B4_595 at 686.689 kb on - strand, within Pf1N1B4_595 at 686.718 kb on + strand, within Pf1N1B4_595 at 686.719 kb on - strand, within Pf1N1B4_595
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 683,614 - Pf1N1B4_592 0.46 -0.5 683,672 + Pf1N1B4_592 0.51 -0.6 683,672 + Pf1N1B4_592 0.51 +1.0 683,690 + Pf1N1B4_592 0.52 +1.3 683,691 - Pf1N1B4_592 0.52 -2.0 683,691 - Pf1N1B4_592 0.52 -0.8 683,711 + Pf1N1B4_592 0.54 -0.7 683,711 + Pf1N1B4_592 0.54 -1.1 683,712 - Pf1N1B4_592 0.54 -0.8 683,712 - Pf1N1B4_592 0.54 -1.5 683,712 - Pf1N1B4_592 0.54 +0.6 683,712 - Pf1N1B4_592 0.54 +0.6 683,712 - Pf1N1B4_592 0.54 +0.5 683,919 + Pf1N1B4_592 0.69 -0.4 683,933 + Pf1N1B4_592 0.70 -3.0 683,933 + Pf1N1B4_592 0.70 -2.2 683,934 - Pf1N1B4_592 0.70 -0.4 683,988 + Pf1N1B4_592 0.74 +0.4 683,988 + Pf1N1B4_592 0.74 -0.0 684,099 - Pf1N1B4_592 0.82 +0.7 684,123 - Pf1N1B4_592 0.84 -1.7 684,158 + Pf1N1B4_592 0.87 -0.5 684,178 - Pf1N1B4_592 0.88 -0.2 684,309 + -1.4 684,309 + +0.1 684,309 + -1.2 684,310 - +0.4 684,340 + -1.3 684,340 + +0.9 684,341 - +0.3 684,355 - +0.8 684,360 + -1.3 684,360 + -1.5 684,363 + +0.0 684,364 - +2.2 684,373 + +0.3 684,377 + -0.4 684,378 - -0.5 684,378 - +1.0 684,388 - +1.6 684,509 - -0.4 684,557 - +1.4 684,580 + -1.7 684,969 + Pf1N1B4_593 0.35 -0.6 685,049 + Pf1N1B4_593 0.42 -2.8 685,049 + -1.4 685,049 + Pf1N1B4_593 0.42 -2.4 685,050 - Pf1N1B4_593 0.42 -1.7 685,050 - Pf1N1B4_593 0.42 -1.9 685,050 - Pf1N1B4_593 0.42 -1.7 685,050 - Pf1N1B4_593 0.42 -3.9 685,050 - Pf1N1B4_593 0.42 -1.8 685,050 - Pf1N1B4_593 0.42 -0.9 685,050 - Pf1N1B4_593 0.42 -1.0 685,148 + Pf1N1B4_593 0.51 -0.9 685,148 + Pf1N1B4_593 0.51 -1.4 685,251 - Pf1N1B4_593 0.60 -1.4 685,251 - Pf1N1B4_593 0.60 -2.3 685,590 + Pf1N1B4_593 0.89 -1.4 685,591 - Pf1N1B4_593 0.89 -2.4 685,591 - Pf1N1B4_593 0.89 -0.7 685,682 + -2.7 685,682 + -1.7 685,682 + -1.6 685,683 - +1.3 685,702 + -2.5 685,703 - +0.5 685,703 - -1.4 685,763 + -1.9 685,764 - -2.6 685,777 + -1.4 685,778 - -1.9 685,778 - -1.1 685,781 + -3.3 685,781 + -2.1 685,781 + -1.9 685,781 + -0.0 685,782 - +0.7 685,835 + Pf1N1B4_594 0.13 -2.8 685,835 + Pf1N1B4_594 0.13 -2.8 685,835 + Pf1N1B4_594 0.13 -0.7 685,835 + Pf1N1B4_594 0.13 -2.7 685,836 - Pf1N1B4_594 0.13 -1.8 685,836 - Pf1N1B4_594 0.13 -2.2 686,075 + Pf1N1B4_594 0.42 -1.3 686,075 + Pf1N1B4_594 0.42 -1.8 686,075 + Pf1N1B4_594 0.42 -3.0 686,075 + Pf1N1B4_594 0.42 -1.7 686,076 - Pf1N1B4_594 0.42 -1.6 686,076 - Pf1N1B4_594 0.42 -2.8 686,076 - Pf1N1B4_594 0.42 -1.9 686,076 - Pf1N1B4_594 0.42 -2.1 686,078 + Pf1N1B4_594 0.42 -2.3 686,078 + Pf1N1B4_594 0.42 -2.7 686,078 + Pf1N1B4_594 0.42 -1.7 686,078 + -0.5 686,079 - Pf1N1B4_594 0.42 -0.8 686,079 - Pf1N1B4_594 0.42 -1.8 686,079 - Pf1N1B4_594 0.42 -1.3 686,279 + Pf1N1B4_594 0.66 -2.5 686,280 - -0.7 686,304 + Pf1N1B4_594 0.69 -2.0 686,304 + Pf1N1B4_594 0.69 +0.3 686,305 - Pf1N1B4_594 0.69 -1.6 686,305 - Pf1N1B4_594 0.69 -1.3 686,305 - Pf1N1B4_594 0.69 +0.3 686,486 + -1.6 686,486 + -1.1 686,486 + -1.7 686,487 - -2.1 686,487 - -1.9 686,487 - -2.5 686,487 - -2.9 686,516 + -2.3 686,516 + -2.5 686,516 + -2.5 686,516 + -1.9 686,516 + -3.8 686,532 + -2.9 686,563 - -1.3 686,688 + Pf1N1B4_595 0.13 -2.6 686,688 + Pf1N1B4_595 0.13 -1.3 686,688 + Pf1N1B4_595 0.13 -2.2 686,689 - Pf1N1B4_595 0.14 -1.5 686,718 + Pf1N1B4_595 0.17 -3.0 686,719 - Pf1N1B4_595 0.17 -2.8
Or see this region's nucleotide sequence