Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5905 and Pf1N1B4_5906 are separated by 162 nucleotides Pf1N1B4_5906 and Pf1N1B4_5907 are separated by 63 nucleotides
Pf1N1B4_5905 - Transcriptional regulator, LysR family, at 347,761 to 348,666
_5905
Pf1N1B4_5906 - Esterase/lipase, at 348,829 to 349,845
_5906
Pf1N1B4_5907 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 349,909 to 351,336
_5907
Position (kb) 348
349
350 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 347.957 kb on + strand, within Pf1N1B4_5905 at 348.115 kb on + strand, within Pf1N1B4_5905 at 348.116 kb on - strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.251 kb on + strand, within Pf1N1B4_5905 at 348.473 kb on + strand, within Pf1N1B4_5905 at 348.473 kb on + strand at 348.474 kb on - strand, within Pf1N1B4_5905 at 348.589 kb on + strand at 348.589 kb on + strand at 348.590 kb on - strand at 348.642 kb on + strand at 348.643 kb on - strand at 348.643 kb on - strand at 348.643 kb on - strand at 348.648 kb on + strand at 348.812 kb on - strand at 348.813 kb on + strand at 348.814 kb on - strand at 348.814 kb on - strand at 348.814 kb on - strand at 349.234 kb on + strand, within Pf1N1B4_5906 at 349.235 kb on - strand, within Pf1N1B4_5906 at 349.292 kb on - strand, within Pf1N1B4_5906 at 349.553 kb on - strand, within Pf1N1B4_5906 at 349.688 kb on - strand, within Pf1N1B4_5906 at 349.736 kb on - strand, within Pf1N1B4_5906 at 349.805 kb on + strand at 349.844 kb on - strand at 349.844 kb on - strand at 349.844 kb on - strand at 350.138 kb on + strand, within Pf1N1B4_5907 at 350.138 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.281 kb on + strand, within Pf1N1B4_5907 at 350.361 kb on + strand, within Pf1N1B4_5907 at 350.361 kb on + strand, within Pf1N1B4_5907 at 350.728 kb on + strand, within Pf1N1B4_5907
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 347,957 + Pf1N1B4_5905 0.22 +0.0 348,115 + Pf1N1B4_5905 0.39 +0.2 348,116 - Pf1N1B4_5905 0.39 +0.3 348,155 + Pf1N1B4_5905 0.43 +0.1 348,155 + Pf1N1B4_5905 0.43 -2.3 348,155 + Pf1N1B4_5905 0.43 +0.3 348,251 + Pf1N1B4_5905 0.54 -1.7 348,473 + Pf1N1B4_5905 0.79 -2.3 348,473 + +1.3 348,474 - Pf1N1B4_5905 0.79 +0.6 348,589 + +0.9 348,589 + -1.3 348,590 - +1.3 348,642 + -1.4 348,643 - +0.3 348,643 - +0.4 348,643 - -1.1 348,648 + +0.7 348,812 - -2.8 348,813 + -1.3 348,814 - +0.3 348,814 - -0.3 348,814 - +0.5 349,234 + Pf1N1B4_5906 0.40 -0.4 349,235 - Pf1N1B4_5906 0.40 +1.3 349,292 - Pf1N1B4_5906 0.46 +0.6 349,553 - Pf1N1B4_5906 0.71 -0.7 349,688 - Pf1N1B4_5906 0.84 -0.9 349,736 - Pf1N1B4_5906 0.89 -0.9 349,805 + -1.4 349,844 - +0.4 349,844 - +0.7 349,844 - -2.2 350,138 + Pf1N1B4_5907 0.16 +1.0 350,138 + Pf1N1B4_5907 0.16 +0.0 350,204 + Pf1N1B4_5907 0.21 +2.1 350,204 + Pf1N1B4_5907 0.21 +1.0 350,204 + Pf1N1B4_5907 0.21 +1.8 350,205 - Pf1N1B4_5907 0.21 +1.1 350,205 - Pf1N1B4_5907 0.21 +0.0 350,205 - Pf1N1B4_5907 0.21 -1.7 350,281 + Pf1N1B4_5907 0.26 +0.7 350,361 + Pf1N1B4_5907 0.32 -2.6 350,361 + Pf1N1B4_5907 0.32 +1.7 350,728 + Pf1N1B4_5907 0.57 +1.2
Or see this region's nucleotide sequence