Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5822 and Pf1N1B4_5823 overlap by 1 nucleotides Pf1N1B4_5823 and Pf1N1B4_5824 are separated by 5 nucleotides Pf1N1B4_5824 and Pf1N1B4_5825 overlap by 4 nucleotides Pf1N1B4_5825 and Pf1N1B4_5826 overlap by 4 nucleotides Pf1N1B4_5826 and Pf1N1B4_5827 are separated by 8 nucleotides
Pf1N1B4_5822 - Na(+) H(+) antiporter subunit A / Na(+) H(+) antiporter subunit B, at 256,081 to 258,957
_5822
Pf1N1B4_5823 - Na(+) H(+) antiporter subunit C, at 258,957 to 259,301
_5823
Pf1N1B4_5824 - Na(+) H(+) antiporter subunit D, at 259,307 to 260,983
_5824
Pf1N1B4_5825 - Na(+) H(+) antiporter subunit E, at 260,980 to 261,468
_5825
Pf1N1B4_5826 - Na(+) H(+) antiporter subunit F, at 261,465 to 261,743
_5826
Pf1N1B4_5827 - Na(+) H(+) antiporter subunit G, at 261,752 to 262,102
_5827
Position (kb) 259
260
261 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.620 kb on - strand, within Pf1N1B4_5822 at 258.829 kb on + strand at 258.898 kb on - strand at 259.065 kb on + strand, within Pf1N1B4_5823 at 259.292 kb on + strand at 259.496 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.800 kb on - strand, within Pf1N1B4_5824 at 259.856 kb on + strand, within Pf1N1B4_5824 at 260.078 kb on + strand, within Pf1N1B4_5824 at 260.315 kb on + strand, within Pf1N1B4_5824 at 260.316 kb on - strand, within Pf1N1B4_5824 at 260.906 kb on + strand at 260.906 kb on + strand at 260.906 kb on + strand at 260.906 kb on + strand at 260.907 kb on - strand at 260.907 kb on - strand at 260.907 kb on - strand at 261.068 kb on + strand, within Pf1N1B4_5825 at 261.595 kb on - strand, within Pf1N1B4_5826 at 261.595 kb on - strand, within Pf1N1B4_5826 at 261.595 kb on - strand, within Pf1N1B4_5826 at 261.808 kb on + strand, within Pf1N1B4_5827 at 261.923 kb on + strand, within Pf1N1B4_5827
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 258,619 + Pf1N1B4_5822 0.88 -2.4 258,619 + Pf1N1B4_5822 0.88 -1.8 258,619 + Pf1N1B4_5822 0.88 -1.1 258,620 - Pf1N1B4_5822 0.88 -0.9 258,829 + -2.3 258,898 - +1.1 259,065 + Pf1N1B4_5823 0.31 -1.1 259,292 + -0.8 259,496 + Pf1N1B4_5824 0.11 -1.6 259,799 + Pf1N1B4_5824 0.29 -2.8 259,799 + Pf1N1B4_5824 0.29 -4.2 259,799 + Pf1N1B4_5824 0.29 -1.7 259,799 + -0.9 259,799 + Pf1N1B4_5824 0.29 -0.9 259,799 + Pf1N1B4_5824 0.29 -2.9 259,799 + Pf1N1B4_5824 0.29 -1.8 259,800 - Pf1N1B4_5824 0.29 -1.6 259,856 + Pf1N1B4_5824 0.33 -1.2 260,078 + Pf1N1B4_5824 0.46 -0.7 260,315 + Pf1N1B4_5824 0.60 -2.6 260,316 - Pf1N1B4_5824 0.60 -2.9 260,906 + -1.8 260,906 + -3.3 260,906 + -2.2 260,906 + +0.6 260,907 - -0.9 260,907 - -2.6 260,907 - -1.2 261,068 + Pf1N1B4_5825 0.18 -2.2 261,595 - Pf1N1B4_5826 0.47 -2.4 261,595 - Pf1N1B4_5826 0.47 -0.5 261,595 - Pf1N1B4_5826 0.47 -1.8 261,808 + Pf1N1B4_5827 0.16 -1.8 261,923 + Pf1N1B4_5827 0.49 -3.4
Or see this region's nucleotide sequence