Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5375 and Pf1N1B4_5376 are separated by 164 nucleotides Pf1N1B4_5376 and Pf1N1B4_5377 are separated by 206 nucleotides
Pf1N1B4_5375 - putative DNA methyltransferase, at 107,874 to 109,142
_5375
Pf1N1B4_5376 - hypothetical protein, at 109,307 to 109,810
_5376
Pf1N1B4_5377 - Uncharacterized domain COG3236 / GTP cyclohydrolase II (EC 3.5.4.25), at 110,017 to 110,511
_5377
Position (kb)
109
110 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 108.487 kb on + strand, within Pf1N1B4_5375 at 108.487 kb on + strand, within Pf1N1B4_5375 at 108.487 kb on + strand, within Pf1N1B4_5375 at 108.487 kb on + strand, within Pf1N1B4_5375 at 108.487 kb on + strand, within Pf1N1B4_5375 at 108.487 kb on + strand, within Pf1N1B4_5375 at 108.488 kb on - strand, within Pf1N1B4_5375 at 108.488 kb on - strand, within Pf1N1B4_5375 at 108.488 kb on - strand, within Pf1N1B4_5375 at 108.535 kb on + strand, within Pf1N1B4_5375 at 108.535 kb on + strand, within Pf1N1B4_5375 at 108.535 kb on + strand, within Pf1N1B4_5375 at 108.535 kb on + strand, within Pf1N1B4_5375 at 108.535 kb on + strand, within Pf1N1B4_5375 at 108.535 kb on + strand, within Pf1N1B4_5375 at 108.536 kb on - strand, within Pf1N1B4_5375 at 108.539 kb on + strand, within Pf1N1B4_5375 at 108.539 kb on + strand, within Pf1N1B4_5375 at 108.540 kb on - strand, within Pf1N1B4_5375 at 108.540 kb on - strand, within Pf1N1B4_5375 at 108.540 kb on - strand at 108.569 kb on + strand, within Pf1N1B4_5375 at 108.591 kb on + strand, within Pf1N1B4_5375 at 108.591 kb on + strand, within Pf1N1B4_5375 at 108.591 kb on + strand, within Pf1N1B4_5375 at 108.609 kb on + strand, within Pf1N1B4_5375 at 108.679 kb on + strand, within Pf1N1B4_5375 at 108.794 kb on + strand, within Pf1N1B4_5375 at 108.814 kb on - strand, within Pf1N1B4_5375 at 108.989 kb on - strand, within Pf1N1B4_5375 at 109.078 kb on + strand at 109.078 kb on + strand at 109.078 kb on + strand at 109.079 kb on - strand at 109.213 kb on + strand at 109.213 kb on + strand at 109.214 kb on - strand at 109.300 kb on - strand at 109.300 kb on - strand at 109.357 kb on + strand at 109.358 kb on - strand, within Pf1N1B4_5376 at 109.361 kb on - strand, within Pf1N1B4_5376 at 109.482 kb on + strand, within Pf1N1B4_5376 at 109.483 kb on - strand, within Pf1N1B4_5376 at 109.483 kb on - strand, within Pf1N1B4_5376 at 109.612 kb on + strand, within Pf1N1B4_5376 at 109.777 kb on + strand at 109.791 kb on + strand at 109.791 kb on + strand at 109.791 kb on + strand at 109.791 kb on + strand at 109.792 kb on - strand at 109.792 kb on - strand at 109.792 kb on - strand at 109.792 kb on - strand at 109.792 kb on - strand at 109.792 kb on - strand at 109.966 kb on - strand at 109.966 kb on - strand at 109.966 kb on - strand at 109.966 kb on - strand at 110.005 kb on + strand at 110.005 kb on + strand at 110.005 kb on + strand at 110.019 kb on - strand at 110.154 kb on - strand, within Pf1N1B4_5377 at 110.170 kb on + strand, within Pf1N1B4_5377 at 110.263 kb on - strand, within Pf1N1B4_5377 at 110.289 kb on - strand, within Pf1N1B4_5377 at 110.390 kb on + strand, within Pf1N1B4_5377 at 110.391 kb on - strand, within Pf1N1B4_5377 at 110.494 kb on - strand at 110.494 kb on - strand at 110.494 kb on - strand at 110.580 kb on + strand at 110.581 kb on - strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.609 kb on + strand at 110.609 kb on + strand at 110.609 kb on + strand at 110.609 kb on + strand at 110.610 kb on - strand at 110.610 kb on - strand at 110.653 kb on + strand at 110.653 kb on + strand at 110.653 kb on + strand at 110.654 kb on - strand at 110.654 kb on - strand at 110.689 kb on + strand at 110.690 kb on - strand at 110.697 kb on + strand at 110.697 kb on + strand at 110.697 kb on + strand at 110.698 kb on - strand at 110.698 kb on - strand at 110.698 kb on - strand at 110.698 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 108,487 + Pf1N1B4_5375 0.48 -0.6 108,487 + Pf1N1B4_5375 0.48 +1.4 108,487 + Pf1N1B4_5375 0.48 +2.6 108,487 + Pf1N1B4_5375 0.48 -2.6 108,487 + Pf1N1B4_5375 0.48 -0.5 108,487 + Pf1N1B4_5375 0.48 -1.0 108,488 - Pf1N1B4_5375 0.48 +0.7 108,488 - Pf1N1B4_5375 0.48 -1.4 108,488 - Pf1N1B4_5375 0.48 +1.7 108,535 + Pf1N1B4_5375 0.52 -1.0 108,535 + Pf1N1B4_5375 0.52 +0.9 108,535 + Pf1N1B4_5375 0.52 -0.2 108,535 + Pf1N1B4_5375 0.52 +1.8 108,535 + Pf1N1B4_5375 0.52 +0.4 108,535 + Pf1N1B4_5375 0.52 -1.0 108,536 - Pf1N1B4_5375 0.52 +2.0 108,539 + Pf1N1B4_5375 0.52 +1.6 108,539 + Pf1N1B4_5375 0.52 +1.5 108,540 - Pf1N1B4_5375 0.52 +0.7 108,540 - Pf1N1B4_5375 0.52 -0.8 108,540 - +1.0 108,569 + Pf1N1B4_5375 0.55 +2.2 108,591 + Pf1N1B4_5375 0.57 +1.5 108,591 + Pf1N1B4_5375 0.57 -2.2 108,591 + Pf1N1B4_5375 0.57 +0.2 108,609 + Pf1N1B4_5375 0.58 +1.0 108,679 + Pf1N1B4_5375 0.63 +1.5 108,794 + Pf1N1B4_5375 0.72 -1.8 108,814 - Pf1N1B4_5375 0.74 -0.9 108,989 - Pf1N1B4_5375 0.88 -0.1 109,078 + -1.8 109,078 + +0.7 109,078 + +1.3 109,079 - -1.4 109,213 + -1.8 109,213 + -0.4 109,214 - -2.1 109,300 - -1.9 109,300 - -0.4 109,357 + -1.5 109,358 - Pf1N1B4_5376 0.10 -1.4 109,361 - Pf1N1B4_5376 0.11 +1.8 109,482 + Pf1N1B4_5376 0.35 -1.4 109,483 - Pf1N1B4_5376 0.35 -2.4 109,483 - Pf1N1B4_5376 0.35 -2.8 109,612 + Pf1N1B4_5376 0.61 -2.4 109,777 + +1.0 109,791 + -1.8 109,791 + -1.7 109,791 + -2.5 109,791 + -0.8 109,792 - +1.6 109,792 - +1.2 109,792 - +2.0 109,792 - +1.1 109,792 - +0.5 109,792 - -0.3 109,966 - -0.1 109,966 - +1.0 109,966 - +0.8 109,966 - -0.4 110,005 + +0.6 110,005 + -1.8 110,005 + +0.2 110,019 - +1.5 110,154 - Pf1N1B4_5377 0.28 -1.1 110,170 + Pf1N1B4_5377 0.31 -0.2 110,263 - Pf1N1B4_5377 0.50 +0.2 110,289 - Pf1N1B4_5377 0.55 -0.2 110,390 + Pf1N1B4_5377 0.75 -2.0 110,391 - Pf1N1B4_5377 0.76 +0.6 110,494 - +0.8 110,494 - -0.5 110,494 - -0.6 110,580 + -0.4 110,581 - -1.0 110,605 + -2.9 110,605 + +0.2 110,605 + -0.8 110,605 + +1.0 110,605 + +0.4 110,605 + -0.3 110,606 - +0.4 110,606 - -1.2 110,606 - -0.3 110,606 - -0.4 110,606 - -0.0 110,606 - -0.0 110,606 - -0.8 110,606 - +0.6 110,606 - +0.1 110,609 + -0.6 110,609 + -0.6 110,609 + +1.2 110,609 + -0.2 110,610 - -2.0 110,610 - -1.2 110,653 + -1.0 110,653 + -0.4 110,653 + -2.2 110,654 - -1.5 110,654 - -2.0 110,689 + -0.7 110,690 - +1.1 110,697 + -2.2 110,697 + +0.7 110,697 + -1.0 110,698 - -1.0 110,698 - -0.0 110,698 - +0.1 110,698 - -1.4
Or see this region's nucleotide sequence