Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_47 and Pf1N1B4_48 are separated by 145 nucleotides Pf1N1B4_48 and Pf1N1B4_49 are separated by 30 nucleotides Pf1N1B4_49 and Pf1N1B4_50 are separated by 163 nucleotides Pf1N1B4_50 and Pf1N1B4_51 are separated by 89 nucleotides
Pf1N1B4_47 - Copper resistance protein D, at 54,921 to 55,850
_47
Pf1N1B4_48 - hypothetical protein, at 55,996 to 56,406
_48
Pf1N1B4_49 - hypothetical protein, at 56,437 to 56,643
_49
Pf1N1B4_50 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 56,807 to 56,989
_50
Pf1N1B4_51 - hypothetical protein, at 57,079 to 57,348
_51
Position (kb) 56
57 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 55.499 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.612 kb on - strand, within Pf1N1B4_47 at 55.848 kb on + strand at 55.848 kb on + strand at 55.863 kb on + strand at 55.863 kb on + strand at 55.864 kb on - strand at 55.870 kb on + strand at 55.980 kb on + strand at 55.980 kb on + strand at 55.981 kb on - strand at 56.005 kb on + strand at 56.006 kb on - strand at 56.516 kb on + strand, within Pf1N1B4_49 at 56.517 kb on - strand, within Pf1N1B4_49 at 56.520 kb on - strand, within Pf1N1B4_49 at 56.559 kb on + strand, within Pf1N1B4_49 at 56.559 kb on + strand, within Pf1N1B4_49 at 56.560 kb on - strand, within Pf1N1B4_49 at 56.560 kb on - strand at 56.560 kb on - strand, within Pf1N1B4_49 at 56.588 kb on + strand, within Pf1N1B4_49 at 56.588 kb on + strand, within Pf1N1B4_49 at 56.767 kb on + strand at 56.784 kb on + strand at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.896 kb on - strand at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 57.074 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51 at 57.409 kb on + strand at 57.432 kb on - strand at 57.504 kb on - strand at 57.512 kb on + strand at 57.512 kb on + strand at 57.513 kb on - strand at 57.603 kb on - strand at 57.603 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 55,499 + Pf1N1B4_47 0.62 -2.4 55,611 + Pf1N1B4_47 0.74 +0.3 55,611 + Pf1N1B4_47 0.74 +1.1 55,611 + Pf1N1B4_47 0.74 +1.1 55,611 + Pf1N1B4_47 0.74 +0.2 55,612 - Pf1N1B4_47 0.74 -1.2 55,848 + -2.9 55,848 + -2.5 55,863 + -0.5 55,863 + +0.0 55,864 - -0.1 55,870 + +0.0 55,980 + -0.3 55,980 + +0.2 55,981 - +1.2 56,005 + -2.4 56,006 - +1.2 56,516 + Pf1N1B4_49 0.38 -1.5 56,517 - Pf1N1B4_49 0.39 -1.0 56,520 - Pf1N1B4_49 0.40 -1.2 56,559 + Pf1N1B4_49 0.59 -2.1 56,559 + Pf1N1B4_49 0.59 -1.0 56,560 - Pf1N1B4_49 0.59 +1.2 56,560 - +1.2 56,560 - Pf1N1B4_49 0.59 -0.4 56,588 + Pf1N1B4_49 0.73 -2.7 56,588 + Pf1N1B4_49 0.73 +0.4 56,767 + -0.5 56,784 + +1.8 56,837 + Pf1N1B4_50 0.16 -1.0 56,837 + Pf1N1B4_50 0.16 +1.3 56,837 + Pf1N1B4_50 0.16 -0.7 56,837 + Pf1N1B4_50 0.16 +0.1 56,838 - Pf1N1B4_50 0.17 +0.5 56,838 - Pf1N1B4_50 0.17 +0.1 56,838 - Pf1N1B4_50 0.17 -1.4 56,838 - +0.9 56,895 + Pf1N1B4_50 0.48 +1.1 56,895 + -0.9 56,895 + Pf1N1B4_50 0.48 -1.5 56,895 + Pf1N1B4_50 0.48 +0.3 56,895 + Pf1N1B4_50 0.48 -0.3 56,895 + Pf1N1B4_50 0.48 +1.9 56,896 - -0.7 56,896 - Pf1N1B4_50 0.49 +1.6 56,896 - Pf1N1B4_50 0.49 -0.1 56,896 - Pf1N1B4_50 0.49 -0.9 56,896 - Pf1N1B4_50 0.49 -0.4 57,074 + +0.7 57,087 + +0.0 57,087 + -1.7 57,087 + +0.1 57,087 + +1.6 57,221 + Pf1N1B4_51 0.53 +0.9 57,221 + Pf1N1B4_51 0.53 -1.6 57,221 + +1.5 57,221 + Pf1N1B4_51 0.53 +0.1 57,221 + Pf1N1B4_51 0.53 -1.2 57,221 + Pf1N1B4_51 0.53 +0.7 57,221 + Pf1N1B4_51 0.53 -1.8 57,222 - Pf1N1B4_51 0.53 +1.5 57,222 - Pf1N1B4_51 0.53 -2.5 57,409 + -1.2 57,432 - +0.6 57,504 - +0.3 57,512 + -0.7 57,512 + +0.9 57,513 - -1.1 57,603 - -0.1 57,603 - -1.1
Or see this region's nucleotide sequence