Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4747 and Pf1N1B4_4748 are separated by 121 nucleotides Pf1N1B4_4748 and Pf1N1B4_4749 are separated by 246 nucleotides
Pf1N1B4_4747 - Probable lipoprotein signal peptide, at 5,242,185 to 5,243,222
_4747
Pf1N1B4_4748 - transporter, putative, at 5,243,344 to 5,244,531
_4748
Pf1N1B4_4749 - ABC transporter ATP-binding protein uup, at 5,244,778 to 5,246,343
_4749
Position (kb)
5243
5244
5245 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5242.901 kb on + strand, within Pf1N1B4_4747 at 5242.902 kb on - strand at 5243.047 kb on + strand, within Pf1N1B4_4747 at 5243.048 kb on - strand at 5243.220 kb on + strand at 5243.281 kb on + strand at 5243.282 kb on - strand at 5243.282 kb on - strand at 5243.288 kb on - strand at 5243.288 kb on - strand at 5243.288 kb on - strand at 5243.288 kb on - strand at 5243.346 kb on - strand at 5243.346 kb on - strand at 5243.502 kb on - strand, within Pf1N1B4_4748 at 5243.772 kb on - strand, within Pf1N1B4_4748 at 5243.801 kb on + strand, within Pf1N1B4_4748 at 5243.801 kb on + strand, within Pf1N1B4_4748 at 5243.801 kb on + strand, within Pf1N1B4_4748 at 5243.802 kb on - strand, within Pf1N1B4_4748 at 5243.802 kb on - strand, within Pf1N1B4_4748 at 5243.802 kb on - strand, within Pf1N1B4_4748 at 5243.805 kb on - strand, within Pf1N1B4_4748 at 5243.814 kb on + strand, within Pf1N1B4_4748 at 5243.815 kb on - strand, within Pf1N1B4_4748 at 5243.815 kb on - strand, within Pf1N1B4_4748 at 5243.820 kb on + strand at 5243.821 kb on - strand, within Pf1N1B4_4748 at 5244.461 kb on + strand at 5244.461 kb on + strand at 5244.462 kb on - strand at 5244.462 kb on - strand at 5244.462 kb on - strand at 5244.462 kb on - strand at 5244.587 kb on + strand at 5244.780 kb on - strand at 5244.780 kb on - strand at 5244.780 kb on - strand at 5244.780 kb on - strand at 5244.807 kb on - strand at 5244.815 kb on + strand at 5244.815 kb on + strand at 5244.816 kb on - strand at 5245.209 kb on - strand, within Pf1N1B4_4749 at 5245.212 kb on - strand, within Pf1N1B4_4749
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 5,242,901 + Pf1N1B4_4747 0.69 +2.4 5,242,902 - +1.0 5,243,047 + Pf1N1B4_4747 0.83 +0.8 5,243,048 - +0.8 5,243,220 + -0.7 5,243,281 + -1.9 5,243,282 - +0.6 5,243,282 - -1.4 5,243,288 - -1.0 5,243,288 - +1.0 5,243,288 - +1.0 5,243,288 - -0.1 5,243,346 - -1.0 5,243,346 - +1.8 5,243,502 - Pf1N1B4_4748 0.13 +2.0 5,243,772 - Pf1N1B4_4748 0.36 +1.2 5,243,801 + Pf1N1B4_4748 0.38 -0.6 5,243,801 + Pf1N1B4_4748 0.38 -2.1 5,243,801 + Pf1N1B4_4748 0.38 -3.4 5,243,802 - Pf1N1B4_4748 0.39 +1.0 5,243,802 - Pf1N1B4_4748 0.39 +1.3 5,243,802 - Pf1N1B4_4748 0.39 -2.0 5,243,805 - Pf1N1B4_4748 0.39 +2.7 5,243,814 + Pf1N1B4_4748 0.40 +0.4 5,243,815 - Pf1N1B4_4748 0.40 -2.2 5,243,815 - Pf1N1B4_4748 0.40 +1.4 5,243,820 + -1.3 5,243,821 - Pf1N1B4_4748 0.40 -0.6 5,244,461 + +1.3 5,244,461 + -1.2 5,244,462 - +0.5 5,244,462 - -0.4 5,244,462 - +0.3 5,244,462 - -2.7 5,244,587 + -0.5 5,244,780 - +0.3 5,244,780 - +1.3 5,244,780 - +1.1 5,244,780 - -0.6 5,244,807 - +0.8 5,244,815 + -0.2 5,244,815 + -1.0 5,244,816 - +1.8 5,245,209 - Pf1N1B4_4749 0.28 -1.2 5,245,212 - Pf1N1B4_4749 0.28 +0.3
Or see this region's nucleotide sequence