Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4595 and Pf1N1B4_4596 are separated by 99 nucleotides Pf1N1B4_4596 and Pf1N1B4_4597 are separated by 65 nucleotides Pf1N1B4_4597 and Pf1N1B4_4598 are separated by 92 nucleotides
Pf1N1B4_4595 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 5,067,954 to 5,069,408
_4595
Pf1N1B4_4596 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 5,069,508 to 5,070,275
_4596
Pf1N1B4_4597 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 5,070,341 to 5,071,099
_4597
Pf1N1B4_4598 - Ribonuclease HI (EC 3.1.26.4), at 5,071,192 to 5,071,644
_4598
Position (kb)
5069
5070
5071 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5068.682 kb on - strand, within Pf1N1B4_4595 at 5068.891 kb on + strand, within Pf1N1B4_4595 at 5069.516 kb on + strand at 5069.785 kb on + strand, within Pf1N1B4_4596 at 5069.785 kb on + strand at 5069.917 kb on + strand, within Pf1N1B4_4596 at 5069.917 kb on + strand at 5069.917 kb on + strand, within Pf1N1B4_4596 at 5070.046 kb on + strand, within Pf1N1B4_4596 at 5070.046 kb on + strand, within Pf1N1B4_4596 at 5070.047 kb on - strand at 5070.047 kb on - strand at 5070.222 kb on + strand at 5070.222 kb on + strand at 5070.223 kb on - strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.235 kb on + strand at 5070.236 kb on - strand at 5070.270 kb on + strand at 5070.974 kb on + strand, within Pf1N1B4_4597 at 5070.978 kb on + strand at 5071.003 kb on - strand at 5071.097 kb on + strand at 5071.100 kb on + strand at 5071.101 kb on - strand at 5071.109 kb on + strand at 5071.205 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 5,068,682 - Pf1N1B4_4595 0.50 -1.6 5,068,891 + Pf1N1B4_4595 0.64 +1.2 5,069,516 + -0.1 5,069,785 + Pf1N1B4_4596 0.36 -0.8 5,069,785 + +0.2 5,069,917 + Pf1N1B4_4596 0.53 -0.7 5,069,917 + +0.3 5,069,917 + Pf1N1B4_4596 0.53 +1.1 5,070,046 + Pf1N1B4_4596 0.70 -2.1 5,070,046 + Pf1N1B4_4596 0.70 -2.6 5,070,047 - +0.7 5,070,047 - +0.3 5,070,222 + -0.6 5,070,222 + -1.4 5,070,223 - -0.2 5,070,235 + -1.7 5,070,235 + -1.0 5,070,235 + -0.1 5,070,235 + -0.1 5,070,235 + -2.6 5,070,235 + +0.4 5,070,235 + -1.7 5,070,236 - +1.2 5,070,270 + +1.1 5,070,974 + Pf1N1B4_4597 0.83 -1.7 5,070,978 + +0.3 5,071,003 - -0.4 5,071,097 + +0.1 5,071,100 + +2.3 5,071,101 - +2.6 5,071,109 + -0.4 5,071,205 + +0.9
Or see this region's nucleotide sequence