Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4596

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4595 and Pf1N1B4_4596 are separated by 99 nucleotidesPf1N1B4_4596 and Pf1N1B4_4597 are separated by 65 nucleotidesPf1N1B4_4597 and Pf1N1B4_4598 are separated by 92 nucleotides Pf1N1B4_4595 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 5,067,954 to 5,069,408 _4595 Pf1N1B4_4596 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 5,069,508 to 5,070,275 _4596 Pf1N1B4_4597 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 5,070,341 to 5,071,099 _4597 Pf1N1B4_4598 - Ribonuclease HI (EC 3.1.26.4), at 5,071,192 to 5,071,644 _4598 Position (kb) 5069 5070 5071Strain fitness (log2 ratio) -2 -1 0 1 2at 5068.682 kb on - strand, within Pf1N1B4_4595at 5068.891 kb on + strand, within Pf1N1B4_4595at 5069.516 kb on + strandat 5069.785 kb on + strand, within Pf1N1B4_4596at 5069.785 kb on + strandat 5069.917 kb on + strand, within Pf1N1B4_4596at 5069.917 kb on + strandat 5069.917 kb on + strand, within Pf1N1B4_4596at 5070.046 kb on + strand, within Pf1N1B4_4596at 5070.046 kb on + strand, within Pf1N1B4_4596at 5070.047 kb on - strandat 5070.047 kb on - strandat 5070.222 kb on + strandat 5070.222 kb on + strandat 5070.223 kb on - strandat 5070.235 kb on + strandat 5070.235 kb on + strandat 5070.235 kb on + strandat 5070.235 kb on + strandat 5070.235 kb on + strandat 5070.235 kb on + strandat 5070.235 kb on + strandat 5070.236 kb on - strandat 5070.270 kb on + strandat 5070.974 kb on + strand, within Pf1N1B4_4597at 5070.978 kb on + strandat 5071.003 kb on - strandat 5071.097 kb on + strandat 5071.100 kb on + strandat 5071.101 kb on - strandat 5071.109 kb on + strandat 5071.205 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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5,068,682 - Pf1N1B4_4595 0.50 -1.6
5,068,891 + Pf1N1B4_4595 0.64 +1.2
5,069,516 + -0.1
5,069,785 + Pf1N1B4_4596 0.36 -0.8
5,069,785 + +0.2
5,069,917 + Pf1N1B4_4596 0.53 -0.7
5,069,917 + +0.3
5,069,917 + Pf1N1B4_4596 0.53 +1.1
5,070,046 + Pf1N1B4_4596 0.70 -2.1
5,070,046 + Pf1N1B4_4596 0.70 -2.6
5,070,047 - +0.7
5,070,047 - +0.3
5,070,222 + -0.6
5,070,222 + -1.4
5,070,223 - -0.2
5,070,235 + -1.7
5,070,235 + -1.0
5,070,235 + -0.1
5,070,235 + -0.1
5,070,235 + -2.6
5,070,235 + +0.4
5,070,235 + -1.7
5,070,236 - +1.2
5,070,270 + +1.1
5,070,974 + Pf1N1B4_4597 0.83 -1.7
5,070,978 + +0.3
5,071,003 - -0.4
5,071,097 + +0.1
5,071,100 + +2.3
5,071,101 - +2.6
5,071,109 + -0.4
5,071,205 + +0.9

Or see this region's nucleotide sequence