Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4407

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4405 and Pf1N1B4_4406 overlap by 31 nucleotidesPf1N1B4_4406 and Pf1N1B4_4407 overlap by 144 nucleotidesPf1N1B4_4407 and Pf1N1B4_4408 are separated by 917 nucleotides Pf1N1B4_4405 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 4,865,886 to 4,867,397 _4405 Pf1N1B4_4406 - hypothetical protein, at 4,867,367 to 4,867,585 _4406 Pf1N1B4_4407 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 4,867,442 to 4,871,356 _4407 Pf1N1B4_4408 - two-component response regulator, at 4,872,274 to 4,872,879 _4408 Position (kb) 4867 4868 4869 4870 4871 4872Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4866.773 kb on + strand, within Pf1N1B4_4405at 4866.773 kb on + strand, within Pf1N1B4_4405at 4866.774 kb on - strand, within Pf1N1B4_4405at 4867.039 kb on + strand, within Pf1N1B4_4405at 4867.189 kb on + strand, within Pf1N1B4_4405at 4867.189 kb on + strand, within Pf1N1B4_4405at 4867.189 kb on + strand, within Pf1N1B4_4405at 4867.190 kb on - strand, within Pf1N1B4_4405at 4867.704 kb on + strandat 4867.878 kb on - strand, within Pf1N1B4_4407at 4868.062 kb on - strand, within Pf1N1B4_4407at 4868.094 kb on - strand, within Pf1N1B4_4407at 4868.112 kb on - strand, within Pf1N1B4_4407at 4868.426 kb on + strand, within Pf1N1B4_4407at 4868.427 kb on - strand, within Pf1N1B4_4407at 4868.434 kb on + strand, within Pf1N1B4_4407at 4868.435 kb on - strand, within Pf1N1B4_4407at 4868.643 kb on - strand, within Pf1N1B4_4407at 4868.685 kb on - strand, within Pf1N1B4_4407at 4868.765 kb on + strand, within Pf1N1B4_4407at 4868.766 kb on - strandat 4868.873 kb on + strand, within Pf1N1B4_4407at 4869.176 kb on + strandat 4869.176 kb on + strand, within Pf1N1B4_4407at 4869.177 kb on - strand, within Pf1N1B4_4407at 4869.246 kb on - strand, within Pf1N1B4_4407at 4869.246 kb on - strand, within Pf1N1B4_4407at 4869.272 kb on + strand, within Pf1N1B4_4407at 4869.278 kb on + strand, within Pf1N1B4_4407at 4869.279 kb on - strandat 4869.279 kb on - strand, within Pf1N1B4_4407at 4869.560 kb on + strand, within Pf1N1B4_4407at 4869.561 kb on - strand, within Pf1N1B4_4407at 4869.561 kb on - strand, within Pf1N1B4_4407at 4869.597 kb on - strand, within Pf1N1B4_4407at 4869.621 kb on - strand, within Pf1N1B4_4407at 4869.757 kb on - strand, within Pf1N1B4_4407at 4869.974 kb on + strand, within Pf1N1B4_4407at 4869.974 kb on + strand, within Pf1N1B4_4407at 4870.049 kb on + strand, within Pf1N1B4_4407at 4870.051 kb on + strand, within Pf1N1B4_4407at 4870.051 kb on + strand, within Pf1N1B4_4407at 4870.052 kb on - strand, within Pf1N1B4_4407at 4870.052 kb on - strand, within Pf1N1B4_4407at 4870.053 kb on + strand, within Pf1N1B4_4407at 4870.053 kb on + strand, within Pf1N1B4_4407at 4870.053 kb on + strandat 4870.053 kb on + strand, within Pf1N1B4_4407at 4870.053 kb on + strand, within Pf1N1B4_4407at 4870.054 kb on - strand, within Pf1N1B4_4407at 4870.054 kb on - strand, within Pf1N1B4_4407at 4870.054 kb on - strand, within Pf1N1B4_4407at 4870.054 kb on - strand, within Pf1N1B4_4407at 4870.054 kb on - strand, within Pf1N1B4_4407at 4870.054 kb on - strand, within Pf1N1B4_4407at 4870.054 kb on - strand, within Pf1N1B4_4407at 4870.054 kb on - strand, within Pf1N1B4_4407at 4870.054 kb on - strand, within Pf1N1B4_4407at 4870.054 kb on - strand, within Pf1N1B4_4407at 4870.054 kb on - strand, within Pf1N1B4_4407at 4870.204 kb on + strand, within Pf1N1B4_4407at 4870.229 kb on + strand, within Pf1N1B4_4407at 4870.490 kb on + strand, within Pf1N1B4_4407at 4870.809 kb on + strand, within Pf1N1B4_4407at 4870.810 kb on - strand, within Pf1N1B4_4407at 4870.851 kb on + strand, within Pf1N1B4_4407at 4871.125 kb on - strandat 4871.214 kb on - strandat 4871.478 kb on + strandat 4871.961 kb on + strandat 4872.344 kb on + strand, within Pf1N1B4_4408

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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4,866,773 + Pf1N1B4_4405 0.59 -1.2
4,866,773 + Pf1N1B4_4405 0.59 -0.5
4,866,774 - Pf1N1B4_4405 0.59 +0.8
4,867,039 + Pf1N1B4_4405 0.76 -0.3
4,867,189 + Pf1N1B4_4405 0.86 -0.3
4,867,189 + Pf1N1B4_4405 0.86 +1.6
4,867,189 + Pf1N1B4_4405 0.86 +0.0
4,867,190 - Pf1N1B4_4405 0.86 -1.3
4,867,704 + +0.4
4,867,878 - Pf1N1B4_4407 0.11 -0.9
4,868,062 - Pf1N1B4_4407 0.16 +1.1
4,868,094 - Pf1N1B4_4407 0.17 -0.0
4,868,112 - Pf1N1B4_4407 0.17 +1.3
4,868,426 + Pf1N1B4_4407 0.25 -1.2
4,868,427 - Pf1N1B4_4407 0.25 -0.2
4,868,434 + Pf1N1B4_4407 0.25 +1.3
4,868,435 - Pf1N1B4_4407 0.25 +1.7
4,868,643 - Pf1N1B4_4407 0.31 +2.0
4,868,685 - Pf1N1B4_4407 0.32 -0.0
4,868,765 + Pf1N1B4_4407 0.34 -1.9
4,868,766 - +2.4
4,868,873 + Pf1N1B4_4407 0.37 -1.2
4,869,176 + -1.3
4,869,176 + Pf1N1B4_4407 0.44 -2.5
4,869,177 - Pf1N1B4_4407 0.44 +0.3
4,869,246 - Pf1N1B4_4407 0.46 +0.0
4,869,246 - Pf1N1B4_4407 0.46 -1.7
4,869,272 + Pf1N1B4_4407 0.47 -0.9
4,869,278 + Pf1N1B4_4407 0.47 -1.1
4,869,279 - -1.5
4,869,279 - Pf1N1B4_4407 0.47 -2.1
4,869,560 + Pf1N1B4_4407 0.54 +0.4
4,869,561 - Pf1N1B4_4407 0.54 +0.7
4,869,561 - Pf1N1B4_4407 0.54 -0.1
4,869,597 - Pf1N1B4_4407 0.55 -1.9
4,869,621 - Pf1N1B4_4407 0.56 +0.5
4,869,757 - Pf1N1B4_4407 0.59 -2.2
4,869,974 + Pf1N1B4_4407 0.65 -0.7
4,869,974 + Pf1N1B4_4407 0.65 -3.0
4,870,049 + Pf1N1B4_4407 0.67 +0.5
4,870,051 + Pf1N1B4_4407 0.67 -3.3
4,870,051 + Pf1N1B4_4407 0.67 -0.7
4,870,052 - Pf1N1B4_4407 0.67 +0.8
4,870,052 - Pf1N1B4_4407 0.67 -0.5
4,870,053 + Pf1N1B4_4407 0.67 -0.5
4,870,053 + Pf1N1B4_4407 0.67 +0.5
4,870,053 + -0.5
4,870,053 + Pf1N1B4_4407 0.67 -2.1
4,870,053 + Pf1N1B4_4407 0.67 -0.5
4,870,054 - Pf1N1B4_4407 0.67 +0.6
4,870,054 - Pf1N1B4_4407 0.67 +2.1
4,870,054 - Pf1N1B4_4407 0.67 +0.0
4,870,054 - Pf1N1B4_4407 0.67 +1.1
4,870,054 - Pf1N1B4_4407 0.67 -1.5
4,870,054 - Pf1N1B4_4407 0.67 +1.2
4,870,054 - Pf1N1B4_4407 0.67 +0.3
4,870,054 - Pf1N1B4_4407 0.67 +0.4
4,870,054 - Pf1N1B4_4407 0.67 -0.1
4,870,054 - Pf1N1B4_4407 0.67 +0.9
4,870,054 - Pf1N1B4_4407 0.67 -0.9
4,870,204 + Pf1N1B4_4407 0.71 -0.5
4,870,229 + Pf1N1B4_4407 0.71 +0.3
4,870,490 + Pf1N1B4_4407 0.78 -0.6
4,870,809 + Pf1N1B4_4407 0.86 +0.9
4,870,810 - Pf1N1B4_4407 0.86 +1.9
4,870,851 + Pf1N1B4_4407 0.87 -0.1
4,871,125 - +0.6
4,871,214 - +0.2
4,871,478 + +0.3
4,871,961 + -1.3
4,872,344 + Pf1N1B4_4408 0.12 -0.3

Or see this region's nucleotide sequence