Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4404 and Pf1N1B4_4405 are separated by 227 nucleotides Pf1N1B4_4405 and Pf1N1B4_4406 overlap by 31 nucleotides Pf1N1B4_4406 and Pf1N1B4_4407 overlap by 144 nucleotides
Pf1N1B4_4404 - Transcriptional regulator, LysR family, at 4,864,693 to 4,865,658
_4404
Pf1N1B4_4405 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 4,865,886 to 4,867,397
_4405
Pf1N1B4_4406 - hypothetical protein, at 4,867,367 to 4,867,585
_4406
Pf1N1B4_4407 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 4,867,442 to 4,871,356
_4407
Position (kb)
4865
4866
4867
4868 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4865.056 kb on + strand, within Pf1N1B4_4404 at 4865.249 kb on + strand, within Pf1N1B4_4404 at 4865.249 kb on + strand, within Pf1N1B4_4404 at 4865.249 kb on + strand, within Pf1N1B4_4404 at 4865.442 kb on + strand, within Pf1N1B4_4404 at 4865.464 kb on + strand, within Pf1N1B4_4404 at 4865.465 kb on - strand at 4865.667 kb on + strand at 4865.754 kb on + strand at 4865.754 kb on + strand at 4865.775 kb on + strand at 4865.775 kb on + strand at 4865.775 kb on + strand at 4865.775 kb on + strand at 4865.775 kb on + strand at 4865.775 kb on + strand at 4865.854 kb on + strand at 4865.855 kb on - strand at 4865.855 kb on - strand at 4865.855 kb on - strand at 4865.858 kb on + strand at 4865.858 kb on + strand at 4865.858 kb on + strand at 4865.859 kb on - strand at 4865.859 kb on - strand at 4865.859 kb on - strand at 4865.859 kb on - strand at 4865.859 kb on - strand at 4865.859 kb on - strand at 4865.893 kb on + strand at 4865.893 kb on + strand at 4865.894 kb on - strand at 4865.896 kb on + strand at 4865.916 kb on - strand at 4866.102 kb on + strand, within Pf1N1B4_4405 at 4866.103 kb on - strand, within Pf1N1B4_4405 at 4866.103 kb on - strand at 4866.103 kb on - strand, within Pf1N1B4_4405 at 4866.245 kb on + strand, within Pf1N1B4_4405 at 4866.245 kb on + strand, within Pf1N1B4_4405 at 4866.296 kb on + strand, within Pf1N1B4_4405 at 4866.369 kb on + strand, within Pf1N1B4_4405 at 4866.369 kb on + strand, within Pf1N1B4_4405 at 4866.370 kb on - strand at 4866.371 kb on + strand, within Pf1N1B4_4405 at 4866.773 kb on + strand, within Pf1N1B4_4405 at 4866.773 kb on + strand, within Pf1N1B4_4405 at 4866.774 kb on - strand, within Pf1N1B4_4405 at 4867.039 kb on + strand, within Pf1N1B4_4405 at 4867.189 kb on + strand, within Pf1N1B4_4405 at 4867.189 kb on + strand, within Pf1N1B4_4405 at 4867.189 kb on + strand, within Pf1N1B4_4405 at 4867.190 kb on - strand, within Pf1N1B4_4405 at 4867.704 kb on + strand at 4867.878 kb on - strand, within Pf1N1B4_4407 at 4868.062 kb on - strand, within Pf1N1B4_4407 at 4868.094 kb on - strand, within Pf1N1B4_4407 at 4868.112 kb on - strand, within Pf1N1B4_4407
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 4,865,056 + Pf1N1B4_4404 0.38 -2.6 4,865,249 + Pf1N1B4_4404 0.58 +0.5 4,865,249 + Pf1N1B4_4404 0.58 +0.2 4,865,249 + Pf1N1B4_4404 0.58 +1.6 4,865,442 + Pf1N1B4_4404 0.78 -1.7 4,865,464 + Pf1N1B4_4404 0.80 +0.1 4,865,465 - +2.0 4,865,667 + -1.6 4,865,754 + -0.7 4,865,754 + -1.2 4,865,775 + -0.7 4,865,775 + -0.2 4,865,775 + +0.5 4,865,775 + +0.6 4,865,775 + +1.8 4,865,775 + -1.9 4,865,854 + +0.8 4,865,855 - +1.4 4,865,855 - -0.4 4,865,855 - +0.5 4,865,858 + +0.4 4,865,858 + -1.0 4,865,858 + -1.5 4,865,859 - +3.0 4,865,859 - +0.5 4,865,859 - -1.9 4,865,859 - +1.2 4,865,859 - +0.4 4,865,859 - -1.3 4,865,893 + -2.2 4,865,893 + +0.3 4,865,894 - -0.3 4,865,896 + +0.3 4,865,916 - +0.1 4,866,102 + Pf1N1B4_4405 0.14 -1.3 4,866,103 - Pf1N1B4_4405 0.14 -0.8 4,866,103 - +1.3 4,866,103 - Pf1N1B4_4405 0.14 +2.4 4,866,245 + Pf1N1B4_4405 0.24 -0.6 4,866,245 + Pf1N1B4_4405 0.24 -1.9 4,866,296 + Pf1N1B4_4405 0.27 -2.7 4,866,369 + Pf1N1B4_4405 0.32 -0.9 4,866,369 + Pf1N1B4_4405 0.32 +3.8 4,866,370 - -1.0 4,866,371 + Pf1N1B4_4405 0.32 +1.3 4,866,773 + Pf1N1B4_4405 0.59 -1.2 4,866,773 + Pf1N1B4_4405 0.59 -0.5 4,866,774 - Pf1N1B4_4405 0.59 +0.8 4,867,039 + Pf1N1B4_4405 0.76 -0.3 4,867,189 + Pf1N1B4_4405 0.86 -0.3 4,867,189 + Pf1N1B4_4405 0.86 +1.6 4,867,189 + Pf1N1B4_4405 0.86 +0.0 4,867,190 - Pf1N1B4_4405 0.86 -1.3 4,867,704 + +0.4 4,867,878 - Pf1N1B4_4407 0.11 -0.9 4,868,062 - Pf1N1B4_4407 0.16 +1.1 4,868,094 - Pf1N1B4_4407 0.17 -0.0 4,868,112 - Pf1N1B4_4407 0.17 +1.3
Or see this region's nucleotide sequence