Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3771 and Pf1N1B4_3772 are separated by 195 nucleotides Pf1N1B4_3772 and Pf1N1B4_3773 are separated by 102 nucleotides Pf1N1B4_3773 and Pf1N1B4_3774 are separated by 77 nucleotides Pf1N1B4_3774 and Pf1N1B4_3775 are separated by 75 nucleotides
Pf1N1B4_3771 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 4,181,685 to 4,182,851
_3771
Pf1N1B4_3772 - Glutaredoxin, at 4,183,047 to 4,183,418
_3772
Pf1N1B4_3773 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 4,183,521 to 4,184,066
_3773
Pf1N1B4_3774 - Smr domain protein, at 4,184,144 to 4,184,701
_3774
Pf1N1B4_3775 - FIG00956876: hypothetical protein, at 4,184,777 to 4,185,097
_3775
Position (kb)
4183
4184
4185 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4182.869 kb on + strand at 4182.892 kb on - strand at 4182.982 kb on + strand at 4182.983 kb on - strand at 4182.983 kb on - strand at 4182.987 kb on + strand at 4182.988 kb on - strand at 4182.988 kb on - strand at 4182.988 kb on - strand at 4182.988 kb on - strand at 4183.099 kb on + strand, within Pf1N1B4_3772 at 4183.099 kb on + strand, within Pf1N1B4_3772 at 4183.099 kb on + strand, within Pf1N1B4_3772 at 4183.099 kb on + strand, within Pf1N1B4_3772 at 4183.100 kb on - strand, within Pf1N1B4_3772 at 4183.100 kb on - strand, within Pf1N1B4_3772 at 4183.100 kb on - strand, within Pf1N1B4_3772 at 4183.100 kb on - strand at 4183.100 kb on - strand, within Pf1N1B4_3772 at 4183.100 kb on - strand, within Pf1N1B4_3772 at 4183.100 kb on - strand, within Pf1N1B4_3772 at 4183.100 kb on - strand, within Pf1N1B4_3772 at 4183.100 kb on - strand, within Pf1N1B4_3772 at 4183.100 kb on - strand, within Pf1N1B4_3772 at 4183.100 kb on - strand, within Pf1N1B4_3772 at 4183.282 kb on + strand, within Pf1N1B4_3772 at 4183.421 kb on + strand at 4183.421 kb on + strand at 4183.421 kb on + strand at 4183.421 kb on + strand at 4183.421 kb on + strand at 4183.421 kb on + strand at 4183.421 kb on + strand at 4183.421 kb on + strand at 4183.421 kb on + strand at 4183.421 kb on + strand at 4183.421 kb on + strand at 4183.422 kb on - strand at 4183.422 kb on - strand at 4183.422 kb on - strand at 4183.422 kb on - strand at 4183.422 kb on - strand at 4183.422 kb on - strand at 4183.422 kb on - strand at 4183.422 kb on - strand at 4183.423 kb on + strand at 4183.423 kb on + strand at 4183.423 kb on + strand at 4183.458 kb on - strand at 4183.459 kb on - strand at 4183.522 kb on + strand at 4184.187 kb on + strand at 4184.331 kb on - strand, within Pf1N1B4_3774 at 4184.331 kb on - strand, within Pf1N1B4_3774 at 4184.872 kb on - strand, within Pf1N1B4_3775 at 4184.872 kb on - strand, within Pf1N1B4_3775 at 4184.872 kb on - strand, within Pf1N1B4_3775
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 4,182,869 + +0.1 4,182,892 - -1.8 4,182,982 + +2.5 4,182,983 - -1.3 4,182,983 - +2.7 4,182,987 + +1.0 4,182,988 - -1.5 4,182,988 - +0.7 4,182,988 - -2.6 4,182,988 - +1.3 4,183,099 + Pf1N1B4_3772 0.14 -1.5 4,183,099 + Pf1N1B4_3772 0.14 +1.5 4,183,099 + Pf1N1B4_3772 0.14 +1.8 4,183,099 + Pf1N1B4_3772 0.14 -1.3 4,183,100 - Pf1N1B4_3772 0.14 +1.1 4,183,100 - Pf1N1B4_3772 0.14 +2.1 4,183,100 - Pf1N1B4_3772 0.14 +0.1 4,183,100 - +2.8 4,183,100 - Pf1N1B4_3772 0.14 +1.2 4,183,100 - Pf1N1B4_3772 0.14 -0.4 4,183,100 - Pf1N1B4_3772 0.14 -1.8 4,183,100 - Pf1N1B4_3772 0.14 +0.5 4,183,100 - Pf1N1B4_3772 0.14 +0.8 4,183,100 - Pf1N1B4_3772 0.14 -0.9 4,183,100 - Pf1N1B4_3772 0.14 +1.2 4,183,282 + Pf1N1B4_3772 0.63 +0.9 4,183,421 + -0.5 4,183,421 + -1.2 4,183,421 + +0.7 4,183,421 + -2.2 4,183,421 + +1.3 4,183,421 + -0.5 4,183,421 + -2.3 4,183,421 + -0.3 4,183,421 + -1.1 4,183,421 + +0.2 4,183,421 + -0.9 4,183,422 - +0.9 4,183,422 - -1.1 4,183,422 - -0.3 4,183,422 - -1.4 4,183,422 - +1.2 4,183,422 - +1.0 4,183,422 - +2.2 4,183,422 - -0.8 4,183,423 + -1.6 4,183,423 + +0.5 4,183,423 + -1.5 4,183,458 - +0.2 4,183,459 - +1.4 4,183,522 + +1.5 4,184,187 + +0.5 4,184,331 - Pf1N1B4_3774 0.34 -1.0 4,184,331 - Pf1N1B4_3774 0.34 -1.3 4,184,872 - Pf1N1B4_3775 0.30 +2.7 4,184,872 - Pf1N1B4_3775 0.30 -0.4 4,184,872 - Pf1N1B4_3775 0.30 +0.9
Or see this region's nucleotide sequence