Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3576 and Pf1N1B4_3577 are separated by 280 nucleotides Pf1N1B4_3577 and Pf1N1B4_3578 overlap by 19 nucleotides
Pf1N1B4_3576 - Alkaline phosphatase (EC 3.1.3.1), at 3,984,133 to 3,987,861
_3576
Pf1N1B4_3577 - Glucose-1-phosphate cytidylyltransferase (EC 2.7.7.33), at 3,988,142 to 3,988,915
_3577
Pf1N1B4_3578 - Similar to CDP-glucose 4,6-dehydratase (EC 4.2.1.45), at 3,988,897 to 3,989,979
_3578
Position (kb)
3988
3989 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3987.205 kb on + strand, within Pf1N1B4_3576 at 3987.206 kb on - strand, within Pf1N1B4_3576 at 3987.206 kb on - strand, within Pf1N1B4_3576 at 3987.238 kb on + strand, within Pf1N1B4_3576 at 3987.238 kb on + strand, within Pf1N1B4_3576 at 3987.238 kb on + strand, within Pf1N1B4_3576 at 3987.238 kb on + strand, within Pf1N1B4_3576 at 3987.238 kb on + strand, within Pf1N1B4_3576 at 3987.238 kb on + strand at 3987.238 kb on + strand, within Pf1N1B4_3576 at 3987.238 kb on + strand, within Pf1N1B4_3576 at 3987.238 kb on + strand, within Pf1N1B4_3576 at 3987.239 kb on - strand, within Pf1N1B4_3576 at 3987.239 kb on - strand, within Pf1N1B4_3576 at 3987.259 kb on + strand, within Pf1N1B4_3576 at 3987.340 kb on + strand, within Pf1N1B4_3576 at 3987.340 kb on + strand, within Pf1N1B4_3576 at 3987.340 kb on + strand at 3987.340 kb on + strand at 3987.340 kb on + strand, within Pf1N1B4_3576 at 3987.340 kb on + strand, within Pf1N1B4_3576 at 3987.340 kb on + strand, within Pf1N1B4_3576 at 3987.340 kb on + strand, within Pf1N1B4_3576 at 3987.341 kb on - strand at 3987.400 kb on + strand, within Pf1N1B4_3576 at 3987.401 kb on - strand, within Pf1N1B4_3576 at 3987.401 kb on - strand, within Pf1N1B4_3576 at 3987.574 kb on - strand at 3987.574 kb on - strand at 3987.577 kb on + strand at 3987.577 kb on + strand at 3987.577 kb on + strand at 3987.577 kb on + strand at 3987.577 kb on + strand at 3987.577 kb on + strand at 3987.577 kb on + strand at 3987.577 kb on + strand at 3987.577 kb on + strand at 3987.578 kb on - strand at 3987.578 kb on - strand at 3987.652 kb on + strand at 3987.652 kb on + strand at 3987.652 kb on + strand at 3987.652 kb on + strand at 3987.700 kb on + strand at 3987.700 kb on + strand at 3987.700 kb on + strand at 3987.700 kb on + strand at 3987.721 kb on - strand at 3987.750 kb on - strand at 3987.971 kb on + strand at 3987.972 kb on - strand at 3987.972 kb on - strand at 3988.016 kb on - strand at 3988.024 kb on - strand at 3988.103 kb on + strand at 3988.104 kb on - strand at 3988.153 kb on - strand at 3988.263 kb on - strand, within Pf1N1B4_3577 at 3988.305 kb on - strand, within Pf1N1B4_3577 at 3988.305 kb on - strand, within Pf1N1B4_3577 at 3988.340 kb on + strand, within Pf1N1B4_3577 at 3988.341 kb on - strand, within Pf1N1B4_3577 at 3988.341 kb on - strand, within Pf1N1B4_3577 at 3988.395 kb on + strand, within Pf1N1B4_3577 at 3988.396 kb on - strand, within Pf1N1B4_3577 at 3988.396 kb on - strand, within Pf1N1B4_3577 at 3988.396 kb on - strand, within Pf1N1B4_3577 at 3988.396 kb on - strand, within Pf1N1B4_3577 at 3988.407 kb on - strand at 3988.407 kb on - strand, within Pf1N1B4_3577 at 3988.407 kb on - strand, within Pf1N1B4_3577 at 3988.520 kb on + strand, within Pf1N1B4_3577 at 3988.521 kb on - strand, within Pf1N1B4_3577 at 3988.565 kb on + strand, within Pf1N1B4_3577 at 3988.736 kb on + strand, within Pf1N1B4_3577 at 3988.736 kb on + strand, within Pf1N1B4_3577 at 3988.737 kb on - strand at 3988.737 kb on - strand, within Pf1N1B4_3577 at 3989.124 kb on - strand, within Pf1N1B4_3578 at 3989.219 kb on - strand, within Pf1N1B4_3578 at 3989.378 kb on - strand, within Pf1N1B4_3578 at 3989.420 kb on - strand, within Pf1N1B4_3578 at 3989.450 kb on - strand, within Pf1N1B4_3578 at 3989.576 kb on + strand, within Pf1N1B4_3578 at 3989.577 kb on - strand, within Pf1N1B4_3578 at 3989.595 kb on - strand, within Pf1N1B4_3578
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,987,205 + Pf1N1B4_3576 0.82 +0.7 3,987,206 - Pf1N1B4_3576 0.82 -0.3 3,987,206 - Pf1N1B4_3576 0.82 -1.2 3,987,238 + Pf1N1B4_3576 0.83 -0.0 3,987,238 + Pf1N1B4_3576 0.83 +1.0 3,987,238 + Pf1N1B4_3576 0.83 +0.6 3,987,238 + Pf1N1B4_3576 0.83 +1.2 3,987,238 + Pf1N1B4_3576 0.83 +1.7 3,987,238 + -0.7 3,987,238 + Pf1N1B4_3576 0.83 +1.6 3,987,238 + Pf1N1B4_3576 0.83 -0.7 3,987,238 + Pf1N1B4_3576 0.83 +2.1 3,987,239 - Pf1N1B4_3576 0.83 -2.0 3,987,239 - Pf1N1B4_3576 0.83 -1.1 3,987,259 + Pf1N1B4_3576 0.84 -1.7 3,987,340 + Pf1N1B4_3576 0.86 -0.0 3,987,340 + Pf1N1B4_3576 0.86 -0.8 3,987,340 + -0.5 3,987,340 + +1.8 3,987,340 + Pf1N1B4_3576 0.86 +1.0 3,987,340 + Pf1N1B4_3576 0.86 +1.1 3,987,340 + Pf1N1B4_3576 0.86 +0.2 3,987,340 + Pf1N1B4_3576 0.86 -1.0 3,987,341 - +0.1 3,987,400 + Pf1N1B4_3576 0.88 +0.4 3,987,401 - Pf1N1B4_3576 0.88 +0.7 3,987,401 - Pf1N1B4_3576 0.88 +0.1 3,987,574 - +0.3 3,987,574 - -0.8 3,987,577 + -0.1 3,987,577 + -0.9 3,987,577 + -1.9 3,987,577 + -0.8 3,987,577 + +1.6 3,987,577 + +0.7 3,987,577 + -1.6 3,987,577 + +1.1 3,987,577 + +1.6 3,987,578 - -1.7 3,987,578 - +1.2 3,987,652 + -1.5 3,987,652 + -0.3 3,987,652 + -1.3 3,987,652 + -0.4 3,987,700 + -1.1 3,987,700 + -0.4 3,987,700 + +0.6 3,987,700 + -2.1 3,987,721 - +2.4 3,987,750 - -2.0 3,987,971 + +1.4 3,987,972 - +3.1 3,987,972 - -0.5 3,988,016 - +1.1 3,988,024 - +2.6 3,988,103 + -0.1 3,988,104 - -0.7 3,988,153 - +1.1 3,988,263 - Pf1N1B4_3577 0.16 -1.8 3,988,305 - Pf1N1B4_3577 0.21 -0.5 3,988,305 - Pf1N1B4_3577 0.21 -0.4 3,988,340 + Pf1N1B4_3577 0.26 -1.5 3,988,341 - Pf1N1B4_3577 0.26 +0.7 3,988,341 - Pf1N1B4_3577 0.26 +0.1 3,988,395 + Pf1N1B4_3577 0.33 -0.1 3,988,396 - Pf1N1B4_3577 0.33 +1.4 3,988,396 - Pf1N1B4_3577 0.33 +1.0 3,988,396 - Pf1N1B4_3577 0.33 -1.7 3,988,396 - Pf1N1B4_3577 0.33 +0.7 3,988,407 - +1.3 3,988,407 - Pf1N1B4_3577 0.34 +0.4 3,988,407 - Pf1N1B4_3577 0.34 -2.0 3,988,520 + Pf1N1B4_3577 0.49 -1.4 3,988,521 - Pf1N1B4_3577 0.49 -0.2 3,988,565 + Pf1N1B4_3577 0.55 -0.0 3,988,736 + Pf1N1B4_3577 0.77 +1.8 3,988,736 + Pf1N1B4_3577 0.77 -1.2 3,988,737 - +0.6 3,988,737 - Pf1N1B4_3577 0.77 -1.3 3,989,124 - Pf1N1B4_3578 0.21 -0.2 3,989,219 - Pf1N1B4_3578 0.30 +0.5 3,989,378 - Pf1N1B4_3578 0.44 -0.2 3,989,420 - Pf1N1B4_3578 0.48 +1.1 3,989,450 - Pf1N1B4_3578 0.51 +0.2 3,989,576 + Pf1N1B4_3578 0.63 -0.3 3,989,577 - Pf1N1B4_3578 0.63 -2.5 3,989,595 - Pf1N1B4_3578 0.64 +0.2
Or see this region's nucleotide sequence