Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3486 and Pf1N1B4_3487 are separated by 340 nucleotides Pf1N1B4_3487 and Pf1N1B4_3488 are separated by 145 nucleotides
Pf1N1B4_3486 - Transcriptional regulator, AraC family, at 3,893,011 to 3,894,024
_3486
Pf1N1B4_3487 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, aspartate, at 3,894,365 to 3,895,699
_3487
Pf1N1B4_3488 - Alpha-methylacyl-CoA racemase (EC 5.1.99.4), at 3,895,845 to 3,897,026
_3488
Position (kb)
3894
3895
3896 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3893.399 kb on - strand, within Pf1N1B4_3486 at 3893.501 kb on + strand, within Pf1N1B4_3486 at 3893.718 kb on - strand, within Pf1N1B4_3486 at 3893.828 kb on + strand, within Pf1N1B4_3486 at 3893.828 kb on + strand, within Pf1N1B4_3486 at 3893.829 kb on - strand, within Pf1N1B4_3486 at 3893.829 kb on - strand, within Pf1N1B4_3486 at 3893.829 kb on - strand, within Pf1N1B4_3486 at 3894.024 kb on + strand at 3894.025 kb on - strand at 3894.177 kb on + strand at 3894.338 kb on + strand at 3894.338 kb on + strand at 3894.362 kb on - strand at 3894.362 kb on - strand at 3894.362 kb on - strand at 3894.386 kb on + strand at 3894.387 kb on - strand at 3894.388 kb on + strand at 3894.389 kb on - strand at 3894.399 kb on - strand at 3894.536 kb on - strand, within Pf1N1B4_3487 at 3894.608 kb on + strand at 3894.609 kb on - strand, within Pf1N1B4_3487 at 3894.609 kb on - strand, within Pf1N1B4_3487 at 3895.001 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.050 kb on - strand at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.133 kb on + strand at 3895.283 kb on - strand, within Pf1N1B4_3487 at 3895.283 kb on - strand, within Pf1N1B4_3487 at 3895.292 kb on + strand, within Pf1N1B4_3487 at 3895.292 kb on + strand, within Pf1N1B4_3487 at 3895.293 kb on - strand, within Pf1N1B4_3487 at 3895.293 kb on - strand, within Pf1N1B4_3487 at 3895.333 kb on + strand, within Pf1N1B4_3487 at 3895.334 kb on - strand, within Pf1N1B4_3487 at 3895.334 kb on - strand, within Pf1N1B4_3487 at 3895.650 kb on + strand at 3895.651 kb on - strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.701 kb on + strand at 3895.702 kb on - strand at 3895.702 kb on - strand at 3895.814 kb on + strand at 3895.844 kb on + strand at 3895.844 kb on + strand at 3895.844 kb on + strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3896.025 kb on + strand at 3896.025 kb on + strand, within Pf1N1B4_3488 at 3896.025 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.238 kb on + strand, within Pf1N1B4_3488 at 3896.238 kb on + strand, within Pf1N1B4_3488 at 3896.239 kb on - strand, within Pf1N1B4_3488 at 3896.239 kb on - strand, within Pf1N1B4_3488 at 3896.268 kb on + strand, within Pf1N1B4_3488 at 3896.269 kb on - strand, within Pf1N1B4_3488 at 3896.269 kb on - strand, within Pf1N1B4_3488 at 3896.295 kb on + strand, within Pf1N1B4_3488 at 3896.296 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.553 kb on + strand, within Pf1N1B4_3488 at 3896.553 kb on + strand, within Pf1N1B4_3488 at 3896.553 kb on + strand at 3896.554 kb on - strand, within Pf1N1B4_3488 at 3896.556 kb on + strand, within Pf1N1B4_3488 at 3896.556 kb on + strand, within Pf1N1B4_3488
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,893,399 - Pf1N1B4_3486 0.38 -1.0 3,893,501 + Pf1N1B4_3486 0.48 -0.2 3,893,718 - Pf1N1B4_3486 0.70 -1.4 3,893,828 + Pf1N1B4_3486 0.81 -0.4 3,893,828 + Pf1N1B4_3486 0.81 -2.9 3,893,829 - Pf1N1B4_3486 0.81 +0.2 3,893,829 - Pf1N1B4_3486 0.81 -1.6 3,893,829 - Pf1N1B4_3486 0.81 +0.1 3,894,024 + -1.0 3,894,025 - +0.5 3,894,177 + -1.1 3,894,338 + +0.0 3,894,338 + -2.6 3,894,362 - +0.5 3,894,362 - +0.2 3,894,362 - +1.1 3,894,386 + -2.2 3,894,387 - +2.5 3,894,388 + -1.6 3,894,389 - -0.5 3,894,399 - +0.8 3,894,536 - Pf1N1B4_3487 0.13 -0.7 3,894,608 + +3.1 3,894,609 - Pf1N1B4_3487 0.18 -0.4 3,894,609 - Pf1N1B4_3487 0.18 +1.5 3,895,001 + Pf1N1B4_3487 0.48 -1.7 3,895,049 + Pf1N1B4_3487 0.51 +1.3 3,895,049 + Pf1N1B4_3487 0.51 +0.5 3,895,049 + Pf1N1B4_3487 0.51 +0.8 3,895,049 + Pf1N1B4_3487 0.51 +1.5 3,895,049 + Pf1N1B4_3487 0.51 +0.7 3,895,049 + Pf1N1B4_3487 0.51 +0.7 3,895,049 + Pf1N1B4_3487 0.51 +3.1 3,895,049 + Pf1N1B4_3487 0.51 +2.9 3,895,049 + Pf1N1B4_3487 0.51 -1.8 3,895,049 + Pf1N1B4_3487 0.51 +0.0 3,895,049 + Pf1N1B4_3487 0.51 +2.0 3,895,049 + Pf1N1B4_3487 0.51 +2.1 3,895,049 + Pf1N1B4_3487 0.51 +0.3 3,895,049 + Pf1N1B4_3487 0.51 -0.9 3,895,049 + Pf1N1B4_3487 0.51 +2.1 3,895,049 + Pf1N1B4_3487 0.51 +1.3 3,895,050 - -0.1 3,895,050 - Pf1N1B4_3487 0.51 -1.6 3,895,050 - Pf1N1B4_3487 0.51 +1.1 3,895,050 - Pf1N1B4_3487 0.51 +0.8 3,895,133 + -1.4 3,895,283 - Pf1N1B4_3487 0.69 +0.5 3,895,283 - Pf1N1B4_3487 0.69 +0.9 3,895,292 + Pf1N1B4_3487 0.69 +1.1 3,895,292 + Pf1N1B4_3487 0.69 +1.3 3,895,293 - Pf1N1B4_3487 0.70 -0.5 3,895,293 - Pf1N1B4_3487 0.70 -1.1 3,895,333 + Pf1N1B4_3487 0.73 -2.2 3,895,334 - Pf1N1B4_3487 0.73 +0.4 3,895,334 - Pf1N1B4_3487 0.73 -2.8 3,895,650 + -2.5 3,895,651 - +2.3 3,895,697 + -0.4 3,895,697 + +1.6 3,895,697 + +1.9 3,895,697 + -0.5 3,895,697 + -0.7 3,895,697 + +1.3 3,895,697 + +0.8 3,895,697 + -0.1 3,895,697 + -0.3 3,895,697 + -0.6 3,895,697 + +1.7 3,895,698 - +1.2 3,895,698 - -1.9 3,895,698 - -0.6 3,895,698 - +0.8 3,895,698 - -1.6 3,895,698 - +0.7 3,895,701 + +0.7 3,895,702 - +1.9 3,895,702 - +1.2 3,895,814 + -1.4 3,895,844 + +1.1 3,895,844 + +0.3 3,895,844 + -1.0 3,895,845 - +0.1 3,895,845 - +0.0 3,895,845 - +0.7 3,895,845 - -2.1 3,895,845 - +0.5 3,895,845 - +0.8 3,895,845 - -0.9 3,895,845 - +1.5 3,895,845 - -1.3 3,895,845 - +0.6 3,895,845 - +0.8 3,895,845 - +2.9 3,895,845 - -0.4 3,895,845 - -1.6 3,895,845 - +2.7 3,896,025 + -1.1 3,896,025 + Pf1N1B4_3488 0.15 -0.2 3,896,025 + Pf1N1B4_3488 0.15 -0.7 3,896,202 + Pf1N1B4_3488 0.30 +0.4 3,896,202 + Pf1N1B4_3488 0.30 -1.7 3,896,202 + Pf1N1B4_3488 0.30 -2.9 3,896,202 + Pf1N1B4_3488 0.30 -2.9 3,896,202 + Pf1N1B4_3488 0.30 -0.1 3,896,202 + -1.1 3,896,202 + Pf1N1B4_3488 0.30 -0.8 3,896,202 + Pf1N1B4_3488 0.30 -2.3 3,896,202 + Pf1N1B4_3488 0.30 -0.9 3,896,203 - Pf1N1B4_3488 0.30 +0.6 3,896,203 - Pf1N1B4_3488 0.30 +1.5 3,896,203 - Pf1N1B4_3488 0.30 +0.6 3,896,238 + Pf1N1B4_3488 0.33 -1.8 3,896,238 + Pf1N1B4_3488 0.33 +0.3 3,896,239 - Pf1N1B4_3488 0.33 -0.3 3,896,239 - Pf1N1B4_3488 0.33 +0.7 3,896,268 + Pf1N1B4_3488 0.36 -0.1 3,896,269 - Pf1N1B4_3488 0.36 -1.7 3,896,269 - Pf1N1B4_3488 0.36 +1.6 3,896,295 + Pf1N1B4_3488 0.38 +0.0 3,896,296 - Pf1N1B4_3488 0.38 +0.4 3,896,548 - Pf1N1B4_3488 0.59 -1.1 3,896,548 - Pf1N1B4_3488 0.59 -0.5 3,896,548 - Pf1N1B4_3488 0.59 -1.9 3,896,553 + Pf1N1B4_3488 0.60 -0.1 3,896,553 + Pf1N1B4_3488 0.60 -0.2 3,896,553 + -0.3 3,896,554 - Pf1N1B4_3488 0.60 -0.3 3,896,556 + Pf1N1B4_3488 0.60 +0.5 3,896,556 + Pf1N1B4_3488 0.60 +0.2
Or see this region's nucleotide sequence