Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3460 and Pf1N1B4_3461 are separated by 274 nucleotides Pf1N1B4_3461 and Pf1N1B4_3462 overlap by 15 nucleotides Pf1N1B4_3462 and Pf1N1B4_3463 overlap by 4 nucleotides Pf1N1B4_3463 and Pf1N1B4_3464 are separated by 181 nucleotides
Pf1N1B4_3460 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 3,863,390 to 3,867,526
_3460
Pf1N1B4_3461 - hypothetical protein, at 3,867,801 to 3,867,920
_3461
Pf1N1B4_3462 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 3,867,906 to 3,869,510
_3462
Pf1N1B4_3463 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 3,869,507 to 3,870,199
_3463
Pf1N1B4_3464 - glutamine synthetase family protein, at 3,870,381 to 3,871,724
_3464
Position (kb)
3867
3868
3869
3870 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3866.906 kb on - strand, within Pf1N1B4_3460 at 3866.912 kb on + strand, within Pf1N1B4_3460 at 3867.122 kb on + strand at 3867.122 kb on + strand at 3867.123 kb on - strand at 3867.123 kb on - strand at 3867.123 kb on - strand at 3867.243 kb on + strand at 3867.243 kb on + strand at 3867.243 kb on + strand at 3867.244 kb on - strand at 3867.244 kb on - strand at 3867.244 kb on - strand at 3867.244 kb on - strand at 3867.409 kb on + strand at 3867.409 kb on + strand at 3867.410 kb on - strand at 3867.410 kb on - strand at 3867.527 kb on + strand at 3867.527 kb on + strand at 3867.528 kb on - strand at 3867.528 kb on - strand at 3867.551 kb on - strand at 3867.551 kb on - strand at 3867.551 kb on - strand at 3867.575 kb on - strand at 3867.577 kb on - strand at 3867.577 kb on - strand at 3867.577 kb on - strand at 3867.628 kb on - strand at 3867.642 kb on + strand at 3867.642 kb on + strand at 3867.642 kb on + strand at 3867.653 kb on - strand at 3867.654 kb on + strand at 3867.654 kb on + strand at 3867.861 kb on + strand at 3867.862 kb on - strand, within Pf1N1B4_3461 at 3867.862 kb on - strand, within Pf1N1B4_3461 at 3867.885 kb on + strand, within Pf1N1B4_3461 at 3867.886 kb on - strand, within Pf1N1B4_3461 at 3868.233 kb on + strand, within Pf1N1B4_3462 at 3868.234 kb on - strand, within Pf1N1B4_3462 at 3868.295 kb on + strand, within Pf1N1B4_3462 at 3868.295 kb on + strand, within Pf1N1B4_3462 at 3868.295 kb on + strand, within Pf1N1B4_3462 at 3868.295 kb on + strand, within Pf1N1B4_3462 at 3868.296 kb on - strand, within Pf1N1B4_3462 at 3868.533 kb on - strand, within Pf1N1B4_3462 at 3868.566 kb on + strand at 3868.567 kb on - strand, within Pf1N1B4_3462 at 3868.567 kb on - strand, within Pf1N1B4_3462 at 3869.157 kb on + strand, within Pf1N1B4_3462 at 3869.157 kb on + strand, within Pf1N1B4_3462 at 3869.157 kb on + strand, within Pf1N1B4_3462 at 3869.157 kb on + strand, within Pf1N1B4_3462 at 3869.268 kb on + strand, within Pf1N1B4_3462 at 3869.268 kb on + strand, within Pf1N1B4_3462 at 3869.269 kb on - strand, within Pf1N1B4_3462 at 3869.269 kb on - strand, within Pf1N1B4_3462 at 3869.302 kb on + strand, within Pf1N1B4_3462 at 3869.303 kb on - strand, within Pf1N1B4_3462 at 3869.303 kb on - strand, within Pf1N1B4_3462 at 3869.303 kb on - strand, within Pf1N1B4_3462 at 3869.342 kb on + strand, within Pf1N1B4_3462 at 3869.714 kb on + strand, within Pf1N1B4_3463 at 3869.834 kb on + strand, within Pf1N1B4_3463 at 3869.834 kb on + strand, within Pf1N1B4_3463 at 3869.834 kb on + strand, within Pf1N1B4_3463 at 3869.834 kb on + strand, within Pf1N1B4_3463 at 3869.834 kb on + strand, within Pf1N1B4_3463 at 3869.835 kb on - strand, within Pf1N1B4_3463 at 3869.835 kb on - strand, within Pf1N1B4_3463 at 3869.835 kb on - strand, within Pf1N1B4_3463 at 3869.835 kb on - strand, within Pf1N1B4_3463 at 3869.835 kb on - strand, within Pf1N1B4_3463 at 3869.893 kb on - strand, within Pf1N1B4_3463 at 3870.038 kb on + strand, within Pf1N1B4_3463 at 3870.039 kb on - strand, within Pf1N1B4_3463 at 3870.086 kb on + strand, within Pf1N1B4_3463 at 3870.188 kb on + strand at 3870.188 kb on + strand at 3870.189 kb on - strand at 3870.189 kb on - strand at 3870.189 kb on - strand at 3870.189 kb on - strand at 3870.243 kb on + strand at 3870.330 kb on + strand at 3870.330 kb on + strand at 3870.331 kb on - strand at 3870.360 kb on + strand at 3870.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,866,906 - Pf1N1B4_3460 0.85 -0.6 3,866,912 + Pf1N1B4_3460 0.85 -1.7 3,867,122 + +1.3 3,867,122 + -0.7 3,867,123 - -1.0 3,867,123 - -2.7 3,867,123 - +0.8 3,867,243 + +1.6 3,867,243 + +0.3 3,867,243 + +1.1 3,867,244 - +1.1 3,867,244 - +1.6 3,867,244 - +2.4 3,867,244 - +0.9 3,867,409 + -0.1 3,867,409 + -1.4 3,867,410 - -0.2 3,867,410 - -3.6 3,867,527 + -0.8 3,867,527 + +0.5 3,867,528 - -0.7 3,867,528 - +0.2 3,867,551 - -1.7 3,867,551 - +1.6 3,867,551 - +1.9 3,867,575 - +1.8 3,867,577 - +0.9 3,867,577 - +2.0 3,867,577 - +0.4 3,867,628 - -2.8 3,867,642 + +2.1 3,867,642 + -1.5 3,867,642 + -2.4 3,867,653 - +0.9 3,867,654 + -1.4 3,867,654 + +1.3 3,867,861 + +1.5 3,867,862 - Pf1N1B4_3461 0.51 +0.2 3,867,862 - Pf1N1B4_3461 0.51 -0.0 3,867,885 + Pf1N1B4_3461 0.70 -1.8 3,867,886 - Pf1N1B4_3461 0.71 +2.5 3,868,233 + Pf1N1B4_3462 0.20 -1.6 3,868,234 - Pf1N1B4_3462 0.20 -1.3 3,868,295 + Pf1N1B4_3462 0.24 +0.8 3,868,295 + Pf1N1B4_3462 0.24 -0.1 3,868,295 + Pf1N1B4_3462 0.24 -1.5 3,868,295 + Pf1N1B4_3462 0.24 -0.1 3,868,296 - Pf1N1B4_3462 0.24 +1.2 3,868,533 - Pf1N1B4_3462 0.39 -3.9 3,868,566 + +2.6 3,868,567 - Pf1N1B4_3462 0.41 +1.1 3,868,567 - Pf1N1B4_3462 0.41 +1.5 3,869,157 + Pf1N1B4_3462 0.78 -0.9 3,869,157 + Pf1N1B4_3462 0.78 -2.5 3,869,157 + Pf1N1B4_3462 0.78 +0.7 3,869,157 + Pf1N1B4_3462 0.78 -1.6 3,869,268 + Pf1N1B4_3462 0.85 -2.1 3,869,268 + Pf1N1B4_3462 0.85 -0.6 3,869,269 - Pf1N1B4_3462 0.85 -1.2 3,869,269 - Pf1N1B4_3462 0.85 +0.3 3,869,302 + Pf1N1B4_3462 0.87 +0.5 3,869,303 - Pf1N1B4_3462 0.87 +0.0 3,869,303 - Pf1N1B4_3462 0.87 +2.2 3,869,303 - Pf1N1B4_3462 0.87 -0.8 3,869,342 + Pf1N1B4_3462 0.89 +0.8 3,869,714 + Pf1N1B4_3463 0.30 +0.4 3,869,834 + Pf1N1B4_3463 0.47 +0.7 3,869,834 + Pf1N1B4_3463 0.47 -2.4 3,869,834 + Pf1N1B4_3463 0.47 +1.0 3,869,834 + Pf1N1B4_3463 0.47 -1.7 3,869,834 + Pf1N1B4_3463 0.47 +0.1 3,869,835 - Pf1N1B4_3463 0.47 -2.3 3,869,835 - Pf1N1B4_3463 0.47 +0.1 3,869,835 - Pf1N1B4_3463 0.47 -1.6 3,869,835 - Pf1N1B4_3463 0.47 -0.5 3,869,835 - Pf1N1B4_3463 0.47 +0.4 3,869,893 - Pf1N1B4_3463 0.56 -3.6 3,870,038 + Pf1N1B4_3463 0.77 +0.5 3,870,039 - Pf1N1B4_3463 0.77 -1.4 3,870,086 + Pf1N1B4_3463 0.84 +1.0 3,870,188 + +0.2 3,870,188 + +0.7 3,870,189 - +0.7 3,870,189 - +0.5 3,870,189 - +1.6 3,870,189 - +0.3 3,870,243 + -1.9 3,870,330 + +1.0 3,870,330 + -0.6 3,870,331 - +0.4 3,870,360 + +0.9 3,870,361 - -2.4
Or see this region's nucleotide sequence