Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_30

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_28 and Pf1N1B4_29 overlap by 4 nucleotidesPf1N1B4_29 and Pf1N1B4_5164 are separated by 225 nucleotidesPf1N1B4_5164 and Pf1N1B4_30 are separated by 102 nucleotidesPf1N1B4_30 and Pf1N1B4_31 overlap by 4 nucleotides Pf1N1B4_28 - FIG00958186: hypothetical protein, at 31,985 to 32,185 _28 Pf1N1B4_29 - FIG00967200: hypothetical protein, at 32,182 to 32,412 _29 Pf1N1B4_5164 - tRNA-Asn-GTT, at 32,638 to 32,710 _5164 Pf1N1B4_30 - Cation efflux system protein, at 32,813 to 35,899 _30 Pf1N1B4_31 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 35,896 to 37,017 _31 Position (kb) 32 33 34 35 36Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 31.892 kb on - strandat 31.952 kb on + strandat 31.952 kb on + strandat 31.958 kb on + strandat 31.958 kb on + strandat 31.959 kb on - strandat 31.959 kb on - strandat 31.959 kb on - strandat 31.959 kb on - strandat 31.959 kb on - strandat 32.001 kb on + strandat 32.002 kb on - strandat 32.144 kb on + strand, within Pf1N1B4_28at 32.170 kb on + strandat 32.171 kb on - strandat 32.171 kb on - strandat 32.223 kb on - strand, within Pf1N1B4_29at 32.223 kb on - strand, within Pf1N1B4_29at 32.232 kb on - strand, within Pf1N1B4_29at 32.232 kb on - strand, within Pf1N1B4_29at 32.232 kb on - strand, within Pf1N1B4_29at 32.463 kb on + strandat 32.517 kb on + strandat 32.526 kb on - strandat 32.811 kb on + strandat 32.844 kb on + strandat 32.845 kb on - strandat 32.880 kb on + strandat 32.880 kb on + strandat 32.880 kb on + strandat 32.881 kb on - strandat 32.881 kb on - strandat 32.881 kb on - strandat 32.943 kb on + strandat 32.944 kb on - strandat 33.271 kb on + strandat 33.272 kb on - strandat 33.275 kb on - strandat 33.275 kb on - strand, within Pf1N1B4_30at 33.639 kb on - strand, within Pf1N1B4_30at 33.639 kb on - strand, within Pf1N1B4_30at 33.655 kb on + strand, within Pf1N1B4_30at 33.916 kb on - strand, within Pf1N1B4_30at 34.061 kb on + strand, within Pf1N1B4_30at 34.083 kb on + strand, within Pf1N1B4_30at 34.084 kb on - strand, within Pf1N1B4_30at 34.084 kb on - strand, within Pf1N1B4_30at 34.110 kb on + strand, within Pf1N1B4_30at 34.111 kb on - strand, within Pf1N1B4_30at 34.187 kb on + strand, within Pf1N1B4_30at 34.188 kb on - strandat 34.188 kb on - strand, within Pf1N1B4_30at 34.188 kb on - strand, within Pf1N1B4_30at 34.190 kb on - strand, within Pf1N1B4_30at 34.368 kb on + strand, within Pf1N1B4_30at 34.368 kb on + strand, within Pf1N1B4_30at 34.369 kb on - strand, within Pf1N1B4_30at 34.384 kb on - strand, within Pf1N1B4_30at 34.451 kb on - strand, within Pf1N1B4_30at 34.451 kb on - strand, within Pf1N1B4_30at 34.454 kb on + strand, within Pf1N1B4_30at 34.455 kb on - strandat 34.455 kb on - strand, within Pf1N1B4_30at 34.455 kb on - strandat 34.518 kb on + strand, within Pf1N1B4_30at 34.556 kb on - strand, within Pf1N1B4_30at 34.733 kb on - strandat 34.733 kb on - strand, within Pf1N1B4_30at 34.913 kb on - strand, within Pf1N1B4_30at 34.913 kb on - strand, within Pf1N1B4_30at 35.033 kb on - strand, within Pf1N1B4_30at 35.164 kb on + strand, within Pf1N1B4_30at 35.165 kb on - strand, within Pf1N1B4_30at 35.320 kb on - strand, within Pf1N1B4_30at 35.463 kb on + strand, within Pf1N1B4_30at 35.600 kb on - strandat 35.610 kb on + strandat 35.611 kb on - strandat 35.611 kb on - strandat 35.662 kb on - strandat 35.699 kb on + strandat 35.812 kb on - strandat 36.245 kb on + strandat 36.245 kb on + strand, within Pf1N1B4_31at 36.370 kb on + strand, within Pf1N1B4_31at 36.370 kb on + strand, within Pf1N1B4_31at 36.479 kb on - strand, within Pf1N1B4_31at 36.479 kb on - strand, within Pf1N1B4_31at 36.479 kb on - strand, within Pf1N1B4_31at 36.479 kb on - strand, within Pf1N1B4_31at 36.560 kb on - strand, within Pf1N1B4_31at 36.750 kb on + strand, within Pf1N1B4_31at 36.751 kb on - strand, within Pf1N1B4_31at 36.751 kb on - strand, within Pf1N1B4_31

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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31,892 - -1.4
31,952 + -0.5
31,952 + +0.1
31,958 + +0.9
31,958 + -0.1
31,959 - +0.9
31,959 - +0.3
31,959 - +0.2
31,959 - +0.1
31,959 - +1.4
32,001 + -2.5
32,002 - -1.5
32,144 + Pf1N1B4_28 0.79 +0.7
32,170 + +1.4
32,171 - -0.9
32,171 - +1.5
32,223 - Pf1N1B4_29 0.18 -0.3
32,223 - Pf1N1B4_29 0.18 +0.6
32,232 - Pf1N1B4_29 0.22 -0.2
32,232 - Pf1N1B4_29 0.22 -2.3
32,232 - Pf1N1B4_29 0.22 -0.9
32,463 + +0.7
32,517 + -1.8
32,526 - +1.9
32,811 + +0.3
32,844 + +1.7
32,845 - -1.5
32,880 + -0.5
32,880 + -2.3
32,880 + +1.0
32,881 - -1.8
32,881 - +0.2
32,881 - -0.8
32,943 + +0.2
32,944 - -0.5
33,271 + -0.9
33,272 - +1.4
33,275 - +0.6
33,275 - Pf1N1B4_30 0.15 -0.5
33,639 - Pf1N1B4_30 0.27 -0.2
33,639 - Pf1N1B4_30 0.27 +0.1
33,655 + Pf1N1B4_30 0.27 -1.9
33,916 - Pf1N1B4_30 0.36 -1.1
34,061 + Pf1N1B4_30 0.40 -2.3
34,083 + Pf1N1B4_30 0.41 +1.3
34,084 - Pf1N1B4_30 0.41 +0.5
34,084 - Pf1N1B4_30 0.41 -0.8
34,110 + Pf1N1B4_30 0.42 -0.5
34,111 - Pf1N1B4_30 0.42 +0.2
34,187 + Pf1N1B4_30 0.45 -3.1
34,188 - -0.1
34,188 - Pf1N1B4_30 0.45 -1.8
34,188 - Pf1N1B4_30 0.45 -1.9
34,190 - Pf1N1B4_30 0.45 -1.1
34,368 + Pf1N1B4_30 0.50 +0.1
34,368 + Pf1N1B4_30 0.50 -0.3
34,369 - Pf1N1B4_30 0.50 -0.5
34,384 - Pf1N1B4_30 0.51 -1.4
34,451 - Pf1N1B4_30 0.53 -3.3
34,451 - Pf1N1B4_30 0.53 -0.7
34,454 + Pf1N1B4_30 0.53 +1.0
34,455 - -1.7
34,455 - Pf1N1B4_30 0.53 +2.1
34,455 - -1.1
34,518 + Pf1N1B4_30 0.55 +1.5
34,556 - Pf1N1B4_30 0.56 +0.2
34,733 - -1.7
34,733 - Pf1N1B4_30 0.62 +0.9
34,913 - Pf1N1B4_30 0.68 -0.9
34,913 - Pf1N1B4_30 0.68 -2.7
35,033 - Pf1N1B4_30 0.72 +0.1
35,164 + Pf1N1B4_30 0.76 -0.7
35,165 - Pf1N1B4_30 0.76 +1.0
35,320 - Pf1N1B4_30 0.81 +0.3
35,463 + Pf1N1B4_30 0.86 +0.1
35,600 - +0.7
35,610 + -1.6
35,611 - -0.1
35,611 - -1.5
35,662 - +1.8
35,699 + +0.4
35,812 - +1.9
36,245 + -1.2
36,245 + Pf1N1B4_31 0.31 -1.5
36,370 + Pf1N1B4_31 0.42 +0.3
36,370 + Pf1N1B4_31 0.42 -1.0
36,479 - Pf1N1B4_31 0.52 -1.8
36,479 - Pf1N1B4_31 0.52 -0.9
36,479 - Pf1N1B4_31 0.52 +0.5
36,479 - Pf1N1B4_31 0.52 +0.3
36,560 - Pf1N1B4_31 0.59 -2.2
36,750 + Pf1N1B4_31 0.76 -2.2
36,751 - Pf1N1B4_31 0.76 -0.6
36,751 - Pf1N1B4_31 0.76 -2.3

Or see this region's nucleotide sequence