Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2964

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2963 and Pf1N1B4_2964 are separated by 117 nucleotidesPf1N1B4_2964 and Pf1N1B4_2965 are separated by 168 nucleotides Pf1N1B4_2963 - Hypothetical metal-binding enzyme, YcbL homolog, at 3,324,818 to 3,325,462 _2963 Pf1N1B4_2964 - Outer membrane protein assembly factor YaeT precursor, at 3,325,580 to 3,326,302 _2964 Pf1N1B4_2965 - hypothetical protein, at 3,326,471 to 3,327,412 _2965 Position (kb) 3325 3326 3327Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3324.598 kb on + strandat 3324.728 kb on - strandat 3324.761 kb on + strandat 3324.761 kb on + strandat 3324.762 kb on - strandat 3324.783 kb on - strandat 3324.783 kb on - strandat 3325.136 kb on + strand, within Pf1N1B4_2963at 3325.137 kb on - strand, within Pf1N1B4_2963at 3325.137 kb on - strand, within Pf1N1B4_2963at 3325.137 kb on - strand, within Pf1N1B4_2963at 3325.137 kb on - strand, within Pf1N1B4_2963at 3325.138 kb on + strand, within Pf1N1B4_2963at 3325.138 kb on + strand, within Pf1N1B4_2963at 3325.139 kb on - strand, within Pf1N1B4_2963at 3325.225 kb on + strand, within Pf1N1B4_2963at 3325.226 kb on - strand, within Pf1N1B4_2963at 3325.226 kb on - strand, within Pf1N1B4_2963at 3325.226 kb on - strand, within Pf1N1B4_2963at 3325.282 kb on + strand, within Pf1N1B4_2963at 3325.318 kb on - strand, within Pf1N1B4_2963at 3325.318 kb on - strand, within Pf1N1B4_2963at 3325.367 kb on + strand, within Pf1N1B4_2963at 3325.367 kb on + strand, within Pf1N1B4_2963at 3325.367 kb on + strand, within Pf1N1B4_2963at 3325.367 kb on + strand, within Pf1N1B4_2963at 3325.367 kb on + strand, within Pf1N1B4_2963at 3325.367 kb on + strand, within Pf1N1B4_2963at 3325.367 kb on + strand, within Pf1N1B4_2963at 3325.367 kb on + strand, within Pf1N1B4_2963at 3325.367 kb on + strandat 3325.367 kb on + strand, within Pf1N1B4_2963at 3325.368 kb on - strand, within Pf1N1B4_2963at 3325.368 kb on - strand, within Pf1N1B4_2963at 3325.368 kb on - strand, within Pf1N1B4_2963at 3325.368 kb on - strand, within Pf1N1B4_2963at 3325.452 kb on + strandat 3325.453 kb on - strandat 3325.536 kb on + strandat 3325.536 kb on + strandat 3325.537 kb on - strandat 3325.537 kb on - strandat 3325.537 kb on - strandat 3325.568 kb on + strandat 3325.603 kb on + strandat 3325.604 kb on - strandat 3325.604 kb on - strandat 3325.655 kb on + strand, within Pf1N1B4_2964at 3325.655 kb on + strand, within Pf1N1B4_2964at 3325.655 kb on + strand, within Pf1N1B4_2964at 3325.655 kb on + strand, within Pf1N1B4_2964at 3325.655 kb on + strand, within Pf1N1B4_2964at 3325.712 kb on + strand, within Pf1N1B4_2964at 3325.712 kb on + strand, within Pf1N1B4_2964at 3325.712 kb on + strand, within Pf1N1B4_2964at 3325.713 kb on - strand, within Pf1N1B4_2964at 3325.713 kb on - strand, within Pf1N1B4_2964at 3325.836 kb on - strand, within Pf1N1B4_2964at 3325.836 kb on - strand, within Pf1N1B4_2964at 3325.836 kb on - strand, within Pf1N1B4_2964at 3325.979 kb on + strand, within Pf1N1B4_2964at 3325.979 kb on + strand, within Pf1N1B4_2964at 3325.980 kb on - strand, within Pf1N1B4_2964at 3326.120 kb on + strand, within Pf1N1B4_2964at 3326.120 kb on + strand, within Pf1N1B4_2964at 3326.120 kb on + strand, within Pf1N1B4_2964at 3326.120 kb on + strand, within Pf1N1B4_2964at 3326.120 kb on + strand, within Pf1N1B4_2964at 3326.121 kb on - strand, within Pf1N1B4_2964at 3326.121 kb on - strand, within Pf1N1B4_2964at 3326.121 kb on - strand, within Pf1N1B4_2964at 3326.121 kb on - strand, within Pf1N1B4_2964at 3326.121 kb on - strand, within Pf1N1B4_2964at 3326.268 kb on - strandat 3326.300 kb on + strandat 3326.300 kb on + strandat 3326.300 kb on + strandat 3326.300 kb on + strandat 3326.300 kb on + strandat 3326.300 kb on + strandat 3326.300 kb on + strandat 3326.300 kb on + strandat 3326.300 kb on + strandat 3326.301 kb on - strandat 3326.301 kb on - strandat 3326.301 kb on - strandat 3326.301 kb on - strandat 3326.324 kb on + strandat 3326.325 kb on - strandat 3326.325 kb on - strandat 3326.417 kb on - strandat 3326.425 kb on + strandat 3326.425 kb on + strandat 3326.425 kb on + strandat 3326.425 kb on + strandat 3326.426 kb on - strandat 3326.426 kb on - strandat 3326.426 kb on - strandat 3326.453 kb on - strandat 3326.453 kb on - strandat 3326.453 kb on - strandat 3326.453 kb on - strandat 3326.453 kb on - strandat 3326.453 kb on - strandat 3326.453 kb on - strandat 3326.453 kb on - strandat 3326.453 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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3,324,598 + -0.4
3,324,728 - -1.0
3,324,761 + +1.6
3,324,761 + +2.2
3,324,762 - +1.9
3,324,783 - +1.6
3,324,783 - -0.8
3,325,136 + Pf1N1B4_2963 0.49 -1.2
3,325,137 - Pf1N1B4_2963 0.49 -2.4
3,325,137 - Pf1N1B4_2963 0.49 +0.7
3,325,137 - Pf1N1B4_2963 0.49 -0.1
3,325,137 - Pf1N1B4_2963 0.49 -2.1
3,325,138 + Pf1N1B4_2963 0.50 -0.4
3,325,138 + Pf1N1B4_2963 0.50 +1.8
3,325,139 - Pf1N1B4_2963 0.50 +0.2
3,325,225 + Pf1N1B4_2963 0.63 +0.1
3,325,226 - Pf1N1B4_2963 0.63 +2.1
3,325,226 - Pf1N1B4_2963 0.63 +2.3
3,325,226 - Pf1N1B4_2963 0.63 +0.1
3,325,282 + Pf1N1B4_2963 0.72 +1.2
3,325,318 - Pf1N1B4_2963 0.78 +0.1
3,325,318 - Pf1N1B4_2963 0.78 -2.2
3,325,367 + Pf1N1B4_2963 0.85 -1.9
3,325,367 + Pf1N1B4_2963 0.85 +0.5
3,325,367 + Pf1N1B4_2963 0.85 -1.6
3,325,367 + Pf1N1B4_2963 0.85 -0.5
3,325,367 + Pf1N1B4_2963 0.85 +1.3
3,325,367 + Pf1N1B4_2963 0.85 -1.0
3,325,367 + Pf1N1B4_2963 0.85 -1.1
3,325,367 + Pf1N1B4_2963 0.85 +0.1
3,325,367 + +1.9
3,325,367 + Pf1N1B4_2963 0.85 -2.3
3,325,368 - Pf1N1B4_2963 0.85 -1.0
3,325,368 - Pf1N1B4_2963 0.85 -0.3
3,325,368 - Pf1N1B4_2963 0.85 -1.1
3,325,368 - Pf1N1B4_2963 0.85 -0.6
3,325,452 + -0.1
3,325,453 - -1.7
3,325,536 + -0.1
3,325,536 + +0.9
3,325,537 - -0.4
3,325,537 - -0.8
3,325,537 - +0.8
3,325,568 + -1.5
3,325,603 + -0.0
3,325,604 - +0.4
3,325,604 - -1.4
3,325,655 + Pf1N1B4_2964 0.10 +0.9
3,325,655 + Pf1N1B4_2964 0.10 +0.6
3,325,655 + Pf1N1B4_2964 0.10 -1.3
3,325,655 + Pf1N1B4_2964 0.10 +1.6
3,325,655 + Pf1N1B4_2964 0.10 -1.7
3,325,712 + Pf1N1B4_2964 0.18 +0.1
3,325,712 + Pf1N1B4_2964 0.18 +0.1
3,325,712 + Pf1N1B4_2964 0.18 +1.4
3,325,713 - Pf1N1B4_2964 0.18 -0.1
3,325,713 - Pf1N1B4_2964 0.18 -0.2
3,325,836 - Pf1N1B4_2964 0.35 +0.6
3,325,836 - Pf1N1B4_2964 0.35 +0.6
3,325,836 - Pf1N1B4_2964 0.35 -2.0
3,325,979 + Pf1N1B4_2964 0.55 +0.0
3,325,979 + Pf1N1B4_2964 0.55 -0.1
3,325,980 - Pf1N1B4_2964 0.55 +1.1
3,326,120 + Pf1N1B4_2964 0.75 +0.6
3,326,120 + Pf1N1B4_2964 0.75 +0.5
3,326,120 + Pf1N1B4_2964 0.75 +1.4
3,326,120 + Pf1N1B4_2964 0.75 +0.0
3,326,120 + Pf1N1B4_2964 0.75 -0.7
3,326,121 - Pf1N1B4_2964 0.75 +0.0
3,326,121 - Pf1N1B4_2964 0.75 -2.1
3,326,121 - Pf1N1B4_2964 0.75 +1.5
3,326,121 - Pf1N1B4_2964 0.75 +1.2
3,326,121 - Pf1N1B4_2964 0.75 +2.9
3,326,268 - +1.6
3,326,300 + -3.0
3,326,300 + +0.4
3,326,300 + +0.7
3,326,300 + +0.5
3,326,300 + +0.2
3,326,300 + -1.2
3,326,300 + +1.0
3,326,300 + -0.8
3,326,300 + +2.7
3,326,301 - -2.3
3,326,301 - +1.3
3,326,301 - +0.2
3,326,301 - -0.2
3,326,324 + -1.3
3,326,325 - +2.2
3,326,325 - +1.2
3,326,417 - +1.0
3,326,425 + -0.3
3,326,425 + +0.9
3,326,425 + +0.7
3,326,425 + +2.1
3,326,426 - -0.8
3,326,426 - +1.4
3,326,426 - +0.2
3,326,453 - +0.2
3,326,453 - -0.4
3,326,453 - -0.3
3,326,453 - +1.0
3,326,453 - -1.8
3,326,453 - -1.2
3,326,453 - +0.7
3,326,453 - +1.0
3,326,453 - +0.5

Or see this region's nucleotide sequence