Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2963 and Pf1N1B4_2964 are separated by 117 nucleotides Pf1N1B4_2964 and Pf1N1B4_2965 are separated by 168 nucleotides
Pf1N1B4_2963 - Hypothetical metal-binding enzyme, YcbL homolog, at 3,324,818 to 3,325,462
_2963
Pf1N1B4_2964 - Outer membrane protein assembly factor YaeT precursor, at 3,325,580 to 3,326,302
_2964
Pf1N1B4_2965 - hypothetical protein, at 3,326,471 to 3,327,412
_2965
Position (kb)
3325
3326
3327 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3324.598 kb on + strand at 3324.728 kb on - strand at 3324.761 kb on + strand at 3324.761 kb on + strand at 3324.762 kb on - strand at 3324.783 kb on - strand at 3324.783 kb on - strand at 3325.136 kb on + strand, within Pf1N1B4_2963 at 3325.137 kb on - strand, within Pf1N1B4_2963 at 3325.137 kb on - strand, within Pf1N1B4_2963 at 3325.137 kb on - strand, within Pf1N1B4_2963 at 3325.137 kb on - strand, within Pf1N1B4_2963 at 3325.138 kb on + strand, within Pf1N1B4_2963 at 3325.138 kb on + strand, within Pf1N1B4_2963 at 3325.139 kb on - strand, within Pf1N1B4_2963 at 3325.225 kb on + strand, within Pf1N1B4_2963 at 3325.226 kb on - strand, within Pf1N1B4_2963 at 3325.226 kb on - strand, within Pf1N1B4_2963 at 3325.226 kb on - strand, within Pf1N1B4_2963 at 3325.282 kb on + strand, within Pf1N1B4_2963 at 3325.318 kb on - strand, within Pf1N1B4_2963 at 3325.318 kb on - strand, within Pf1N1B4_2963 at 3325.367 kb on + strand, within Pf1N1B4_2963 at 3325.367 kb on + strand, within Pf1N1B4_2963 at 3325.367 kb on + strand, within Pf1N1B4_2963 at 3325.367 kb on + strand, within Pf1N1B4_2963 at 3325.367 kb on + strand, within Pf1N1B4_2963 at 3325.367 kb on + strand, within Pf1N1B4_2963 at 3325.367 kb on + strand, within Pf1N1B4_2963 at 3325.367 kb on + strand, within Pf1N1B4_2963 at 3325.367 kb on + strand at 3325.367 kb on + strand, within Pf1N1B4_2963 at 3325.368 kb on - strand, within Pf1N1B4_2963 at 3325.368 kb on - strand, within Pf1N1B4_2963 at 3325.368 kb on - strand, within Pf1N1B4_2963 at 3325.368 kb on - strand, within Pf1N1B4_2963 at 3325.452 kb on + strand at 3325.453 kb on - strand at 3325.536 kb on + strand at 3325.536 kb on + strand at 3325.537 kb on - strand at 3325.537 kb on - strand at 3325.537 kb on - strand at 3325.568 kb on + strand at 3325.603 kb on + strand at 3325.604 kb on - strand at 3325.604 kb on - strand at 3325.655 kb on + strand, within Pf1N1B4_2964 at 3325.655 kb on + strand, within Pf1N1B4_2964 at 3325.655 kb on + strand, within Pf1N1B4_2964 at 3325.655 kb on + strand, within Pf1N1B4_2964 at 3325.655 kb on + strand, within Pf1N1B4_2964 at 3325.712 kb on + strand, within Pf1N1B4_2964 at 3325.712 kb on + strand, within Pf1N1B4_2964 at 3325.712 kb on + strand, within Pf1N1B4_2964 at 3325.713 kb on - strand, within Pf1N1B4_2964 at 3325.713 kb on - strand, within Pf1N1B4_2964 at 3325.836 kb on - strand, within Pf1N1B4_2964 at 3325.836 kb on - strand, within Pf1N1B4_2964 at 3325.836 kb on - strand, within Pf1N1B4_2964 at 3325.979 kb on + strand, within Pf1N1B4_2964 at 3325.979 kb on + strand, within Pf1N1B4_2964 at 3325.980 kb on - strand, within Pf1N1B4_2964 at 3326.120 kb on + strand, within Pf1N1B4_2964 at 3326.120 kb on + strand, within Pf1N1B4_2964 at 3326.120 kb on + strand, within Pf1N1B4_2964 at 3326.120 kb on + strand, within Pf1N1B4_2964 at 3326.120 kb on + strand, within Pf1N1B4_2964 at 3326.121 kb on - strand, within Pf1N1B4_2964 at 3326.121 kb on - strand, within Pf1N1B4_2964 at 3326.121 kb on - strand, within Pf1N1B4_2964 at 3326.121 kb on - strand, within Pf1N1B4_2964 at 3326.121 kb on - strand, within Pf1N1B4_2964 at 3326.268 kb on - strand at 3326.300 kb on + strand at 3326.300 kb on + strand at 3326.300 kb on + strand at 3326.300 kb on + strand at 3326.300 kb on + strand at 3326.300 kb on + strand at 3326.300 kb on + strand at 3326.300 kb on + strand at 3326.300 kb on + strand at 3326.301 kb on - strand at 3326.301 kb on - strand at 3326.301 kb on - strand at 3326.301 kb on - strand at 3326.324 kb on + strand at 3326.325 kb on - strand at 3326.325 kb on - strand at 3326.417 kb on - strand at 3326.425 kb on + strand at 3326.425 kb on + strand at 3326.425 kb on + strand at 3326.425 kb on + strand at 3326.426 kb on - strand at 3326.426 kb on - strand at 3326.426 kb on - strand at 3326.453 kb on - strand at 3326.453 kb on - strand at 3326.453 kb on - strand at 3326.453 kb on - strand at 3326.453 kb on - strand at 3326.453 kb on - strand at 3326.453 kb on - strand at 3326.453 kb on - strand at 3326.453 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,324,598 + -0.4 3,324,728 - -1.0 3,324,761 + +1.6 3,324,761 + +2.2 3,324,762 - +1.9 3,324,783 - +1.6 3,324,783 - -0.8 3,325,136 + Pf1N1B4_2963 0.49 -1.2 3,325,137 - Pf1N1B4_2963 0.49 -2.4 3,325,137 - Pf1N1B4_2963 0.49 +0.7 3,325,137 - Pf1N1B4_2963 0.49 -0.1 3,325,137 - Pf1N1B4_2963 0.49 -2.1 3,325,138 + Pf1N1B4_2963 0.50 -0.4 3,325,138 + Pf1N1B4_2963 0.50 +1.8 3,325,139 - Pf1N1B4_2963 0.50 +0.2 3,325,225 + Pf1N1B4_2963 0.63 +0.1 3,325,226 - Pf1N1B4_2963 0.63 +2.1 3,325,226 - Pf1N1B4_2963 0.63 +2.3 3,325,226 - Pf1N1B4_2963 0.63 +0.1 3,325,282 + Pf1N1B4_2963 0.72 +1.2 3,325,318 - Pf1N1B4_2963 0.78 +0.1 3,325,318 - Pf1N1B4_2963 0.78 -2.2 3,325,367 + Pf1N1B4_2963 0.85 -1.9 3,325,367 + Pf1N1B4_2963 0.85 +0.5 3,325,367 + Pf1N1B4_2963 0.85 -1.6 3,325,367 + Pf1N1B4_2963 0.85 -0.5 3,325,367 + Pf1N1B4_2963 0.85 +1.3 3,325,367 + Pf1N1B4_2963 0.85 -1.0 3,325,367 + Pf1N1B4_2963 0.85 -1.1 3,325,367 + Pf1N1B4_2963 0.85 +0.1 3,325,367 + +1.9 3,325,367 + Pf1N1B4_2963 0.85 -2.3 3,325,368 - Pf1N1B4_2963 0.85 -1.0 3,325,368 - Pf1N1B4_2963 0.85 -0.3 3,325,368 - Pf1N1B4_2963 0.85 -1.1 3,325,368 - Pf1N1B4_2963 0.85 -0.6 3,325,452 + -0.1 3,325,453 - -1.7 3,325,536 + -0.1 3,325,536 + +0.9 3,325,537 - -0.4 3,325,537 - -0.8 3,325,537 - +0.8 3,325,568 + -1.5 3,325,603 + -0.0 3,325,604 - +0.4 3,325,604 - -1.4 3,325,655 + Pf1N1B4_2964 0.10 +0.9 3,325,655 + Pf1N1B4_2964 0.10 +0.6 3,325,655 + Pf1N1B4_2964 0.10 -1.3 3,325,655 + Pf1N1B4_2964 0.10 +1.6 3,325,655 + Pf1N1B4_2964 0.10 -1.7 3,325,712 + Pf1N1B4_2964 0.18 +0.1 3,325,712 + Pf1N1B4_2964 0.18 +0.1 3,325,712 + Pf1N1B4_2964 0.18 +1.4 3,325,713 - Pf1N1B4_2964 0.18 -0.1 3,325,713 - Pf1N1B4_2964 0.18 -0.2 3,325,836 - Pf1N1B4_2964 0.35 +0.6 3,325,836 - Pf1N1B4_2964 0.35 +0.6 3,325,836 - Pf1N1B4_2964 0.35 -2.0 3,325,979 + Pf1N1B4_2964 0.55 +0.0 3,325,979 + Pf1N1B4_2964 0.55 -0.1 3,325,980 - Pf1N1B4_2964 0.55 +1.1 3,326,120 + Pf1N1B4_2964 0.75 +0.6 3,326,120 + Pf1N1B4_2964 0.75 +0.5 3,326,120 + Pf1N1B4_2964 0.75 +1.4 3,326,120 + Pf1N1B4_2964 0.75 +0.0 3,326,120 + Pf1N1B4_2964 0.75 -0.7 3,326,121 - Pf1N1B4_2964 0.75 +0.0 3,326,121 - Pf1N1B4_2964 0.75 -2.1 3,326,121 - Pf1N1B4_2964 0.75 +1.5 3,326,121 - Pf1N1B4_2964 0.75 +1.2 3,326,121 - Pf1N1B4_2964 0.75 +2.9 3,326,268 - +1.6 3,326,300 + -3.0 3,326,300 + +0.4 3,326,300 + +0.7 3,326,300 + +0.5 3,326,300 + +0.2 3,326,300 + -1.2 3,326,300 + +1.0 3,326,300 + -0.8 3,326,300 + +2.7 3,326,301 - -2.3 3,326,301 - +1.3 3,326,301 - +0.2 3,326,301 - -0.2 3,326,324 + -1.3 3,326,325 - +2.2 3,326,325 - +1.2 3,326,417 - +1.0 3,326,425 + -0.3 3,326,425 + +0.9 3,326,425 + +0.7 3,326,425 + +2.1 3,326,426 - -0.8 3,326,426 - +1.4 3,326,426 - +0.2 3,326,453 - +0.2 3,326,453 - -0.4 3,326,453 - -0.3 3,326,453 - +1.0 3,326,453 - -1.8 3,326,453 - -1.2 3,326,453 - +0.7 3,326,453 - +1.0 3,326,453 - +0.5
Or see this region's nucleotide sequence