Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2636 and Pf1N1B4_2637 are separated by 32 nucleotides Pf1N1B4_2637 and Pf1N1B4_2638 overlap by 8 nucleotides Pf1N1B4_2638 and Pf1N1B4_2639 are separated by 157 nucleotides
Pf1N1B4_2636 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 2,977,295 to 2,977,411
_2636
Pf1N1B4_2637 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 2,977,444 to 2,978,163
_2637
Pf1N1B4_2638 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,978,156 to 2,979,085
_2638
Pf1N1B4_2639 - Outer membrane protein W precursor, at 2,979,243 to 2,979,941
_2639
Position (kb)
2978
2979
2980 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2977.160 kb on - strand at 2977.210 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.299 kb on + strand at 2977.299 kb on + strand at 2977.299 kb on + strand at 2977.459 kb on + strand at 2977.459 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.585 kb on + strand, within Pf1N1B4_2637 at 2977.618 kb on + strand, within Pf1N1B4_2637 at 2977.618 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2977.909 kb on + strand, within Pf1N1B4_2637 at 2978.135 kb on + strand at 2978.142 kb on + strand at 2978.142 kb on + strand at 2978.149 kb on + strand at 2978.149 kb on + strand at 2979.090 kb on - strand at 2979.244 kb on + strand at 2979.245 kb on - strand at 2979.245 kb on - strand at 2979.271 kb on + strand at 2979.271 kb on + strand at 2979.271 kb on + strand at 2979.271 kb on + strand at 2979.271 kb on + strand at 2979.271 kb on + strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.272 kb on - strand at 2979.364 kb on + strand, within Pf1N1B4_2639 at 2979.364 kb on + strand, within Pf1N1B4_2639 at 2979.365 kb on - strand, within Pf1N1B4_2639 at 2979.365 kb on - strand, within Pf1N1B4_2639 at 2979.365 kb on - strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.387 kb on + strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.388 kb on - strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.406 kb on + strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.407 kb on - strand, within Pf1N1B4_2639 at 2979.458 kb on + strand, within Pf1N1B4_2639 at 2979.458 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.514 kb on + strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.515 kb on - strand, within Pf1N1B4_2639 at 2979.521 kb on + strand, within Pf1N1B4_2639 at 2979.535 kb on + strand, within Pf1N1B4_2639 at 2979.535 kb on + strand, within Pf1N1B4_2639 at 2979.535 kb on + strand, within Pf1N1B4_2639 at 2979.535 kb on + strand, within Pf1N1B4_2639 at 2979.536 kb on - strand at 2979.536 kb on - strand, within Pf1N1B4_2639 at 2979.536 kb on - strand, within Pf1N1B4_2639 at 2979.536 kb on - strand, within Pf1N1B4_2639 at 2979.568 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.571 kb on + strand, within Pf1N1B4_2639 at 2979.572 kb on - strand, within Pf1N1B4_2639 at 2979.572 kb on - strand, within Pf1N1B4_2639 at 2979.572 kb on - strand, within Pf1N1B4_2639 at 2979.572 kb on - strand, within Pf1N1B4_2639 at 2979.715 kb on + strand, within Pf1N1B4_2639 at 2979.715 kb on + strand, within Pf1N1B4_2639 at 2979.716 kb on - strand, within Pf1N1B4_2639 at 2979.716 kb on - strand, within Pf1N1B4_2639 at 2979.984 kb on + strand at 2979.984 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 2,977,160 - +2.9 2,977,210 + -1.0 2,977,279 + +1.7 2,977,279 + -0.0 2,977,279 + +1.3 2,977,279 + -0.2 2,977,279 + +1.8 2,977,294 + +2.0 2,977,294 + +0.8 2,977,294 + +2.4 2,977,294 + +2.1 2,977,294 + +0.5 2,977,294 + +1.3 2,977,294 + +1.8 2,977,294 + +2.4 2,977,294 + +2.6 2,977,294 + +2.4 2,977,294 + +2.1 2,977,294 + +1.9 2,977,294 + +2.6 2,977,294 + +2.9 2,977,294 + +1.4 2,977,294 + +2.5 2,977,294 + +0.9 2,977,294 + -0.7 2,977,294 + +1.1 2,977,294 + +1.4 2,977,294 + +0.3 2,977,294 + +1.4 2,977,294 + +2.3 2,977,294 + +2.9 2,977,294 + +0.7 2,977,294 + +1.7 2,977,294 + +1.1 2,977,294 + +1.3 2,977,294 + +2.3 2,977,294 + +2.2 2,977,299 + +2.3 2,977,299 + +1.9 2,977,299 + +3.0 2,977,459 + +2.0 2,977,459 + +2.8 2,977,462 + +3.0 2,977,462 + +3.2 2,977,462 + +1.0 2,977,462 + +1.7 2,977,462 + +1.5 2,977,462 + +2.2 2,977,462 + +0.8 2,977,462 + +2.2 2,977,462 + +1.5 2,977,462 + +3.4 2,977,462 + +2.6 2,977,462 + +1.3 2,977,462 + +2.5 2,977,462 + +1.6 2,977,462 + +1.0 2,977,462 + +2.8 2,977,462 + +2.4 2,977,462 + +0.5 2,977,585 + Pf1N1B4_2637 0.20 +1.8 2,977,618 + Pf1N1B4_2637 0.24 +0.8 2,977,618 + Pf1N1B4_2637 0.24 +3.3 2,977,909 + Pf1N1B4_2637 0.65 +2.6 2,977,909 + Pf1N1B4_2637 0.65 +2.9 2,977,909 + Pf1N1B4_2637 0.65 +1.2 2,977,909 + Pf1N1B4_2637 0.65 +3.0 2,977,909 + Pf1N1B4_2637 0.65 +1.8 2,977,909 + Pf1N1B4_2637 0.65 +2.4 2,977,909 + Pf1N1B4_2637 0.65 +2.8 2,977,909 + Pf1N1B4_2637 0.65 +1.1 2,977,909 + Pf1N1B4_2637 0.65 +2.5 2,977,909 + Pf1N1B4_2637 0.65 +1.9 2,977,909 + Pf1N1B4_2637 0.65 +2.6 2,977,909 + +2.3 2,977,909 + Pf1N1B4_2637 0.65 -1.6 2,977,909 + Pf1N1B4_2637 0.65 +1.3 2,977,909 + Pf1N1B4_2637 0.65 +1.6 2,977,909 + Pf1N1B4_2637 0.65 +0.8 2,977,909 + Pf1N1B4_2637 0.65 +2.2 2,977,909 + Pf1N1B4_2637 0.65 +1.7 2,977,909 + Pf1N1B4_2637 0.65 +1.8 2,977,909 + Pf1N1B4_2637 0.65 +1.8 2,978,135 + +2.4 2,978,142 + +2.5 2,978,142 + +3.3 2,978,149 + +2.3 2,978,149 + +2.8 2,979,090 - -2.8 2,979,244 + -1.8 2,979,245 - +1.0 2,979,245 - -1.0 2,979,271 + -2.2 2,979,271 + +0.7 2,979,271 + +0.4 2,979,271 + +1.1 2,979,271 + -1.9 2,979,271 + +1.8 2,979,272 - -0.7 2,979,272 - -1.1 2,979,272 - +0.2 2,979,272 - -1.8 2,979,272 - +0.9 2,979,272 - +1.3 2,979,272 - -0.7 2,979,272 - +1.2 2,979,272 - -0.6 2,979,272 - -0.7 2,979,364 + Pf1N1B4_2639 0.17 +0.0 2,979,364 + Pf1N1B4_2639 0.17 -2.2 2,979,365 - Pf1N1B4_2639 0.17 -0.6 2,979,365 - Pf1N1B4_2639 0.17 +0.9 2,979,365 - Pf1N1B4_2639 0.17 -0.6 2,979,387 + Pf1N1B4_2639 0.21 -0.8 2,979,387 + Pf1N1B4_2639 0.21 -1.1 2,979,387 + Pf1N1B4_2639 0.21 +1.3 2,979,387 + Pf1N1B4_2639 0.21 -2.6 2,979,387 + Pf1N1B4_2639 0.21 -2.6 2,979,387 + Pf1N1B4_2639 0.21 +1.0 2,979,387 + Pf1N1B4_2639 0.21 -2.6 2,979,387 + Pf1N1B4_2639 0.21 -0.7 2,979,387 + Pf1N1B4_2639 0.21 +0.6 2,979,388 - Pf1N1B4_2639 0.21 +0.6 2,979,388 - Pf1N1B4_2639 0.21 -1.5 2,979,388 - Pf1N1B4_2639 0.21 -0.1 2,979,388 - Pf1N1B4_2639 0.21 -1.8 2,979,388 - Pf1N1B4_2639 0.21 -0.5 2,979,388 - Pf1N1B4_2639 0.21 +3.2 2,979,388 - Pf1N1B4_2639 0.21 -2.2 2,979,406 + Pf1N1B4_2639 0.23 +0.5 2,979,406 + Pf1N1B4_2639 0.23 -2.8 2,979,406 + Pf1N1B4_2639 0.23 +1.6 2,979,406 + Pf1N1B4_2639 0.23 -1.5 2,979,406 + Pf1N1B4_2639 0.23 -1.4 2,979,406 + Pf1N1B4_2639 0.23 +1.8 2,979,406 + Pf1N1B4_2639 0.23 -2.2 2,979,406 + Pf1N1B4_2639 0.23 +0.2 2,979,407 - Pf1N1B4_2639 0.23 -0.6 2,979,407 - Pf1N1B4_2639 0.23 -0.4 2,979,407 - Pf1N1B4_2639 0.23 -0.1 2,979,407 - Pf1N1B4_2639 0.23 +1.3 2,979,407 - Pf1N1B4_2639 0.23 -2.9 2,979,407 - Pf1N1B4_2639 0.23 -0.2 2,979,407 - Pf1N1B4_2639 0.23 +0.2 2,979,458 + Pf1N1B4_2639 0.31 +1.3 2,979,458 + Pf1N1B4_2639 0.31 -0.3 2,979,514 + Pf1N1B4_2639 0.39 -2.2 2,979,514 + Pf1N1B4_2639 0.39 -1.0 2,979,514 + Pf1N1B4_2639 0.39 -3.3 2,979,514 + Pf1N1B4_2639 0.39 +0.8 2,979,514 + Pf1N1B4_2639 0.39 +1.1 2,979,514 + Pf1N1B4_2639 0.39 -0.3 2,979,514 + Pf1N1B4_2639 0.39 +0.5 2,979,514 + Pf1N1B4_2639 0.39 -1.5 2,979,514 + Pf1N1B4_2639 0.39 -0.7 2,979,514 + Pf1N1B4_2639 0.39 +1.0 2,979,514 + Pf1N1B4_2639 0.39 -1.0 2,979,514 + Pf1N1B4_2639 0.39 +1.5 2,979,514 + Pf1N1B4_2639 0.39 -2.3 2,979,515 - Pf1N1B4_2639 0.39 +0.5 2,979,515 - Pf1N1B4_2639 0.39 -1.1 2,979,515 - Pf1N1B4_2639 0.39 -0.7 2,979,515 - Pf1N1B4_2639 0.39 +0.4 2,979,515 - Pf1N1B4_2639 0.39 +1.0 2,979,515 - Pf1N1B4_2639 0.39 -3.0 2,979,521 + Pf1N1B4_2639 0.40 +1.4 2,979,535 + Pf1N1B4_2639 0.42 -0.0 2,979,535 + Pf1N1B4_2639 0.42 +0.8 2,979,535 + Pf1N1B4_2639 0.42 +0.8 2,979,535 + Pf1N1B4_2639 0.42 -2.4 2,979,536 - -0.4 2,979,536 - Pf1N1B4_2639 0.42 -1.7 2,979,536 - Pf1N1B4_2639 0.42 -0.8 2,979,536 - Pf1N1B4_2639 0.42 -0.0 2,979,568 + Pf1N1B4_2639 0.46 +0.1 2,979,571 + Pf1N1B4_2639 0.47 +0.7 2,979,571 + Pf1N1B4_2639 0.47 -0.5 2,979,571 + Pf1N1B4_2639 0.47 -3.1 2,979,571 + Pf1N1B4_2639 0.47 -0.9 2,979,571 + Pf1N1B4_2639 0.47 -1.8 2,979,571 + Pf1N1B4_2639 0.47 -2.1 2,979,572 - Pf1N1B4_2639 0.47 +1.0 2,979,572 - Pf1N1B4_2639 0.47 +1.0 2,979,572 - Pf1N1B4_2639 0.47 +0.9 2,979,572 - Pf1N1B4_2639 0.47 -0.3 2,979,715 + Pf1N1B4_2639 0.68 -2.7 2,979,715 + Pf1N1B4_2639 0.68 +0.4 2,979,716 - Pf1N1B4_2639 0.68 +1.0 2,979,716 - Pf1N1B4_2639 0.68 +0.7 2,979,984 + -0.9 2,979,984 + -1.5
Or see this region's nucleotide sequence