Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2557 and Pf1N1B4_2558 are separated by 33 nucleotides Pf1N1B4_2558 and Pf1N1B4_2559 are separated by 135 nucleotides Pf1N1B4_2559 and Pf1N1B4_2560 are separated by 13 nucleotides Pf1N1B4_2560 and Pf1N1B4_2561 are separated by 38 nucleotides Pf1N1B4_2561 and Pf1N1B4_2562 are separated by 74 nucleotides
Pf1N1B4_2557 - 2-nitropropane dioxygenase (EC 1.13.11.32), at 2,899,322 to 2,900,290
_2557
Pf1N1B4_2558 - Protoporphyrinogen IX oxidase, novel form, HemJ (EC 1.3.-.-), at 2,900,324 to 2,900,755
_2558
Pf1N1B4_2559 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 2,900,891 to 2,901,925
_2559
Pf1N1B4_2560 - hypothetical protein, at 2,901,939 to 2,902,055
_2560
Pf1N1B4_2561 - probable iron binding protein from the HesB_IscA_SufA family, at 2,902,094 to 2,902,444
_2561
Pf1N1B4_2562 - Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-), at 2,902,519 to 2,903,610
_2562
Position (kb)
2900
2901
2902 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2900.000 kb on + strand, within Pf1N1B4_2557 at 2900.076 kb on + strand, within Pf1N1B4_2557 at 2900.076 kb on + strand at 2900.180 kb on + strand, within Pf1N1B4_2557 at 2900.224 kb on + strand at 2900.225 kb on - strand at 2900.243 kb on + strand at 2900.244 kb on - strand at 2900.244 kb on - strand at 2900.325 kb on + strand at 2900.325 kb on + strand at 2900.326 kb on - strand at 2900.760 kb on - strand at 2900.760 kb on - strand at 2900.760 kb on - strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.792 kb on + strand at 2900.792 kb on + strand at 2900.849 kb on + strand at 2900.849 kb on + strand at 2900.890 kb on + strand at 2900.890 kb on + strand at 2902.001 kb on + strand, within Pf1N1B4_2560 at 2902.002 kb on - strand at 2902.002 kb on - strand, within Pf1N1B4_2560 at 2902.003 kb on + strand, within Pf1N1B4_2560 at 2902.003 kb on + strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.008 kb on + strand, within Pf1N1B4_2560 at 2902.009 kb on - strand, within Pf1N1B4_2560 at 2902.014 kb on + strand, within Pf1N1B4_2560 at 2902.063 kb on + strand at 2902.459 kb on + strand at 2902.459 kb on + strand at 2902.459 kb on + strand at 2902.459 kb on + strand at 2902.460 kb on - strand at 2902.460 kb on - strand at 2902.460 kb on - strand at 2902.460 kb on - strand at 2902.460 kb on - strand at 2902.460 kb on - strand at 2902.462 kb on + strand at 2902.463 kb on - strand at 2902.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 2,899,941 + Pf1N1B4_2557 0.64 -1.4 2,899,941 + Pf1N1B4_2557 0.64 -0.3 2,899,941 + Pf1N1B4_2557 0.64 -2.5 2,900,000 + Pf1N1B4_2557 0.70 -0.4 2,900,076 + Pf1N1B4_2557 0.78 +0.1 2,900,076 + +0.0 2,900,180 + Pf1N1B4_2557 0.89 -3.1 2,900,224 + +2.1 2,900,225 - -2.1 2,900,243 + -0.8 2,900,244 - +2.3 2,900,244 - -0.6 2,900,325 + +0.7 2,900,325 + -0.6 2,900,326 - -1.6 2,900,760 - -0.3 2,900,760 - +0.1 2,900,760 - -0.3 2,900,789 + +0.7 2,900,789 + +2.3 2,900,789 + -1.3 2,900,789 + +2.3 2,900,789 + +1.5 2,900,789 + +1.5 2,900,789 + +2.6 2,900,789 + +0.8 2,900,789 + +1.1 2,900,789 + +2.4 2,900,789 + +2.1 2,900,789 + +2.5 2,900,789 + +0.2 2,900,789 + +1.0 2,900,789 + +1.6 2,900,789 + -0.1 2,900,790 - +0.0 2,900,790 - -0.3 2,900,790 - -1.1 2,900,790 - -0.3 2,900,790 - -0.4 2,900,792 + -0.3 2,900,792 + -1.3 2,900,849 + +1.2 2,900,849 + +1.0 2,900,890 + +1.4 2,900,890 + -0.3 2,902,001 + Pf1N1B4_2560 0.53 -1.1 2,902,002 - +0.3 2,902,002 - Pf1N1B4_2560 0.54 -1.6 2,902,003 + Pf1N1B4_2560 0.55 -1.0 2,902,003 + Pf1N1B4_2560 0.55 -1.1 2,902,004 - Pf1N1B4_2560 0.56 -1.7 2,902,004 - Pf1N1B4_2560 0.56 +0.1 2,902,004 - Pf1N1B4_2560 0.56 +0.7 2,902,004 - Pf1N1B4_2560 0.56 -2.2 2,902,004 - Pf1N1B4_2560 0.56 -0.5 2,902,004 - Pf1N1B4_2560 0.56 +0.0 2,902,004 - Pf1N1B4_2560 0.56 -0.9 2,902,004 - Pf1N1B4_2560 0.56 -1.5 2,902,004 - Pf1N1B4_2560 0.56 -1.1 2,902,004 - Pf1N1B4_2560 0.56 -2.1 2,902,004 - Pf1N1B4_2560 0.56 -0.7 2,902,004 - Pf1N1B4_2560 0.56 -0.5 2,902,008 + Pf1N1B4_2560 0.59 +0.4 2,902,009 - Pf1N1B4_2560 0.60 -0.5 2,902,014 + Pf1N1B4_2560 0.64 -1.0 2,902,063 + +1.1 2,902,459 + +0.4 2,902,459 + +0.5 2,902,459 + +0.1 2,902,459 + +0.3 2,902,460 - +1.8 2,902,460 - -0.5 2,902,460 - -1.8 2,902,460 - -1.6 2,902,460 - +1.5 2,902,460 - +0.4 2,902,462 + -1.4 2,902,463 - -1.1 2,902,463 - +2.3
Or see this region's nucleotide sequence