Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1786 and Pf1N1B4_1787 are separated by 228 nucleotides Pf1N1B4_1787 and Pf1N1B4_1788 are separated by 177 nucleotides Pf1N1B4_1788 and Pf1N1B4_1789 are separated by 69 nucleotides
Pf1N1B4_1786 - ATP-dependent DNA helicase, RecQ family, at 2,038,340 to 2,040,274
_1786
Pf1N1B4_1787 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,040,503 to 2,041,729
_1787
Pf1N1B4_1788 - putative 4-hydroxybenzoyl-CoA thioesterase, at 2,041,907 to 2,042,338
_1788
Pf1N1B4_1789 - FIG01270322: hypothetical protein, at 2,042,408 to 2,042,896
_1789
Position (kb)
2040
2041
2042 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2039.546 kb on + strand, within Pf1N1B4_1786 at 2039.552 kb on + strand, within Pf1N1B4_1786 at 2039.552 kb on + strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.577 kb on - strand at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.699 kb on + strand, within Pf1N1B4_1786 at 2039.699 kb on + strand, within Pf1N1B4_1786 at 2039.700 kb on - strand, within Pf1N1B4_1786 at 2039.708 kb on + strand, within Pf1N1B4_1786 at 2039.709 kb on - strand, within Pf1N1B4_1786 at 2039.747 kb on + strand, within Pf1N1B4_1786 at 2039.748 kb on - strand, within Pf1N1B4_1786 at 2039.748 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.858 kb on + strand, within Pf1N1B4_1786 at 2039.859 kb on - strand, within Pf1N1B4_1786 at 2039.954 kb on + strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.175 kb on + strand at 2040.176 kb on - strand at 2040.225 kb on - strand at 2040.401 kb on - strand at 2040.401 kb on - strand at 2040.462 kb on - strand at 2040.462 kb on - strand at 2040.488 kb on - strand at 2040.665 kb on + strand, within Pf1N1B4_1787 at 2040.665 kb on + strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.869 kb on - strand, within Pf1N1B4_1787 at 2040.869 kb on - strand, within Pf1N1B4_1787 at 2041.021 kb on - strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.104 kb on - strand, within Pf1N1B4_1787 at 2041.155 kb on + strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.206 kb on - strand, within Pf1N1B4_1787 at 2041.206 kb on - strand, within Pf1N1B4_1787 at 2041.337 kb on + strand, within Pf1N1B4_1787 at 2041.338 kb on - strand, within Pf1N1B4_1787 at 2041.340 kb on + strand, within Pf1N1B4_1787 at 2041.340 kb on + strand, within Pf1N1B4_1787 at 2041.340 kb on + strand, within Pf1N1B4_1787 at 2041.341 kb on - strand, within Pf1N1B4_1787 at 2041.547 kb on + strand, within Pf1N1B4_1787 at 2041.547 kb on + strand, within Pf1N1B4_1787 at 2041.548 kb on - strand, within Pf1N1B4_1787 at 2041.622 kb on - strand at 2041.734 kb on - strand at 2041.734 kb on - strand at 2041.734 kb on - strand at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2041.986 kb on - strand at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.013 kb on - strand, within Pf1N1B4_1788 at 2042.013 kb on - strand, within Pf1N1B4_1788 at 2042.013 kb on - strand, within Pf1N1B4_1788 at 2042.042 kb on + strand, within Pf1N1B4_1788 at 2042.042 kb on + strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.117 kb on + strand, within Pf1N1B4_1788 at 2042.117 kb on + strand, within Pf1N1B4_1788 at 2042.117 kb on + strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.196 kb on - strand, within Pf1N1B4_1788 at 2042.383 kb on - strand at 2042.428 kb on + strand at 2042.428 kb on + strand at 2042.428 kb on + strand at 2042.428 kb on + strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.503 kb on - strand, within Pf1N1B4_1789 at 2042.503 kb on - strand, within Pf1N1B4_1789 at 2042.503 kb on - strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.641 kb on + strand, within Pf1N1B4_1789
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 2,039,546 + Pf1N1B4_1786 0.62 -1.5 2,039,552 + Pf1N1B4_1786 0.63 -1.3 2,039,552 + Pf1N1B4_1786 0.63 +1.3 2,039,553 - Pf1N1B4_1786 0.63 -0.8 2,039,553 - Pf1N1B4_1786 0.63 +0.4 2,039,553 - Pf1N1B4_1786 0.63 -2.6 2,039,576 + Pf1N1B4_1786 0.64 -2.4 2,039,576 + Pf1N1B4_1786 0.64 -0.1 2,039,576 + Pf1N1B4_1786 0.64 -0.9 2,039,576 + Pf1N1B4_1786 0.64 +1.7 2,039,576 + Pf1N1B4_1786 0.64 -0.5 2,039,577 - +0.9 2,039,577 - Pf1N1B4_1786 0.64 -1.2 2,039,577 - Pf1N1B4_1786 0.64 +1.6 2,039,577 - Pf1N1B4_1786 0.64 -1.1 2,039,699 + Pf1N1B4_1786 0.70 +1.0 2,039,699 + Pf1N1B4_1786 0.70 +1.2 2,039,700 - Pf1N1B4_1786 0.70 +0.5 2,039,708 + Pf1N1B4_1786 0.71 -1.6 2,039,709 - Pf1N1B4_1786 0.71 -2.2 2,039,747 + Pf1N1B4_1786 0.73 -1.0 2,039,748 - Pf1N1B4_1786 0.73 +0.5 2,039,748 - Pf1N1B4_1786 0.73 +1.7 2,039,802 - Pf1N1B4_1786 0.76 +0.1 2,039,802 - Pf1N1B4_1786 0.76 -1.0 2,039,802 - Pf1N1B4_1786 0.76 +0.5 2,039,858 + Pf1N1B4_1786 0.78 +1.9 2,039,859 - Pf1N1B4_1786 0.79 +1.4 2,039,954 + Pf1N1B4_1786 0.83 +1.3 2,039,955 - Pf1N1B4_1786 0.83 -0.1 2,039,955 - Pf1N1B4_1786 0.83 +1.2 2,039,955 - Pf1N1B4_1786 0.83 +0.3 2,040,075 + Pf1N1B4_1786 0.90 -1.8 2,040,075 + +0.9 2,040,075 + Pf1N1B4_1786 0.90 -0.7 2,040,075 + Pf1N1B4_1786 0.90 -2.2 2,040,075 + Pf1N1B4_1786 0.90 +0.2 2,040,075 + Pf1N1B4_1786 0.90 -0.6 2,040,076 - Pf1N1B4_1786 0.90 +1.3 2,040,076 - Pf1N1B4_1786 0.90 -0.6 2,040,076 - Pf1N1B4_1786 0.90 -0.5 2,040,175 + +2.4 2,040,176 - -0.0 2,040,225 - +1.8 2,040,401 - +1.8 2,040,401 - -0.0 2,040,462 - -1.7 2,040,462 - -0.7 2,040,488 - +1.8 2,040,665 + Pf1N1B4_1787 0.13 +0.8 2,040,665 + Pf1N1B4_1787 0.13 +2.0 2,040,666 - Pf1N1B4_1787 0.13 +1.0 2,040,666 - Pf1N1B4_1787 0.13 -3.5 2,040,666 - Pf1N1B4_1787 0.13 +2.6 2,040,666 - Pf1N1B4_1787 0.13 -0.8 2,040,868 + Pf1N1B4_1787 0.30 -0.6 2,040,868 + Pf1N1B4_1787 0.30 +0.4 2,040,868 + Pf1N1B4_1787 0.30 +0.8 2,040,868 + Pf1N1B4_1787 0.30 -0.6 2,040,868 + Pf1N1B4_1787 0.30 -1.6 2,040,869 - Pf1N1B4_1787 0.30 -1.3 2,040,869 - Pf1N1B4_1787 0.30 +1.1 2,041,021 - Pf1N1B4_1787 0.42 +1.0 2,041,103 + Pf1N1B4_1787 0.49 +0.2 2,041,103 + Pf1N1B4_1787 0.49 +0.7 2,041,103 + Pf1N1B4_1787 0.49 -3.8 2,041,104 - Pf1N1B4_1787 0.49 -0.2 2,041,155 + Pf1N1B4_1787 0.53 -2.0 2,041,156 - Pf1N1B4_1787 0.53 -0.3 2,041,156 - Pf1N1B4_1787 0.53 +1.2 2,041,156 - Pf1N1B4_1787 0.53 +1.0 2,041,156 - Pf1N1B4_1787 0.53 -1.8 2,041,205 + Pf1N1B4_1787 0.57 -0.5 2,041,205 + Pf1N1B4_1787 0.57 -2.1 2,041,205 + Pf1N1B4_1787 0.57 +2.7 2,041,205 + Pf1N1B4_1787 0.57 +0.8 2,041,205 + Pf1N1B4_1787 0.57 -1.1 2,041,205 + Pf1N1B4_1787 0.57 +0.8 2,041,206 - Pf1N1B4_1787 0.57 -0.1 2,041,206 - Pf1N1B4_1787 0.57 -0.5 2,041,337 + Pf1N1B4_1787 0.68 +0.7 2,041,338 - Pf1N1B4_1787 0.68 +1.9 2,041,340 + Pf1N1B4_1787 0.68 +0.2 2,041,340 + Pf1N1B4_1787 0.68 +0.2 2,041,340 + Pf1N1B4_1787 0.68 +1.4 2,041,341 - Pf1N1B4_1787 0.68 +0.2 2,041,547 + Pf1N1B4_1787 0.85 -0.7 2,041,547 + Pf1N1B4_1787 0.85 +1.9 2,041,548 - Pf1N1B4_1787 0.85 -0.7 2,041,622 - +0.1 2,041,734 - -0.7 2,041,734 - +0.1 2,041,734 - -0.5 2,041,985 + Pf1N1B4_1788 0.18 +1.5 2,041,985 + Pf1N1B4_1788 0.18 +0.7 2,041,985 + Pf1N1B4_1788 0.18 -0.5 2,041,985 + Pf1N1B4_1788 0.18 -1.3 2,041,985 + Pf1N1B4_1788 0.18 -1.2 2,041,985 + Pf1N1B4_1788 0.18 +0.8 2,041,985 + Pf1N1B4_1788 0.18 -0.9 2,041,985 + Pf1N1B4_1788 0.18 -0.4 2,041,986 - Pf1N1B4_1788 0.18 +0.3 2,041,986 - Pf1N1B4_1788 0.18 +0.7 2,041,986 - +1.7 2,041,986 - Pf1N1B4_1788 0.18 -0.2 2,041,986 - Pf1N1B4_1788 0.18 -1.1 2,042,009 + Pf1N1B4_1788 0.24 -1.3 2,042,009 + Pf1N1B4_1788 0.24 -0.3 2,042,009 + Pf1N1B4_1788 0.24 -0.5 2,042,009 + Pf1N1B4_1788 0.24 +0.6 2,042,010 - Pf1N1B4_1788 0.24 +2.0 2,042,010 - Pf1N1B4_1788 0.24 +0.4 2,042,010 - Pf1N1B4_1788 0.24 +0.7 2,042,010 - Pf1N1B4_1788 0.24 +0.3 2,042,010 - Pf1N1B4_1788 0.24 -0.3 2,042,010 - Pf1N1B4_1788 0.24 -2.5 2,042,012 + Pf1N1B4_1788 0.24 -1.7 2,042,012 + Pf1N1B4_1788 0.24 -0.4 2,042,012 + Pf1N1B4_1788 0.24 +0.9 2,042,012 + Pf1N1B4_1788 0.24 -2.4 2,042,013 - Pf1N1B4_1788 0.25 -1.8 2,042,013 - Pf1N1B4_1788 0.25 +0.4 2,042,013 - Pf1N1B4_1788 0.25 +2.1 2,042,042 + Pf1N1B4_1788 0.31 -2.1 2,042,042 + Pf1N1B4_1788 0.31 +0.2 2,042,043 - Pf1N1B4_1788 0.31 +0.1 2,042,043 - Pf1N1B4_1788 0.31 -1.5 2,042,043 - Pf1N1B4_1788 0.31 -1.7 2,042,043 - Pf1N1B4_1788 0.31 -0.7 2,042,117 + Pf1N1B4_1788 0.49 -1.8 2,042,117 + Pf1N1B4_1788 0.49 -0.3 2,042,117 + Pf1N1B4_1788 0.49 +0.3 2,042,118 - Pf1N1B4_1788 0.49 +1.5 2,042,118 - Pf1N1B4_1788 0.49 +0.2 2,042,118 - Pf1N1B4_1788 0.49 -0.5 2,042,118 - Pf1N1B4_1788 0.49 -1.7 2,042,196 - Pf1N1B4_1788 0.67 -1.9 2,042,383 - +0.1 2,042,428 + +0.7 2,042,428 + -0.3 2,042,428 + -1.9 2,042,428 + -0.8 2,042,429 - +0.3 2,042,429 - -0.8 2,042,429 - -1.8 2,042,429 - -0.2 2,042,429 - -0.6 2,042,429 - -0.8 2,042,503 - Pf1N1B4_1789 0.19 -2.1 2,042,503 - Pf1N1B4_1789 0.19 +1.0 2,042,503 - Pf1N1B4_1789 0.19 -2.0 2,042,553 + Pf1N1B4_1789 0.30 +1.6 2,042,553 + Pf1N1B4_1789 0.30 +0.8 2,042,553 + Pf1N1B4_1789 0.30 -0.3 2,042,553 + Pf1N1B4_1789 0.30 -0.8 2,042,553 + Pf1N1B4_1789 0.30 -2.4 2,042,553 + Pf1N1B4_1789 0.30 -0.0 2,042,553 + Pf1N1B4_1789 0.30 +0.2 2,042,554 - Pf1N1B4_1789 0.30 -0.5 2,042,554 - Pf1N1B4_1789 0.30 -2.1 2,042,554 - Pf1N1B4_1789 0.30 -0.0 2,042,554 - Pf1N1B4_1789 0.30 -3.1 2,042,554 - Pf1N1B4_1789 0.30 -0.5 2,042,554 - Pf1N1B4_1789 0.30 -1.0 2,042,554 - Pf1N1B4_1789 0.30 -2.1 2,042,554 - Pf1N1B4_1789 0.30 +0.7 2,042,554 - Pf1N1B4_1789 0.30 -1.7 2,042,554 - Pf1N1B4_1789 0.30 +0.1 2,042,554 - Pf1N1B4_1789 0.30 +0.3 2,042,554 - Pf1N1B4_1789 0.30 -0.7 2,042,554 - Pf1N1B4_1789 0.30 -2.5 2,042,554 - Pf1N1B4_1789 0.30 +0.6 2,042,641 + Pf1N1B4_1789 0.48 -1.3
Or see this region's nucleotide sequence