Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1663 and Pf1N1B4_1664 are separated by 886 nucleotides Pf1N1B4_1664 and Pf1N1B4_1665 are separated by 160 nucleotides
Pf1N1B4_1663 - 4-aminobutyrate aminotransferase (EC 2.6.1.19), at 1,904,166 to 1,907,078
_1663
Pf1N1B4_1664 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 1,907,965 to 1,908,510
_1664
Pf1N1B4_1665 - Signal transduction histidine kinase regulating C4-dicarboxylate transport system, at 1,908,671 to 1,910,425
_1665
Position (kb)
1907
1908
1909 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1907.079 kb on - strand at 1907.079 kb on - strand at 1907.098 kb on + strand at 1907.109 kb on + strand at 1907.109 kb on + strand at 1907.109 kb on + strand at 1907.109 kb on + strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.111 kb on + strand at 1907.112 kb on - strand at 1907.112 kb on - strand at 1907.145 kb on + strand at 1907.146 kb on - strand at 1907.146 kb on - strand at 1907.147 kb on + strand at 1907.147 kb on + strand at 1907.148 kb on - strand at 1907.210 kb on + strand at 1907.210 kb on + strand at 1907.253 kb on + strand at 1907.253 kb on + strand at 1907.253 kb on + strand at 1907.253 kb on + strand at 1907.253 kb on + strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.428 kb on + strand at 1907.466 kb on - strand at 1907.466 kb on - strand at 1907.537 kb on + strand at 1907.537 kb on + strand at 1907.537 kb on + strand at 1907.537 kb on + strand at 1907.537 kb on + strand at 1907.537 kb on + strand at 1907.538 kb on - strand at 1907.538 kb on - strand at 1907.538 kb on - strand at 1907.538 kb on - strand at 1907.538 kb on - strand at 1907.547 kb on + strand at 1907.547 kb on + strand at 1907.577 kb on - strand at 1907.586 kb on + strand at 1907.626 kb on + strand at 1907.626 kb on + strand at 1907.627 kb on - strand at 1907.627 kb on - strand at 1907.641 kb on + strand at 1907.642 kb on - strand at 1907.642 kb on - strand at 1907.677 kb on + strand at 1907.678 kb on - strand at 1907.785 kb on + strand at 1907.785 kb on + strand at 1907.785 kb on + strand at 1907.786 kb on - strand at 1907.786 kb on - strand at 1907.786 kb on - strand at 1907.803 kb on + strand at 1907.804 kb on - strand at 1907.848 kb on + strand at 1907.848 kb on + strand at 1907.926 kb on - strand at 1907.948 kb on - strand at 1907.948 kb on - strand at 1907.972 kb on + strand at 1907.972 kb on + strand at 1907.972 kb on + strand at 1907.972 kb on + strand at 1907.973 kb on - strand at 1907.973 kb on - strand at 1907.973 kb on - strand at 1907.973 kb on - strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.043 kb on + strand, within Pf1N1B4_1664 at 1908.043 kb on + strand, within Pf1N1B4_1664 at 1908.043 kb on + strand, within Pf1N1B4_1664 at 1908.043 kb on + strand, within Pf1N1B4_1664 at 1908.044 kb on - strand, within Pf1N1B4_1664 at 1908.044 kb on - strand, within Pf1N1B4_1664 at 1908.134 kb on + strand, within Pf1N1B4_1664 at 1908.151 kb on + strand, within Pf1N1B4_1664 at 1908.355 kb on + strand, within Pf1N1B4_1664 at 1908.356 kb on - strand, within Pf1N1B4_1664 at 1908.370 kb on + strand, within Pf1N1B4_1664 at 1908.370 kb on + strand, within Pf1N1B4_1664 at 1908.370 kb on + strand, within Pf1N1B4_1664 at 1908.370 kb on + strand, within Pf1N1B4_1664 at 1908.371 kb on - strand, within Pf1N1B4_1664 at 1908.373 kb on + strand, within Pf1N1B4_1664 at 1908.373 kb on + strand, within Pf1N1B4_1664 at 1908.373 kb on + strand, within Pf1N1B4_1664 at 1908.373 kb on + strand, within Pf1N1B4_1664 at 1908.373 kb on + strand, within Pf1N1B4_1664 at 1908.374 kb on - strand, within Pf1N1B4_1664 at 1908.374 kb on - strand, within Pf1N1B4_1664 at 1908.455 kb on + strand, within Pf1N1B4_1664 at 1908.455 kb on + strand, within Pf1N1B4_1664 at 1908.456 kb on - strand at 1908.456 kb on - strand at 1908.584 kb on + strand at 1908.584 kb on + strand at 1908.584 kb on + strand at 1908.585 kb on - strand at 1908.585 kb on - strand at 1908.593 kb on + strand at 1908.593 kb on + strand at 1908.594 kb on - strand at 1908.595 kb on + strand at 1908.596 kb on - strand at 1908.722 kb on + strand at 1908.800 kb on + strand at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.939 kb on - strand, within Pf1N1B4_1665 at 1908.939 kb on - strand, within Pf1N1B4_1665 at 1908.939 kb on - strand, within Pf1N1B4_1665 at 1908.939 kb on - strand, within Pf1N1B4_1665 at 1909.028 kb on + strand, within Pf1N1B4_1665 at 1909.028 kb on + strand, within Pf1N1B4_1665 at 1909.028 kb on + strand, within Pf1N1B4_1665 at 1909.029 kb on - strand, within Pf1N1B4_1665 at 1909.100 kb on + strand, within Pf1N1B4_1665 at 1909.101 kb on - strand, within Pf1N1B4_1665 at 1909.491 kb on + strand, within Pf1N1B4_1665 at 1909.491 kb on + strand, within Pf1N1B4_1665 at 1909.492 kb on - strand, within Pf1N1B4_1665
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 1,907,079 - -1.2 1,907,079 - -2.8 1,907,098 + -0.4 1,907,109 + +0.9 1,907,109 + +0.4 1,907,109 + -1.0 1,907,109 + +2.3 1,907,110 - -0.5 1,907,110 - -2.3 1,907,110 - -0.4 1,907,110 - +1.9 1,907,110 - -0.1 1,907,110 - -1.7 1,907,110 - -0.8 1,907,110 - +0.0 1,907,110 - -0.5 1,907,111 + +0.5 1,907,112 - -1.3 1,907,112 - -0.6 1,907,145 + +0.9 1,907,146 - -0.4 1,907,146 - -2.9 1,907,147 + -0.9 1,907,147 + -1.3 1,907,148 - -1.2 1,907,210 + +0.7 1,907,210 + +0.9 1,907,253 + -0.8 1,907,253 + +0.3 1,907,253 + +0.5 1,907,253 + -0.1 1,907,253 + -1.2 1,907,254 - +1.8 1,907,254 - +1.5 1,907,254 - -0.9 1,907,254 - +1.9 1,907,254 - +0.3 1,907,254 - +1.0 1,907,254 - -2.1 1,907,254 - +0.3 1,907,428 + -0.9 1,907,466 - -2.0 1,907,466 - -1.6 1,907,537 + -1.5 1,907,537 + +0.9 1,907,537 + +2.4 1,907,537 + +0.8 1,907,537 + -1.7 1,907,537 + -0.5 1,907,538 - +1.4 1,907,538 - +1.8 1,907,538 - +2.1 1,907,538 - -0.6 1,907,538 - +2.5 1,907,547 + -1.5 1,907,547 + -0.9 1,907,577 - +0.3 1,907,586 + -1.6 1,907,626 + -0.4 1,907,626 + -0.5 1,907,627 - +0.4 1,907,627 - +1.3 1,907,641 + +1.4 1,907,642 - -0.6 1,907,642 - +2.8 1,907,677 + +0.4 1,907,678 - +1.5 1,907,785 + -1.7 1,907,785 + -3.5 1,907,785 + +2.7 1,907,786 - +0.7 1,907,786 - +0.2 1,907,786 - -0.5 1,907,803 + -0.7 1,907,804 - +1.4 1,907,848 + +0.5 1,907,848 + -0.9 1,907,926 - -0.6 1,907,948 - -2.7 1,907,948 - -1.4 1,907,972 + -1.3 1,907,972 + -2.0 1,907,972 + +0.3 1,907,972 + +1.1 1,907,973 - +0.2 1,907,973 - +1.3 1,907,973 - +0.3 1,907,973 - -0.9 1,908,017 + +0.8 1,908,017 + +1.0 1,908,017 + -1.7 1,908,017 + +0.6 1,908,017 + -1.2 1,908,017 + -2.9 1,908,017 + -1.7 1,908,018 - +1.3 1,908,018 - -1.0 1,908,018 - -0.5 1,908,018 - +0.6 1,908,018 - +1.8 1,908,018 - +1.0 1,908,018 - +0.5 1,908,018 - +2.8 1,908,043 + Pf1N1B4_1664 0.14 +0.4 1,908,043 + Pf1N1B4_1664 0.14 +1.7 1,908,043 + Pf1N1B4_1664 0.14 -1.6 1,908,043 + Pf1N1B4_1664 0.14 +1.5 1,908,044 - Pf1N1B4_1664 0.14 -1.7 1,908,044 - Pf1N1B4_1664 0.14 +0.8 1,908,134 + Pf1N1B4_1664 0.31 -1.9 1,908,151 + Pf1N1B4_1664 0.34 -2.1 1,908,355 + Pf1N1B4_1664 0.71 -1.5 1,908,356 - Pf1N1B4_1664 0.72 -1.3 1,908,370 + Pf1N1B4_1664 0.74 -1.7 1,908,370 + Pf1N1B4_1664 0.74 +0.2 1,908,370 + Pf1N1B4_1664 0.74 -0.7 1,908,370 + Pf1N1B4_1664 0.74 -1.4 1,908,371 - Pf1N1B4_1664 0.74 -0.5 1,908,373 + Pf1N1B4_1664 0.75 -0.9 1,908,373 + Pf1N1B4_1664 0.75 +0.8 1,908,373 + Pf1N1B4_1664 0.75 -1.3 1,908,373 + Pf1N1B4_1664 0.75 +0.9 1,908,373 + Pf1N1B4_1664 0.75 +0.1 1,908,374 - Pf1N1B4_1664 0.75 +2.8 1,908,374 - Pf1N1B4_1664 0.75 +0.2 1,908,455 + Pf1N1B4_1664 0.90 -1.2 1,908,455 + Pf1N1B4_1664 0.90 +1.4 1,908,456 - -1.0 1,908,456 - -0.9 1,908,584 + -2.2 1,908,584 + -0.1 1,908,584 + +0.8 1,908,585 - -0.4 1,908,585 - +0.1 1,908,593 + -1.5 1,908,593 + +1.4 1,908,594 - +1.7 1,908,595 + +0.8 1,908,596 - -2.6 1,908,722 + -2.7 1,908,800 + -1.5 1,908,938 + Pf1N1B4_1665 0.15 -0.7 1,908,938 + Pf1N1B4_1665 0.15 +1.1 1,908,938 + Pf1N1B4_1665 0.15 -3.3 1,908,938 + Pf1N1B4_1665 0.15 -1.9 1,908,938 + Pf1N1B4_1665 0.15 -2.4 1,908,938 + Pf1N1B4_1665 0.15 -0.5 1,908,938 + Pf1N1B4_1665 0.15 -1.7 1,908,938 + Pf1N1B4_1665 0.15 -0.0 1,908,939 - Pf1N1B4_1665 0.15 -0.7 1,908,939 - Pf1N1B4_1665 0.15 -0.2 1,908,939 - Pf1N1B4_1665 0.15 -0.7 1,908,939 - Pf1N1B4_1665 0.15 +0.7 1,909,028 + Pf1N1B4_1665 0.20 -1.8 1,909,028 + Pf1N1B4_1665 0.20 -1.7 1,909,028 + Pf1N1B4_1665 0.20 +0.2 1,909,029 - Pf1N1B4_1665 0.20 +0.2 1,909,100 + Pf1N1B4_1665 0.24 +0.9 1,909,101 - Pf1N1B4_1665 0.25 -2.0 1,909,491 + Pf1N1B4_1665 0.47 -0.1 1,909,491 + Pf1N1B4_1665 0.47 -2.8 1,909,492 - Pf1N1B4_1665 0.47 +1.5
Or see this region's nucleotide sequence