Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1446 and Pf1N1B4_1447 are separated by 184 nucleotides Pf1N1B4_1447 and Pf1N1B4_1448 are separated by 464 nucleotides
Pf1N1B4_1446 - Predicted hydroxymethylpyrimidine transporter CytX, at 1,657,113 to 1,658,366
_1446
Pf1N1B4_1447 - Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17), at 1,658,551 to 1,660,440
_1447
Pf1N1B4_1448 - Type I secretion outer membrane protein, TolC precursor, at 1,660,905 to 1,662,344
_1448
Position (kb)
1658
1659
1660
1661 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1657.630 kb on + strand at 1657.630 kb on + strand, within Pf1N1B4_1446 at 1657.868 kb on + strand, within Pf1N1B4_1446 at 1657.869 kb on - strand, within Pf1N1B4_1446 at 1657.869 kb on - strand, within Pf1N1B4_1446 at 1657.869 kb on - strand, within Pf1N1B4_1446 at 1657.869 kb on - strand, within Pf1N1B4_1446 at 1657.869 kb on - strand, within Pf1N1B4_1446 at 1657.893 kb on - strand, within Pf1N1B4_1446 at 1658.383 kb on + strand at 1658.383 kb on + strand at 1658.383 kb on + strand at 1658.384 kb on - strand at 1658.384 kb on - strand at 1658.384 kb on - strand at 1658.384 kb on - strand at 1658.384 kb on - strand at 1658.412 kb on + strand at 1658.412 kb on + strand at 1658.413 kb on - strand at 1658.413 kb on - strand at 1658.413 kb on - strand at 1658.413 kb on - strand at 1658.413 kb on - strand at 1658.565 kb on - strand at 1658.565 kb on - strand at 1658.765 kb on + strand, within Pf1N1B4_1447 at 1658.766 kb on - strand, within Pf1N1B4_1447 at 1658.766 kb on - strand, within Pf1N1B4_1447 at 1658.909 kb on + strand, within Pf1N1B4_1447 at 1658.909 kb on + strand, within Pf1N1B4_1447 at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.910 kb on - strand at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.910 kb on - strand, within Pf1N1B4_1447 at 1658.975 kb on + strand, within Pf1N1B4_1447 at 1658.975 kb on + strand, within Pf1N1B4_1447 at 1658.976 kb on - strand, within Pf1N1B4_1447 at 1659.080 kb on + strand, within Pf1N1B4_1447 at 1659.081 kb on - strand, within Pf1N1B4_1447 at 1659.081 kb on - strand, within Pf1N1B4_1447 at 1659.175 kb on + strand, within Pf1N1B4_1447 at 1659.175 kb on + strand, within Pf1N1B4_1447 at 1659.175 kb on + strand, within Pf1N1B4_1447 at 1659.175 kb on + strand, within Pf1N1B4_1447 at 1659.175 kb on + strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.176 kb on - strand, within Pf1N1B4_1447 at 1659.302 kb on + strand, within Pf1N1B4_1447 at 1659.302 kb on + strand, within Pf1N1B4_1447 at 1659.342 kb on - strand, within Pf1N1B4_1447 at 1659.357 kb on + strand, within Pf1N1B4_1447 at 1659.357 kb on + strand, within Pf1N1B4_1447 at 1659.358 kb on - strand, within Pf1N1B4_1447 at 1659.473 kb on + strand, within Pf1N1B4_1447 at 1659.473 kb on + strand, within Pf1N1B4_1447 at 1659.474 kb on - strand, within Pf1N1B4_1447 at 1659.569 kb on + strand, within Pf1N1B4_1447 at 1659.569 kb on + strand, within Pf1N1B4_1447 at 1659.569 kb on + strand, within Pf1N1B4_1447 at 1659.570 kb on - strand, within Pf1N1B4_1447 at 1659.604 kb on - strand, within Pf1N1B4_1447 at 1659.971 kb on + strand, within Pf1N1B4_1447 at 1659.971 kb on + strand, within Pf1N1B4_1447 at 1659.971 kb on + strand, within Pf1N1B4_1447 at 1659.972 kb on - strand, within Pf1N1B4_1447 at 1659.993 kb on + strand, within Pf1N1B4_1447 at 1659.994 kb on - strand, within Pf1N1B4_1447 at 1660.008 kb on - strand, within Pf1N1B4_1447 at 1660.223 kb on + strand, within Pf1N1B4_1447 at 1660.223 kb on + strand, within Pf1N1B4_1447 at 1660.223 kb on + strand, within Pf1N1B4_1447 at 1660.224 kb on - strand, within Pf1N1B4_1447 at 1660.241 kb on + strand at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.346 kb on + strand at 1660.346 kb on + strand at 1660.347 kb on - strand at 1660.495 kb on + strand at 1660.669 kb on + strand at 1660.687 kb on + strand at 1660.687 kb on + strand at 1660.687 kb on + strand at 1660.687 kb on + strand at 1660.688 kb on - strand at 1660.688 kb on - strand at 1660.721 kb on - strand at 1660.721 kb on - strand at 1660.809 kb on + strand at 1660.809 kb on + strand at 1660.809 kb on + strand at 1660.809 kb on + strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1661.196 kb on + strand, within Pf1N1B4_1448 at 1661.197 kb on - strand, within Pf1N1B4_1448
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 1,657,630 + +0.8 1,657,630 + Pf1N1B4_1446 0.41 -0.1 1,657,868 + Pf1N1B4_1446 0.60 -0.6 1,657,869 - Pf1N1B4_1446 0.60 +0.2 1,657,869 - Pf1N1B4_1446 0.60 +0.3 1,657,869 - Pf1N1B4_1446 0.60 -1.6 1,657,869 - Pf1N1B4_1446 0.60 +0.5 1,657,869 - Pf1N1B4_1446 0.60 +0.8 1,657,893 - Pf1N1B4_1446 0.62 -0.0 1,658,383 + -1.3 1,658,383 + -1.2 1,658,383 + -0.1 1,658,384 - -0.8 1,658,384 - -2.8 1,658,384 - +0.2 1,658,384 - -1.6 1,658,384 - +0.7 1,658,412 + +0.2 1,658,412 + +0.4 1,658,413 - -2.5 1,658,413 - -0.7 1,658,413 - +0.1 1,658,413 - +0.8 1,658,413 - -2.2 1,658,565 - -2.0 1,658,565 - -0.8 1,658,765 + Pf1N1B4_1447 0.11 -2.0 1,658,766 - Pf1N1B4_1447 0.11 -2.3 1,658,766 - Pf1N1B4_1447 0.11 +1.2 1,658,909 + Pf1N1B4_1447 0.19 -1.5 1,658,909 + Pf1N1B4_1447 0.19 -2.5 1,658,910 - Pf1N1B4_1447 0.19 -2.1 1,658,910 - Pf1N1B4_1447 0.19 -0.4 1,658,910 - Pf1N1B4_1447 0.19 -2.6 1,658,910 - Pf1N1B4_1447 0.19 -1.6 1,658,910 - +0.1 1,658,910 - Pf1N1B4_1447 0.19 +0.3 1,658,910 - Pf1N1B4_1447 0.19 +0.2 1,658,975 + Pf1N1B4_1447 0.22 -2.7 1,658,975 + Pf1N1B4_1447 0.22 -2.6 1,658,976 - Pf1N1B4_1447 0.22 -0.5 1,659,080 + Pf1N1B4_1447 0.28 -0.5 1,659,081 - Pf1N1B4_1447 0.28 -2.6 1,659,081 - Pf1N1B4_1447 0.28 -0.1 1,659,175 + Pf1N1B4_1447 0.33 -1.0 1,659,175 + Pf1N1B4_1447 0.33 -1.3 1,659,175 + Pf1N1B4_1447 0.33 -1.9 1,659,175 + Pf1N1B4_1447 0.33 -2.0 1,659,175 + Pf1N1B4_1447 0.33 -1.8 1,659,176 - Pf1N1B4_1447 0.33 -0.5 1,659,176 - Pf1N1B4_1447 0.33 -2.0 1,659,176 - Pf1N1B4_1447 0.33 -0.1 1,659,176 - Pf1N1B4_1447 0.33 -2.4 1,659,176 - Pf1N1B4_1447 0.33 -0.2 1,659,176 - Pf1N1B4_1447 0.33 -1.9 1,659,176 - Pf1N1B4_1447 0.33 -1.8 1,659,302 + Pf1N1B4_1447 0.40 -1.6 1,659,302 + Pf1N1B4_1447 0.40 -0.9 1,659,342 - Pf1N1B4_1447 0.42 -2.1 1,659,357 + Pf1N1B4_1447 0.43 +0.7 1,659,357 + Pf1N1B4_1447 0.43 -3.6 1,659,358 - Pf1N1B4_1447 0.43 -1.8 1,659,473 + Pf1N1B4_1447 0.49 +0.1 1,659,473 + Pf1N1B4_1447 0.49 -1.0 1,659,474 - Pf1N1B4_1447 0.49 -0.8 1,659,569 + Pf1N1B4_1447 0.54 -0.5 1,659,569 + Pf1N1B4_1447 0.54 -2.2 1,659,569 + Pf1N1B4_1447 0.54 -2.0 1,659,570 - Pf1N1B4_1447 0.54 -0.9 1,659,604 - Pf1N1B4_1447 0.56 -2.5 1,659,971 + Pf1N1B4_1447 0.75 -0.5 1,659,971 + Pf1N1B4_1447 0.75 -1.0 1,659,971 + Pf1N1B4_1447 0.75 -0.8 1,659,972 - Pf1N1B4_1447 0.75 -3.2 1,659,993 + Pf1N1B4_1447 0.76 -2.2 1,659,994 - Pf1N1B4_1447 0.76 -0.4 1,660,008 - Pf1N1B4_1447 0.77 -1.8 1,660,223 + Pf1N1B4_1447 0.88 -2.2 1,660,223 + Pf1N1B4_1447 0.88 -0.5 1,660,223 + Pf1N1B4_1447 0.88 -1.1 1,660,224 - Pf1N1B4_1447 0.89 -1.2 1,660,241 + +0.8 1,660,241 + Pf1N1B4_1447 0.89 -2.6 1,660,241 + Pf1N1B4_1447 0.89 -1.1 1,660,241 + Pf1N1B4_1447 0.89 +1.1 1,660,241 + Pf1N1B4_1447 0.89 -1.7 1,660,242 - Pf1N1B4_1447 0.89 -1.8 1,660,242 - Pf1N1B4_1447 0.89 -1.8 1,660,242 - Pf1N1B4_1447 0.89 -3.4 1,660,242 - -0.6 1,660,242 - Pf1N1B4_1447 0.89 -0.6 1,660,242 - Pf1N1B4_1447 0.89 -2.9 1,660,346 + +0.3 1,660,346 + -1.6 1,660,347 - -1.6 1,660,495 + -2.0 1,660,669 + -1.8 1,660,687 + +0.7 1,660,687 + -1.8 1,660,687 + -1.4 1,660,687 + -0.2 1,660,688 - -1.6 1,660,688 - -1.6 1,660,721 - -1.4 1,660,721 - -0.4 1,660,809 + -0.4 1,660,809 + -0.5 1,660,809 + -2.4 1,660,809 + -2.0 1,660,810 - -2.2 1,660,810 - -0.3 1,660,810 - -1.7 1,660,810 - -0.5 1,660,810 - +0.0 1,660,810 - -0.1 1,661,196 + Pf1N1B4_1448 0.20 +0.1 1,661,197 - Pf1N1B4_1448 0.20 +1.5
Or see this region's nucleotide sequence