Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1447

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1446 and Pf1N1B4_1447 are separated by 184 nucleotidesPf1N1B4_1447 and Pf1N1B4_1448 are separated by 464 nucleotides Pf1N1B4_1446 - Predicted hydroxymethylpyrimidine transporter CytX, at 1,657,113 to 1,658,366 _1446 Pf1N1B4_1447 - Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17), at 1,658,551 to 1,660,440 _1447 Pf1N1B4_1448 - Type I secretion outer membrane protein, TolC precursor, at 1,660,905 to 1,662,344 _1448 Position (kb) 1658 1659 1660 1661Strain fitness (log2 ratio) -3 -2 -1 0 1at 1657.630 kb on + strandat 1657.630 kb on + strand, within Pf1N1B4_1446at 1657.868 kb on + strand, within Pf1N1B4_1446at 1657.869 kb on - strand, within Pf1N1B4_1446at 1657.869 kb on - strand, within Pf1N1B4_1446at 1657.869 kb on - strand, within Pf1N1B4_1446at 1657.869 kb on - strand, within Pf1N1B4_1446at 1657.869 kb on - strand, within Pf1N1B4_1446at 1657.893 kb on - strand, within Pf1N1B4_1446at 1658.383 kb on + strandat 1658.383 kb on + strandat 1658.383 kb on + strandat 1658.384 kb on - strandat 1658.384 kb on - strandat 1658.384 kb on - strandat 1658.384 kb on - strandat 1658.384 kb on - strandat 1658.412 kb on + strandat 1658.412 kb on + strandat 1658.413 kb on - strandat 1658.413 kb on - strandat 1658.413 kb on - strandat 1658.413 kb on - strandat 1658.413 kb on - strandat 1658.565 kb on - strandat 1658.565 kb on - strandat 1658.765 kb on + strand, within Pf1N1B4_1447at 1658.766 kb on - strand, within Pf1N1B4_1447at 1658.766 kb on - strand, within Pf1N1B4_1447at 1658.909 kb on + strand, within Pf1N1B4_1447at 1658.909 kb on + strand, within Pf1N1B4_1447at 1658.910 kb on - strand, within Pf1N1B4_1447at 1658.910 kb on - strand, within Pf1N1B4_1447at 1658.910 kb on - strand, within Pf1N1B4_1447at 1658.910 kb on - strand, within Pf1N1B4_1447at 1658.910 kb on - strandat 1658.910 kb on - strand, within Pf1N1B4_1447at 1658.910 kb on - strand, within Pf1N1B4_1447at 1658.975 kb on + strand, within Pf1N1B4_1447at 1658.975 kb on + strand, within Pf1N1B4_1447at 1658.976 kb on - strand, within Pf1N1B4_1447at 1659.080 kb on + strand, within Pf1N1B4_1447at 1659.081 kb on - strand, within Pf1N1B4_1447at 1659.081 kb on - strand, within Pf1N1B4_1447at 1659.175 kb on + strand, within Pf1N1B4_1447at 1659.175 kb on + strand, within Pf1N1B4_1447at 1659.175 kb on + strand, within Pf1N1B4_1447at 1659.175 kb on + strand, within Pf1N1B4_1447at 1659.175 kb on + strand, within Pf1N1B4_1447at 1659.176 kb on - strand, within Pf1N1B4_1447at 1659.176 kb on - strand, within Pf1N1B4_1447at 1659.176 kb on - strand, within Pf1N1B4_1447at 1659.176 kb on - strand, within Pf1N1B4_1447at 1659.176 kb on - strand, within Pf1N1B4_1447at 1659.176 kb on - strand, within Pf1N1B4_1447at 1659.176 kb on - strand, within Pf1N1B4_1447at 1659.302 kb on + strand, within Pf1N1B4_1447at 1659.302 kb on + strand, within Pf1N1B4_1447at 1659.342 kb on - strand, within Pf1N1B4_1447at 1659.357 kb on + strand, within Pf1N1B4_1447at 1659.357 kb on + strand, within Pf1N1B4_1447at 1659.358 kb on - strand, within Pf1N1B4_1447at 1659.473 kb on + strand, within Pf1N1B4_1447at 1659.473 kb on + strand, within Pf1N1B4_1447at 1659.474 kb on - strand, within Pf1N1B4_1447at 1659.569 kb on + strand, within Pf1N1B4_1447at 1659.569 kb on + strand, within Pf1N1B4_1447at 1659.569 kb on + strand, within Pf1N1B4_1447at 1659.570 kb on - strand, within Pf1N1B4_1447at 1659.604 kb on - strand, within Pf1N1B4_1447at 1659.971 kb on + strand, within Pf1N1B4_1447at 1659.971 kb on + strand, within Pf1N1B4_1447at 1659.971 kb on + strand, within Pf1N1B4_1447at 1659.972 kb on - strand, within Pf1N1B4_1447at 1659.993 kb on + strand, within Pf1N1B4_1447at 1659.994 kb on - strand, within Pf1N1B4_1447at 1660.008 kb on - strand, within Pf1N1B4_1447at 1660.223 kb on + strand, within Pf1N1B4_1447at 1660.223 kb on + strand, within Pf1N1B4_1447at 1660.223 kb on + strand, within Pf1N1B4_1447at 1660.224 kb on - strand, within Pf1N1B4_1447at 1660.241 kb on + strandat 1660.241 kb on + strand, within Pf1N1B4_1447at 1660.241 kb on + strand, within Pf1N1B4_1447at 1660.241 kb on + strand, within Pf1N1B4_1447at 1660.241 kb on + strand, within Pf1N1B4_1447at 1660.242 kb on - strand, within Pf1N1B4_1447at 1660.242 kb on - strand, within Pf1N1B4_1447at 1660.242 kb on - strand, within Pf1N1B4_1447at 1660.242 kb on - strandat 1660.242 kb on - strand, within Pf1N1B4_1447at 1660.242 kb on - strand, within Pf1N1B4_1447at 1660.346 kb on + strandat 1660.346 kb on + strandat 1660.347 kb on - strandat 1660.495 kb on + strandat 1660.669 kb on + strandat 1660.687 kb on + strandat 1660.687 kb on + strandat 1660.687 kb on + strandat 1660.687 kb on + strandat 1660.688 kb on - strandat 1660.688 kb on - strandat 1660.721 kb on - strandat 1660.721 kb on - strandat 1660.809 kb on + strandat 1660.809 kb on + strandat 1660.809 kb on + strandat 1660.809 kb on + strandat 1660.810 kb on - strandat 1660.810 kb on - strandat 1660.810 kb on - strandat 1660.810 kb on - strandat 1660.810 kb on - strandat 1660.810 kb on - strandat 1661.196 kb on + strand, within Pf1N1B4_1448at 1661.197 kb on - strand, within Pf1N1B4_1448

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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1,657,630 + +0.8
1,657,630 + Pf1N1B4_1446 0.41 -0.1
1,657,868 + Pf1N1B4_1446 0.60 -0.6
1,657,869 - Pf1N1B4_1446 0.60 +0.2
1,657,869 - Pf1N1B4_1446 0.60 +0.3
1,657,869 - Pf1N1B4_1446 0.60 -1.6
1,657,869 - Pf1N1B4_1446 0.60 +0.5
1,657,869 - Pf1N1B4_1446 0.60 +0.8
1,657,893 - Pf1N1B4_1446 0.62 -0.0
1,658,383 + -1.3
1,658,383 + -1.2
1,658,383 + -0.1
1,658,384 - -0.8
1,658,384 - -2.8
1,658,384 - +0.2
1,658,384 - -1.6
1,658,384 - +0.7
1,658,412 + +0.2
1,658,412 + +0.4
1,658,413 - -2.5
1,658,413 - -0.7
1,658,413 - +0.1
1,658,413 - +0.8
1,658,413 - -2.2
1,658,565 - -2.0
1,658,565 - -0.8
1,658,765 + Pf1N1B4_1447 0.11 -2.0
1,658,766 - Pf1N1B4_1447 0.11 -2.3
1,658,766 - Pf1N1B4_1447 0.11 +1.2
1,658,909 + Pf1N1B4_1447 0.19 -1.5
1,658,909 + Pf1N1B4_1447 0.19 -2.5
1,658,910 - Pf1N1B4_1447 0.19 -2.1
1,658,910 - Pf1N1B4_1447 0.19 -0.4
1,658,910 - Pf1N1B4_1447 0.19 -2.6
1,658,910 - Pf1N1B4_1447 0.19 -1.6
1,658,910 - +0.1
1,658,910 - Pf1N1B4_1447 0.19 +0.3
1,658,910 - Pf1N1B4_1447 0.19 +0.2
1,658,975 + Pf1N1B4_1447 0.22 -2.7
1,658,975 + Pf1N1B4_1447 0.22 -2.6
1,658,976 - Pf1N1B4_1447 0.22 -0.5
1,659,080 + Pf1N1B4_1447 0.28 -0.5
1,659,081 - Pf1N1B4_1447 0.28 -2.6
1,659,081 - Pf1N1B4_1447 0.28 -0.1
1,659,175 + Pf1N1B4_1447 0.33 -1.0
1,659,175 + Pf1N1B4_1447 0.33 -1.3
1,659,175 + Pf1N1B4_1447 0.33 -1.9
1,659,175 + Pf1N1B4_1447 0.33 -2.0
1,659,175 + Pf1N1B4_1447 0.33 -1.8
1,659,176 - Pf1N1B4_1447 0.33 -0.5
1,659,176 - Pf1N1B4_1447 0.33 -2.0
1,659,176 - Pf1N1B4_1447 0.33 -0.1
1,659,176 - Pf1N1B4_1447 0.33 -2.4
1,659,176 - Pf1N1B4_1447 0.33 -0.2
1,659,176 - Pf1N1B4_1447 0.33 -1.9
1,659,176 - Pf1N1B4_1447 0.33 -1.8
1,659,302 + Pf1N1B4_1447 0.40 -1.6
1,659,302 + Pf1N1B4_1447 0.40 -0.9
1,659,342 - Pf1N1B4_1447 0.42 -2.1
1,659,357 + Pf1N1B4_1447 0.43 +0.7
1,659,357 + Pf1N1B4_1447 0.43 -3.6
1,659,358 - Pf1N1B4_1447 0.43 -1.8
1,659,473 + Pf1N1B4_1447 0.49 +0.1
1,659,473 + Pf1N1B4_1447 0.49 -1.0
1,659,474 - Pf1N1B4_1447 0.49 -0.8
1,659,569 + Pf1N1B4_1447 0.54 -0.5
1,659,569 + Pf1N1B4_1447 0.54 -2.2
1,659,569 + Pf1N1B4_1447 0.54 -2.0
1,659,570 - Pf1N1B4_1447 0.54 -0.9
1,659,604 - Pf1N1B4_1447 0.56 -2.5
1,659,971 + Pf1N1B4_1447 0.75 -0.5
1,659,971 + Pf1N1B4_1447 0.75 -1.0
1,659,971 + Pf1N1B4_1447 0.75 -0.8
1,659,972 - Pf1N1B4_1447 0.75 -3.2
1,659,993 + Pf1N1B4_1447 0.76 -2.2
1,659,994 - Pf1N1B4_1447 0.76 -0.4
1,660,008 - Pf1N1B4_1447 0.77 -1.8
1,660,223 + Pf1N1B4_1447 0.88 -2.2
1,660,223 + Pf1N1B4_1447 0.88 -0.5
1,660,223 + Pf1N1B4_1447 0.88 -1.1
1,660,224 - Pf1N1B4_1447 0.89 -1.2
1,660,241 + +0.8
1,660,241 + Pf1N1B4_1447 0.89 -2.6
1,660,241 + Pf1N1B4_1447 0.89 -1.1
1,660,241 + Pf1N1B4_1447 0.89 +1.1
1,660,241 + Pf1N1B4_1447 0.89 -1.7
1,660,242 - Pf1N1B4_1447 0.89 -1.8
1,660,242 - Pf1N1B4_1447 0.89 -1.8
1,660,242 - Pf1N1B4_1447 0.89 -3.4
1,660,242 - -0.6
1,660,242 - Pf1N1B4_1447 0.89 -0.6
1,660,242 - Pf1N1B4_1447 0.89 -2.9
1,660,346 + +0.3
1,660,346 + -1.6
1,660,347 - -1.6
1,660,495 + -2.0
1,660,669 + -1.8
1,660,687 + +0.7
1,660,687 + -1.8
1,660,687 + -1.4
1,660,687 + -0.2
1,660,688 - -1.6
1,660,688 - -1.6
1,660,721 - -1.4
1,660,721 - -0.4
1,660,809 + -0.4
1,660,809 + -0.5
1,660,809 + -2.4
1,660,809 + -2.0
1,660,810 - -2.2
1,660,810 - -0.3
1,660,810 - -1.7
1,660,810 - -0.5
1,660,810 - +0.0
1,660,810 - -0.1
1,661,196 + Pf1N1B4_1448 0.20 +0.1
1,661,197 - Pf1N1B4_1448 0.20 +1.5

Or see this region's nucleotide sequence