Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02730 and MMP_RS02735 are separated by 27 nucleotides MMP_RS02735 and MMP_RS02740 are separated by 80 nucleotides MMP_RS02740 and MMP_RS02745 are separated by 96 nucleotides MMP_RS02745 and MMP_RS02750 are separated by 15 nucleotides
MMP_RS02730: MMP_RS02730 - ModD protein, at 521,710 to 522,561
_RS02730
MMP_RS02735: MMP_RS02735 - MBL fold metallo-hydrolase, at 522,589 to 523,119
_RS02735
MMP_RS02740: MMP_RS02740 - hypothetical protein, at 523,200 to 523,469
_RS02740
MMP_RS02745: MMP_RS02745 - hypothetical protein, at 523,566 to 523,943
_RS02745
MMP_RS02750: MMP_RS02750 - HAD-IC family P-type ATPase, at 523,959 to 526,463
_RS02750
Position (kb) 522
523
524 Strain fitness (log2 ratio) -1
0
1 at 521.621 kb on + strand at 521.621 kb on + strand at 521.621 kb on + strand at 521.623 kb on + strand at 521.635 kb on - strand at 521.635 kb on - strand at 521.636 kb on + strand at 521.636 kb on + strand at 521.636 kb on + strand at 521.672 kb on - strand at 521.675 kb on - strand at 521.676 kb on + strand at 521.680 kb on + strand at 521.709 kb on - strand at 521.710 kb on + strand at 521.710 kb on + strand at 521.719 kb on - strand at 521.719 kb on - strand at 521.825 kb on + strand, within MMP_RS02730 at 521.876 kb on - strand, within MMP_RS02730 at 521.876 kb on - strand, within MMP_RS02730 at 521.877 kb on + strand, within MMP_RS02730 at 522.134 kb on - strand, within MMP_RS02730 at 522.143 kb on - strand, within MMP_RS02730 at 522.189 kb on - strand, within MMP_RS02730 at 522.189 kb on - strand, within MMP_RS02730 at 522.189 kb on - strand, within MMP_RS02730 at 522.190 kb on + strand, within MMP_RS02730 at 522.190 kb on + strand, within MMP_RS02730 at 522.197 kb on - strand, within MMP_RS02730 at 522.461 kb on - strand, within MMP_RS02730 at 522.469 kb on - strand, within MMP_RS02730 at 522.542 kb on - strand at 522.563 kb on - strand at 522.583 kb on - strand at 522.586 kb on + strand at 522.719 kb on + strand, within MMP_RS02735 at 522.759 kb on + strand, within MMP_RS02735 at 522.759 kb on + strand, within MMP_RS02735 at 522.811 kb on + strand, within MMP_RS02735 at 522.833 kb on + strand, within MMP_RS02735 at 522.922 kb on - strand, within MMP_RS02735 at 522.931 kb on - strand, within MMP_RS02735 at 523.013 kb on + strand, within MMP_RS02735 at 523.174 kb on + strand at 523.186 kb on - strand at 523.191 kb on - strand at 523.299 kb on - strand, within MMP_RS02740 at 523.368 kb on - strand, within MMP_RS02740 at 523.397 kb on - strand, within MMP_RS02740 at 523.414 kb on + strand, within MMP_RS02740 at 523.515 kb on - strand at 523.515 kb on - strand at 523.517 kb on - strand at 523.520 kb on - strand at 523.521 kb on + strand at 523.523 kb on + strand at 523.525 kb on - strand at 523.629 kb on + strand, within MMP_RS02745 at 523.840 kb on + strand, within MMP_RS02745
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 521,621 + -0.1 521,621 + -0.2 521,621 + -0.1 521,623 + +0.6 521,635 - +0.3 521,635 - +0.3 521,636 + -0.1 521,636 + -0.2 521,636 + -0.2 521,672 - +0.0 521,675 - -0.3 521,676 + +0.1 521,680 + -0.1 521,709 - +0.3 521,710 + -0.3 521,710 + -0.1 521,719 - +0.1 521,719 - -0.1 521,825 + MMP_RS02730 0.13 -0.2 521,876 - MMP_RS02730 0.19 -0.1 521,876 - MMP_RS02730 0.19 +0.0 521,877 + MMP_RS02730 0.20 +0.3 522,134 - MMP_RS02730 0.50 +0.2 522,143 - MMP_RS02730 0.51 -0.2 522,189 - MMP_RS02730 0.56 +0.0 522,189 - MMP_RS02730 0.56 +0.2 522,189 - MMP_RS02730 0.56 +0.5 522,190 + MMP_RS02730 0.56 -0.1 522,190 + MMP_RS02730 0.56 +0.1 522,197 - MMP_RS02730 0.57 +0.0 522,461 - MMP_RS02730 0.88 +0.2 522,469 - MMP_RS02730 0.89 -0.0 522,542 - -0.5 522,563 - +0.5 522,583 - +0.1 522,586 + +0.1 522,719 + MMP_RS02735 0.24 +0.1 522,759 + MMP_RS02735 0.32 +0.1 522,759 + MMP_RS02735 0.32 +0.2 522,811 + MMP_RS02735 0.42 +0.3 522,833 + MMP_RS02735 0.46 +0.5 522,922 - MMP_RS02735 0.63 +0.2 522,931 - MMP_RS02735 0.64 -0.2 523,013 + MMP_RS02735 0.80 -0.6 523,174 + +0.2 523,186 - +0.3 523,191 - +0.2 523,299 - MMP_RS02740 0.37 -0.3 523,368 - MMP_RS02740 0.62 +0.1 523,397 - MMP_RS02740 0.73 -0.2 523,414 + MMP_RS02740 0.79 +0.1 523,515 - +0.8 523,515 - +0.4 523,517 - +0.5 523,520 - -0.2 523,521 + +0.5 523,523 + +0.5 523,525 - +0.2 523,629 + MMP_RS02745 0.17 -0.1 523,840 + MMP_RS02745 0.72 -0.1
Or see this region's nucleotide sequence