Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01975 and MMP_RS01980 are separated by 133 nucleotides MMP_RS01980 and MMP_RS01985 are separated by 79 nucleotides MMP_RS01985 and MMP_RS01990 are separated by 203 nucleotides MMP_RS01990 and MMP_RS01995 are separated by 39 nucleotides
MMP_RS01975: MMP_RS01975 - hypothetical protein, at 366,983 to 368,389
_RS01975
MMP_RS01980: MMP_RS01980 - trimeric intracellular cation channel family protein, at 368,523 to 369,152
_RS01980
MMP_RS01985: MMP_RS01985 - F420-dependent methylenetetrahydromethanopterin dehydrogenase, at 369,232 to 370,065
_RS01985
MMP_RS01990: MMP_RS01990 - hypothetical protein, at 370,269 to 370,517
_RS01990
MMP_RS01995: MMP_RS01995 - ribonuclease VapC5, at 370,557 to 371,087
_RS01995
Position (kb)
369
370
371 Strain fitness (log2 ratio)
-2
-1
0
1 at 368.273 kb on - strand at 368.320 kb on + strand at 368.327 kb on + strand at 368.331 kb on - strand at 368.331 kb on - strand at 368.354 kb on + strand at 368.365 kb on + strand at 368.374 kb on + strand at 368.380 kb on - strand at 368.399 kb on - strand at 368.448 kb on - strand at 368.470 kb on + strand at 368.471 kb on - strand at 368.473 kb on - strand at 368.474 kb on + strand at 368.493 kb on - strand at 368.494 kb on + strand at 368.494 kb on + strand at 368.497 kb on - strand at 368.497 kb on - strand at 368.528 kb on - strand at 368.604 kb on + strand, within MMP_RS01980 at 368.615 kb on + strand, within MMP_RS01980 at 368.646 kb on + strand, within MMP_RS01980 at 368.691 kb on + strand, within MMP_RS01980 at 368.707 kb on - strand, within MMP_RS01980 at 368.788 kb on - strand, within MMP_RS01980 at 368.788 kb on - strand, within MMP_RS01980 at 368.788 kb on - strand, within MMP_RS01980 at 368.789 kb on + strand, within MMP_RS01980 at 368.789 kb on + strand, within MMP_RS01980 at 368.799 kb on - strand, within MMP_RS01980 at 368.799 kb on - strand, within MMP_RS01980 at 368.807 kb on + strand, within MMP_RS01980 at 368.865 kb on - strand, within MMP_RS01980 at 368.866 kb on + strand, within MMP_RS01980 at 368.867 kb on - strand, within MMP_RS01980 at 368.868 kb on + strand, within MMP_RS01980 at 368.894 kb on + strand, within MMP_RS01980 at 368.898 kb on + strand, within MMP_RS01980 at 368.935 kb on - strand, within MMP_RS01980 at 368.936 kb on + strand, within MMP_RS01980 at 368.936 kb on + strand, within MMP_RS01980 at 368.936 kb on + strand, within MMP_RS01980 at 368.944 kb on - strand, within MMP_RS01980 at 368.950 kb on - strand, within MMP_RS01980 at 368.986 kb on + strand, within MMP_RS01980 at 369.022 kb on + strand, within MMP_RS01980 at 369.157 kb on - strand at 369.212 kb on - strand at 369.256 kb on + strand at 369.361 kb on - strand, within MMP_RS01985 at 369.647 kb on + strand, within MMP_RS01985 at 369.647 kb on + strand, within MMP_RS01985 at 369.683 kb on + strand, within MMP_RS01985 at 369.890 kb on + strand, within MMP_RS01985 at 369.890 kb on + strand, within MMP_RS01985 at 370.067 kb on + strand at 370.067 kb on + strand at 370.067 kb on + strand at 370.100 kb on - strand at 370.120 kb on - strand at 370.121 kb on + strand at 370.178 kb on - strand at 370.202 kb on + strand at 370.202 kb on + strand at 370.215 kb on - strand at 370.223 kb on - strand at 370.318 kb on - strand, within MMP_RS01990 at 370.318 kb on - strand, within MMP_RS01990 at 370.344 kb on + strand, within MMP_RS01990 at 370.382 kb on + strand, within MMP_RS01990 at 370.516 kb on + strand at 370.554 kb on - strand at 370.555 kb on + strand at 370.555 kb on + strand at 370.591 kb on - strand at 370.592 kb on + strand at 370.811 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 368,273 - +0.1 368,320 + -0.1 368,327 + +0.4 368,331 - -0.2 368,331 - -0.3 368,354 + -0.2 368,365 + +0.3 368,374 + +0.7 368,380 - +0.1 368,399 - +0.8 368,448 - +0.0 368,470 + -0.7 368,471 - -0.8 368,473 - -0.7 368,474 + -0.8 368,493 - -0.8 368,494 + -0.3 368,494 + -0.4 368,497 - -1.0 368,497 - -1.0 368,528 - -0.5 368,604 + MMP_RS01980 0.13 -0.1 368,615 + MMP_RS01980 0.15 -1.0 368,646 + MMP_RS01980 0.20 -1.2 368,691 + MMP_RS01980 0.27 -1.0 368,707 - MMP_RS01980 0.29 -1.0 368,788 - MMP_RS01980 0.42 -0.7 368,788 - MMP_RS01980 0.42 -0.5 368,788 - MMP_RS01980 0.42 -0.9 368,789 + MMP_RS01980 0.42 -1.3 368,789 + MMP_RS01980 0.42 -1.1 368,799 - MMP_RS01980 0.44 -2.4 368,799 - MMP_RS01980 0.44 -0.4 368,807 + MMP_RS01980 0.45 -0.6 368,865 - MMP_RS01980 0.54 -0.9 368,866 + MMP_RS01980 0.54 -1.3 368,867 - MMP_RS01980 0.55 -1.5 368,868 + MMP_RS01980 0.55 -0.4 368,894 + MMP_RS01980 0.59 -0.5 368,898 + MMP_RS01980 0.60 -1.0 368,935 - MMP_RS01980 0.65 -0.7 368,936 + MMP_RS01980 0.66 -0.7 368,936 + MMP_RS01980 0.66 -0.6 368,936 + MMP_RS01980 0.66 -1.0 368,944 - MMP_RS01980 0.67 -0.5 368,950 - MMP_RS01980 0.68 -0.8 368,986 + MMP_RS01980 0.73 -1.3 369,022 + MMP_RS01980 0.79 -0.9 369,157 - +0.3 369,212 - -0.7 369,256 + -1.0 369,361 - MMP_RS01985 0.15 -1.2 369,647 + MMP_RS01985 0.50 -0.6 369,647 + MMP_RS01985 0.50 +0.3 369,683 + MMP_RS01985 0.54 -0.5 369,890 + MMP_RS01985 0.79 -0.7 369,890 + MMP_RS01985 0.79 -0.9 370,067 + -1.0 370,067 + -1.7 370,067 + -1.3 370,100 - -1.1 370,120 - -1.7 370,121 + -0.8 370,178 - +1.5 370,202 + -0.4 370,202 + -0.0 370,215 - +0.1 370,223 - +0.4 370,318 - MMP_RS01990 0.20 -0.6 370,318 - MMP_RS01990 0.20 +0.4 370,344 + MMP_RS01990 0.30 -0.1 370,382 + MMP_RS01990 0.45 -0.5 370,516 + -0.2 370,554 - +0.0 370,555 + +0.2 370,555 + -0.0 370,591 - -0.1 370,592 + -0.1 370,811 - +1.0
Or see this region's nucleotide sequence