Experiment: phosphite 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_668 and Pf6N2E2_669 are separated by 70 nucleotides Pf6N2E2_669 and Pf6N2E2_670 are separated by 137 nucleotides Pf6N2E2_670 and Pf6N2E2_671 are separated by 214 nucleotides
Pf6N2E2_668 - 2,3-butanediol dehydrogenase, R-alcohol forming, (R)- and (S)-acetoin-specific (EC 1.1.1.4), at 773,226 to 774,320
_668
Pf6N2E2_669 - Nuclease inhibitor, at 774,391 to 774,945
_669
Pf6N2E2_670 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 775,083 to 776,000
_670
Pf6N2E2_671 - 2-methylaconitate racemase, at 776,215 to 777,243
_671
Position (kb) 775
776
777 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 774.147 kb on + strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.466 kb on - strand, within Pf6N2E2_669 at 774.466 kb on - strand, within Pf6N2E2_669 at 774.746 kb on - strand, within Pf6N2E2_669 at 774.782 kb on + strand, within Pf6N2E2_669 at 774.783 kb on - strand, within Pf6N2E2_669 at 774.783 kb on - strand, within Pf6N2E2_669 at 774.858 kb on - strand, within Pf6N2E2_669 at 774.938 kb on + strand at 774.938 kb on + strand at 774.939 kb on - strand at 775.072 kb on + strand at 775.084 kb on + strand at 775.147 kb on + strand at 775.147 kb on + strand at 775.148 kb on - strand at 775.148 kb on - strand at 775.288 kb on + strand, within Pf6N2E2_670 at 775.288 kb on + strand at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.417 kb on - strand, within Pf6N2E2_670 at 775.570 kb on + strand, within Pf6N2E2_670 at 775.570 kb on + strand, within Pf6N2E2_670 at 775.571 kb on - strand, within Pf6N2E2_670 at 775.571 kb on - strand, within Pf6N2E2_670 at 775.588 kb on + strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.663 kb on + strand, within Pf6N2E2_670 at 775.663 kb on + strand, within Pf6N2E2_670 at 776.032 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.042 kb on + strand at 776.043 kb on - strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.058 kb on - strand at 776.058 kb on - strand at 776.061 kb on + strand at 776.062 kb on - strand at 776.062 kb on - strand at 776.062 kb on - strand at 776.117 kb on + strand at 776.117 kb on + strand at 776.141 kb on + strand at 776.328 kb on - strand, within Pf6N2E2_671 at 776.406 kb on + strand, within Pf6N2E2_671 at 776.406 kb on + strand, within Pf6N2E2_671 at 776.454 kb on + strand, within Pf6N2E2_671 at 776.454 kb on + strand, within Pf6N2E2_671 at 776.739 kb on - strand, within Pf6N2E2_671
Per-strain Table
Position Strand Gene LocusTag Fraction phosphite 0.25 M remove 774,147 + Pf6N2E2_668 0.84 +0.8 774,148 - Pf6N2E2_668 0.84 -1.8 774,148 - Pf6N2E2_668 0.84 -0.3 774,148 - Pf6N2E2_668 0.84 -0.3 774,466 - Pf6N2E2_669 0.14 -1.3 774,466 - Pf6N2E2_669 0.14 +0.1 774,746 - Pf6N2E2_669 0.64 +0.6 774,782 + Pf6N2E2_669 0.70 +0.5 774,783 - Pf6N2E2_669 0.71 +1.3 774,783 - Pf6N2E2_669 0.71 -0.3 774,858 - Pf6N2E2_669 0.84 -2.6 774,938 + -0.4 774,938 + +1.2 774,939 - +0.3 775,072 + -0.4 775,084 + -0.4 775,147 + +1.1 775,147 + +0.8 775,148 - -0.1 775,148 - -1.6 775,288 + Pf6N2E2_670 0.22 +0.2 775,288 + +0.3 775,289 - Pf6N2E2_670 0.22 +0.7 775,289 - Pf6N2E2_670 0.22 +1.8 775,289 - Pf6N2E2_670 0.22 -1.3 775,416 + Pf6N2E2_670 0.36 +0.6 775,416 + Pf6N2E2_670 0.36 -0.1 775,416 + Pf6N2E2_670 0.36 -1.1 775,417 - Pf6N2E2_670 0.36 -2.8 775,570 + Pf6N2E2_670 0.53 +0.3 775,570 + Pf6N2E2_670 0.53 -0.0 775,571 - Pf6N2E2_670 0.53 -0.9 775,571 - Pf6N2E2_670 0.53 -1.4 775,588 + Pf6N2E2_670 0.55 -1.7 775,589 - Pf6N2E2_670 0.55 +0.7 775,589 - Pf6N2E2_670 0.55 +0.0 775,589 - Pf6N2E2_670 0.55 +0.1 775,663 + Pf6N2E2_670 0.63 -0.1 775,663 + Pf6N2E2_670 0.63 -0.8 776,032 + -0.2 776,034 + -0.5 776,034 + -0.6 776,034 + -2.1 776,034 + -0.1 776,034 + +1.8 776,034 + +0.7 776,034 + +0.9 776,035 - +0.3 776,035 - +0.1 776,035 - +0.5 776,035 - -0.3 776,042 + +0.5 776,043 - +0.9 776,057 + +1.0 776,057 + -0.8 776,057 + +0.8 776,057 + -0.7 776,057 + -0.1 776,058 - -0.6 776,058 - +0.1 776,061 + +0.3 776,062 - -1.5 776,062 - -0.5 776,062 - +0.3 776,117 + +0.6 776,117 + -0.6 776,141 + +0.5 776,328 - Pf6N2E2_671 0.11 -0.4 776,406 + Pf6N2E2_671 0.19 -1.9 776,406 + Pf6N2E2_671 0.19 +0.2 776,454 + Pf6N2E2_671 0.23 -0.6 776,454 + Pf6N2E2_671 0.23 +0.1 776,739 - Pf6N2E2_671 0.51 +1.3
Or see this region's nucleotide sequence