Experiment: phosphite 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6077 and Pf6N2E2_6078 are separated by 45 nucleotides Pf6N2E2_6078 and Pf6N2E2_6079 are separated by 9 nucleotides Pf6N2E2_6079 and Pf6N2E2_6080 are separated by 32 nucleotides
Pf6N2E2_6077 - Cysteine desulfurase (EC 2.8.1.7), at 6,896,387 to 6,897,553
_6077
Pf6N2E2_6078 - Xaa-Pro aminopeptidase (EC 3.4.11.9), at 6,897,599 to 6,899,407
_6078
Pf6N2E2_6079 - 2-hydroxychromene-2-carboxylate isomerase family protein, glutathione-dependent, at 6,899,417 to 6,900,007
_6079
Pf6N2E2_6080 - Short-chain dehydrogenase, associated with 2-hydroxychromene-2-carboxylate isomerase family protein, at 6,900,040 to 6,900,768
_6080
Position (kb)
6897
6898
6899
6900 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6897.011 kb on + strand, within Pf6N2E2_6077 at 6897.011 kb on + strand, within Pf6N2E2_6077 at 6897.011 kb on + strand, within Pf6N2E2_6077 at 6897.011 kb on + strand, within Pf6N2E2_6077 at 6897.012 kb on - strand, within Pf6N2E2_6077 at 6897.012 kb on - strand, within Pf6N2E2_6077 at 6897.038 kb on + strand, within Pf6N2E2_6077 at 6897.038 kb on + strand, within Pf6N2E2_6077 at 6897.038 kb on + strand, within Pf6N2E2_6077 at 6897.039 kb on - strand, within Pf6N2E2_6077 at 6897.253 kb on + strand, within Pf6N2E2_6077 at 6897.253 kb on + strand, within Pf6N2E2_6077 at 6897.253 kb on + strand, within Pf6N2E2_6077 at 6897.254 kb on - strand, within Pf6N2E2_6077 at 6897.254 kb on - strand, within Pf6N2E2_6077 at 6897.254 kb on - strand, within Pf6N2E2_6077 at 6897.254 kb on - strand, within Pf6N2E2_6077 at 6897.578 kb on + strand at 6897.578 kb on + strand at 6897.579 kb on - strand at 6897.781 kb on + strand, within Pf6N2E2_6078 at 6898.094 kb on + strand, within Pf6N2E2_6078 at 6898.094 kb on + strand, within Pf6N2E2_6078 at 6898.094 kb on + strand, within Pf6N2E2_6078 at 6898.095 kb on - strand, within Pf6N2E2_6078 at 6898.095 kb on - strand, within Pf6N2E2_6078 at 6898.095 kb on - strand, within Pf6N2E2_6078 at 6898.373 kb on + strand, within Pf6N2E2_6078 at 6898.374 kb on - strand, within Pf6N2E2_6078 at 6898.821 kb on - strand, within Pf6N2E2_6078 at 6899.009 kb on + strand, within Pf6N2E2_6078 at 6899.009 kb on + strand, within Pf6N2E2_6078 at 6899.040 kb on - strand, within Pf6N2E2_6078 at 6899.078 kb on + strand, within Pf6N2E2_6078 at 6899.147 kb on + strand, within Pf6N2E2_6078 at 6899.148 kb on - strand, within Pf6N2E2_6078 at 6899.148 kb on - strand, within Pf6N2E2_6078 at 6899.148 kb on - strand, within Pf6N2E2_6078 at 6899.148 kb on - strand, within Pf6N2E2_6078 at 6899.410 kb on + strand at 6899.410 kb on + strand at 6899.580 kb on + strand, within Pf6N2E2_6079 at 6899.580 kb on + strand, within Pf6N2E2_6079 at 6899.580 kb on + strand, within Pf6N2E2_6079 at 6899.580 kb on + strand, within Pf6N2E2_6079 at 6899.581 kb on - strand, within Pf6N2E2_6079 at 6899.581 kb on - strand, within Pf6N2E2_6079 at 6899.581 kb on - strand, within Pf6N2E2_6079 at 6899.581 kb on - strand, within Pf6N2E2_6079 at 6899.581 kb on - strand, within Pf6N2E2_6079 at 6899.581 kb on - strand, within Pf6N2E2_6079 at 6899.581 kb on - strand, within Pf6N2E2_6079 at 6899.746 kb on + strand, within Pf6N2E2_6079 at 6899.746 kb on + strand, within Pf6N2E2_6079 at 6899.746 kb on + strand, within Pf6N2E2_6079 at 6899.746 kb on + strand, within Pf6N2E2_6079 at 6899.747 kb on - strand, within Pf6N2E2_6079 at 6899.747 kb on - strand, within Pf6N2E2_6079 at 6899.747 kb on - strand, within Pf6N2E2_6079 at 6899.747 kb on - strand, within Pf6N2E2_6079 at 6899.823 kb on + strand, within Pf6N2E2_6079 at 6899.823 kb on + strand, within Pf6N2E2_6079 at 6899.824 kb on - strand, within Pf6N2E2_6079 at 6899.888 kb on + strand, within Pf6N2E2_6079 at 6899.958 kb on + strand at 6899.985 kb on + strand at 6900.041 kb on + strand at 6900.042 kb on - strand at 6900.110 kb on + strand at 6900.110 kb on + strand at 6900.110 kb on + strand at 6900.116 kb on + strand, within Pf6N2E2_6080 at 6900.117 kb on - strand, within Pf6N2E2_6080 at 6900.117 kb on - strand, within Pf6N2E2_6080 at 6900.117 kb on - strand, within Pf6N2E2_6080 at 6900.117 kb on - strand, within Pf6N2E2_6080 at 6900.171 kb on - strand, within Pf6N2E2_6080 at 6900.171 kb on - strand, within Pf6N2E2_6080 at 6900.243 kb on + strand, within Pf6N2E2_6080
Per-strain Table
Position Strand Gene LocusTag Fraction phosphite 0.25 M remove 6,897,011 + Pf6N2E2_6077 0.53 +0.0 6,897,011 + Pf6N2E2_6077 0.53 +0.1 6,897,011 + Pf6N2E2_6077 0.53 +0.4 6,897,011 + Pf6N2E2_6077 0.53 -0.3 6,897,012 - Pf6N2E2_6077 0.54 -0.2 6,897,012 - Pf6N2E2_6077 0.54 -2.9 6,897,038 + Pf6N2E2_6077 0.56 +0.1 6,897,038 + Pf6N2E2_6077 0.56 -1.8 6,897,038 + Pf6N2E2_6077 0.56 -1.4 6,897,039 - Pf6N2E2_6077 0.56 +1.1 6,897,253 + Pf6N2E2_6077 0.74 -0.1 6,897,253 + Pf6N2E2_6077 0.74 +0.4 6,897,253 + Pf6N2E2_6077 0.74 -0.7 6,897,254 - Pf6N2E2_6077 0.74 -1.2 6,897,254 - Pf6N2E2_6077 0.74 -1.4 6,897,254 - Pf6N2E2_6077 0.74 +0.1 6,897,254 - Pf6N2E2_6077 0.74 +0.1 6,897,578 + -1.2 6,897,578 + +1.0 6,897,579 - +0.3 6,897,781 + Pf6N2E2_6078 0.10 -0.0 6,898,094 + Pf6N2E2_6078 0.27 +0.4 6,898,094 + Pf6N2E2_6078 0.27 +0.9 6,898,094 + Pf6N2E2_6078 0.27 -1.3 6,898,095 - Pf6N2E2_6078 0.27 -1.0 6,898,095 - Pf6N2E2_6078 0.27 -0.2 6,898,095 - Pf6N2E2_6078 0.27 -0.4 6,898,373 + Pf6N2E2_6078 0.43 -1.7 6,898,374 - Pf6N2E2_6078 0.43 -2.0 6,898,821 - Pf6N2E2_6078 0.68 +0.3 6,899,009 + Pf6N2E2_6078 0.78 +0.2 6,899,009 + Pf6N2E2_6078 0.78 -0.4 6,899,040 - Pf6N2E2_6078 0.80 +1.6 6,899,078 + Pf6N2E2_6078 0.82 -0.2 6,899,147 + Pf6N2E2_6078 0.86 -0.4 6,899,148 - Pf6N2E2_6078 0.86 -0.1 6,899,148 - Pf6N2E2_6078 0.86 -1.0 6,899,148 - Pf6N2E2_6078 0.86 -0.4 6,899,148 - Pf6N2E2_6078 0.86 +0.1 6,899,410 + +0.6 6,899,410 + +0.2 6,899,580 + Pf6N2E2_6079 0.28 -1.3 6,899,580 + Pf6N2E2_6079 0.28 +0.5 6,899,580 + Pf6N2E2_6079 0.28 +0.1 6,899,580 + Pf6N2E2_6079 0.28 +0.6 6,899,581 - Pf6N2E2_6079 0.28 -0.6 6,899,581 - Pf6N2E2_6079 0.28 +1.3 6,899,581 - Pf6N2E2_6079 0.28 -0.1 6,899,581 - Pf6N2E2_6079 0.28 +0.9 6,899,581 - Pf6N2E2_6079 0.28 +0.7 6,899,581 - Pf6N2E2_6079 0.28 +0.0 6,899,581 - Pf6N2E2_6079 0.28 +1.8 6,899,746 + Pf6N2E2_6079 0.56 -2.0 6,899,746 + Pf6N2E2_6079 0.56 +0.0 6,899,746 + Pf6N2E2_6079 0.56 +1.4 6,899,746 + Pf6N2E2_6079 0.56 -1.0 6,899,747 - Pf6N2E2_6079 0.56 -0.7 6,899,747 - Pf6N2E2_6079 0.56 -0.4 6,899,747 - Pf6N2E2_6079 0.56 -0.4 6,899,747 - Pf6N2E2_6079 0.56 +0.1 6,899,823 + Pf6N2E2_6079 0.69 +0.1 6,899,823 + Pf6N2E2_6079 0.69 +2.6 6,899,824 - Pf6N2E2_6079 0.69 -0.2 6,899,888 + Pf6N2E2_6079 0.80 -1.7 6,899,958 + -0.2 6,899,985 + +0.3 6,900,041 + -2.9 6,900,042 - +1.0 6,900,110 + +0.8 6,900,110 + +0.3 6,900,110 + -0.5 6,900,116 + Pf6N2E2_6080 0.10 -0.2 6,900,117 - Pf6N2E2_6080 0.11 -0.1 6,900,117 - Pf6N2E2_6080 0.11 +1.1 6,900,117 - Pf6N2E2_6080 0.11 +0.3 6,900,117 - Pf6N2E2_6080 0.11 +2.2 6,900,171 - Pf6N2E2_6080 0.18 +0.1 6,900,171 - Pf6N2E2_6080 0.18 +0.8 6,900,243 + Pf6N2E2_6080 0.28 -2.4
Or see this region's nucleotide sequence