Experiment: phosphite 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2542 and Pf6N2E2_2543 are separated by 3 nucleotides Pf6N2E2_2543 and Pf6N2E2_2544 are separated by 79 nucleotides
Pf6N2E2_2542 - Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4), at 3,002,566 to 3,004,338
_2542
Pf6N2E2_2543 - Cyanophycin synthase (EC 6.3.2.29)(EC 6.3.2.30), at 3,004,342 to 3,006,087
_2543
Pf6N2E2_2544 - Deblocking aminopeptidase (EC 3.4.11.-) @ Cyanophycinase 2 (EC 3.4.15.6), at 3,006,167 to 3,007,357
_2544
Position (kb)
3004
3005
3006
3007 Strain fitness (log2 ratio)
-1
0
1
2 at 3003.359 kb on + strand, within Pf6N2E2_2542 at 3003.360 kb on - strand, within Pf6N2E2_2542 at 3003.360 kb on - strand, within Pf6N2E2_2542 at 3003.419 kb on + strand, within Pf6N2E2_2542 at 3003.511 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.629 kb on + strand, within Pf6N2E2_2542 at 3003.629 kb on + strand, within Pf6N2E2_2542 at 3003.630 kb on - strand, within Pf6N2E2_2542 at 3003.630 kb on - strand, within Pf6N2E2_2542 at 3003.787 kb on + strand, within Pf6N2E2_2542 at 3003.788 kb on - strand, within Pf6N2E2_2542 at 3003.788 kb on - strand, within Pf6N2E2_2542 at 3003.788 kb on - strand, within Pf6N2E2_2542 at 3003.884 kb on + strand, within Pf6N2E2_2542 at 3004.064 kb on + strand, within Pf6N2E2_2542 at 3004.065 kb on - strand, within Pf6N2E2_2542 at 3004.065 kb on - strand, within Pf6N2E2_2542 at 3004.123 kb on + strand, within Pf6N2E2_2542 at 3004.124 kb on - strand, within Pf6N2E2_2542 at 3004.399 kb on + strand at 3004.399 kb on + strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.568 kb on + strand, within Pf6N2E2_2543 at 3004.569 kb on - strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.680 kb on - strand, within Pf6N2E2_2543 at 3004.744 kb on + strand, within Pf6N2E2_2543 at 3004.744 kb on + strand, within Pf6N2E2_2543 at 3004.745 kb on - strand, within Pf6N2E2_2543 at 3004.745 kb on - strand, within Pf6N2E2_2543 at 3005.011 kb on + strand, within Pf6N2E2_2543 at 3005.011 kb on + strand, within Pf6N2E2_2543 at 3005.012 kb on - strand, within Pf6N2E2_2543 at 3005.012 kb on - strand, within Pf6N2E2_2543 at 3005.213 kb on + strand, within Pf6N2E2_2543 at 3005.214 kb on - strand, within Pf6N2E2_2543 at 3005.214 kb on - strand, within Pf6N2E2_2543 at 3005.214 kb on - strand, within Pf6N2E2_2543 at 3005.214 kb on - strand, within Pf6N2E2_2543 at 3005.219 kb on + strand, within Pf6N2E2_2543 at 3005.220 kb on - strand, within Pf6N2E2_2543 at 3005.727 kb on + strand, within Pf6N2E2_2543 at 3005.727 kb on + strand, within Pf6N2E2_2543 at 3005.782 kb on + strand, within Pf6N2E2_2543 at 3005.977 kb on + strand at 3005.978 kb on - strand at 3006.086 kb on - strand at 3006.145 kb on + strand at 3006.145 kb on + strand at 3006.145 kb on + strand at 3006.145 kb on + strand at 3006.145 kb on + strand at 3006.145 kb on + strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.201 kb on - strand at 3006.201 kb on - strand at 3006.272 kb on + strand at 3006.272 kb on + strand at 3006.273 kb on - strand at 3006.623 kb on + strand, within Pf6N2E2_2544 at 3006.674 kb on + strand, within Pf6N2E2_2544 at 3006.674 kb on + strand, within Pf6N2E2_2544 at 3006.675 kb on - strand, within Pf6N2E2_2544 at 3006.675 kb on - strand, within Pf6N2E2_2544 at 3006.675 kb on - strand, within Pf6N2E2_2544 at 3006.830 kb on - strand, within Pf6N2E2_2544 at 3006.981 kb on - strand, within Pf6N2E2_2544 at 3007.058 kb on + strand, within Pf6N2E2_2544 at 3007.059 kb on - strand, within Pf6N2E2_2544 at 3007.061 kb on + strand, within Pf6N2E2_2544 at 3007.062 kb on - strand, within Pf6N2E2_2544 at 3007.062 kb on - strand, within Pf6N2E2_2544
Per-strain Table
Position Strand Gene LocusTag Fraction phosphite 0.25 M remove 3,003,359 + Pf6N2E2_2542 0.45 +0.7 3,003,360 - Pf6N2E2_2542 0.45 +1.3 3,003,360 - Pf6N2E2_2542 0.45 +1.5 3,003,419 + Pf6N2E2_2542 0.48 +2.2 3,003,511 + Pf6N2E2_2542 0.53 +1.7 3,003,625 + Pf6N2E2_2542 0.60 +1.5 3,003,625 + Pf6N2E2_2542 0.60 +0.1 3,003,625 + Pf6N2E2_2542 0.60 +0.6 3,003,625 + Pf6N2E2_2542 0.60 +0.7 3,003,625 + Pf6N2E2_2542 0.60 +0.1 3,003,625 + Pf6N2E2_2542 0.60 +1.5 3,003,625 + Pf6N2E2_2542 0.60 -0.7 3,003,626 - Pf6N2E2_2542 0.60 -0.3 3,003,626 - Pf6N2E2_2542 0.60 +0.1 3,003,626 - Pf6N2E2_2542 0.60 +1.3 3,003,626 - Pf6N2E2_2542 0.60 +0.0 3,003,626 - Pf6N2E2_2542 0.60 +1.1 3,003,626 - Pf6N2E2_2542 0.60 +0.5 3,003,626 - Pf6N2E2_2542 0.60 +2.4 3,003,626 - Pf6N2E2_2542 0.60 +1.3 3,003,626 - Pf6N2E2_2542 0.60 +1.9 3,003,629 + Pf6N2E2_2542 0.60 +0.1 3,003,629 + Pf6N2E2_2542 0.60 +0.7 3,003,630 - Pf6N2E2_2542 0.60 +0.1 3,003,630 - Pf6N2E2_2542 0.60 +1.7 3,003,787 + Pf6N2E2_2542 0.69 +2.3 3,003,788 - Pf6N2E2_2542 0.69 +0.8 3,003,788 - Pf6N2E2_2542 0.69 +1.7 3,003,788 - Pf6N2E2_2542 0.69 +0.3 3,003,884 + Pf6N2E2_2542 0.74 -0.7 3,004,064 + Pf6N2E2_2542 0.84 +1.3 3,004,065 - Pf6N2E2_2542 0.85 -0.1 3,004,065 - Pf6N2E2_2542 0.85 -0.2 3,004,123 + Pf6N2E2_2542 0.88 +0.4 3,004,124 - Pf6N2E2_2542 0.88 +1.4 3,004,399 + +1.2 3,004,399 + -1.1 3,004,400 - +0.4 3,004,400 - -0.6 3,004,400 - +0.8 3,004,400 - +0.6 3,004,400 - +0.3 3,004,400 - +0.3 3,004,400 - +1.4 3,004,568 + Pf6N2E2_2543 0.13 +1.7 3,004,569 - Pf6N2E2_2543 0.13 -0.1 3,004,570 + Pf6N2E2_2543 0.13 +0.3 3,004,570 + Pf6N2E2_2543 0.13 +1.6 3,004,570 + Pf6N2E2_2543 0.13 +1.9 3,004,570 + Pf6N2E2_2543 0.13 +1.9 3,004,570 + Pf6N2E2_2543 0.13 +1.2 3,004,570 + Pf6N2E2_2543 0.13 -0.2 3,004,570 + Pf6N2E2_2543 0.13 +0.4 3,004,570 + Pf6N2E2_2543 0.13 +0.6 3,004,570 + Pf6N2E2_2543 0.13 +1.1 3,004,571 - Pf6N2E2_2543 0.13 -1.1 3,004,571 - Pf6N2E2_2543 0.13 -0.2 3,004,571 - Pf6N2E2_2543 0.13 +0.9 3,004,571 - Pf6N2E2_2543 0.13 +0.1 3,004,571 - Pf6N2E2_2543 0.13 +1.6 3,004,571 - Pf6N2E2_2543 0.13 +2.0 3,004,571 - Pf6N2E2_2543 0.13 +1.8 3,004,571 - Pf6N2E2_2543 0.13 +0.9 3,004,571 - Pf6N2E2_2543 0.13 +1.2 3,004,571 - Pf6N2E2_2543 0.13 +0.2 3,004,680 - Pf6N2E2_2543 0.19 +2.2 3,004,744 + Pf6N2E2_2543 0.23 +0.7 3,004,744 + Pf6N2E2_2543 0.23 -0.0 3,004,745 - Pf6N2E2_2543 0.23 +2.0 3,004,745 - Pf6N2E2_2543 0.23 +0.9 3,005,011 + Pf6N2E2_2543 0.38 -0.7 3,005,011 + Pf6N2E2_2543 0.38 +1.5 3,005,012 - Pf6N2E2_2543 0.38 +1.4 3,005,012 - Pf6N2E2_2543 0.38 +0.9 3,005,213 + Pf6N2E2_2543 0.50 +0.9 3,005,214 - Pf6N2E2_2543 0.50 +1.4 3,005,214 - Pf6N2E2_2543 0.50 +0.1 3,005,214 - Pf6N2E2_2543 0.50 +1.3 3,005,214 - Pf6N2E2_2543 0.50 +2.5 3,005,219 + Pf6N2E2_2543 0.50 +0.7 3,005,220 - Pf6N2E2_2543 0.50 +1.3 3,005,727 + Pf6N2E2_2543 0.79 +0.1 3,005,727 + Pf6N2E2_2543 0.79 +1.8 3,005,782 + Pf6N2E2_2543 0.82 +2.0 3,005,977 + +1.5 3,005,978 - +1.3 3,006,086 - -0.1 3,006,145 + +0.3 3,006,145 + +1.9 3,006,145 + -0.4 3,006,145 + -1.1 3,006,145 + +0.1 3,006,145 + +0.8 3,006,146 - -0.8 3,006,146 - +0.7 3,006,146 - +1.3 3,006,146 - -0.5 3,006,146 - +0.2 3,006,146 - -0.1 3,006,146 - +1.0 3,006,201 - +0.2 3,006,201 - -0.6 3,006,272 + -1.1 3,006,272 + +0.7 3,006,273 - -0.3 3,006,623 + Pf6N2E2_2544 0.38 +0.7 3,006,674 + Pf6N2E2_2544 0.43 -1.5 3,006,674 + Pf6N2E2_2544 0.43 -0.8 3,006,675 - Pf6N2E2_2544 0.43 +0.8 3,006,675 - Pf6N2E2_2544 0.43 -0.6 3,006,675 - Pf6N2E2_2544 0.43 -0.2 3,006,830 - Pf6N2E2_2544 0.56 -0.0 3,006,981 - Pf6N2E2_2544 0.68 +0.2 3,007,058 + Pf6N2E2_2544 0.75 +0.8 3,007,059 - Pf6N2E2_2544 0.75 -0.0 3,007,061 + Pf6N2E2_2544 0.75 +1.3 3,007,062 - Pf6N2E2_2544 0.75 +1.1 3,007,062 - Pf6N2E2_2544 0.75 -1.0
Or see this region's nucleotide sequence