Experiment: phosphite 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2035 and Pf6N2E2_2036 are separated by 10 nucleotides Pf6N2E2_2036 and Pf6N2E2_2037 are separated by 56 nucleotides Pf6N2E2_2037 and Pf6N2E2_2038 are separated by 105 nucleotides Pf6N2E2_2038 and Pf6N2E2_2039 overlap by 1 nucleotides
Pf6N2E2_2035 - epoxide hydrolase, at 2,424,362 to 2,425,366
_2035
Pf6N2E2_2036 - Antibiotic biosynthesis monooxygenase, at 2,425,377 to 2,425,754
_2036
Pf6N2E2_2037 - Thioredoxin reductase (EC 1.8.1.9), at 2,425,811 to 2,426,704
_2037
Pf6N2E2_2038 - Transcriptional regulator, TetR family, at 2,426,810 to 2,427,469
_2038
Pf6N2E2_2039 - Sigma-54 dependent transcriptional regulator, at 2,427,469 to 2,428,884
_2039
Position (kb)
2425
2426
2427 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.889 kb on - strand, within Pf6N2E2_2035 at 2424.889 kb on - strand, within Pf6N2E2_2035 at 2425.345 kb on - strand at 2425.345 kb on - strand at 2425.367 kb on - strand at 2425.367 kb on - strand at 2425.447 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.668 kb on - strand, within Pf6N2E2_2036 at 2425.671 kb on + strand, within Pf6N2E2_2036 at 2425.671 kb on + strand, within Pf6N2E2_2036 at 2425.671 kb on + strand, within Pf6N2E2_2036 at 2425.672 kb on - strand, within Pf6N2E2_2036 at 2425.718 kb on + strand at 2425.726 kb on - strand at 2425.726 kb on - strand at 2425.915 kb on - strand, within Pf6N2E2_2037 at 2425.915 kb on - strand, within Pf6N2E2_2037 at 2426.229 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.348 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.677 kb on + strand at 2426.869 kb on + strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.872 kb on - strand at 2426.872 kb on - strand at 2427.197 kb on + strand, within Pf6N2E2_2038 at 2427.197 kb on + strand, within Pf6N2E2_2038 at 2427.215 kb on + strand, within Pf6N2E2_2038 at 2427.215 kb on + strand, within Pf6N2E2_2038 at 2427.216 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.496 kb on + strand at 2427.496 kb on + strand at 2427.610 kb on + strand at 2427.610 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction phosphite 0.25 M remove 2,424,888 + Pf6N2E2_2035 0.52 -0.4 2,424,888 + Pf6N2E2_2035 0.52 +0.4 2,424,888 + Pf6N2E2_2035 0.52 -0.2 2,424,888 + Pf6N2E2_2035 0.52 +1.0 2,424,889 - Pf6N2E2_2035 0.52 -1.0 2,424,889 - Pf6N2E2_2035 0.52 -2.3 2,425,345 - -0.6 2,425,345 - +1.4 2,425,367 - +0.8 2,425,367 - -1.6 2,425,447 + Pf6N2E2_2036 0.19 +1.1 2,425,522 + Pf6N2E2_2036 0.38 +0.4 2,425,522 + +0.4 2,425,522 + Pf6N2E2_2036 0.38 +2.0 2,425,522 + Pf6N2E2_2036 0.38 +0.5 2,425,522 + Pf6N2E2_2036 0.38 +0.5 2,425,522 + Pf6N2E2_2036 0.38 +1.5 2,425,522 + Pf6N2E2_2036 0.38 -0.1 2,425,522 + Pf6N2E2_2036 0.38 -0.0 2,425,523 - Pf6N2E2_2036 0.39 -1.2 2,425,523 - Pf6N2E2_2036 0.39 -0.5 2,425,523 - Pf6N2E2_2036 0.39 +0.5 2,425,523 - Pf6N2E2_2036 0.39 -0.6 2,425,523 - Pf6N2E2_2036 0.39 +0.2 2,425,659 + Pf6N2E2_2036 0.75 -0.9 2,425,659 + Pf6N2E2_2036 0.75 +0.8 2,425,659 + Pf6N2E2_2036 0.75 -1.7 2,425,659 + Pf6N2E2_2036 0.75 +1.0 2,425,659 + Pf6N2E2_2036 0.75 +1.1 2,425,659 + Pf6N2E2_2036 0.75 -1.4 2,425,660 - Pf6N2E2_2036 0.75 +0.5 2,425,660 - Pf6N2E2_2036 0.75 +0.3 2,425,660 - Pf6N2E2_2036 0.75 +0.1 2,425,660 - Pf6N2E2_2036 0.75 +0.7 2,425,660 - Pf6N2E2_2036 0.75 +0.0 2,425,667 + Pf6N2E2_2036 0.77 -0.1 2,425,667 + Pf6N2E2_2036 0.77 +0.5 2,425,667 + Pf6N2E2_2036 0.77 +0.4 2,425,667 + Pf6N2E2_2036 0.77 -2.5 2,425,667 + Pf6N2E2_2036 0.77 -0.8 2,425,667 + Pf6N2E2_2036 0.77 -0.2 2,425,668 - Pf6N2E2_2036 0.77 +1.4 2,425,671 + Pf6N2E2_2036 0.78 +2.6 2,425,671 + Pf6N2E2_2036 0.78 -0.1 2,425,671 + Pf6N2E2_2036 0.78 +1.2 2,425,672 - Pf6N2E2_2036 0.78 +0.6 2,425,718 + +0.3 2,425,726 - +3.7 2,425,726 - +1.4 2,425,915 - Pf6N2E2_2037 0.12 -2.7 2,425,915 - Pf6N2E2_2037 0.12 +0.1 2,426,229 + Pf6N2E2_2037 0.47 +0.3 2,426,241 + Pf6N2E2_2037 0.48 -0.5 2,426,241 + Pf6N2E2_2037 0.48 +2.4 2,426,241 + Pf6N2E2_2037 0.48 -0.5 2,426,241 + Pf6N2E2_2037 0.48 +0.0 2,426,241 + Pf6N2E2_2037 0.48 -0.7 2,426,242 - Pf6N2E2_2037 0.48 -0.0 2,426,242 - Pf6N2E2_2037 0.48 -1.1 2,426,242 - Pf6N2E2_2037 0.48 -0.4 2,426,348 - Pf6N2E2_2037 0.60 -0.1 2,426,386 - Pf6N2E2_2037 0.64 -1.1 2,426,386 - Pf6N2E2_2037 0.64 -1.1 2,426,386 - Pf6N2E2_2037 0.64 +0.4 2,426,386 - Pf6N2E2_2037 0.64 +0.1 2,426,386 - Pf6N2E2_2037 0.64 +0.0 2,426,677 + -0.5 2,426,869 + -1.2 2,426,870 - -0.0 2,426,870 - -1.5 2,426,870 - +1.0 2,426,870 - +0.7 2,426,870 - +0.2 2,426,872 - -0.1 2,426,872 - -1.2 2,427,197 + Pf6N2E2_2038 0.59 +0.0 2,427,197 + Pf6N2E2_2038 0.59 -1.1 2,427,215 + Pf6N2E2_2038 0.61 -0.9 2,427,215 + Pf6N2E2_2038 0.61 +1.1 2,427,216 - Pf6N2E2_2038 0.62 -0.5 2,427,393 - Pf6N2E2_2038 0.88 -0.9 2,427,393 - Pf6N2E2_2038 0.88 -0.9 2,427,393 - Pf6N2E2_2038 0.88 -0.6 2,427,393 - Pf6N2E2_2038 0.88 +0.2 2,427,496 + +0.6 2,427,496 + -0.1 2,427,610 + +0.9 2,427,610 + +0.9
Or see this region's nucleotide sequence