Experiment: phosphite 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1454 and Pf6N2E2_1455 are separated by 147 nucleotides Pf6N2E2_1455 and Pf6N2E2_1456 are separated by 185 nucleotides
Pf6N2E2_1454 - Xylose isomerase (EC 5.3.1.5), at 1,701,795 to 1,703,111
_1454
Pf6N2E2_1455 - Xylose ABC transporter, periplasmic xylose-binding protein XylF, at 1,703,259 to 1,704,260
_1455
Pf6N2E2_1456 - D-xylose transport ATP-binding protein XylG, at 1,704,446 to 1,706,002
_1456
Position (kb)
1703
1704
1705 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1702.344 kb on + strand, within Pf6N2E2_1454 at 1702.344 kb on + strand, within Pf6N2E2_1454 at 1702.344 kb on + strand, within Pf6N2E2_1454 at 1702.524 kb on + strand, within Pf6N2E2_1454 at 1702.525 kb on - strand, within Pf6N2E2_1454 at 1702.622 kb on + strand, within Pf6N2E2_1454 at 1702.623 kb on - strand, within Pf6N2E2_1454 at 1702.758 kb on + strand, within Pf6N2E2_1454 at 1702.759 kb on - strand, within Pf6N2E2_1454 at 1703.099 kb on + strand at 1703.099 kb on + strand at 1703.100 kb on - strand at 1703.100 kb on - strand at 1703.103 kb on + strand at 1703.103 kb on + strand at 1703.103 kb on + strand at 1703.104 kb on - strand at 1703.144 kb on + strand at 1703.145 kb on - strand at 1703.145 kb on - strand at 1703.145 kb on - strand at 1703.145 kb on - strand at 1703.189 kb on + strand at 1703.198 kb on - strand at 1703.198 kb on - strand at 1703.242 kb on + strand at 1703.243 kb on - strand at 1703.243 kb on - strand at 1703.397 kb on + strand, within Pf6N2E2_1455 at 1703.397 kb on + strand, within Pf6N2E2_1455 at 1703.398 kb on - strand, within Pf6N2E2_1455 at 1703.398 kb on - strand, within Pf6N2E2_1455 at 1703.398 kb on - strand, within Pf6N2E2_1455 at 1703.595 kb on + strand, within Pf6N2E2_1455 at 1703.596 kb on - strand, within Pf6N2E2_1455 at 1703.631 kb on + strand, within Pf6N2E2_1455 at 1703.631 kb on + strand, within Pf6N2E2_1455 at 1703.632 kb on - strand, within Pf6N2E2_1455 at 1703.706 kb on + strand, within Pf6N2E2_1455 at 1703.706 kb on + strand, within Pf6N2E2_1455 at 1704.052 kb on - strand, within Pf6N2E2_1455 at 1704.139 kb on - strand, within Pf6N2E2_1455 at 1704.139 kb on - strand, within Pf6N2E2_1455 at 1704.232 kb on - strand at 1704.399 kb on + strand at 1704.400 kb on - strand at 1704.414 kb on + strand at 1704.414 kb on + strand at 1704.414 kb on + strand at 1704.415 kb on - strand at 1705.043 kb on + strand, within Pf6N2E2_1456 at 1705.043 kb on + strand, within Pf6N2E2_1456 at 1705.044 kb on - strand, within Pf6N2E2_1456 at 1705.044 kb on - strand, within Pf6N2E2_1456 at 1705.253 kb on + strand, within Pf6N2E2_1456
Per-strain Table
Position Strand Gene LocusTag Fraction phosphite 0.25 M remove 1,702,344 + Pf6N2E2_1454 0.42 -0.2 1,702,344 + Pf6N2E2_1454 0.42 +0.9 1,702,344 + Pf6N2E2_1454 0.42 -2.0 1,702,524 + Pf6N2E2_1454 0.55 -0.7 1,702,525 - Pf6N2E2_1454 0.55 +0.1 1,702,622 + Pf6N2E2_1454 0.63 -0.2 1,702,623 - Pf6N2E2_1454 0.63 -0.2 1,702,758 + Pf6N2E2_1454 0.73 -0.7 1,702,759 - Pf6N2E2_1454 0.73 +0.6 1,703,099 + +0.3 1,703,099 + +0.1 1,703,100 - +0.0 1,703,100 - -2.4 1,703,103 + -1.3 1,703,103 + +1.5 1,703,103 + -1.9 1,703,104 - -1.4 1,703,144 + -0.1 1,703,145 - +0.1 1,703,145 - +1.4 1,703,145 - -0.0 1,703,145 - +0.1 1,703,189 + +2.2 1,703,198 - -1.0 1,703,198 - -2.7 1,703,242 + -0.1 1,703,243 - -0.8 1,703,243 - +0.8 1,703,397 + Pf6N2E2_1455 0.14 +0.0 1,703,397 + Pf6N2E2_1455 0.14 +1.3 1,703,398 - Pf6N2E2_1455 0.14 +1.4 1,703,398 - Pf6N2E2_1455 0.14 +0.4 1,703,398 - Pf6N2E2_1455 0.14 +0.7 1,703,595 + Pf6N2E2_1455 0.34 +0.3 1,703,596 - Pf6N2E2_1455 0.34 +1.2 1,703,631 + Pf6N2E2_1455 0.37 +2.3 1,703,631 + Pf6N2E2_1455 0.37 +1.5 1,703,632 - Pf6N2E2_1455 0.37 +0.8 1,703,706 + Pf6N2E2_1455 0.45 -1.1 1,703,706 + Pf6N2E2_1455 0.45 -0.0 1,704,052 - Pf6N2E2_1455 0.79 +2.0 1,704,139 - Pf6N2E2_1455 0.88 +1.8 1,704,139 - Pf6N2E2_1455 0.88 +0.9 1,704,232 - -0.1 1,704,399 + -0.1 1,704,400 - -1.7 1,704,414 + +1.0 1,704,414 + -1.7 1,704,414 + +0.7 1,704,415 - -1.2 1,705,043 + Pf6N2E2_1456 0.38 -2.4 1,705,043 + Pf6N2E2_1456 0.38 +1.2 1,705,044 - Pf6N2E2_1456 0.38 -0.3 1,705,044 - Pf6N2E2_1456 0.38 -1.7 1,705,253 + Pf6N2E2_1456 0.52 -0.0
Or see this region's nucleotide sequence