Experiment: phosphite 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1066 and Pf6N2E2_1067 are separated by 19 nucleotides Pf6N2E2_1067 and Pf6N2E2_1068 are separated by 196 nucleotides
Pf6N2E2_1066 - Permease of the drug/metabolite transporter (DMT) superfamily, at 1,243,102 to 1,243,995
_1066
Pf6N2E2_1067 - Glyceraldehyde-3-phosphate dehydrogenase, putative, at 1,244,015 to 1,245,640
_1067
Pf6N2E2_1068 - outer membrane autotransporter, at 1,245,837 to 1,246,751
_1068
Position (kb)
1244
1245
1246 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1243.071 kb on + strand at 1243.072 kb on - strand at 1243.115 kb on + strand at 1243.116 kb on - strand at 1243.116 kb on - strand at 1243.317 kb on - strand, within Pf6N2E2_1066 at 1243.402 kb on + strand, within Pf6N2E2_1066 at 1243.402 kb on + strand, within Pf6N2E2_1066 at 1243.402 kb on + strand, within Pf6N2E2_1066 at 1243.403 kb on - strand, within Pf6N2E2_1066 at 1243.555 kb on + strand, within Pf6N2E2_1066 at 1243.555 kb on + strand, within Pf6N2E2_1066 at 1243.555 kb on + strand, within Pf6N2E2_1066 at 1243.555 kb on + strand, within Pf6N2E2_1066 at 1243.556 kb on - strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.780 kb on + strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.781 kb on - strand, within Pf6N2E2_1066 at 1243.792 kb on + strand, within Pf6N2E2_1066 at 1243.792 kb on + strand, within Pf6N2E2_1066 at 1243.802 kb on + strand, within Pf6N2E2_1066 at 1243.846 kb on + strand, within Pf6N2E2_1066 at 1244.025 kb on + strand at 1244.025 kb on + strand at 1244.025 kb on + strand at 1244.025 kb on + strand at 1244.025 kb on + strand at 1244.026 kb on - strand at 1244.026 kb on - strand at 1244.026 kb on - strand at 1244.159 kb on - strand at 1244.330 kb on + strand, within Pf6N2E2_1067 at 1244.330 kb on + strand, within Pf6N2E2_1067 at 1244.330 kb on + strand, within Pf6N2E2_1067 at 1244.334 kb on + strand, within Pf6N2E2_1067 at 1244.362 kb on - strand, within Pf6N2E2_1067 at 1244.404 kb on - strand, within Pf6N2E2_1067 at 1244.404 kb on - strand, within Pf6N2E2_1067 at 1244.532 kb on + strand, within Pf6N2E2_1067 at 1244.532 kb on + strand, within Pf6N2E2_1067 at 1244.533 kb on - strand, within Pf6N2E2_1067 at 1244.858 kb on - strand, within Pf6N2E2_1067 at 1244.923 kb on - strand, within Pf6N2E2_1067 at 1244.923 kb on - strand, within Pf6N2E2_1067 at 1245.075 kb on + strand, within Pf6N2E2_1067 at 1245.075 kb on + strand, within Pf6N2E2_1067 at 1245.081 kb on + strand, within Pf6N2E2_1067 at 1245.082 kb on - strand, within Pf6N2E2_1067 at 1245.082 kb on - strand, within Pf6N2E2_1067 at 1245.208 kb on - strand, within Pf6N2E2_1067 at 1245.381 kb on + strand, within Pf6N2E2_1067 at 1245.382 kb on - strand, within Pf6N2E2_1067 at 1245.546 kb on + strand at 1245.546 kb on + strand at 1245.547 kb on - strand at 1245.687 kb on + strand at 1245.689 kb on + strand at 1245.689 kb on + strand at 1245.689 kb on + strand at 1245.690 kb on - strand at 1245.700 kb on + strand at 1245.706 kb on + strand at 1245.706 kb on + strand at 1245.706 kb on + strand at 1245.706 kb on + strand at 1245.707 kb on - strand at 1245.708 kb on + strand at 1245.761 kb on + strand at 1245.761 kb on + strand at 1245.762 kb on - strand at 1245.762 kb on - strand at 1245.867 kb on - strand at 1245.908 kb on - strand at 1245.922 kb on + strand at 1245.923 kb on - strand at 1245.923 kb on - strand at 1245.923 kb on - strand at 1245.923 kb on - strand at 1245.923 kb on - strand at 1246.069 kb on + strand, within Pf6N2E2_1068 at 1246.070 kb on - strand, within Pf6N2E2_1068 at 1246.070 kb on - strand, within Pf6N2E2_1068 at 1246.099 kb on + strand, within Pf6N2E2_1068 at 1246.099 kb on + strand, within Pf6N2E2_1068 at 1246.100 kb on - strand, within Pf6N2E2_1068 at 1246.100 kb on - strand, within Pf6N2E2_1068 at 1246.100 kb on - strand, within Pf6N2E2_1068 at 1246.100 kb on - strand, within Pf6N2E2_1068 at 1246.103 kb on + strand, within Pf6N2E2_1068 at 1246.104 kb on - strand, within Pf6N2E2_1068 at 1246.104 kb on - strand, within Pf6N2E2_1068 at 1246.104 kb on - strand, within Pf6N2E2_1068 at 1246.325 kb on - strand, within Pf6N2E2_1068 at 1246.627 kb on + strand, within Pf6N2E2_1068
Per-strain Table
Position Strand Gene LocusTag Fraction phosphite 0.25 M remove 1,243,071 + +0.3 1,243,072 - -0.2 1,243,115 + +0.2 1,243,116 - -0.2 1,243,116 - -0.4 1,243,317 - Pf6N2E2_1066 0.24 -0.1 1,243,402 + Pf6N2E2_1066 0.34 +0.8 1,243,402 + Pf6N2E2_1066 0.34 -0.4 1,243,402 + Pf6N2E2_1066 0.34 -0.0 1,243,403 - Pf6N2E2_1066 0.34 +0.0 1,243,555 + Pf6N2E2_1066 0.51 +0.5 1,243,555 + Pf6N2E2_1066 0.51 -0.3 1,243,555 + Pf6N2E2_1066 0.51 -0.7 1,243,555 + Pf6N2E2_1066 0.51 -1.2 1,243,556 - Pf6N2E2_1066 0.51 -0.8 1,243,780 + Pf6N2E2_1066 0.76 -0.8 1,243,780 + Pf6N2E2_1066 0.76 -0.4 1,243,780 + Pf6N2E2_1066 0.76 -0.8 1,243,780 + Pf6N2E2_1066 0.76 +0.4 1,243,780 + Pf6N2E2_1066 0.76 +0.3 1,243,780 + Pf6N2E2_1066 0.76 -0.5 1,243,780 + Pf6N2E2_1066 0.76 +0.3 1,243,780 + Pf6N2E2_1066 0.76 -1.2 1,243,780 + Pf6N2E2_1066 0.76 +0.8 1,243,780 + Pf6N2E2_1066 0.76 +0.0 1,243,780 + Pf6N2E2_1066 0.76 -0.1 1,243,780 + Pf6N2E2_1066 0.76 +0.8 1,243,780 + Pf6N2E2_1066 0.76 -0.7 1,243,781 - Pf6N2E2_1066 0.76 -2.4 1,243,781 - Pf6N2E2_1066 0.76 -0.0 1,243,781 - Pf6N2E2_1066 0.76 -2.2 1,243,781 - Pf6N2E2_1066 0.76 +1.0 1,243,781 - Pf6N2E2_1066 0.76 -0.2 1,243,781 - Pf6N2E2_1066 0.76 -0.6 1,243,781 - Pf6N2E2_1066 0.76 -0.8 1,243,781 - Pf6N2E2_1066 0.76 +2.2 1,243,781 - Pf6N2E2_1066 0.76 +1.4 1,243,781 - Pf6N2E2_1066 0.76 +1.8 1,243,781 - Pf6N2E2_1066 0.76 +2.4 1,243,781 - Pf6N2E2_1066 0.76 +0.3 1,243,781 - Pf6N2E2_1066 0.76 +1.2 1,243,781 - Pf6N2E2_1066 0.76 -0.7 1,243,781 - Pf6N2E2_1066 0.76 +1.4 1,243,781 - Pf6N2E2_1066 0.76 +0.0 1,243,781 - Pf6N2E2_1066 0.76 -0.4 1,243,792 + Pf6N2E2_1066 0.77 +0.6 1,243,792 + Pf6N2E2_1066 0.77 +1.6 1,243,802 + Pf6N2E2_1066 0.78 -0.3 1,243,846 + Pf6N2E2_1066 0.83 -0.7 1,244,025 + +0.6 1,244,025 + +0.3 1,244,025 + +0.3 1,244,025 + +0.5 1,244,025 + -1.4 1,244,026 - -0.7 1,244,026 - -0.5 1,244,026 - -0.3 1,244,159 - -1.1 1,244,330 + Pf6N2E2_1067 0.19 -0.1 1,244,330 + Pf6N2E2_1067 0.19 +0.5 1,244,330 + Pf6N2E2_1067 0.19 -1.2 1,244,334 + Pf6N2E2_1067 0.20 -2.2 1,244,362 - Pf6N2E2_1067 0.21 -1.2 1,244,404 - Pf6N2E2_1067 0.24 -0.6 1,244,404 - Pf6N2E2_1067 0.24 -0.9 1,244,532 + Pf6N2E2_1067 0.32 -0.4 1,244,532 + Pf6N2E2_1067 0.32 +0.5 1,244,533 - Pf6N2E2_1067 0.32 +1.7 1,244,858 - Pf6N2E2_1067 0.52 +0.8 1,244,923 - Pf6N2E2_1067 0.56 -0.4 1,244,923 - Pf6N2E2_1067 0.56 -1.2 1,245,075 + Pf6N2E2_1067 0.65 -1.1 1,245,075 + Pf6N2E2_1067 0.65 +2.5 1,245,081 + Pf6N2E2_1067 0.66 -2.0 1,245,082 - Pf6N2E2_1067 0.66 +0.5 1,245,082 - Pf6N2E2_1067 0.66 +2.8 1,245,208 - Pf6N2E2_1067 0.73 -1.2 1,245,381 + Pf6N2E2_1067 0.84 -0.7 1,245,382 - Pf6N2E2_1067 0.84 +2.4 1,245,546 + +1.3 1,245,546 + -0.4 1,245,547 - -1.1 1,245,687 + +1.7 1,245,689 + -0.1 1,245,689 + +2.0 1,245,689 + +0.1 1,245,690 - +0.5 1,245,700 + -0.1 1,245,706 + +0.5 1,245,706 + -0.9 1,245,706 + +0.2 1,245,706 + +0.3 1,245,707 - -1.6 1,245,708 + -0.1 1,245,761 + +1.4 1,245,761 + +0.9 1,245,762 - +1.2 1,245,762 - -0.1 1,245,867 - +0.2 1,245,908 - -1.5 1,245,922 + +0.9 1,245,923 - -0.8 1,245,923 - -0.0 1,245,923 - -0.2 1,245,923 - -0.2 1,245,923 - +0.6 1,246,069 + Pf6N2E2_1068 0.25 -0.0 1,246,070 - Pf6N2E2_1068 0.25 -0.9 1,246,070 - Pf6N2E2_1068 0.25 -0.2 1,246,099 + Pf6N2E2_1068 0.29 +0.7 1,246,099 + Pf6N2E2_1068 0.29 -1.2 1,246,100 - Pf6N2E2_1068 0.29 -2.2 1,246,100 - Pf6N2E2_1068 0.29 +0.3 1,246,100 - Pf6N2E2_1068 0.29 -1.8 1,246,100 - Pf6N2E2_1068 0.29 -0.6 1,246,103 + Pf6N2E2_1068 0.29 +1.2 1,246,104 - Pf6N2E2_1068 0.29 +0.7 1,246,104 - Pf6N2E2_1068 0.29 -0.5 1,246,104 - Pf6N2E2_1068 0.29 +0.2 1,246,325 - Pf6N2E2_1068 0.53 -0.0 1,246,627 + Pf6N2E2_1068 0.86 -0.0
Or see this region's nucleotide sequence