Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06380 and MMP_RS06385 are separated by 5 nucleotides MMP_RS06385 and MMP_RS06390 are separated by 10 nucleotides MMP_RS06390 and MMP_RS06395 are separated by 52 nucleotides
MMP_RS06380: MMP_RS06380 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,225,667 to 1,226,809
_RS06380
MMP_RS06385: MMP_RS06385 - hypothetical protein, at 1,226,815 to 1,227,174
_RS06385
MMP_RS06390: MMP_RS06390 - quinolinate synthase NadA, at 1,227,185 to 1,228,102
_RS06390
MMP_RS06395: MMP_RS06395 - ThiF family adenylyltransferase, at 1,228,155 to 1,228,769
_RS06395
Position (kb)
1226
1227
1228 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1225.902 kb on - strand at 1226.409 kb on - strand at 1226.873 kb on + strand, within MMP_RS06385 at 1226.978 kb on - strand, within MMP_RS06385 at 1226.990 kb on + strand, within MMP_RS06385 at 1227.074 kb on - strand, within MMP_RS06385 at 1227.075 kb on + strand, within MMP_RS06385 at 1227.080 kb on - strand, within MMP_RS06385 at 1227.173 kb on + strand at 1227.710 kb on + strand at 1227.766 kb on - strand at 1227.771 kb on + strand at 1227.779 kb on - strand at 1227.821 kb on + strand at 1228.036 kb on + strand at 1228.094 kb on - strand at 1228.094 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 1,225,902 - -3.5 1,226,409 - -1.6 1,226,873 + MMP_RS06385 0.16 +0.1 1,226,978 - MMP_RS06385 0.45 +0.1 1,226,990 + MMP_RS06385 0.49 -0.0 1,227,074 - MMP_RS06385 0.72 +0.0 1,227,075 + MMP_RS06385 0.72 -0.4 1,227,080 - MMP_RS06385 0.74 -0.0 1,227,173 + +0.2 1,227,710 + -0.2 1,227,766 - -1.3 1,227,771 + +1.1 1,227,779 - -0.9 1,227,821 + -1.2 1,228,036 + -1.4 1,228,094 - -0.7 1,228,094 - -2.2
Or see this region's nucleotide sequence