Strain Fitness in Methanococcus maripaludis S2 around MMP_RS05035

Experiment: Formate McCas no stress control 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS05030 and MMP_RS05035 are separated by 49 nucleotidesMMP_RS05035 and MMP_RS05040 are separated by 77 nucleotidesMMP_RS05040 and MMP_RS05045 overlap by 14 nucleotides MMP_RS05030: MMP_RS05030 - adenylosuccinate lyase, at 958,096 to 959,445 _RS05030 MMP_RS05035: MMP_RS05035 - hypothetical protein, at 959,495 to 959,908 _RS05035 MMP_RS05040: MMP_RS05040 - permease, at 959,986 to 960,843 _RS05040 MMP_RS05045: MMP_RS05045 - 4Fe-4S binding protein, at 960,830 to 961,489 _RS05045 Position (kb) 959 960Strain fitness (log2 ratio) -3 -2 -1 0 1at 958.615 kb on + strandat 959.103 kb on + strandat 959.206 kb on + strandat 959.206 kb on + strandat 959.206 kb on + strandat 959.338 kb on - strandat 959.339 kb on + strandat 959.384 kb on + strandat 959.443 kb on - strandat 959.463 kb on + strandat 959.469 kb on - strandat 959.469 kb on - strandat 959.469 kb on - strandat 959.470 kb on + strandat 959.470 kb on + strandat 959.507 kb on + strandat 959.508 kb on - strandat 959.513 kb on - strandat 959.513 kb on - strandat 959.569 kb on + strand, within MMP_RS05035at 959.574 kb on + strand, within MMP_RS05035at 959.591 kb on + strand, within MMP_RS05035at 959.663 kb on + strand, within MMP_RS05035at 959.666 kb on - strand, within MMP_RS05035at 959.734 kb on - strand, within MMP_RS05035at 959.738 kb on - strand, within MMP_RS05035at 959.739 kb on + strand, within MMP_RS05035at 959.739 kb on + strand, within MMP_RS05035at 959.911 kb on - strandat 959.925 kb on + strandat 959.935 kb on - strandat 959.939 kb on + strandat 959.952 kb on - strandat 959.952 kb on - strandat 959.952 kb on - strandat 959.952 kb on - strandat 959.953 kb on + strandat 959.953 kb on + strandat 959.953 kb on + strandat 959.953 kb on + strandat 959.953 kb on + strandat 959.954 kb on - strandat 959.956 kb on - strandat 959.956 kb on - strandat 959.957 kb on + strandat 959.957 kb on + strandat 959.997 kb on - strandat 959.997 kb on - strandat 959.998 kb on + strandat 959.998 kb on + strandat 959.998 kb on + strandat 960.030 kb on - strandat 960.030 kb on - strandat 960.035 kb on + strandat 960.048 kb on + strandat 960.107 kb on + strand, within MMP_RS05040at 960.107 kb on + strand, within MMP_RS05040at 960.139 kb on + strand, within MMP_RS05040at 960.174 kb on - strand, within MMP_RS05040at 960.175 kb on + strand, within MMP_RS05040at 960.175 kb on + strand, within MMP_RS05040at 960.175 kb on + strand, within MMP_RS05040at 960.208 kb on - strand, within MMP_RS05040at 960.208 kb on - strand, within MMP_RS05040at 960.209 kb on + strand, within MMP_RS05040at 960.210 kb on - strand, within MMP_RS05040at 960.210 kb on - strand, within MMP_RS05040at 960.239 kb on - strand, within MMP_RS05040at 960.246 kb on - strand, within MMP_RS05040at 960.246 kb on - strand, within MMP_RS05040at 960.267 kb on + strand, within MMP_RS05040at 960.294 kb on + strand, within MMP_RS05040at 960.473 kb on + strand, within MMP_RS05040at 960.512 kb on - strand, within MMP_RS05040at 960.512 kb on - strand, within MMP_RS05040at 960.557 kb on + strand, within MMP_RS05040at 960.558 kb on - strand, within MMP_RS05040at 960.571 kb on + strand, within MMP_RS05040at 960.571 kb on + strand, within MMP_RS05040at 960.667 kb on + strand, within MMP_RS05040at 960.667 kb on + strand, within MMP_RS05040at 960.697 kb on + strand, within MMP_RS05040at 960.743 kb on - strand, within MMP_RS05040at 960.743 kb on - strand, within MMP_RS05040at 960.744 kb on + strand, within MMP_RS05040at 960.769 kb on - strandat 960.773 kb on - strandat 960.774 kb on + strandat 960.819 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas no stress control 1
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958,615 + -1.6
959,103 + -1.0
959,206 + -3.7
959,206 + -0.9
959,206 + -1.6
959,338 - -1.8
959,339 + -2.1
959,384 + -2.8
959,443 - -0.1
959,463 + +0.3
959,469 - +0.4
959,469 - -1.0
959,469 - +0.6
959,470 + -0.6
959,470 + -0.9
959,507 + -0.0
959,508 - -0.2
959,513 - +0.1
959,513 - +0.2
959,569 + MMP_RS05035 0.18 -0.0
959,574 + MMP_RS05035 0.19 -0.3
959,591 + MMP_RS05035 0.23 +0.4
959,663 + MMP_RS05035 0.41 -0.1
959,666 - MMP_RS05035 0.41 +0.1
959,734 - MMP_RS05035 0.58 +0.2
959,738 - MMP_RS05035 0.59 -0.0
959,739 + MMP_RS05035 0.59 +0.1
959,739 + MMP_RS05035 0.59 -0.4
959,911 - -0.1
959,925 + -0.3
959,935 - +0.3
959,939 + +0.1
959,952 - -0.3
959,952 - -0.3
959,952 - -0.7
959,952 - -0.2
959,953 + +0.6
959,953 + +0.2
959,953 + -0.1
959,953 + +0.0
959,953 + +0.0
959,954 - +0.6
959,956 - +0.4
959,956 - -0.4
959,957 + -0.0
959,957 + -0.0
959,997 - +0.1
959,997 - +0.0
959,998 + -0.3
959,998 + +0.2
959,998 + -0.1
960,030 - +0.1
960,030 - +0.2
960,035 + +0.3
960,048 + +0.1
960,107 + MMP_RS05040 0.14 +0.0
960,107 + MMP_RS05040 0.14 +0.4
960,139 + MMP_RS05040 0.18 +0.1
960,174 - MMP_RS05040 0.22 -1.0
960,175 + MMP_RS05040 0.22 +0.2
960,175 + MMP_RS05040 0.22 -0.1
960,175 + MMP_RS05040 0.22 -0.3
960,208 - MMP_RS05040 0.26 -0.2
960,208 - MMP_RS05040 0.26 +0.3
960,209 + MMP_RS05040 0.26 +0.5
960,210 - MMP_RS05040 0.26 -0.0
960,210 - MMP_RS05040 0.26 -0.0
960,239 - MMP_RS05040 0.29 -0.2
960,246 - MMP_RS05040 0.30 -0.3
960,246 - MMP_RS05040 0.30 +0.6
960,267 + MMP_RS05040 0.33 +0.3
960,294 + MMP_RS05040 0.36 +0.2
960,473 + MMP_RS05040 0.57 -0.1
960,512 - MMP_RS05040 0.61 -0.0
960,512 - MMP_RS05040 0.61 +0.4
960,557 + MMP_RS05040 0.67 -0.8
960,558 - MMP_RS05040 0.67 +0.3
960,571 + MMP_RS05040 0.68 -0.1
960,571 + MMP_RS05040 0.68 -0.1
960,667 + MMP_RS05040 0.79 +0.1
960,667 + MMP_RS05040 0.79 -0.3
960,697 + MMP_RS05040 0.83 +0.3
960,743 - MMP_RS05040 0.88 -0.1
960,743 - MMP_RS05040 0.88 -0.2
960,744 + MMP_RS05040 0.88 -0.2
960,769 - +0.3
960,773 - -0.0
960,774 + +0.4
960,819 - +0.8

Or see this region's nucleotide sequence