Experiment: selenate 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5914 and Pf6N2E2_5915 are separated by 3 nucleotides Pf6N2E2_5915 and Pf6N2E2_5916 overlap by 4 nucleotides
Pf6N2E2_5914 - Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly, at 6,732,086 to 6,733,306
_5914
Pf6N2E2_5915 - Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily, at 6,733,310 to 6,734,752
_5915
Pf6N2E2_5916 - Flp pilus assembly protein TadB, at 6,734,749 to 6,735,735
_5916
Position (kb)
6733
6734
6735 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6732.616 kb on + strand, within Pf6N2E2_5914 at 6732.616 kb on + strand, within Pf6N2E2_5914 at 6732.617 kb on - strand, within Pf6N2E2_5914 at 6732.662 kb on + strand, within Pf6N2E2_5914 at 6732.663 kb on - strand, within Pf6N2E2_5914 at 6732.663 kb on - strand, within Pf6N2E2_5914 at 6732.673 kb on - strand, within Pf6N2E2_5914 at 6732.857 kb on - strand, within Pf6N2E2_5914 at 6732.857 kb on - strand, within Pf6N2E2_5914 at 6732.863 kb on + strand, within Pf6N2E2_5914 at 6732.863 kb on + strand, within Pf6N2E2_5914 at 6732.863 kb on + strand, within Pf6N2E2_5914 at 6732.863 kb on + strand, within Pf6N2E2_5914 at 6732.864 kb on - strand, within Pf6N2E2_5914 at 6732.864 kb on - strand, within Pf6N2E2_5914 at 6732.941 kb on + strand, within Pf6N2E2_5914 at 6732.941 kb on + strand, within Pf6N2E2_5914 at 6732.942 kb on - strand, within Pf6N2E2_5914 at 6732.942 kb on - strand, within Pf6N2E2_5914 at 6733.274 kb on - strand at 6733.448 kb on + strand at 6733.448 kb on + strand at 6733.448 kb on + strand at 6733.503 kb on - strand, within Pf6N2E2_5915 at 6733.708 kb on + strand, within Pf6N2E2_5915 at 6733.709 kb on - strand, within Pf6N2E2_5915 at 6733.709 kb on - strand, within Pf6N2E2_5915 at 6733.709 kb on - strand, within Pf6N2E2_5915 at 6733.748 kb on + strand, within Pf6N2E2_5915 at 6734.196 kb on + strand, within Pf6N2E2_5915 at 6734.197 kb on - strand, within Pf6N2E2_5915 at 6734.197 kb on - strand, within Pf6N2E2_5915 at 6734.197 kb on - strand, within Pf6N2E2_5915 at 6734.267 kb on - strand, within Pf6N2E2_5915 at 6734.403 kb on + strand, within Pf6N2E2_5915 at 6734.403 kb on + strand, within Pf6N2E2_5915 at 6734.403 kb on + strand, within Pf6N2E2_5915 at 6734.403 kb on + strand, within Pf6N2E2_5915 at 6734.404 kb on - strand, within Pf6N2E2_5915 at 6734.404 kb on - strand, within Pf6N2E2_5915 at 6734.807 kb on + strand at 6734.808 kb on - strand at 6734.871 kb on + strand, within Pf6N2E2_5916 at 6734.872 kb on - strand, within Pf6N2E2_5916 at 6734.944 kb on + strand, within Pf6N2E2_5916 at 6734.944 kb on + strand, within Pf6N2E2_5916 at 6734.944 kb on + strand, within Pf6N2E2_5916 at 6734.945 kb on - strand, within Pf6N2E2_5916 at 6735.037 kb on + strand, within Pf6N2E2_5916 at 6735.098 kb on + strand, within Pf6N2E2_5916 at 6735.098 kb on + strand, within Pf6N2E2_5916 at 6735.099 kb on - strand, within Pf6N2E2_5916 at 6735.352 kb on + strand, within Pf6N2E2_5916 at 6735.352 kb on + strand, within Pf6N2E2_5916 at 6735.352 kb on + strand, within Pf6N2E2_5916 at 6735.353 kb on - strand, within Pf6N2E2_5916 at 6735.622 kb on + strand, within Pf6N2E2_5916 at 6735.622 kb on + strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916
Per-strain Table
Position Strand Gene LocusTag Fraction selenate 0.5 M remove 6,732,616 + Pf6N2E2_5914 0.43 +0.6 6,732,616 + Pf6N2E2_5914 0.43 -0.3 6,732,617 - Pf6N2E2_5914 0.43 +0.3 6,732,662 + Pf6N2E2_5914 0.47 +0.1 6,732,663 - Pf6N2E2_5914 0.47 -1.2 6,732,663 - Pf6N2E2_5914 0.47 +0.1 6,732,673 - Pf6N2E2_5914 0.48 +0.2 6,732,857 - Pf6N2E2_5914 0.63 +0.5 6,732,857 - Pf6N2E2_5914 0.63 -0.8 6,732,863 + Pf6N2E2_5914 0.64 +0.8 6,732,863 + Pf6N2E2_5914 0.64 -0.1 6,732,863 + Pf6N2E2_5914 0.64 -0.5 6,732,863 + Pf6N2E2_5914 0.64 -0.4 6,732,864 - Pf6N2E2_5914 0.64 +0.7 6,732,864 - Pf6N2E2_5914 0.64 -0.3 6,732,941 + Pf6N2E2_5914 0.70 +0.3 6,732,941 + Pf6N2E2_5914 0.70 +0.0 6,732,942 - Pf6N2E2_5914 0.70 -0.5 6,732,942 - Pf6N2E2_5914 0.70 -0.5 6,733,274 - +0.9 6,733,448 + -0.3 6,733,448 + -0.9 6,733,448 + +0.6 6,733,503 - Pf6N2E2_5915 0.13 +1.0 6,733,708 + Pf6N2E2_5915 0.28 +1.2 6,733,709 - Pf6N2E2_5915 0.28 -1.7 6,733,709 - Pf6N2E2_5915 0.28 -0.8 6,733,709 - Pf6N2E2_5915 0.28 -0.1 6,733,748 + Pf6N2E2_5915 0.30 +1.3 6,734,196 + Pf6N2E2_5915 0.61 +0.4 6,734,197 - Pf6N2E2_5915 0.61 +1.6 6,734,197 - Pf6N2E2_5915 0.61 +0.6 6,734,197 - Pf6N2E2_5915 0.61 +0.8 6,734,267 - Pf6N2E2_5915 0.66 +0.9 6,734,403 + Pf6N2E2_5915 0.76 -0.8 6,734,403 + Pf6N2E2_5915 0.76 +0.6 6,734,403 + Pf6N2E2_5915 0.76 +0.9 6,734,403 + Pf6N2E2_5915 0.76 +0.4 6,734,404 - Pf6N2E2_5915 0.76 +2.4 6,734,404 - Pf6N2E2_5915 0.76 +1.5 6,734,807 + -0.5 6,734,808 - -0.7 6,734,871 + Pf6N2E2_5916 0.12 -0.0 6,734,872 - Pf6N2E2_5916 0.12 +0.5 6,734,944 + Pf6N2E2_5916 0.20 +0.5 6,734,944 + Pf6N2E2_5916 0.20 -0.3 6,734,944 + Pf6N2E2_5916 0.20 -1.2 6,734,945 - Pf6N2E2_5916 0.20 -1.9 6,735,037 + Pf6N2E2_5916 0.29 -0.0 6,735,098 + Pf6N2E2_5916 0.35 +0.4 6,735,098 + Pf6N2E2_5916 0.35 -0.1 6,735,099 - Pf6N2E2_5916 0.35 -0.5 6,735,352 + Pf6N2E2_5916 0.61 -0.2 6,735,352 + Pf6N2E2_5916 0.61 -0.1 6,735,352 + Pf6N2E2_5916 0.61 +0.4 6,735,353 - Pf6N2E2_5916 0.61 +0.5 6,735,622 + Pf6N2E2_5916 0.88 +0.2 6,735,622 + Pf6N2E2_5916 0.88 +0.9 6,735,623 - Pf6N2E2_5916 0.89 +0.5 6,735,623 - Pf6N2E2_5916 0.89 -1.7 6,735,623 - Pf6N2E2_5916 0.89 -0.6 6,735,623 - Pf6N2E2_5916 0.89 -1.4 6,735,623 - Pf6N2E2_5916 0.89 -1.0
Or see this region's nucleotide sequence