Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_734

Experiment: Minimal media pH8

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_733 and Pf1N1B4_734 are separated by 129 nucleotidesPf1N1B4_734 and Pf1N1B4_735 overlap by 4 nucleotides Pf1N1B4_733 - Methionyl-tRNA synthetase (EC 6.1.1.10), at 835,415 to 837,466 _733 Pf1N1B4_734 - Electron transport complex protein RnfA, at 837,596 to 838,165 _734 Pf1N1B4_735 - Electron transport complex protein RnfB, at 838,162 to 839,169 _735 Pf1N1B4_736 - Electron transport complex protein RnfD, at 839,156 to 840,148 _736 Position (kb) 837 838 839Strain fitness (log2 ratio) -2 -1 0 1 2at 837.030 kb on + strand, within Pf1N1B4_733at 837.030 kb on + strand, within Pf1N1B4_733at 837.030 kb on + strand, within Pf1N1B4_733at 837.465 kb on - strandat 837.465 kb on - strandat 837.558 kb on + strandat 837.758 kb on + strand, within Pf1N1B4_734at 837.768 kb on - strand, within Pf1N1B4_734at 837.777 kb on + strand, within Pf1N1B4_734at 837.778 kb on - strand, within Pf1N1B4_734at 837.778 kb on - strand, within Pf1N1B4_734at 837.780 kb on + strand, within Pf1N1B4_734at 837.780 kb on + strand, within Pf1N1B4_734at 837.781 kb on - strand, within Pf1N1B4_734at 837.984 kb on + strand, within Pf1N1B4_734at 838.233 kb on + strandat 838.233 kb on + strandat 838.233 kb on + strandat 838.233 kb on + strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.241 kb on - strandat 838.241 kb on - strandat 838.384 kb on + strand, within Pf1N1B4_735at 838.385 kb on - strand, within Pf1N1B4_735at 838.385 kb on - strand, within Pf1N1B4_735at 839.155 kb on + strandat 839.156 kb on - strandat 839.156 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH8
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837,030 + Pf1N1B4_733 0.79 +0.2
837,030 + Pf1N1B4_733 0.79 -0.2
837,030 + Pf1N1B4_733 0.79 -0.6
837,465 - +1.2
837,465 - +0.6
837,558 + +0.5
837,758 + Pf1N1B4_734 0.28 -0.4
837,768 - Pf1N1B4_734 0.30 -0.4
837,777 + Pf1N1B4_734 0.32 -0.7
837,778 - Pf1N1B4_734 0.32 -0.6
837,778 - Pf1N1B4_734 0.32 -0.6
837,780 + Pf1N1B4_734 0.32 -1.0
837,780 + Pf1N1B4_734 0.32 -1.2
837,781 - Pf1N1B4_734 0.32 -0.2
837,984 + Pf1N1B4_734 0.68 -1.1
838,233 + +0.1
838,233 + -1.7
838,233 + +1.8
838,233 + +0.8
838,234 - -1.0
838,234 - -0.0
838,234 - +1.8
838,234 - -0.6
838,234 - +0.9
838,234 - +0.0
838,234 - +0.1
838,234 - -1.2
838,241 - -2.2
838,241 - -0.3
838,384 + Pf1N1B4_735 0.22 +0.2
838,385 - Pf1N1B4_735 0.22 +1.0
838,385 - Pf1N1B4_735 0.22 -2.3
839,155 + +0.4
839,156 - +0.8
839,156 - -0.2

Or see this region's nucleotide sequence