Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5699

Experiment: Minimal media pH8

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5698 and Pf1N1B4_5699 are separated by 139 nucleotidesPf1N1B4_5699 and Pf1N1B4_5700 are separated by 166 nucleotides Pf1N1B4_5698 - Putative monooxygenase, at 136,882 to 138,258 _5698 Pf1N1B4_5699 - ABC-type uncharacterized transport system, ATPase component, at 138,398 to 139,000 _5699 Pf1N1B4_5700 - Putative tRNA-m1A22 methylase, at 139,167 to 139,841 _5700 Position (kb) 138 139 140Strain fitness (log2 ratio) -2 -1 0 1at 137.431 kb on + strand, within Pf1N1B4_5698at 137.432 kb on - strandat 137.432 kb on - strand, within Pf1N1B4_5698at 137.489 kb on - strand, within Pf1N1B4_5698at 137.697 kb on - strand, within Pf1N1B4_5698at 137.804 kb on + strand, within Pf1N1B4_5698at 137.952 kb on - strand, within Pf1N1B4_5698at 137.952 kb on - strand, within Pf1N1B4_5698at 137.967 kb on + strand, within Pf1N1B4_5698at 137.968 kb on - strand, within Pf1N1B4_5698at 137.970 kb on - strand, within Pf1N1B4_5698at 137.970 kb on - strandat 137.972 kb on - strand, within Pf1N1B4_5698at 137.978 kb on - strand, within Pf1N1B4_5698at 138.085 kb on + strand, within Pf1N1B4_5698at 138.235 kb on - strandat 138.351 kb on - strandat 138.385 kb on + strandat 138.467 kb on + strand, within Pf1N1B4_5699at 138.488 kb on - strand, within Pf1N1B4_5699at 138.488 kb on - strand, within Pf1N1B4_5699at 138.488 kb on - strandat 138.717 kb on + strand, within Pf1N1B4_5699at 138.718 kb on - strand, within Pf1N1B4_5699at 139.058 kb on + strandat 139.065 kb on - strandat 139.096 kb on - strandat 139.096 kb on - strandat 139.144 kb on + strandat 139.346 kb on - strand, within Pf1N1B4_5700at 139.363 kb on + strand, within Pf1N1B4_5700at 139.363 kb on + strand, within Pf1N1B4_5700at 139.363 kb on + strand, within Pf1N1B4_5700at 139.364 kb on - strand, within Pf1N1B4_5700at 139.364 kb on - strand, within Pf1N1B4_5700at 139.364 kb on - strand, within Pf1N1B4_5700at 139.403 kb on - strand, within Pf1N1B4_5700at 139.406 kb on - strand, within Pf1N1B4_5700at 139.679 kb on + strand, within Pf1N1B4_5700at 139.679 kb on + strand, within Pf1N1B4_5700at 139.812 kb on + strandat 139.812 kb on + strandat 139.812 kb on + strandat 139.813 kb on - strandat 139.813 kb on - strandat 139.876 kb on + strandat 139.877 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH8
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137,431 + Pf1N1B4_5698 0.40 -0.5
137,432 - -0.1
137,432 - Pf1N1B4_5698 0.40 +0.9
137,489 - Pf1N1B4_5698 0.44 -0.2
137,697 - Pf1N1B4_5698 0.59 +0.3
137,804 + Pf1N1B4_5698 0.67 +0.0
137,952 - Pf1N1B4_5698 0.78 -1.7
137,952 - Pf1N1B4_5698 0.78 -1.7
137,967 + Pf1N1B4_5698 0.79 -0.3
137,968 - Pf1N1B4_5698 0.79 -0.5
137,970 - Pf1N1B4_5698 0.79 -2.2
137,970 - +0.1
137,972 - Pf1N1B4_5698 0.79 +0.1
137,978 - Pf1N1B4_5698 0.80 +0.1
138,085 + Pf1N1B4_5698 0.87 -1.0
138,235 - -0.2
138,351 - -1.5
138,385 + -0.6
138,467 + Pf1N1B4_5699 0.11 -0.6
138,488 - Pf1N1B4_5699 0.15 -0.5
138,488 - Pf1N1B4_5699 0.15 -1.5
138,488 - -1.0
138,717 + Pf1N1B4_5699 0.53 +0.7
138,718 - Pf1N1B4_5699 0.53 -2.3
139,058 + +0.3
139,065 - +0.2
139,096 - +1.6
139,096 - +1.3
139,144 + +0.5
139,346 - Pf1N1B4_5700 0.27 +0.1
139,363 + Pf1N1B4_5700 0.29 +0.3
139,363 + Pf1N1B4_5700 0.29 -0.4
139,363 + Pf1N1B4_5700 0.29 -0.1
139,364 - Pf1N1B4_5700 0.29 +0.6
139,364 - Pf1N1B4_5700 0.29 +0.2
139,364 - Pf1N1B4_5700 0.29 -1.2
139,403 - Pf1N1B4_5700 0.35 +1.3
139,406 - Pf1N1B4_5700 0.35 -0.1
139,679 + Pf1N1B4_5700 0.76 -0.7
139,679 + Pf1N1B4_5700 0.76 -0.1
139,812 + -0.7
139,812 + -0.3
139,812 + +0.6
139,813 - -0.6
139,813 - -0.2
139,876 + -0.6
139,877 - +0.8

Or see this region's nucleotide sequence