Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5150

Experiment: Minimal media pH8

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5149 and Pf1N1B4_5150 are separated by 2 nucleotides Pf1N1B4_5149 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 5,732,960 to 5,733,703 _5149 Pf1N1B4_5150 - FIG00933687: hypothetical protein, at 5,733,706 to 5,736,129 _5150 Position (kb) 5733 5734 5735 5736 5737Strain fitness (log2 ratio) -2 -1 0 1at 5732.860 kb on + strandat 5732.886 kb on - strandat 5733.172 kb on + strand, within Pf1N1B4_5149at 5733.172 kb on + strand, within Pf1N1B4_5149at 5733.173 kb on - strand, within Pf1N1B4_5149at 5733.173 kb on - strand, within Pf1N1B4_5149at 5733.173 kb on - strand, within Pf1N1B4_5149at 5733.173 kb on - strandat 5733.173 kb on - strand, within Pf1N1B4_5149at 5733.257 kb on + strand, within Pf1N1B4_5149at 5733.257 kb on + strand, within Pf1N1B4_5149at 5733.257 kb on + strand, within Pf1N1B4_5149at 5733.258 kb on - strand, within Pf1N1B4_5149at 5733.258 kb on - strand, within Pf1N1B4_5149at 5733.497 kb on + strand, within Pf1N1B4_5149at 5733.498 kb on - strand, within Pf1N1B4_5149at 5733.579 kb on + strand, within Pf1N1B4_5149at 5734.036 kb on + strand, within Pf1N1B4_5150at 5734.036 kb on + strand, within Pf1N1B4_5150at 5734.037 kb on - strand, within Pf1N1B4_5150at 5734.037 kb on - strand, within Pf1N1B4_5150at 5734.037 kb on - strand, within Pf1N1B4_5150at 5734.124 kb on - strand, within Pf1N1B4_5150at 5734.360 kb on + strandat 5734.694 kb on - strand, within Pf1N1B4_5150at 5734.930 kb on + strand, within Pf1N1B4_5150at 5734.930 kb on + strand, within Pf1N1B4_5150at 5734.930 kb on + strandat 5734.931 kb on - strand, within Pf1N1B4_5150at 5734.931 kb on - strand, within Pf1N1B4_5150at 5735.371 kb on + strand, within Pf1N1B4_5150at 5735.590 kb on + strandat 5735.675 kb on - strand, within Pf1N1B4_5150at 5735.827 kb on + strand, within Pf1N1B4_5150at 5735.828 kb on - strand, within Pf1N1B4_5150at 5736.101 kb on - strandat 5736.101 kb on - strandat 5736.182 kb on - strandat 5736.338 kb on + strandat 5736.339 kb on - strandat 5736.433 kb on + strandat 5736.481 kb on - strandat 5736.524 kb on - strandat 5736.546 kb on + strandat 5736.569 kb on - strandat 5736.921 kb on + strandat 5736.921 kb on + strandat 5736.999 kb on - strandat 5737.003 kb on - strandat 5737.009 kb on - strandat 5737.078 kb on + strandat 5737.127 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH8
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5,732,860 + -0.3
5,732,886 - -1.4
5,733,172 + Pf1N1B4_5149 0.28 -0.2
5,733,172 + Pf1N1B4_5149 0.28 -0.6
5,733,173 - Pf1N1B4_5149 0.29 -1.2
5,733,173 - Pf1N1B4_5149 0.29 +0.0
5,733,173 - Pf1N1B4_5149 0.29 -0.4
5,733,173 - +1.2
5,733,173 - Pf1N1B4_5149 0.29 -0.3
5,733,257 + Pf1N1B4_5149 0.40 +0.2
5,733,257 + Pf1N1B4_5149 0.40 +0.0
5,733,257 + Pf1N1B4_5149 0.40 -0.1
5,733,258 - Pf1N1B4_5149 0.40 +0.0
5,733,258 - Pf1N1B4_5149 0.40 -0.4
5,733,497 + Pf1N1B4_5149 0.72 +0.1
5,733,498 - Pf1N1B4_5149 0.72 +0.5
5,733,579 + Pf1N1B4_5149 0.83 -1.1
5,734,036 + Pf1N1B4_5150 0.14 +0.5
5,734,036 + Pf1N1B4_5150 0.14 -0.6
5,734,037 - Pf1N1B4_5150 0.14 -0.2
5,734,037 - Pf1N1B4_5150 0.14 +1.2
5,734,037 - Pf1N1B4_5150 0.14 +0.9
5,734,124 - Pf1N1B4_5150 0.17 -0.1
5,734,360 + +0.9
5,734,694 - Pf1N1B4_5150 0.41 +0.5
5,734,930 + Pf1N1B4_5150 0.50 +0.0
5,734,930 + Pf1N1B4_5150 0.50 +0.2
5,734,930 + +1.1
5,734,931 - Pf1N1B4_5150 0.51 +0.2
5,734,931 - Pf1N1B4_5150 0.51 +0.0
5,735,371 + Pf1N1B4_5150 0.69 +0.3
5,735,590 + +0.0
5,735,675 - Pf1N1B4_5150 0.81 +0.3
5,735,827 + Pf1N1B4_5150 0.88 +0.9
5,735,828 - Pf1N1B4_5150 0.88 +0.1
5,736,101 - +0.3
5,736,101 - +0.7
5,736,182 - -1.1
5,736,338 + +0.2
5,736,339 - +0.5
5,736,433 + -0.5
5,736,481 - +0.1
5,736,524 - -0.6
5,736,546 + +1.5
5,736,569 - -0.1
5,736,921 + +0.6
5,736,921 + -0.7
5,736,999 - -1.0
5,737,003 - -1.4
5,737,009 - -2.2
5,737,078 + -0.5
5,737,127 + -0.6

Or see this region's nucleotide sequence