Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_364

Experiment: Minimal media pH8

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_362 and Pf1N1B4_363 overlap by 8 nucleotidesPf1N1B4_363 and Pf1N1B4_364 are separated by 3 nucleotidesPf1N1B4_364 and Pf1N1B4_365 are separated by 286 nucleotidesPf1N1B4_365 and Pf1N1B4_366 are separated by 12 nucleotides Pf1N1B4_362 - Lipid A export ATP-binding/permease protein MsbA, at 430,596 to 432,413 _362 Pf1N1B4_363 - Thioredoxin, at 432,406 to 433,044 _363 Pf1N1B4_364 - Rhodanese domain protein UPF0176, at 433,048 to 433,989 _364 Pf1N1B4_365 - Cell division protein BolA, at 434,276 to 434,575 _365 Pf1N1B4_366 - FIG00954136: hypothetical protein, at 434,588 to 435,109 _366 Position (kb) 433 434Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 432.154 kb on + strandat 432.471 kb on - strand, within Pf1N1B4_363at 432.501 kb on + strand, within Pf1N1B4_363at 432.502 kb on - strand, within Pf1N1B4_363at 432.687 kb on - strand, within Pf1N1B4_363at 432.687 kb on - strand, within Pf1N1B4_363at 432.782 kb on + strand, within Pf1N1B4_363at 432.865 kb on - strand, within Pf1N1B4_363at 432.867 kb on - strand, within Pf1N1B4_363at 432.867 kb on - strand, within Pf1N1B4_363at 432.867 kb on - strand, within Pf1N1B4_363at 432.937 kb on + strand, within Pf1N1B4_363at 433.022 kb on + strandat 433.022 kb on + strandat 433.022 kb on + strandat 433.023 kb on - strandat 433.023 kb on - strandat 433.023 kb on - strandat 433.023 kb on - strandat 433.023 kb on - strandat 433.023 kb on - strandat 433.073 kb on + strandat 433.074 kb on - strandat 433.074 kb on - strandat 433.074 kb on - strandat 433.074 kb on - strandat 433.074 kb on - strandat 433.079 kb on + strandat 433.079 kb on + strandat 433.080 kb on - strandat 433.205 kb on + strand, within Pf1N1B4_364at 433.205 kb on + strand, within Pf1N1B4_364at 433.206 kb on - strand, within Pf1N1B4_364at 433.206 kb on - strand, within Pf1N1B4_364at 433.206 kb on - strand, within Pf1N1B4_364at 433.392 kb on - strand, within Pf1N1B4_364at 433.421 kb on + strand, within Pf1N1B4_364at 433.509 kb on - strand, within Pf1N1B4_364at 433.652 kb on + strand, within Pf1N1B4_364at 433.653 kb on - strand, within Pf1N1B4_364at 433.653 kb on - strand, within Pf1N1B4_364at 433.934 kb on + strandat 433.956 kb on + strandat 433.956 kb on + strandat 433.958 kb on + strandat 433.958 kb on + strandat 433.959 kb on - strandat 433.994 kb on - strandat 433.994 kb on - strandat 434.017 kb on + strandat 434.018 kb on - strandat 434.076 kb on - strandat 434.204 kb on - strandat 434.204 kb on - strandat 434.204 kb on - strandat 434.277 kb on + strandat 434.277 kb on + strandat 434.277 kb on + strandat 434.278 kb on - strandat 434.349 kb on + strand, within Pf1N1B4_365at 434.349 kb on + strand, within Pf1N1B4_365at 434.349 kb on + strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.350 kb on - strand, within Pf1N1B4_365at 434.403 kb on + strand, within Pf1N1B4_365at 434.404 kb on - strand, within Pf1N1B4_365at 434.404 kb on - strand, within Pf1N1B4_365at 434.590 kb on - strandat 434.620 kb on + strandat 434.761 kb on + strand, within Pf1N1B4_366at 434.763 kb on + strand, within Pf1N1B4_366at 434.763 kb on + strand, within Pf1N1B4_366at 434.763 kb on + strand, within Pf1N1B4_366at 434.763 kb on + strand, within Pf1N1B4_366at 434.764 kb on - strand, within Pf1N1B4_366at 434.764 kb on - strand, within Pf1N1B4_366at 434.811 kb on + strand, within Pf1N1B4_366at 434.811 kb on + strand, within Pf1N1B4_366at 434.811 kb on + strand, within Pf1N1B4_366at 434.811 kb on + strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.812 kb on - strand, within Pf1N1B4_366at 434.886 kb on + strand, within Pf1N1B4_366at 434.886 kb on + strandat 434.886 kb on + strand, within Pf1N1B4_366at 434.886 kb on + strand, within Pf1N1B4_366at 434.886 kb on + strand, within Pf1N1B4_366at 434.886 kb on + strandat 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.887 kb on - strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.967 kb on + strand, within Pf1N1B4_366at 434.968 kb on - strand, within Pf1N1B4_366at 434.968 kb on - strand, within Pf1N1B4_366at 434.968 kb on - strand, within Pf1N1B4_366at 434.968 kb on - strand, within Pf1N1B4_366

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH8
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432,154 + +1.6
432,471 - Pf1N1B4_363 0.10 -1.0
432,501 + Pf1N1B4_363 0.15 +0.1
432,502 - Pf1N1B4_363 0.15 +0.8
432,687 - Pf1N1B4_363 0.44 +0.6
432,687 - Pf1N1B4_363 0.44 -0.5
432,782 + Pf1N1B4_363 0.59 +0.2
432,865 - Pf1N1B4_363 0.72 +0.7
432,867 - Pf1N1B4_363 0.72 -0.1
432,867 - Pf1N1B4_363 0.72 +0.5
432,867 - Pf1N1B4_363 0.72 -0.5
432,937 + Pf1N1B4_363 0.83 +1.8
433,022 + -0.2
433,022 + -0.7
433,022 + +0.2
433,023 - +0.1
433,023 - -1.1
433,023 - -0.9
433,023 - -2.4
433,023 - -0.0
433,023 - +0.8
433,073 + -1.1
433,074 - -0.9
433,074 - -0.9
433,074 - -0.3
433,074 - +1.1
433,074 - -2.8
433,079 + -0.2
433,079 + +1.3
433,080 - -2.7
433,205 + Pf1N1B4_364 0.17 -1.2
433,205 + Pf1N1B4_364 0.17 -3.3
433,206 - Pf1N1B4_364 0.17 -1.4
433,206 - Pf1N1B4_364 0.17 -0.9
433,206 - Pf1N1B4_364 0.17 -0.8
433,392 - Pf1N1B4_364 0.37 -1.0
433,421 + Pf1N1B4_364 0.40 +0.6
433,509 - Pf1N1B4_364 0.49 +1.0
433,652 + Pf1N1B4_364 0.64 +0.5
433,653 - Pf1N1B4_364 0.64 -0.5
433,653 - Pf1N1B4_364 0.64 -0.7
433,934 + +0.2
433,956 + +0.3
433,956 + -0.1
433,958 + -2.1
433,958 + +0.1
433,959 - -0.1
433,994 - +0.4
433,994 - +0.6
434,017 + -1.2
434,018 - +0.5
434,076 - +0.3
434,204 - +0.4
434,204 - -1.4
434,204 - +1.0
434,277 + +0.6
434,277 + +0.4
434,277 + +1.6
434,278 - -0.1
434,349 + Pf1N1B4_365 0.24 +0.6
434,349 + Pf1N1B4_365 0.24 +0.0
434,349 + Pf1N1B4_365 0.24 -0.4
434,350 - Pf1N1B4_365 0.25 -0.5
434,350 - Pf1N1B4_365 0.25 -0.7
434,350 - Pf1N1B4_365 0.25 +0.1
434,350 - Pf1N1B4_365 0.25 -0.4
434,350 - Pf1N1B4_365 0.25 -1.2
434,350 - Pf1N1B4_365 0.25 -0.6
434,350 - Pf1N1B4_365 0.25 -0.5
434,350 - Pf1N1B4_365 0.25 +0.4
434,350 - Pf1N1B4_365 0.25 -0.4
434,350 - Pf1N1B4_365 0.25 +0.9
434,350 - Pf1N1B4_365 0.25 -0.9
434,350 - Pf1N1B4_365 0.25 -0.2
434,350 - Pf1N1B4_365 0.25 -0.9
434,403 + Pf1N1B4_365 0.42 -1.5
434,404 - Pf1N1B4_365 0.43 -1.4
434,404 - Pf1N1B4_365 0.43 -0.7
434,590 - -1.9
434,620 + +0.2
434,761 + Pf1N1B4_366 0.33 -3.1
434,763 + Pf1N1B4_366 0.34 +0.1
434,763 + Pf1N1B4_366 0.34 -1.0
434,763 + Pf1N1B4_366 0.34 -0.8
434,763 + Pf1N1B4_366 0.34 +1.7
434,764 - Pf1N1B4_366 0.34 +0.6
434,764 - Pf1N1B4_366 0.34 +0.2
434,811 + Pf1N1B4_366 0.43 +0.4
434,811 + Pf1N1B4_366 0.43 +0.6
434,811 + Pf1N1B4_366 0.43 +0.1
434,811 + Pf1N1B4_366 0.43 +0.8
434,812 - Pf1N1B4_366 0.43 +0.1
434,812 - Pf1N1B4_366 0.43 +1.5
434,812 - Pf1N1B4_366 0.43 +0.6
434,812 - Pf1N1B4_366 0.43 +0.6
434,812 - Pf1N1B4_366 0.43 -0.2
434,812 - Pf1N1B4_366 0.43 -0.1
434,886 + Pf1N1B4_366 0.57 -1.4
434,886 + -0.9
434,886 + Pf1N1B4_366 0.57 -0.8
434,886 + Pf1N1B4_366 0.57 +1.2
434,886 + Pf1N1B4_366 0.57 -0.4
434,886 + +0.2
434,887 - Pf1N1B4_366 0.57 +1.2
434,887 - Pf1N1B4_366 0.57 +0.3
434,887 - Pf1N1B4_366 0.57 -0.5
434,887 - Pf1N1B4_366 0.57 +0.3
434,887 - Pf1N1B4_366 0.57 -0.5
434,887 - Pf1N1B4_366 0.57 -0.2
434,887 - Pf1N1B4_366 0.57 +1.1
434,887 - Pf1N1B4_366 0.57 -1.0
434,967 + Pf1N1B4_366 0.73 -0.4
434,967 + Pf1N1B4_366 0.73 +0.1
434,967 + Pf1N1B4_366 0.73 +0.4
434,967 + Pf1N1B4_366 0.73 +0.3
434,967 + Pf1N1B4_366 0.73 -0.5
434,967 + Pf1N1B4_366 0.73 -0.2
434,967 + Pf1N1B4_366 0.73 +0.2
434,967 + Pf1N1B4_366 0.73 +0.8
434,967 + Pf1N1B4_366 0.73 -0.7
434,967 + Pf1N1B4_366 0.73 +1.2
434,967 + Pf1N1B4_366 0.73 -0.2
434,967 + Pf1N1B4_366 0.73 -0.2
434,968 - Pf1N1B4_366 0.73 -3.0
434,968 - Pf1N1B4_366 0.73 +1.0
434,968 - Pf1N1B4_366 0.73 +0.8
434,968 - Pf1N1B4_366 0.73 -0.1

Or see this region's nucleotide sequence