Experiment: Minimal media pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3347 and Pf1N1B4_3348 are separated by 81 nucleotides Pf1N1B4_3348 and Pf1N1B4_3349 are separated by 277 nucleotides Pf1N1B4_3349 and Pf1N1B4_3350 are separated by 136 nucleotides
Pf1N1B4_3347 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 3,734,867 to 3,735,799
_3347
Pf1N1B4_3348 - Nitroreductase family protein, at 3,735,881 to 3,736,474
_3348
Pf1N1B4_3349 - YcgN (Fragment), at 3,736,752 to 3,737,201
_3349
Pf1N1B4_3350 - FIG00954807: hypothetical protein, at 3,737,338 to 3,738,567
_3350
Position (kb)
3736
3737
3738 Strain fitness (log2 ratio)
-2
-1
0
1 at 3736.302 kb on + strand, within Pf1N1B4_3348 at 3736.329 kb on + strand, within Pf1N1B4_3348 at 3736.329 kb on + strand, within Pf1N1B4_3348 at 3736.447 kb on - strand at 3736.465 kb on + strand at 3736.489 kb on - strand at 3736.564 kb on + strand at 3736.564 kb on + strand at 3736.633 kb on + strand at 3736.633 kb on + strand at 3736.633 kb on + strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.646 kb on + strand at 3736.646 kb on + strand at 3736.648 kb on + strand at 3736.648 kb on + strand at 3736.648 kb on + strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.706 kb on + strand at 3736.709 kb on + strand at 3736.709 kb on + strand at 3736.709 kb on + strand at 3736.710 kb on - strand at 3736.710 kb on - strand at 3736.887 kb on + strand, within Pf1N1B4_3349 at 3736.887 kb on + strand, within Pf1N1B4_3349 at 3736.888 kb on - strand, within Pf1N1B4_3349 at 3736.888 kb on - strand, within Pf1N1B4_3349 at 3736.888 kb on - strand, within Pf1N1B4_3349 at 3736.888 kb on - strand, within Pf1N1B4_3349 at 3736.888 kb on - strand, within Pf1N1B4_3349 at 3736.890 kb on + strand at 3736.890 kb on + strand, within Pf1N1B4_3349 at 3736.890 kb on + strand at 3736.890 kb on + strand, within Pf1N1B4_3349 at 3736.891 kb on - strand, within Pf1N1B4_3349 at 3736.891 kb on - strand, within Pf1N1B4_3349 at 3736.891 kb on - strand, within Pf1N1B4_3349 at 3736.891 kb on - strand, within Pf1N1B4_3349 at 3736.891 kb on - strand, within Pf1N1B4_3349 at 3736.937 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand, within Pf1N1B4_3349 at 3737.117 kb on + strand at 3737.118 kb on - strand, within Pf1N1B4_3349 at 3737.135 kb on + strand, within Pf1N1B4_3349 at 3737.135 kb on + strand, within Pf1N1B4_3349 at 3737.135 kb on + strand, within Pf1N1B4_3349 at 3737.136 kb on - strand, within Pf1N1B4_3349 at 3737.136 kb on - strand, within Pf1N1B4_3349 at 3737.136 kb on - strand, within Pf1N1B4_3349 at 3737.136 kb on - strand, within Pf1N1B4_3349 at 3737.199 kb on + strand at 3737.247 kb on + strand at 3737.248 kb on - strand at 3737.291 kb on + strand at 3737.327 kb on + strand at 3737.327 kb on + strand at 3737.328 kb on - strand at 3737.328 kb on - strand at 3737.337 kb on + strand at 3737.425 kb on + strand at 3737.783 kb on + strand, within Pf1N1B4_3350 at 3738.113 kb on - strand, within Pf1N1B4_3350
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH8 remove 3,736,302 + Pf1N1B4_3348 0.71 -1.1 3,736,329 + Pf1N1B4_3348 0.75 -0.1 3,736,329 + Pf1N1B4_3348 0.75 -0.3 3,736,447 - +0.8 3,736,465 + +0.2 3,736,489 - +1.7 3,736,564 + -0.3 3,736,564 + +1.5 3,736,633 + -1.1 3,736,633 + +1.1 3,736,633 + +0.5 3,736,634 - -1.4 3,736,634 - -0.5 3,736,634 - +0.7 3,736,634 - +0.3 3,736,646 + +0.6 3,736,646 + +0.3 3,736,648 + +1.3 3,736,648 + +0.6 3,736,648 + -0.1 3,736,649 - +1.0 3,736,649 - +0.2 3,736,649 - +0.5 3,736,649 - -0.3 3,736,649 - +0.3 3,736,706 + +0.6 3,736,709 + -0.6 3,736,709 + -0.5 3,736,709 + -0.6 3,736,710 - +0.4 3,736,710 - +1.2 3,736,887 + Pf1N1B4_3349 0.30 -1.1 3,736,887 + Pf1N1B4_3349 0.30 -1.8 3,736,888 - Pf1N1B4_3349 0.30 -2.2 3,736,888 - Pf1N1B4_3349 0.30 +0.7 3,736,888 - Pf1N1B4_3349 0.30 -1.0 3,736,888 - Pf1N1B4_3349 0.30 -2.1 3,736,888 - Pf1N1B4_3349 0.30 -0.2 3,736,890 + +0.7 3,736,890 + Pf1N1B4_3349 0.31 +0.3 3,736,890 + -0.6 3,736,890 + Pf1N1B4_3349 0.31 -1.4 3,736,891 - Pf1N1B4_3349 0.31 -1.0 3,736,891 - Pf1N1B4_3349 0.31 -2.3 3,736,891 - Pf1N1B4_3349 0.31 -1.0 3,736,891 - Pf1N1B4_3349 0.31 -0.4 3,736,891 - Pf1N1B4_3349 0.31 +0.0 3,736,937 + Pf1N1B4_3349 0.41 -0.0 3,737,117 + Pf1N1B4_3349 0.81 -1.0 3,737,117 + Pf1N1B4_3349 0.81 +0.5 3,737,117 + Pf1N1B4_3349 0.81 -1.0 3,737,117 + Pf1N1B4_3349 0.81 -0.6 3,737,117 + Pf1N1B4_3349 0.81 -0.4 3,737,117 + Pf1N1B4_3349 0.81 -0.4 3,737,117 + Pf1N1B4_3349 0.81 -0.6 3,737,117 + Pf1N1B4_3349 0.81 +0.7 3,737,117 + -1.3 3,737,118 - Pf1N1B4_3349 0.81 +0.1 3,737,135 + Pf1N1B4_3349 0.85 -0.4 3,737,135 + Pf1N1B4_3349 0.85 -0.3 3,737,135 + Pf1N1B4_3349 0.85 -0.9 3,737,136 - Pf1N1B4_3349 0.85 -1.5 3,737,136 - Pf1N1B4_3349 0.85 -1.3 3,737,136 - Pf1N1B4_3349 0.85 -0.1 3,737,136 - Pf1N1B4_3349 0.85 -0.8 3,737,199 + -0.7 3,737,247 + +1.4 3,737,248 - -0.7 3,737,291 + -1.1 3,737,327 + +0.0 3,737,327 + +0.8 3,737,328 - +0.7 3,737,328 - -0.6 3,737,337 + +0.6 3,737,425 + -1.2 3,737,783 + Pf1N1B4_3350 0.36 +0.2 3,738,113 - Pf1N1B4_3350 0.63 +1.1
Or see this region's nucleotide sequence