Experiment: Minimal media pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2880 and Pf1N1B4_2881 are separated by 146 nucleotides Pf1N1B4_2881 and Pf1N1B4_2882 are separated by 63 nucleotides
Pf1N1B4_2880 - Ketol-acid reductoisomerase (EC 1.1.1.86), at 3,226,732 to 3,227,748
_2880
Pf1N1B4_2881 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 3,227,895 to 3,228,752
_2881
Pf1N1B4_2882 - Putative sulfite oxidase subunit YedY, at 3,228,816 to 3,229,829
_2882
Position (kb)
3227
3228
3229 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3226.996 kb on + strand, within Pf1N1B4_2880 at 3226.996 kb on + strand, within Pf1N1B4_2880 at 3226.996 kb on + strand, within Pf1N1B4_2880 at 3226.996 kb on + strand, within Pf1N1B4_2880 at 3226.997 kb on - strand, within Pf1N1B4_2880 at 3226.997 kb on - strand, within Pf1N1B4_2880 at 3226.997 kb on - strand, within Pf1N1B4_2880 at 3227.068 kb on + strand, within Pf1N1B4_2880 at 3227.068 kb on + strand, within Pf1N1B4_2880 at 3227.068 kb on + strand, within Pf1N1B4_2880 at 3227.069 kb on - strand at 3227.069 kb on - strand at 3227.185 kb on - strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand, within Pf1N1B4_2880 at 3227.188 kb on + strand at 3227.189 kb on - strand, within Pf1N1B4_2880 at 3227.189 kb on - strand, within Pf1N1B4_2880 at 3227.230 kb on + strand, within Pf1N1B4_2880 at 3227.230 kb on + strand, within Pf1N1B4_2880 at 3227.231 kb on - strand, within Pf1N1B4_2880 at 3227.348 kb on + strand, within Pf1N1B4_2880 at 3227.401 kb on + strand, within Pf1N1B4_2880 at 3227.401 kb on + strand, within Pf1N1B4_2880 at 3227.401 kb on + strand, within Pf1N1B4_2880 at 3227.401 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.446 kb on + strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.447 kb on - strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.473 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.506 kb on + strand, within Pf1N1B4_2880 at 3227.507 kb on - strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.571 kb on + strand, within Pf1N1B4_2880 at 3227.572 kb on - strand, within Pf1N1B4_2880 at 3227.572 kb on - strand, within Pf1N1B4_2880 at 3227.572 kb on - strand, within Pf1N1B4_2880 at 3227.587 kb on + strand, within Pf1N1B4_2880 at 3227.649 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.746 kb on + strand at 3227.777 kb on + strand at 3227.777 kb on + strand at 3227.777 kb on + strand at 3227.814 kb on + strand at 3227.814 kb on + strand at 3227.816 kb on + strand at 3228.898 kb on + strand at 3228.898 kb on + strand at 3228.901 kb on + strand at 3228.901 kb on + strand at 3228.901 kb on + strand at 3228.901 kb on + strand at 3229.104 kb on + strand, within Pf1N1B4_2882 at 3229.104 kb on + strand, within Pf1N1B4_2882 at 3229.105 kb on - strand, within Pf1N1B4_2882 at 3229.253 kb on + strand at 3229.253 kb on + strand, within Pf1N1B4_2882 at 3229.257 kb on + strand, within Pf1N1B4_2882 at 3229.257 kb on + strand, within Pf1N1B4_2882 at 3229.395 kb on - strand, within Pf1N1B4_2882 at 3229.437 kb on + strand, within Pf1N1B4_2882 at 3229.437 kb on + strand, within Pf1N1B4_2882 at 3229.438 kb on - strand, within Pf1N1B4_2882 at 3229.441 kb on + strand, within Pf1N1B4_2882 at 3229.441 kb on + strand, within Pf1N1B4_2882 at 3229.441 kb on + strand, within Pf1N1B4_2882 at 3229.441 kb on + strand, within Pf1N1B4_2882 at 3229.442 kb on - strand at 3229.442 kb on - strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.500 kb on + strand, within Pf1N1B4_2882 at 3229.501 kb on - strand, within Pf1N1B4_2882 at 3229.501 kb on - strand, within Pf1N1B4_2882 at 3229.560 kb on + strand, within Pf1N1B4_2882 at 3229.560 kb on + strand, within Pf1N1B4_2882 at 3229.622 kb on + strand, within Pf1N1B4_2882 at 3229.622 kb on + strand, within Pf1N1B4_2882 at 3229.623 kb on - strand, within Pf1N1B4_2882 at 3229.660 kb on - strand, within Pf1N1B4_2882
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH8 remove 3,226,996 + Pf1N1B4_2880 0.26 -3.0 3,226,996 + Pf1N1B4_2880 0.26 -2.3 3,226,996 + Pf1N1B4_2880 0.26 -4.4 3,226,996 + Pf1N1B4_2880 0.26 -3.5 3,226,997 - Pf1N1B4_2880 0.26 -0.5 3,226,997 - Pf1N1B4_2880 0.26 -1.8 3,226,997 - Pf1N1B4_2880 0.26 -2.7 3,227,068 + Pf1N1B4_2880 0.33 -1.2 3,227,068 + Pf1N1B4_2880 0.33 -4.4 3,227,068 + Pf1N1B4_2880 0.33 -4.4 3,227,069 - -0.7 3,227,069 - -1.1 3,227,185 - Pf1N1B4_2880 0.45 -3.3 3,227,188 + Pf1N1B4_2880 0.45 -1.2 3,227,188 + Pf1N1B4_2880 0.45 -2.7 3,227,188 + Pf1N1B4_2880 0.45 -3.4 3,227,188 + Pf1N1B4_2880 0.45 -3.1 3,227,188 + Pf1N1B4_2880 0.45 -2.1 3,227,188 + Pf1N1B4_2880 0.45 -1.9 3,227,188 + -1.1 3,227,189 - Pf1N1B4_2880 0.45 -2.7 3,227,189 - Pf1N1B4_2880 0.45 -2.2 3,227,230 + Pf1N1B4_2880 0.49 -3.6 3,227,230 + Pf1N1B4_2880 0.49 -3.5 3,227,231 - Pf1N1B4_2880 0.49 -2.5 3,227,348 + Pf1N1B4_2880 0.61 -2.3 3,227,401 + Pf1N1B4_2880 0.66 -2.7 3,227,401 + Pf1N1B4_2880 0.66 -2.8 3,227,401 + Pf1N1B4_2880 0.66 -3.9 3,227,401 + Pf1N1B4_2880 0.66 -2.7 3,227,446 + Pf1N1B4_2880 0.70 -2.4 3,227,446 + Pf1N1B4_2880 0.70 -4.8 3,227,446 + Pf1N1B4_2880 0.70 -3.7 3,227,446 + Pf1N1B4_2880 0.70 -2.4 3,227,446 + Pf1N1B4_2880 0.70 -3.1 3,227,446 + Pf1N1B4_2880 0.70 -3.9 3,227,446 + Pf1N1B4_2880 0.70 -2.9 3,227,446 + Pf1N1B4_2880 0.70 -2.0 3,227,446 + Pf1N1B4_2880 0.70 -4.0 3,227,446 + Pf1N1B4_2880 0.70 -2.9 3,227,446 + Pf1N1B4_2880 0.70 -1.1 3,227,446 + Pf1N1B4_2880 0.70 -2.5 3,227,446 + Pf1N1B4_2880 0.70 -3.2 3,227,446 + Pf1N1B4_2880 0.70 -3.1 3,227,446 + Pf1N1B4_2880 0.70 -2.3 3,227,446 + Pf1N1B4_2880 0.70 -4.2 3,227,446 + Pf1N1B4_2880 0.70 -2.7 3,227,446 + Pf1N1B4_2880 0.70 -1.4 3,227,446 + Pf1N1B4_2880 0.70 -2.2 3,227,447 - Pf1N1B4_2880 0.70 -2.9 3,227,447 - Pf1N1B4_2880 0.70 -2.7 3,227,447 - Pf1N1B4_2880 0.70 -1.6 3,227,447 - Pf1N1B4_2880 0.70 -2.1 3,227,447 - Pf1N1B4_2880 0.70 -3.9 3,227,447 - Pf1N1B4_2880 0.70 -2.3 3,227,473 + Pf1N1B4_2880 0.73 -1.5 3,227,473 + Pf1N1B4_2880 0.73 -3.5 3,227,473 + Pf1N1B4_2880 0.73 -1.1 3,227,473 + Pf1N1B4_2880 0.73 -3.2 3,227,473 + Pf1N1B4_2880 0.73 -2.4 3,227,473 + Pf1N1B4_2880 0.73 -1.0 3,227,506 + Pf1N1B4_2880 0.76 -4.3 3,227,506 + Pf1N1B4_2880 0.76 -1.7 3,227,506 + Pf1N1B4_2880 0.76 -3.8 3,227,506 + Pf1N1B4_2880 0.76 -2.3 3,227,506 + Pf1N1B4_2880 0.76 -2.0 3,227,506 + Pf1N1B4_2880 0.76 -2.5 3,227,507 - Pf1N1B4_2880 0.76 -1.7 3,227,571 + Pf1N1B4_2880 0.82 -2.5 3,227,571 + Pf1N1B4_2880 0.82 -3.1 3,227,571 + Pf1N1B4_2880 0.82 -1.8 3,227,571 + Pf1N1B4_2880 0.82 -4.3 3,227,571 + Pf1N1B4_2880 0.82 -4.7 3,227,571 + Pf1N1B4_2880 0.82 -3.5 3,227,571 + Pf1N1B4_2880 0.82 -3.0 3,227,571 + Pf1N1B4_2880 0.82 -2.1 3,227,571 + Pf1N1B4_2880 0.82 -1.8 3,227,571 + Pf1N1B4_2880 0.82 -3.0 3,227,572 - Pf1N1B4_2880 0.83 -2.2 3,227,572 - Pf1N1B4_2880 0.83 -3.0 3,227,572 - Pf1N1B4_2880 0.83 -3.5 3,227,587 + Pf1N1B4_2880 0.84 -1.1 3,227,649 + -1.7 3,227,746 + -0.1 3,227,746 + -2.4 3,227,746 + -1.0 3,227,746 + -1.2 3,227,746 + -0.6 3,227,746 + -2.3 3,227,746 + -0.5 3,227,746 + -1.0 3,227,746 + -3.1 3,227,746 + -1.5 3,227,746 + -1.5 3,227,746 + -0.4 3,227,777 + -2.2 3,227,777 + -2.9 3,227,777 + -0.0 3,227,814 + +0.0 3,227,814 + +0.7 3,227,816 + +0.3 3,228,898 + +1.9 3,228,898 + -1.3 3,228,901 + +0.4 3,228,901 + -0.8 3,228,901 + +0.4 3,228,901 + +1.9 3,229,104 + Pf1N1B4_2882 0.28 +0.6 3,229,104 + Pf1N1B4_2882 0.28 -1.3 3,229,105 - Pf1N1B4_2882 0.29 +0.5 3,229,253 + -0.7 3,229,253 + Pf1N1B4_2882 0.43 -1.0 3,229,257 + Pf1N1B4_2882 0.43 -0.0 3,229,257 + Pf1N1B4_2882 0.43 +0.0 3,229,395 - Pf1N1B4_2882 0.57 +1.4 3,229,437 + Pf1N1B4_2882 0.61 -1.4 3,229,437 + Pf1N1B4_2882 0.61 -0.0 3,229,438 - Pf1N1B4_2882 0.61 -0.1 3,229,441 + Pf1N1B4_2882 0.62 +0.0 3,229,441 + Pf1N1B4_2882 0.62 -0.6 3,229,441 + Pf1N1B4_2882 0.62 +0.8 3,229,441 + Pf1N1B4_2882 0.62 -1.1 3,229,442 - +1.9 3,229,442 - Pf1N1B4_2882 0.62 +0.3 3,229,500 + Pf1N1B4_2882 0.67 -1.0 3,229,500 + Pf1N1B4_2882 0.67 -0.4 3,229,500 + Pf1N1B4_2882 0.67 -2.1 3,229,500 + Pf1N1B4_2882 0.67 -0.5 3,229,500 + Pf1N1B4_2882 0.67 -0.7 3,229,500 + Pf1N1B4_2882 0.67 -0.6 3,229,500 + Pf1N1B4_2882 0.67 -0.4 3,229,500 + Pf1N1B4_2882 0.67 -1.3 3,229,501 - Pf1N1B4_2882 0.68 -0.6 3,229,501 - Pf1N1B4_2882 0.68 -1.6 3,229,560 + Pf1N1B4_2882 0.73 -0.1 3,229,560 + Pf1N1B4_2882 0.73 +0.0 3,229,622 + Pf1N1B4_2882 0.79 -0.1 3,229,622 + Pf1N1B4_2882 0.79 +0.4 3,229,623 - Pf1N1B4_2882 0.80 +0.3 3,229,660 - Pf1N1B4_2882 0.83 -0.4
Or see this region's nucleotide sequence