Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1052

Experiment: Minimal media pH8

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1050 and Pf1N1B4_1051 are separated by 226 nucleotidesPf1N1B4_1051 and Pf1N1B4_1052 are separated by 179 nucleotidesPf1N1B4_1052 and Pf1N1B4_1053 are separated by 40 nucleotidesPf1N1B4_1053 and Pf1N1B4_1054 are separated by 15 nucleotides Pf1N1B4_1050 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 1,186,216 to 1,187,319 _1050 Pf1N1B4_1051 - Glutathione peroxidase family protein, at 1,187,546 to 1,187,974 _1051 Pf1N1B4_1052 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 1,188,154 to 1,188,639 _1052 Pf1N1B4_1053 - hypothetical protein, at 1,188,680 to 1,188,991 _1053 Pf1N1B4_1054 - Phosphate acetyltransferase (EC 2.3.1.8), at 1,189,007 to 1,189,840 _1054 Position (kb) 1188 1189Strain fitness (log2 ratio) -2 -1 0 1 2at 1187.189 kb on + strand, within Pf1N1B4_1050at 1187.192 kb on + strand, within Pf1N1B4_1050at 1187.192 kb on + strand, within Pf1N1B4_1050at 1187.338 kb on + strandat 1187.338 kb on + strandat 1187.338 kb on + strandat 1187.339 kb on - strandat 1187.345 kb on + strandat 1187.346 kb on - strandat 1187.359 kb on + strandat 1187.389 kb on - strandat 1187.389 kb on - strandat 1187.396 kb on + strandat 1187.396 kb on + strandat 1187.396 kb on + strandat 1187.396 kb on + strandat 1187.396 kb on + strandat 1187.396 kb on + strandat 1187.397 kb on - strandat 1187.397 kb on - strandat 1187.397 kb on - strandat 1187.431 kb on - strandat 1187.475 kb on + strandat 1187.475 kb on + strandat 1187.476 kb on - strandat 1187.501 kb on + strandat 1187.599 kb on + strand, within Pf1N1B4_1051at 1187.709 kb on + strand, within Pf1N1B4_1051at 1187.709 kb on + strand, within Pf1N1B4_1051at 1187.709 kb on + strand, within Pf1N1B4_1051at 1187.710 kb on - strand, within Pf1N1B4_1051at 1187.772 kb on + strand, within Pf1N1B4_1051at 1187.772 kb on + strand, within Pf1N1B4_1051at 1187.773 kb on - strand, within Pf1N1B4_1051at 1187.773 kb on - strand, within Pf1N1B4_1051at 1187.773 kb on - strand, within Pf1N1B4_1051at 1187.874 kb on + strand, within Pf1N1B4_1051at 1187.874 kb on + strand, within Pf1N1B4_1051at 1187.874 kb on + strand, within Pf1N1B4_1051at 1187.874 kb on + strand, within Pf1N1B4_1051at 1187.874 kb on + strandat 1187.875 kb on - strand, within Pf1N1B4_1051at 1187.875 kb on - strand, within Pf1N1B4_1051at 1187.875 kb on - strand, within Pf1N1B4_1051at 1187.875 kb on - strand, within Pf1N1B4_1051at 1187.875 kb on - strand, within Pf1N1B4_1051at 1187.875 kb on - strand, within Pf1N1B4_1051at 1187.875 kb on - strand, within Pf1N1B4_1051at 1187.904 kb on + strand, within Pf1N1B4_1051at 1187.905 kb on - strand, within Pf1N1B4_1051at 1187.978 kb on + strandat 1187.979 kb on - strandat 1187.979 kb on - strandat 1187.979 kb on - strandat 1187.979 kb on - strandat 1187.979 kb on - strandat 1188.100 kb on - strandat 1188.428 kb on + strand, within Pf1N1B4_1052at 1188.429 kb on - strand, within Pf1N1B4_1052at 1188.429 kb on - strand, within Pf1N1B4_1052at 1188.454 kb on + strand, within Pf1N1B4_1052at 1188.455 kb on - strand, within Pf1N1B4_1052at 1188.455 kb on - strand, within Pf1N1B4_1052at 1188.522 kb on + strand, within Pf1N1B4_1052at 1188.539 kb on + strand, within Pf1N1B4_1052at 1188.601 kb on - strandat 1188.601 kb on - strandat 1188.602 kb on + strandat 1188.603 kb on - strandat 1188.603 kb on - strandat 1188.621 kb on + strandat 1188.871 kb on + strand, within Pf1N1B4_1053at 1188.871 kb on + strand, within Pf1N1B4_1053at 1188.968 kb on + strandat 1189.283 kb on + strand, within Pf1N1B4_1054at 1189.283 kb on + strand, within Pf1N1B4_1054at 1189.352 kb on + strand, within Pf1N1B4_1054at 1189.352 kb on + strand, within Pf1N1B4_1054

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH8
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1,187,189 + Pf1N1B4_1050 0.88 -1.5
1,187,192 + Pf1N1B4_1050 0.88 +0.2
1,187,192 + Pf1N1B4_1050 0.88 -1.0
1,187,338 + +0.1
1,187,338 + +0.4
1,187,338 + +0.1
1,187,339 - -0.9
1,187,345 + +0.2
1,187,346 - -2.2
1,187,359 + -0.8
1,187,389 - -0.8
1,187,389 - +0.8
1,187,396 + -0.4
1,187,396 + -0.9
1,187,396 + -0.1
1,187,396 + -0.3
1,187,396 + +0.8
1,187,396 + +1.2
1,187,397 - +0.4
1,187,397 - +0.2
1,187,397 - -0.7
1,187,431 - +0.2
1,187,475 + +0.4
1,187,475 + +2.5
1,187,476 - +0.6
1,187,501 + -0.1
1,187,599 + Pf1N1B4_1051 0.12 -0.7
1,187,709 + Pf1N1B4_1051 0.38 -1.5
1,187,709 + Pf1N1B4_1051 0.38 -1.2
1,187,709 + Pf1N1B4_1051 0.38 -0.1
1,187,710 - Pf1N1B4_1051 0.38 -1.7
1,187,772 + Pf1N1B4_1051 0.53 -0.4
1,187,772 + Pf1N1B4_1051 0.53 +0.3
1,187,773 - Pf1N1B4_1051 0.53 +1.1
1,187,773 - Pf1N1B4_1051 0.53 +1.1
1,187,773 - Pf1N1B4_1051 0.53 +0.2
1,187,874 + Pf1N1B4_1051 0.76 +0.1
1,187,874 + Pf1N1B4_1051 0.76 +0.8
1,187,874 + Pf1N1B4_1051 0.76 +0.1
1,187,874 + Pf1N1B4_1051 0.76 -0.8
1,187,874 + -0.1
1,187,875 - Pf1N1B4_1051 0.77 +1.3
1,187,875 - Pf1N1B4_1051 0.77 -0.5
1,187,875 - Pf1N1B4_1051 0.77 +0.7
1,187,875 - Pf1N1B4_1051 0.77 +0.6
1,187,875 - Pf1N1B4_1051 0.77 +0.1
1,187,875 - Pf1N1B4_1051 0.77 -2.7
1,187,875 - Pf1N1B4_1051 0.77 +0.7
1,187,904 + Pf1N1B4_1051 0.83 +0.0
1,187,905 - Pf1N1B4_1051 0.84 -2.2
1,187,978 + +0.8
1,187,979 - +1.1
1,187,979 - +0.8
1,187,979 - +1.0
1,187,979 - -1.0
1,187,979 - -0.0
1,188,100 - +0.2
1,188,428 + Pf1N1B4_1052 0.56 +0.1
1,188,429 - Pf1N1B4_1052 0.57 -0.3
1,188,429 - Pf1N1B4_1052 0.57 +0.2
1,188,454 + Pf1N1B4_1052 0.62 -0.9
1,188,455 - Pf1N1B4_1052 0.62 +0.0
1,188,455 - Pf1N1B4_1052 0.62 -0.5
1,188,522 + Pf1N1B4_1052 0.76 -1.1
1,188,539 + Pf1N1B4_1052 0.79 -2.2
1,188,601 - +0.1
1,188,601 - +1.3
1,188,602 + -1.2
1,188,603 - -0.3
1,188,603 - +1.1
1,188,621 + -0.1
1,188,871 + Pf1N1B4_1053 0.61 +0.5
1,188,871 + Pf1N1B4_1053 0.61 +1.8
1,188,968 + -0.7
1,189,283 + Pf1N1B4_1054 0.33 +0.0
1,189,283 + Pf1N1B4_1054 0.33 -0.3
1,189,352 + Pf1N1B4_1054 0.41 +1.7
1,189,352 + Pf1N1B4_1054 0.41 -0.4

Or see this region's nucleotide sequence