Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01405 and MMP_RS01410 are separated by 178 nucleotides MMP_RS01410 and MMP_RS01415 overlap by 4 nucleotides MMP_RS01415 and MMP_RS08935 are separated by 331 nucleotides MMP_RS08935 and MMP_RS01420 are separated by 241 nucleotides
MMP_RS01405: MMP_RS01405 - 50S ribosomal protein L1, at 261,024 to 261,665
_RS01405
MMP_RS01410: MMP_RS01410 - DNA-directed RNA polymerase subunit L, at 261,844 to 262,134
_RS01410
MMP_RS01415: MMP_RS01415 - DUF2067 domain-containing protein, at 262,131 to 262,748
_RS01415
MMP_RS08935: MMP_RS08935 - hypothetical protein, at 263,080 to 263,229
_RS08935
MMP_RS01420: MMP_RS01420 - hypothetical protein, at 263,471 to 263,668
_RS01420
Position (kb)
262
263 Strain fitness (log2 ratio)
-2
-1
0
1 at 261.403 kb on + strand at 261.724 kb on - strand at 261.833 kb on - strand at 262.128 kb on + strand at 262.170 kb on - strand at 262.212 kb on - strand, within MMP_RS01415 at 262.333 kb on - strand, within MMP_RS01415 at 262.353 kb on - strand, within MMP_RS01415 at 262.419 kb on + strand, within MMP_RS01415 at 262.625 kb on + strand, within MMP_RS01415 at 262.682 kb on - strand, within MMP_RS01415 at 262.682 kb on - strand, within MMP_RS01415 at 262.682 kb on - strand, within MMP_RS01415 at 262.729 kb on + strand at 262.734 kb on - strand at 262.748 kb on - strand at 262.762 kb on - strand at 262.789 kb on - strand at 262.790 kb on + strand at 262.793 kb on - strand at 262.796 kb on + strand at 262.824 kb on - strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.828 kb on - strand at 262.828 kb on - strand at 262.834 kb on - strand at 262.835 kb on + strand at 262.839 kb on + strand at 262.849 kb on - strand at 262.926 kb on - strand at 262.927 kb on + strand at 263.020 kb on - strand at 263.020 kb on - strand at 263.069 kb on - strand at 263.072 kb on - strand at 263.427 kb on + strand at 263.454 kb on - strand at 263.476 kb on + strand at 263.486 kb on - strand at 263.618 kb on - strand, within MMP_RS01420 at 263.621 kb on + strand, within MMP_RS01420 at 263.681 kb on + strand at 263.706 kb on - strand at 263.732 kb on - strand at 263.736 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 261,403 + -1.6 261,724 - -2.5 261,833 - +0.0 262,128 + -0.1 262,170 - +0.4 262,212 - MMP_RS01415 0.13 +0.3 262,333 - MMP_RS01415 0.33 +0.2 262,353 - MMP_RS01415 0.36 +0.1 262,419 + MMP_RS01415 0.47 +0.5 262,625 + MMP_RS01415 0.80 +0.2 262,682 - MMP_RS01415 0.89 +0.6 262,682 - MMP_RS01415 0.89 +0.0 262,682 - MMP_RS01415 0.89 +0.2 262,729 + -0.2 262,734 - +0.1 262,748 - +0.1 262,762 - -0.3 262,789 - -0.1 262,790 + -0.3 262,793 - +0.5 262,796 + -0.0 262,824 - +0.2 262,825 + +0.2 262,825 + +0.1 262,828 - +0.5 262,828 - +0.2 262,834 - -0.9 262,835 + -0.0 262,839 + -0.2 262,849 - +0.2 262,926 - -0.2 262,927 + +0.3 263,020 - -0.4 263,020 - +0.7 263,069 - -0.2 263,072 - +0.2 263,427 + +0.3 263,454 - +0.3 263,476 + +0.0 263,486 - -0.0 263,618 - MMP_RS01420 0.74 -0.0 263,621 + MMP_RS01420 0.76 +0.6 263,681 + +0.1 263,706 - -0.6 263,732 - -0.2 263,736 + +0.2
Or see this region's nucleotide sequence